施氮量对低谷蛋白水稻籽粒品质及蛋白质组分的影响
|
摘要
为探明施氮量对低谷蛋白水稻功能成分的影响,以低谷蛋白水稻D105为材料,在大田栽培条件下,设置0、90、135、180、225 kg·hm~(-2)5个施氮处理(N0-N4),研究不同氮肥水平对籽粒蛋白质组分含量动态变化及加工品质的影响,并分析了各组分蛋白比例变化。结果表明,灌浆结实期D105籽粒总蛋白含量及各组分蛋白含量随着施氮量的增加而增加,成熟期(花后35 d)籽粒清蛋白和醇溶蛋白在各施肥处理下差异均不显著,而球蛋白和谷蛋白则表现出不同的差异性,说明不同的施氮量对清蛋白和醇溶蛋白的影响较小,对球蛋白和谷蛋白的影响较大,但不改变各组分蛋白的比例;在N2(135 kg·hm~(-2))-N3(180 kg·hm~(-2))处理下,稻米的加工品质和食味品质表现良好,在N4(225 kg·hm~(-2))处理时,稻米综合品质降低。综上,实现低谷蛋白水稻D105优质的适宜施氮范围为N2-N3,建立谷蛋白相对于总蛋白的比例与施氮量的线性方程y=6E-05x2-0. 02x+39. 672 (R2=0. 999 1),计算得出最适宜施氮量为166. 67 kg·hm~(-2)。
To investigate the effect of nitrogen application amount on the functional component of low-gluten rice,D105 low-gluten rice was used as material and five nitrogen treatments were set under field cultivation conditions( 0,90,135,180,225 kg·hm~(-2)),then the effect of different nitrogen levels on the dynamic changes of protein content,processing quality of the grain were investigated and the proportion changes of each component protein were analyzed.The result showed that the total protein content and each component protein content of D105 increased with the increase of nitrogen application amount during grain filling phase. During the maturity period( 35 days after flowering),the difference between albumin and gliadin of rice grain was not significant in all fertilization treatments,while globulin and gluten showed different discrepancy,which indicated influence of different nitrogen application amount on albumin and gliadin were slight contrary with globulin and gluten. In addition,different nitrogen application did not change the proportion of component protein. The processing quality and taste quality of D105 low-gluten rice performed properly in the N2( 135 kg·hm~(-2))-N3( 180 kg·hm~(-2)) treatment. When N4( 225 kg·hm~(-2)) was treated,the integrated quality of rice decreased. In summary,the optimal nitrogen application amount for D105 low-gluten protein was between N2 and N3,and the equation of ratio of gluten to total protein with nitrogen application amount were established. The linear equation: y = 6 E-05 x2-0. 02 x + 39. 672( R2= 0. 9991). The optimum nitrogen application amount,166. 67 kg·hm~(-2),was calculated.
To investigate the effect of nitrogen application amount on the functional component of low-gluten rice,D105 low-gluten rice was used as material and five nitrogen treatments were set under field cultivation conditions( 0,90,135,180,225 kg·hm~(-2)),then the effect of different nitrogen levels on the dynamic changes of protein content,processing quality of the grain were investigated and the proportion changes of each component protein were analyzed.The result showed that the total protein content and each component protein content of D105 increased with the increase of nitrogen application amount during grain filling phase. During the maturity period( 35 days after flowering),the difference between albumin and gliadin of rice grain was not significant in all fertilization treatments,while globulin and gluten showed different discrepancy,which indicated influence of different nitrogen application amount on albumin and gliadin were slight contrary with globulin and gluten. In addition,different nitrogen application did not change the proportion of component protein. The processing quality and taste quality of D105 low-gluten rice performed properly in the N2( 135 kg·hm~(-2))-N3( 180 kg·hm~(-2)) treatment. When N4( 225 kg·hm~(-2)) was treated,the integrated quality of rice decreased. In summary,the optimal nitrogen application amount for D105 low-gluten protein was between N2 and N3,and the equation of ratio of gluten to total protein with nitrogen application amount were established. The linear equation: y = 6 E-05 x2-0. 02 x + 39. 672( R2= 0. 9991). The optimum nitrogen application amount,166. 67 kg·hm~(-2),was calculated.
引文
[1]张建奎.作物品质分析[M].重庆:西南师范大学出版社,2012.
