矿泉水中微生物检测能力验证结果与分析
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摘要
为了提高实验室对矿泉水中微生物的检测能力,参加了由中国检验检疫科学研究院测试评价中心(ACAS)组织的矿泉水中微生物检测能力验证(ACAS-PT550/551/552/553)。依据能力验证作业指导书和GB 8538—2016《饮用天然矿泉水检验方法》对样品进行大肠菌群、铜绿假单胞菌、粪链球菌及产气荚膜梭菌测定,采用全自动微生物生化鉴定系统鉴定可疑菌落。结果显示,所有项目的∣统计量(Z)∣均<2.0,结果均为满意。实验室能很好地应用GB 8538—2016《饮用天然矿泉水检验方法》开展微生物检测。
To improve the ability of laboratory to detect microorganism in mineral water, the laboratory Participated in the Mineral Water Microbiological Proficiency Testing(ACAS-PT550/551/552/553)organized by Analysis Capability Assessment System of CAIQ(ACAS). According to the working instruction of proficiency testing and national standard GB 8538—2016 Test Method for Drinking Natural Mineral Water, coliforms,pseudomonas aeruginosa, enterococcus faecalis and clostridium perfringens were tested. At the same time,the isolated suspicious colonies were identified by automatic microbial and biochemical identification system. The results showed that the absolute value of Z scores for all items were less than 2.0 and all tests have achieved satisfactory results. The laboratory can apply the national standard GB 8538—2016"Test Method for Drinking Natural Mineral Water" well in microbiological examination.
To improve the ability of laboratory to detect microorganism in mineral water, the laboratory Participated in the Mineral Water Microbiological Proficiency Testing(ACAS-PT550/551/552/553)organized by Analysis Capability Assessment System of CAIQ(ACAS). According to the working instruction of proficiency testing and national standard GB 8538—2016 Test Method for Drinking Natural Mineral Water, coliforms,pseudomonas aeruginosa, enterococcus faecalis and clostridium perfringens were tested. At the same time,the isolated suspicious colonies were identified by automatic microbial and biochemical identification system. The results showed that the absolute value of Z scores for all items were less than 2.0 and all tests have achieved satisfactory results. The laboratory can apply the national standard GB 8538—2016"Test Method for Drinking Natural Mineral Water" well in microbiological examination.
引文
[1]CNAS-RL02:2016能力验证规则[S].
[2]席静,张思群,刘静宇,等.论能力验证活动对实验室能力建设的作用和意义[J].中国卫生检验杂志,2011,21(6):1576-1578.
[3]GB 8538-2016食品安全国家标准饮用天然矿泉水检验方法[S].
[4]GB 19489-2008实验室生物安全通用要求[S].
[5]褚福敏,许燕,逯南南,等.酶底物法和滤膜法检验饮用水中铜绿假单胞菌方法的比较[J].中国给水排水,2017,33(22):131-134.
[6]李金霞,程池,郭立芸.饮用天然矿泉水中产气荚膜梭菌检测方法改良[J].中国卫生检验杂志,2010,20(8):2079.
[7]潘宝怡,吴清平,彭飞艇,等.饮用天然矿泉水中产气荚膜梭菌检测国标方法的改进[J].现代食品科技,2012,28(9):1236-1238.
[8]李婉芬,卢安婷,张淑敏,等.利用产气荚膜梭菌作为泳滩水质污染指标菌的检测方法[J].中国卫生检验杂志,2017,27(14):2026-2028.
[9]张艳超,史永刚,卫佳欢,等.3种方法检测食品中沙门菌能力验证结果分析[J].检验检疫学刊,2016,26(3):14-17.
[10]丁秀琼,杨劲,刘骁,等.3种方法检测食品中单增李斯特菌能力验证结果分析[J].检验检疫学刊,2018,28(6):15-18.
[11]高飞,白东亭,黄杰,等.化妆品中金黄色葡萄球菌的PCR快速检测[J].环境与健康杂志,2016,33(9):814-816.
