基于两株菌的复合菌处理油田采出水效果评价
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摘要
为了降低油田采出水中聚丙烯酰胺和石油烃质量浓度,提高采出水处理效果,将石油烃降解菌株D与聚丙烯酰胺降解菌株H进行复配,考察了复合菌接种量、复配比例对聚丙烯酰胺和石油烃的降解效果。结果表明:在合成培养基中,当复合菌接种量体积分数为5%时,降解效果最好;在此基础上,当D和H的复配比例为3∶1和3∶2时,7 d聚丙烯酰胺降解率分别为57.79%和60.76%,石油烃降解率分别为70.39%和65.05%,这两个复配比例时,复合菌处理效果均优于单菌的处理效果;在应用于油田采出水处理时,当复配比例为3∶1和3∶2时,7 d聚丙烯酰胺降解率分别为50.51%和54.26%,石油烃降解率分别为64.24%和66.18%,降解效果均较好且复配比例3∶2时降解效果优于3∶1时降解效果。当复配比例为3∶2、复合菌接种量体积分数为5%时,应用于油田采出水处理,聚丙烯酰胺和石油烃降解效果最佳。
In order to reduce the mass concentrations of the polyacrylamide & petroleum hydrocarbon in the oilfield produced water and improve the water treated effect, Degradation Strain D of the petroleum hydrocarbon and Degradation Strain H of the polyacrylamide were combined, the degradation effects of the inoculation quantity and compatibility ratio to the polyacrylamide and petroleum hydrocarbon were investigated.The results show that the degradation effects are the best when the inoculation volume fraction of the compound bacterium is 5% in the synthetic culture medium; the degradation rates of 7 d polyacrylamide is 57.79% and 60.76% respectively when the ratios of D to H are 3∶1 and 3∶2, while the degradation rates of the petroleum hydrocarbon are 70.39% and 65.05% respectively.For the two compatibility ratios, the treated effects of both compound bacteria are better than that of mono bacteria;when the compound bacteria are used for the oilfield water treatment, the degradation rates of 7 d polyacrylamide are 50.51% and 54.26% respectively, when the compatibility ratios are 3∶1 and 3∶2, while the degradation rates of the petroleum hydrocarbon are 64.24% and 66.18% respectively, the degraded effects of them are much better and moreover the latter is better than the former.When the ratio is 3∶2 and the inoculation volume fraction of the compound bacteria is 5%, the degraded effects of both the polyacrylamide and petroleum hydrocarbon are the best when applied in the oilfield water treatment.
In order to reduce the mass concentrations of the polyacrylamide & petroleum hydrocarbon in the oilfield produced water and improve the water treated effect, Degradation Strain D of the petroleum hydrocarbon and Degradation Strain H of the polyacrylamide were combined, the degradation effects of the inoculation quantity and compatibility ratio to the polyacrylamide and petroleum hydrocarbon were investigated.The results show that the degradation effects are the best when the inoculation volume fraction of the compound bacterium is 5% in the synthetic culture medium; the degradation rates of 7 d polyacrylamide is 57.79% and 60.76% respectively when the ratios of D to H are 3∶1 and 3∶2, while the degradation rates of the petroleum hydrocarbon are 70.39% and 65.05% respectively.For the two compatibility ratios, the treated effects of both compound bacteria are better than that of mono bacteria;when the compound bacteria are used for the oilfield water treatment, the degradation rates of 7 d polyacrylamide are 50.51% and 54.26% respectively, when the compatibility ratios are 3∶1 and 3∶2, while the degradation rates of the petroleum hydrocarbon are 64.24% and 66.18% respectively, the degraded effects of them are much better and moreover the latter is better than the former.When the ratio is 3∶2 and the inoculation volume fraction of the compound bacteria is 5%, the degraded effects of both the polyacrylamide and petroleum hydrocarbon are the best when applied in the oilfield water treatment.
引文
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[2]苏党生.油田水处理技术发展及展望探析[J].化工中间体,2015,11(5):128.SU Dangsheng.The development of oilfield water treatment technology and future prospect[J].Chemical Intermediates,2015,11(5):128.
[3]毛翠玲.微生物处理技术在油田水处理中的应用[J].科技创新导报,2012,12(23):87.MAO Cuiling.Application of microbial treatment technology in oil field water treatment[J].Science and Technology Innovation Herald,2012,12(23):87.
[4]杨波,张丽媛,冯利东.固定化漆酶对水中邻苯二胺的去除研究[J].安全与环境学报,2012,12(2):32-36.YANG Bo,ZHANG Liyuan,FENG Lidong.Research on the removal of o-phenylenediamine in water by immobilized laccase[J].Journal of Safety and Environment,2012,12(2):32-36.
[5]赵胜绪,岳湘安,张立娟,等.部分水解聚丙烯酰胺溶液在孔喉模型中机械降解的主控因素[J].油气地质与采收率,2018,25(6):184-188.ZHAO Shengxu,YUE Xiang’an,ZHANG Lijuan,et al.Main controlling factors of mechanical degradation of HPAM in pore-throat model[J].Petroleum Geology and Recovery Efficiency,2018,25(6):184-188.
[6]刘江红,徐瑞丹,潘洋,等.混合菌降解含聚污水的最佳条件试验研究[J].安全与环境学报,2013,12(3):14-17.LIU Jianghong,XU Ruidan,PAN Yang,et al.Experimental study of the optimal conditions for degrading HPAM-containing sewage by using the mixed bacteria[J].Journal of Safety and Environment,2013,12(3):14-17.
[7]滕立勇,宋华,蒋肇标,等.污油泥在地层中运移规律研究[J].特种油气藏,2018,25(6):159-164.TENG Liyong,SONG Hua,JIANG Zhaobiao,et al.Migration of oil sludge in formation[J].Special Oil & Gas Reservoirs,2018,25(6):159-164.
[8]周金葵,王大威,廖明清,等.一株石油烃降解菌的筛选及性能评价[J].大庆石油地质与开发,2007,26(6):119-123.ZHOU Jinkui,WANG Dawei,LIAO Mingqing,et al.Screening and performance evaluation of one petroleum hydrocarbon degrading bacterium[J].Petroleum Geology & Oilfield Development in Daqing,2007,26(6):119-123.
[9]卞立红,郎亚军,张奕婷,等.油田采出水中聚丙烯酰胺的生物降解[J].大庆石油地质与开发,2018,37(4):114-117.BIAN Lihong,LANG Yajun,ZHANG Yiting,et al.Biodegradation of the polyacrylamide in the oilfield produced water[J].Petroleum Geology & Oilfield Development in Daqing,2018,37(4):114-117.
[10]孙远军.环境中石油污染物的检测与分析方法研究[J].工业用水与废水,2013,44(2):86-89.SUN Yuanjun.Detection and analysis method for petroleum pollutants in environment[J].Industrial Water and Wastewater,2013,44(2):86-89.
[11]高玉格,张忠智,高伯南,等.一组混合菌降解水解聚丙烯酰胺和石油的研究[J].工业水处理,2008,28(9):62-65.GAO Yuge,ZHANG Zhongzhi,GAO Bonan,et al.Research on the degradation of HPAM and oil with a group of mixed microbes[J].Industrial Water Treatment,2008,28(9):62-65.