不同培养基和生长调节剂对细叶小羽藓生长发育及愈伤组织诱导的影响
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摘要
对淮河上游南湾湖周边的细叶小羽藓(Haplocladium microphyllum)的孢子生长发育进行观察,发现细叶小羽藓孢子无休眠现象,孢子接种2天左右萌发;扩大培养实验结果表明,细叶小羽藓无菌原丝体在1/2 MS培养基上置于20℃±2℃、12 h光照/12 h黑暗条件培养,产生配子体最多,且配子体长势最好,可以获得大量无菌材料;细叶小羽藓愈伤组织诱导实验显示,形成愈伤组织的最佳培养基为添加1%葡萄糖+1.5 mg/L6-BA+0.25 mg/L 2,4-D的MS培养基.
Based on observation of the growth and development of spores of Haplocladium microphyllum in the south of Nanwan lake of Xinyang,it was found that there was no dormancy in the sphagnum spore,and the spore inoculation was germinated about 2 days. Expand training experimental results showed that the fine Haplocladium microphyllum sterile protonema moss on the 1/2 of the MS culture medium at 20 ℃ ±2 ℃,12 h of light/dark conditions to cultivate,produce new stem leaf body up,and the stem leaf do best,get a lot of aseptic materials; The experimental results showed that the optimal medium for the formation of callus was 1% glucose + 1.5 mg/L 6-BA+0.25 mg/L 2,4-D MS medium.
Based on observation of the growth and development of spores of Haplocladium microphyllum in the south of Nanwan lake of Xinyang,it was found that there was no dormancy in the sphagnum spore,and the spore inoculation was germinated about 2 days. Expand training experimental results showed that the fine Haplocladium microphyllum sterile protonema moss on the 1/2 of the MS culture medium at 20 ℃ ±2 ℃,12 h of light/dark conditions to cultivate,produce new stem leaf body up,and the stem leaf do best,get a lot of aseptic materials; The experimental results showed that the optimal medium for the formation of callus was 1% glucose + 1.5 mg/L 6-BA+0.25 mg/L 2,4-D MS medium.
引文
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[15]丁华娇,冯玉.苔藓植物的园林应用[J].浙江林业,2013(8):34-35.DING Huajiao,FENG Yu.Garden application of bryophytes[J]. Zhejiang forestry,2013(8):34-35.
[16]康媛,白晓珊,卜兆君,等.烟气对泥炭藓孢子萌发影响的模拟研究[J/OL].应用生态学报.https://doi.org/10.13287/j.1001-9332.201902.035.KANG Yuan,BAI Xiaoshan,BU Zhaojun et al. Simulated study on the effect of smoke on Sphagnum spore germination[J/OL].Chinese Journal of Applied Ecology.https://doi.org/10.13287/j.1001-9332.201902.035.
[17]黄士良,金红霞,王碧雪,等.不同培养基质和p H值对金灰藓孢子萌发与原丝体发育的影响[J].中国园艺文摘,2017(1):14-16.HUANG Shiliang,JIN Hongxia,WANG Bixue,et al. Influence of different culture medium and p H values on spore germi-nation and protonema growth of Pylaisiellapolyantha[J]. Chinese Horticulture Abstracts,2017(1):14-16.
[18]吴小凤,胡若洋,李学东.仙鹤藓的孢子萌发及愈伤组织诱导[J].植物学报,2013,48(6):651-657.WU Xiaofeng,HU Ruoyang,LI Xuedong. Spore germination and callus induction of Atrichum undulatum[J]. Chin BullBot,2013,48(6):651-657.
[2]朱亚利,饶本强,余亚军,等.苔藓水提液对玉米和小麦种子萌发的化感效应[J].信阳师范学院学报(自然科学版),2018,31(2):203-207.ZHU Yali,RAO Benqiang,YU Yajun,et al. Effect of the extracting liquoron from bryophytes on the germination of maizeand wheat seeds[J].Journal of Xinyang Normal University(Natural Science Edition),2018,31(2):203-207.
[3]高永超,沙伟,张晗.苔藓植物的组织培养[J].植物生理学通迅,2002,38(6):607-610.GAO Yongchao,SHA Wei,ZHANG Han. Tissue culture of bryophytes[J]. Phytophysiological Communication,2002,38(6):607-610.
[4]黄士良.侧蒴藓类植物孢子萌发与原丝体发育研究[D].石家庄:河北师范大学,2009.HUANG Shiliang.Study on the spore germination and protonnma development of pleurocarpous mosses[D].Shijiazhuang:He-bei Normal University,2009.