[2]刘海,赵欢,何佳芳,等.稻米营养品质影响因素研究进展[J].贵州农业科学,2013,41(6):85-89.LIU H,ZHAO H,HE G F,et al.Advances in the influencing factors of rice nutritional quality[J].Guizhou Agricultural Sciences,2013,41(6):85-89.(in Chinese with English abstract)
[3]周丽慧,刘巧泉,张昌泉,等.水稻种子蛋白质含量及组分在品种间的变异与分布[J].作物学报,2009,35(5):884-891.ZHOU L H,LIU Q Q,ZHANG C Q,et al.Variation and distribution of seed storage protein content and composition among different rice varieties[J].Acta Agronomica Sinica,2009,35(5):884-891.(in Chinese with English abstract)
[4]张莉萍,陈少龙,孟巧霞,等.水稻低谷蛋白研究进展[J].农业科技通讯,2012(5):7-8.ZHANG L P,CHEN S L,MENG Q X,et al.Advances in the rice low-gluten protein research[J].Bulletin of Agricultural Science and Technology,2012(5):7-8.(in Chinese)
[5]蔡金洋,杨尧城.低含量谷蛋白水稻研究进展[J].农业科技通讯,2011(8):154-155.CAI J Y,YANG Y C.Advance of the low content gluten rice[J].Bulletin of Agricultural Science and Technology,2011(8):154-155.(in Chinese)
[6]MIZUMA T,FURUKAWA S,KIYOKAWA Y,et al.Characteristics of low-glutelin rice for sake brewing:studies on rice for sake brewing[J].Seibutsu-kogaku Kaishi,2002,80(11):503-511.
[7]FURUKAWA S,ITOU M,MASUMURA T,et al.Ultrastructure of low-glutelin rice endosperm[J].Plant Biotechnology,2007,24(2):227-229.
[8]耿春苗.氮肥及播期对低谷蛋白水稻产量和品质形成的影响[D].南京:南京农业大学,2011.GENG C M.Effect of nitrogen and seeding date on the yield and quality of low glutelin rice[D].Nanjing:Nanjing Agricultural University,2011.(in Chinese with English abstract)
[9]MOCHIZUKI T,HARA S.Usefulness of the low protein rice on the diet therapy in patients with chronic renal failure[J].Nihon Jinzo Gakkai Shi,2000,42(1):24-29.
[10]杨静,罗秋香,钱春荣,等.氮素对稻米蛋白质组分含量及蒸煮食味品质的影响[J].东北农业大学学报,2006,37(2):145-150.YANG J,LUO Q X,QIAN C R,et al.Effects of nitrogen on the protein fractions content and cooking and eating quality of rice[J].Journal of Northeast Agricultural University,2006,37(2):145-150.(in Chinese with English abstract)
[11]汤章城.现代植物生理学实验指南[M].北京:科学出版社,1999.
[12]陈留根,张岳芳,王子臣,等.施氮量对机插粳型超级稻主要米质性状及淀粉RVA谱特征的影响[J].中国农学通报,2010,26(16):139-143.CHEN L G,ZHANG Y F,WANG Z C,et al.Effects of nitrogen application rate on rice quality and starch RVA profile parameters of mechanical transplanting super japonica rice[J].Chinese Agricultural Science Bulletin,2010,26(16):139-143.(in Chinese with English abstract)
[13]金军,徐大勇,蔡一霞,等.施氮量对水稻主要米质性状及RVA谱特征参数的影响[J].作物学报,2004,30(2):154-158.JIN J,XU D Y,CAI Y X,et al.Effect of N-fertilizer on main quality characters of rice and RVA profile parameters[J].Acta Agronomica Sinica,2004,30(2):154-158.(in Chinese with English abstract)
[14]兰艳,黄鹏,江谷驰弘,等.成都平原稻作区施氮量和栽插密度对粳稻D46产量及品质的影响[J].浙江大学学报(农业与生命科学版),2016,42(1):63-73.LAN Y,HUANG P,JIANGGU C H,et al.Effect of nitrogen application and planting density on grain yield and quality of japonica rice cultivar D46 in the planting area of Chengdu plain[J].Journal of Zhejiang University(Agriculture&Life Sciences),2016,42(1):63-73.(in Chinese with English abstract)
[15]闫影,张丽霞,万常照,等.稻米淀粉RVA谱特征值及理化指标与食味值的相关性[J].植物生理学报,2016,52(12):1884-1890.YAN Y,ZHANG L X,WAN C Z,et al.Correlation between RVA spectral characteristic value and physical and chemical index of rice starch and taste value[J].Plant Physiology Journal,2016,52(12):1884-1890.(in Chinese with English abstract)
