有氧运动干预对衰老大鼠学习记忆能力及小脑生长相关蛋白-43表达的影响
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摘要
目的:探讨衰老过程中进行有氧运动干预对大鼠学习记忆能力及小脑神经生长相关蛋白-43(growth associated protein-43,GAP-43)表达的影响。方法:45只成年雄性SD大鼠随机分为生理盐水对照组(Na组,n=15)、有氧运动干预组(S组,n=15)和衰老对照组(N组,n=15)。S组进行6周的衰老造模同时进行有氧运动干预,N组大鼠进行6周的衰老造模,Na组大鼠注射6周的生理盐水进行对照实验。三组大鼠造模结束后分别进行7天的Morris水迷宫实验测试大鼠的学习记忆能力,Morris水迷宫结束后24h内取大鼠的小脑,利用实时PCR法检测小脑内GAP-43 mRNA表达水平,Western-blotting法检测小脑GAP-43蛋白含量。结果:Morris水迷宫定位航线实验当中:第2天和第1天相比,第3天和第2天相比,Na组、N组和S组大鼠平均潜伏期均缩短且存在显著性差异(P<0.01);第4天和第3天相比,N组大鼠平均潜伏期存在显著性差异(P<0.01),Na组和S组平均潜伏期无显著性差异(P>0.05);第5天和第4天相比,第6天和第5天相比,Na组、N组和S组大鼠平均潜伏期无显著性差异(P>0.05)。Morris水迷宫空间探索实验结果显示:Na组和S组穿越原平台位置次数多于N组且存在显著性差异(P<0.01)。实时PCR实验和Western-blotting结果显示:Na组和S组大鼠小脑内GAP-43表达量皆高于N组大鼠且存在显著性差异(P<0.01)。结论:在衰老过程中进行有氧运动干预在一定程度上可以促进脑衰老大鼠的学习记忆能力和小脑内GAP-43的表达,这可能是有氧运动延缓脑衰老学习记忆能力下降的分子机制之一。
Objective: To investigate the effects of aerobic exercise intervention on learning and memory ability and expression of growth associated protein-43(GAP-43) in the cerebellum in aging rats.Method: Forty-five male adult SD(Sprague-Dawley) rats were randomly divided into normal saline control group(Na group, n=15), aerobic exercise intervention group(S group, n=15) and aged control group(N group,n=15). Group S performed 6 weeks of aging modeling while performing aerobic exercise intervention,Group N rats were subjected to aging modeling for 6 weeks, and Na group rats were injected with physiological saline for 6 weeks for control experiments. Rats' learning and memory ability was tested by Morris water maze for 7 days after the completion of each model. Rat's cerebellum was taken within 24 hours after Morris water maze was completed. The expression of GAP-43 mRNA in cerebellum was detected by real-time PCR, and the content of GAP-43 protein in cerebellum was measured by Western-blotting method.Result: In the Morris water maze locator route experiment, compared to the second day and the first day, compared to the third day and the second day, the average latency of the Na group, the N group and the S group was shortened and there was a significant difference(P<0.01); compared with the fourth day and the third day, the average latency of group N was significantly different(P<0.01), and there was no significant difference in average latency between group Na and group S(P>0.05); compared to the fifth day and the fourth day, compared to the sixth day and the fifth day, there was no significant difference in the average latency between the Na group, the N group, and the S group(P>0.05). The Morris water maze space exploration experiment results show that: Na group and S group crossing the original platform position more than N group and there is a significant difference(P<0.01). Real-time PCR and Western-blotting results showed that the expression of GAP-43 in the cerebellum of Na group and S group was higher than that of N group rats and there was a significant difference(P<0.01).Conclusion: Aerobic exercise intervention during the aging process can promote the learning and memory ability and the expression of GAP-43 in the cerebellum of rats in a certain extent, which may be one of the molecular mechanisms of aerobic exercise retarding the decline of learning and memory abilities of brain aging.