[5]田桂泉,张萍.干旱区固定沙丘结皮层藓类植物繁殖生物学特性研究[J].河套大学学报,2004,22(1):553-558.TIAN Guiqian,ZHANG Ping.Study on reproductive biological characteristics of mosses in fixed dune nodules in arid area[J].Journal of Hetao University,2004,22(1):553-558.
[6]陈文佳.细叶小羽藓快繁体系及对逆境的生理响应研究[D].杭州:浙江农业大学,2012.CHEN Wenjia.Study on rapid propagation system and physiological response to stress of Haplocladium microphyllum[D].Han-gzhou:Zhejiang Agricultural University,2012.
[7]刘晓红.细胞分裂素、氯丙嗪对葫芦藓发育过程的影响[J].西南师范大学学报(自然科学版),1998,23(4):476-480.LIU Xiaohong.Effects of cytokinin and chlorpromazine on the development of bryophytes[J].Journal of Southwest China Nor-mal University(Natural Science Edition),1998,23(4):476-480.
[8]张朝晖,刘宁,钟本固,等.培养基p H值对苔藓孢子萌发及原丝体生长的影响[J].隐花植物,1997,13:57-62.ZHANG Zhaohui,LIU Ning,ZHONG Bengu,et al.Effects of p H value on mosses spore germination and protonema growth[J].Cryptoflora,1997,13:57-62.
[9]魏志颖,沙伟,张梅娟.山墙藓组织培养条件研究[J].北方园艺,2014(18):114-117.WEI Zhiying,SHAWei,ZHANG Meijuan. Study on tissue culture conditions of bryophytes[J]. Northern Horticulture,2014,(18):114-117.
[10]陈蓉蓉,刘宁,杨松,等. Ca2+浓度对黔灵山喀斯特生境中几种苔藓植物生长的影响[J].贵州师范大学学报(自然科学版),1998,16(1):4-7.CHEN Rongrog,LIU Ning,YANG Song,et al.Effects of concentration of Ca2+on the growth of several bryophytes in karst hab-itat of mount qianling[J].Journal of Guizhou Normal University(Natural Sclence),1998,16(1):4-7.
[11] MACHLIS L,DOYLE W T.Submerged growth of pure cultures of the liverwort Sphaerocarpos donnellii[J]. Physiol Plant,1962,15:351-353.
[12] SOKAL I,KUTA E,PRZYWARA L. Callus induction and gametophyte regeneration in moss cultures[J].Acta Biol CracovBot,1997,39:35-42.
[13]吴玉环,程国栋,高谦.苔藓植物的生态功能及在植被恢复与重建中的作用[J].中国沙漠,2003,23(3):215-220.WU Yuhuan,CHENG Guodong,GAO Qian.The ecological function of bryophytes and their role in vegetation restoration andreconstruction[J].China sand desert,2003,23(3):215-220.
[14]汪庆,张光宁,贺善安.苔藓植物在园林中的应用前景[J].中国园林.1999(6):25-26.WANG Qing,ZHANG Guangning,HE Shanan.Application prospect of bryophytes in gardens[J]. Classical Chinese garden,1999(6):25-26.
[15]丁华娇,冯玉.苔藓植物的园林应用[J].浙江林业,2013(8):34-35.DING Huajiao,FENG Yu.Garden application of bryophytes[J]. Zhejiang forestry,2013(8):34-35.
[16]康媛,白晓珊,卜兆君,等.烟气对泥炭藓孢子萌发影响的模拟研究[J/OL].应用生态学报.https://doi.org/10.13287/j.1001-9332.201902.035.KANG Yuan,BAI Xiaoshan,BU Zhaojun et al. Simulated study on the effect of smoke on Sphagnum spore germination[J/OL].Chinese Journal of Applied Ecology.https://doi.org/10.13287/j.1001-9332.201902.035.
[17]黄士良,金红霞,王碧雪,等.不同培养基质和p H值对金灰藓孢子萌发与原丝体发育的影响[J].中国园艺文摘,2017(1):14-16.HUANG Shiliang,JIN Hongxia,WANG Bixue,et al. Influence of different culture medium and p H values on spore germi-nation and protonema growth of Pylaisiellapolyantha[J]. Chinese Horticulture Abstracts,2017(1):14-16.
[18]吴小凤,胡若洋,李学东.仙鹤藓的孢子萌发及愈伤组织诱导[J].植物学报,2013,48(6):651-657.WU Xiaofeng,HU Ruoyang,LI Xuedong. Spore germination and callus induction of Atrichum undulatum[J]. Chin BullBot,2013,48(6):651-657.