[16]张小明,石春海,富田桂.粳稻米淀粉特性与食味间的相关性分析[J].中国水稻科学,2002,16(2):157-161.ZHANG X M,SHI C H,FU T G.Correlation analysis between starch characteristics and taste quality in japonica rice[J].Chinese Journal Rice Science,2002,16(2):157-161.(in Chinese with English abstract)
[17]季红娟,赵步洪,陈刚,等.扬粳805产量和品质对麦秸还田与施氮量的响应[J].扬州大学学报(农业与生命科学版),2016,37(4):60-64.JI H J,ZHAO B H,CHEN G,et al.Response of grain yield and quality of Yangjing 805 to wheat straw returning and nitrogen management[J].Journal of Yangzhou University(Agriculture and Life Sciences),2016,37(4):60-64.(in Chinese with English abstract)
[18]王玉文,李会霞,田岗,等.小米外观品质及淀粉RVA谱特征与米饭适口性的关系[J].山西农业科学,2008,36(7):34-39.WANG Y W,LI H X,TIAN G,et al.Relationship between cooked millet palatability and both visual quality and RVAprofile character of starch[J].Shanxi Agricultural Sciences,2008,36(7):34-39.(in Chinese with English abstract)
[19]吴洪恺,刘世家,江玲,等.稻米蛋白质组分及总蛋白质含量与淀粉RVA谱特征值的关系[J].中国水稻科学,2009,23(4):421-426.WU H K,LIU S J,JIANG L,et al.Relationship between protein composition and total protein content and starch RVAprofile properties in rice[J].Chinese Journal Rice Science,2009,23(4):421-426.(in Chinese with English abstract)
[20]LI G H,CHEN Y L,DING Y F,et al.Charactering protein fraction concentrations as influenced by nitrogen application in low-glutelin rice cultivars[J].Journal of Integrative Agriculture,2016,15(3):537-544.
[21]魏海燕,王亚江,孟天瑶,等.机插超级粳稻产量、品质及氮肥利用率对氮肥的响应[J].应用生态学报,2014,25(2):488-496.WEI H Y,WANG Y J,MENG T Y,et al.Response of yield,quality and nitrogen use efficiency to nitrogen fertilizer from mechanical transplanting super japonica rice[J].Chinese Journal of Applied Ecology,2014,25(2):488-496.(in Chinese with English abstract)
[22]程效义,徐海,马作斌,等.施氮量与栽插密度对粳稻稻米品质的影响[J].杂交水稻,2011,26(5):77-80.CHENG X Y,XU H,MA Z B,et al.Effects of nitrogen rate and transplanting density on grain quality of japonica rice[J].Hybrid Rice,2011,26(5):77-80.(in Chinese with English abstract)
[23]从夕汉,施伏芝,阮新民,等.氮肥水平对不同基因型水稻氮素利用率、产量和品质的影响[J].应用生态学报,2017,28(4):1219-1226.CONG X H,SHI F Z,RUAN X M,et al.Effects of nitrogen fertilizer application rate on nitrogen use efficiency and grain yield and quality of different rice varieties[J].Chinese Journal of Applied Ecology,2017,28(4):1219-1226.(in Chinese with English abstract)
[24]池忠志,赵广才,常旭虹,等.施氮量对冬小麦籽粒蛋白质及其组分含量和比例的影响[J].麦类作物学报,2006,26(6):65-69.CHI Z Z,ZHAO G C,CHANG X H,et al.Effect of nitrogen application amount on protein and its components in grain of winter wheat[J].Journal of Triticeae Crops,2006,26(6):65-69.(in Chinese with English abstract)
[25]池忠志,赵广才,郑家国,等.施氮量对不同品种小麦籽粒蛋白组分和加工品质的影响[J].西南农业学报,2010,23(2):427-431.CHI Z Z,ZHAO G C,ZHENG J G,et al.Effect of nitrogen application amount on protein components in grain of different varieties wheat[J].Southwest China Journal of Agricultural Sciences,2010,23(2):427-431.(in Chinese with English abstract)
[26]王月福,于振文,李尚霞,等.施氮量对小麦籽粒蛋白质组分含量及加工品质的影响[J].中国农业科学,2002,35(9):1071-1078.WANG Y F,YU Z W,LI S X,et al.Effects of nitrogen application amount on content of protein components and processing quality of wheat grain[J].Scientia Agricultura Sinica,2002,35(9):1071-1078.(in Chinese with English abstract)
