低温β-甘露聚糖酶菌株的筛选鉴定及产酶条件的研究
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摘要
以海泥、海水为样品筛选产低温β-甘露聚糖酶的菌株,得到产酶高的细菌1株,命名为WYQ-01。菌株为杆菌,长2μm、宽0.6μm,革兰氏阴性。菌株形成的单菌落表面光滑、湿润、易于挑取、边缘整齐、边缘与中间厚薄不一、不透明、乳白色、直径大小为0.8 cm。产生的低温β-甘露聚糖酶为胞外酶,透明圈直径大小为3 cm,透明圈与菌落直径比为3:0.8。采用单因素实验对其产酶条件进行研究。结果表明:发酵时间为28 h,温度为25℃,pH 7.0,装液量100 mL,转速160 r/min和接种量3%,酶活高达2275.265 U/mL。
Using sea mud and sea water as the samples,a strain producing cold-adapted β-mannanase with high enzyme production was obtained,named as bacterium WYQ-01.It was bacillus,2 μm long,0.6 μm wide,gram-negative.The colony was smooth surface,humid,easy to pick,neat edges,with different thickness between intermediates and edges,opaque,ivory,its diameter was 0.8 cm.The cold-adapted β-mannanase was an extracellular enzyme,Diameter of the transparent circle was 3 cm,and the ratio of transparent circle to colony diameter was 3:0.8.The enzyme production conditions of the strain producing cold-adapted β-mannanase by single-factor experiments were determined as followed:fermentation time 28 h,temperature
Using sea mud and sea water as the samples,a strain producing cold-adapted β-mannanase with high enzyme production was obtained,named as bacterium WYQ-01.It was bacillus,2 μm long,0.6 μm wide,gram-negative.The colony was smooth surface,humid,easy to pick,neat edges,with different thickness between intermediates and edges,opaque,ivory,its diameter was 0.8 cm.The cold-adapted β-mannanase was an extracellular enzyme,Diameter of the transparent circle was 3 cm,and the ratio of transparent circle to colony diameter was 3:0.8.The enzyme production conditions of the strain producing cold-adapted β-mannanase by single-factor experiments were determined as followed:fermentation time 28 h,temperature
引文
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[16]张云光,韦玉琴,卢丽萍,等.产β-甘露聚糖酶海洋微生物的筛选[J].食品与生物,2011,3(2):3-5.
[2]A A Klyosov,G S Dotsenko,S W A Hinz,et al.Structural features ofβ-(1,4)-D-GalactoManna's of plant origin as a probe forβ-(1,4)-mannanase polymeric substrate specificity[J].Carbohydrate Research,2012,352:65-69.
[3]Sudip,Regmi PradeepG C,Yun Hee Choia,et al.Amulti-tolerant low molecular weight mannanase from Bacillus sp.CSB39 and its compatibility as an industrial biocatalystSudip[J].Enzyme and Microbial Technology,2016,92:76-85.
[4]Jung-Jin Lee,Jakyeom Seo,Jae Keun Jung,et al.Effects ofβ-mannanase supplementation on growth performance,nutrient digestibility,and nitrogen utilization of Koreannative goat[J].Livestock Science,2014,169:83-87.
[5]秦玲丽,李雪晴,崔堂兵.产β-甘露糖酶菌株的筛选、鉴定及酶学性质研究[J].食品工业科技,2016,37(24):249-254.
[6]张建新,韩科芳,刘起丽,等.产β-甘露聚糖酶内生菌最适发酵条件的优化[J].饲料工业,2012,33(14):89-94.
[7]Bengu Ozturk,Deniz Cekmecelioglu,Zumrut BegumOgel.Optimal conditions for enhancedβ-mannanase production by recombinant Aspergillus sojae[J].Journal of Molecular Catalysis B,2010:135-139.
[8]Saroj Ahirwara,Hemant Soni a,Hemant Kumar Rawata,et al.Production optimization and functional characterization of thermostableβ-mannanase from Malbranchea cinnamomea NFCCI 3724 and its applicability in mannotetraose(M 4)generation[J].Journal of Chemical Engineering's,2016,63,344-353.
[9]成莉凤,冯湘沅,段盛文,等.新型β-甘露聚糖酶制备葡甘寡糖工艺优化[J].食品科学,2016,37(6):34-38.
[10]张晓龙,肖静,王瑞明.毕赤酵母高密度发酵产β-甘露聚糖酶的工艺优化[J].湖北农业科学,2015,54(23):5978-5983.
[11]Pijug Summpunn,Suttidarak Chaijan,Duangnate Isarangkul,et al.Characterization gene cloning and heterogonous expression of β-mannanase from a thermophilic Bacillus subtilus[J].The Journal of marinebiology,2011,49(1):86-93.
[12]Jian-Wen Huang,Chun-Chi Chen,Chun-Hsiang Huang,et al.Improving the specific activity of β-mannanase from Aspergillus niger BK01 by structure-based rational design[J].Biochemical ET Biophysical Acta,2014,26(6):663-669.
[13]Taicheng Zhua,Hongbing Sun,Pengfei Li,et al.Constitutive expression of alkaline-mann-anase in recombinant Pichia pastoris[J].J Marinebiol,2014,49:2025-2029.
[14]Yutaka Tamaru,Tatsu Morishita,et al.Cloning DNASequencing and Expression of the β-1,4-Mannana Gene from a Marine Bacterium,Vibrio sp.Strain MA-1318[J].Journal of Fermentation and Bioengineering,2007,83(2):201-205.
[15]O Politz M.Krah K K,Thomsen R Borriss.A highly thermostable endo-(1,4)-β-mannanase from the marine bacterium Rhodothermus marinus[J].Application Microbiology technology,2000,53(2):715-721.
[16]张云光,韦玉琴,卢丽萍,等.产β-甘露聚糖酶海洋微生物的筛选[J].食品与生物,2011,3(2):3-5.