Objective: To investigate the effects of aerobic exercise intervention on learning and memory ability and expression of growth associated protein-43(GAP-43) in the cerebellum in aging rats.Method: Forty-five male adult SD(Sprague-Dawley) rats were randomly divided into normal saline control group(Na group, n=15), aerobic exercise intervention group(S group, n=15) and aged control group(N group,n=15). Group S performed 6 weeks of aging modeling while performing aerobic exercise intervention,Group N rats were subjected to aging modeling for 6 weeks, and Na group rats were injected with physiological saline for 6 weeks for control experiments. Rats' learning and memory ability was tested by Morris water maze for 7 days after the completion of each model. Rat's cerebellum was taken within 24 hours after Morris water maze was completed. The expression of GAP-43 mRNA in cerebellum was detected by real-time PCR, and the content of GAP-43 protein in cerebellum was measured by Western-blotting method.Result: In the Morris water maze locator route experiment, compared to the second day and the first day, compared to the third day and the second day, the average latency of the Na group, the N group and the S group was shortened and there was a significant difference(P<0.01); compared with the fourth day and the third day, the average latency of group N was significantly different(P<0.01), and there was no significant difference in average latency between group Na and group S(P>0.05); compared to the fifth day and the fourth day, compared to the sixth day and the fifth day, there was no significant difference in the average latency between the Na group, the N group, and the S group(P>0.05). The Morris water maze space exploration experiment results show that: Na group and S group crossing the original platform position more than N group and there is a significant difference(P<0.01). Real-time PCR and Western-blotting results showed that the expression of GAP-43 in the cerebellum of Na group and S group was higher than that of N group rats and there was a significant difference(P<0.01).Conclusion: Aerobic exercise intervention during the aging process can promote the learning and memory ability and the expression of GAP-43 in the cerebellum of rats in a certain extent, which may be one of the molecular mechanisms of aerobic exercise retarding the decline of learning and memory abilities of brain aging.
引文
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[19]徐榛.突触相关蛋白SYN及GAP-43在维生素D缺乏致大鼠学习记忆功能障碍的作用研究[D].南宁:广西医科大学,2016.
[20]王富鸿,李雪,邓文骞,等.过度负荷运动对大鼠学习记忆能力及海马生长相关蛋白的影响[J].武汉体育学院学报,2015,49(2):72-77
[21]田琪,王洋,刘正琦,等.慢性铅暴露对大鼠GAP-43蛋白表达和学习记忆影响[J].中国公共卫生,2011,27(1):94-95.
[22]金其贯,吴尚琳,王云峰,等.低氧和运动训练对大鼠学习记忆能力的影响及其与海马突触可塑性的关系[J].体育科学,2015,35(1):54-59.
[23]李捷,寇现娟,刘醒然,等.运动激活自噬改善D-半乳糖诱导的衰老大鼠学习记忆能力[C].第四届全国运动生理与生物化学学术会议,无锡,2016.
[24]黎润.不同负荷运动对大鼠小脑GAP-43基因表达及学习记忆能力的影响[D].成都:成都体育学院,2013.
[25]樊振勇,程丽娜,徐琳峰,等.运动训练对血管痴呆大鼠学习记忆能力及大脑皮质区生长相关蛋白-43表达的影响[J].中国康复医学杂志,2010,25(6):514-518.
[26]王富鸿.长期不同负荷运动对大鼠学习记忆能力及海马GAP-43基因表达的影响[D].成都:成都体育学院,2012.
[2]崔建梅,杨文艳,药宏慧,等.有氧运动对慢性应激大鼠空间学习记忆及海马齿状回BDNF表达的影响[J].首都体育学院学报,2018,30(1):78-82+87.
[3]周娜,赵雅宁,李佳宁,等.不同强度炮台运动对脑缺血大鼠学习记忆能力及细胞周期蛋白表达的影响[J].中国康复医学杂志,2017,32(6):654-660.
[4]于龙川.神经生物学[M].北京:北京大学出版社,2012:380-389.