[12]郑萍,毛怡心,陈西平,等.水中产气荚膜梭菌的荧光定量PCR检测法[J].环境与健康杂志,2017,34(8):698-700.
[13]张淑红,吴清平,徐晓可,等.桶装水中铜绿假单胞菌检测方法的比较[J].现代食品科技,2011,27(11):1403-1405.
[14]陈冠武,苏建晖,陈东娥,等.化妆品中铜绿假单胞菌检验方法探讨[J].检验检疫学刊,2011,21(2):16-18.
[15]张昕悦,张秀军,何聪芬,等.聚合酶链式反应技术检测化妆品中的铜绿假单胞菌[J].日用化学工业,2012,42(4):267-270.
[16]唐轶君,张建军,李晶,等.水中铜绿假单胞菌盲样考核结果分析[J].生物化工,2017,3(3):46-49.
[17]GB 17378.7-2007海洋检测规范第7部分:近海污染生态调查和生物检测[S].
[18]马群飞,林坚.GB/T 8538-2008饮用天然矿泉水检验方法存在的微生物学问题[J].中国卫生标准管理,2011,2(4):44-47.
[2]席静,张思群,刘静宇,等.论能力验证活动对实验室能力建设的作用和意义[J].中国卫生检验杂志,2011,21(6):1576-1578.
[3]GB 8538-2016食品安全国家标准饮用天然矿泉水检验方法[S].
[4]GB 19489-2008实验室生物安全通用要求[S].
[5]褚福敏,许燕,逯南南,等.酶底物法和滤膜法检验饮用水中铜绿假单胞菌方法的比较[J].中国给水排水,2017,33(22):131-134.
[6]李金霞,程池,郭立芸.饮用天然矿泉水中产气荚膜梭菌检测方法改良[J].中国卫生检验杂志,2010,20(8):2079.
[7]潘宝怡,吴清平,彭飞艇,等.饮用天然矿泉水中产气荚膜梭菌检测国标方法的改进[J].现代食品科技,2012,28(9):1236-1238.
[8]李婉芬,卢安婷,张淑敏,等.利用产气荚膜梭菌作为泳滩水质污染指标菌的检测方法[J].中国卫生检验杂志,2017,27(14):2026-2028.
[9]张艳超,史永刚,卫佳欢,等.3种方法检测食品中沙门菌能力验证结果分析[J].检验检疫学刊,2016,26(3):14-17.
[10]丁秀琼,杨劲,刘骁,等.3种方法检测食品中单增李斯特菌能力验证结果分析[J].检验检疫学刊,2018,28(6):15-18.
[11]高飞,白东亭,黄杰,等.化妆品中金黄色葡萄球菌的PCR快速检测[J].环境与健康杂志,2016,33(9):814-816.
[12]郑萍,毛怡心,陈西平,等.水中产气荚膜梭菌的荧光定量PCR检测法[J].环境与健康杂志,2017,34(8):698-700.
[13]张淑红,吴清平,徐晓可,等.桶装水中铜绿假单胞菌检测方法的比较[J].现代食品科技,2011,27(11):1403-1405.
[14]陈冠武,苏建晖,陈东娥,等.化妆品中铜绿假单胞菌检验方法探讨[J].检验检疫学刊,2011,21(2):16-18.
[15]张昕悦,张秀军,何聪芬,等.聚合酶链式反应技术检测化妆品中的铜绿假单胞菌[J].日用化学工业,2012,42(4):267-270.
[16]唐轶君,张建军,李晶,等.水中铜绿假单胞菌盲样考核结果分析[J].生物化工,2017,3(3):46-49.
[17]GB 17378.7-2007海洋检测规范第7部分:近海污染生态调查和生物检测[S].
[18]马群飞,林坚.GB/T 8538-2008饮用天然矿泉水检验方法存在的微生物学问题[J].中国卫生标准管理,2011,2(4):44-47.