[5]Yang L,Zhanhua L,Jing L,et al.Downregulation of caveolin-contributes to the synaptic plasticity deficit in the hippocampus of aged rats[J].neural Regen Res,2013,29(8):2725-2733.
[6]栾海云,李娜,王桂华,等.游泳运动对D-半乳糖致衰老小鼠学习记忆和胆碱能系统功能的影响[J].中国运动医学杂志,2016,35(1):36-39.
[7]亢依婷,赵新娟,张静,等.神经细胞粘附分子对脑学习记忆功能的影响及其运动干预展望[J].辽宁体育科技,2018,40(1):36-39.
[8]董军涛,林阳阳,燕铁斌,等.不同形式的运动训练对血管痴呆大鼠学习记忆能力及海马区突触可塑性的影响[J].中国康复医学杂志,2016,31(7):716-722.
[9]孙树敏,刘浩.神经生长相关蛋白与突触可塑性[J].天津医科大学学报,2007,3(1):118-121.
[10]吴银侠,陈新胜,袁小燕,等.参芎葡萄糖注射液对脊髓损伤后运动功能恢复及GAP-43和NF200表达的影响[J].中国现代医学杂志,2014,24(2):44-49.
[11]宋晨晨.美金刚和瑞舒伐他汀对血管痴呆大鼠血管再生及神经功能的影响[D].天津:天津医科大学,2015.
[12]孙思琪.PGRN通过促进海马神经再生改善脑缺血后的焦虑情绪及学习记忆能力[D].西安:陕西师范大学,2018.
[13]科学技术部办公厅.关于发布(关于善待实验动物的指导性意见)的通知.国科发财字-2006-398号-S-.2006年10月.
[14]雷鸣.D-半乳糖致脑衰老机制的研究[D].南京:南京医科大学,2008.
[15]马碧,王文敏.小脑在高级认知功能中作用的研究进展[J].医学综述,2013,19(7):1262-1264.
[16]唐红梅,董军.小脑中的LTD在学习记忆中的作用及产生机制[J].陕西医学杂志,2006,35(12):1681-1683.
[17]Korshunova I,Mosevitsky M.Role of the growth-associated protein GAP-43 in NCAM-mediated neurite outgrowth[J].Adv Exp Med Biol,2009,663:169-182.
[18]Emery DL,Royo NC,Fisher I,et al.Plasticity following Injury to the Adult Central Nervous System:is Recapitulation of a Developmental State Worth Promoting[J].J Neurotrauma,2003,20(12):1271-1292.
[19]徐榛.突触相关蛋白SYN及GAP-43在维生素D缺乏致大鼠学习记忆功能障碍的作用研究[D].南宁:广西医科大学,2016.
[20]王富鸿,李雪,邓文骞,等.过度负荷运动对大鼠学习记忆能力及海马生长相关蛋白的影响[J].武汉体育学院学报,2015,49(2):72-77
[21]田琪,王洋,刘正琦,等.慢性铅暴露对大鼠GAP-43蛋白表达和学习记忆影响[J].中国公共卫生,2011,27(1):94-95.
[22]金其贯,吴尚琳,王云峰,等.低氧和运动训练对大鼠学习记忆能力的影响及其与海马突触可塑性的关系[J].体育科学,2015,35(1):54-59.
[23]李捷,寇现娟,刘醒然,等.运动激活自噬改善D-半乳糖诱导的衰老大鼠学习记忆能力[C].第四届全国运动生理与生物化学学术会议,无锡,2016.
[24]黎润.不同负荷运动对大鼠小脑GAP-43基因表达及学习记忆能力的影响[D].成都:成都体育学院,2013.
[25]樊振勇,程丽娜,徐琳峰,等.运动训练对血管痴呆大鼠学习记忆能力及大脑皮质区生长相关蛋白-43表达的影响[J].中国康复医学杂志,2010,25(6):514-518.
[26]王富鸿.长期不同负荷运动对大鼠学习记忆能力及海马GAP-43基因表达的影响[D].成都:成都体育学院,2012.