[27]王美,赵广才,石书兵,等.施氮量对不同粒色小麦花后光合特性及成熟期氮素分配和籽粒蛋白质组分的影响[J].麦类作物学报,2015,35(6):829-835.WANG M,ZHAO G C,SHI S B,et al.Effect of nitrogen rate on photos synthesis after anthesis,nitrogen distribution and content of protein and its component in maturity stage in wheat with different seed color[J].Journal of Triticeae Crops,2015,35(6):829-835.(in Chinese with English abstract)
[28]张欣,施利利,刘晓宇,等.不同施肥处理对水稻产量、食味品质及蛋白质组分的影响[J].中国农学通报,2010,26(4):104-108.ZHANG X,SHI L L,LIU X Y,et al.Effect of different fertilizer treatments on rice yield,grain quality and protein fraction content[J].Chinese Agricultural Science Bulletin,2010,26(4):104-108.(in Chinese with English abstract)
[29]王爽.氮肥用量和插秧密度对寒地粳稻蛋白质及稻米品质的影响[D].哈尔滨:东北农业大学,2015.WANG S.Effect of nitrogen application and transplanting density on protein content and rice quality in cold japonica rice[D].Harbin:Northeast Agricultural University,2015.(in Chinese with English abstract)
[30]陈依露.播期对低谷蛋白水稻产量及功能特性的影响[D].南京:南京农业大学,2014.CHEN Y L.Effects of seeding date on grain yield and functional characteristics of low glutelin rice[D].Nanjing:Nanjing Agricultural University,2014.(in Chinese with English abstract)
[31]王爽,赵宏伟,姚辰,等.氮肥用量和插秧密度对寒地粳稻籽粒转氨酶活性及蛋白质含量的影响[J].中国土壤与肥料,2015(2):70-77.WANG S,ZHAO H W,YAO C,et al.Effect of N fertilizer application and transplanting density on grain transaminase activity and protein content in japonica rice in cold region[J].China Soil and Fertilizer,2015(2):70-77.(in Chinese with English abstract)
[32]张自常,李鸿伟,曹转勤,等.施氮量和灌溉方式的交互作用对水稻产量和品质影响[J].作物学报,2013,39(1):82-84.ZHANG Z C,LI H W,CAO Z Q,et al.Effect of interaction between nitrogen rate and irrigation regime on grain yield and quality of rice[J].Acta Agronomica Sinica,2013,39(1):82-84.(in Chinese with English abstract)
[33]董作珍,吴良欢,柴婕,等.不同氮磷钾处理对中浙优1号水稻产量、品质、养分吸收利用及经济效益的影响[J].中国水稻科学,2015,29(4):399-407.DONG Z Z,WU L H,CHAI J,et al.Effects of different nitrogen,phosphorus and potassium treatments on rice yield,quality,nutrient absorption-utilization and economic benefit of Zhongzheyou1 in central Zhejiang province,China[J].Chinese Journal Rice Science,2015,29(4):399-407.(in Chinese with English abstract)
[34]徐大勇,方兆伟,高云,等.N肥对水稻籽粒蛋白质含量、组成成分和氨基酸含量影响的研究[C]//中国青年农业科学学术年报.北京:中国农业出版社,2004.
[35]赵宁春,张小明,叶胜海,等.不同栽培方式和施氮量对稻米营养品质及植酸积累的影响[J].浙江农业学报,2009,21(3):259-263.ZHAO N C,ZHANG X M,YE S H,et al.Effects of different cultivation methods and nitrogen application on grain phytic acid contents and nutritional quality for japonica rice[J].Acta Agriculturae Zhejiangensis,2009,21(3):259-263.(in Chinese with English abstract)
[2]刘海,赵欢,何佳芳,等.稻米营养品质影响因素研究进展[J].贵州农业科学,2013,41(6):85-89.LIU H,ZHAO H,HE G F,et al.Advances in the influencing factors of rice nutritional quality[J].Guizhou Agricultural Sciences,2013,41(6):85-89.(in Chinese with English abstract)
[3]周丽慧,刘巧泉,张昌泉,等.水稻种子蛋白质含量及组分在品种间的变异与分布[J].作物学报,2009,35(5):884-891.ZHOU L H,LIU Q Q,ZHANG C Q,et al.Variation and distribution of seed storage protein content and composition among different rice varieties[J].Acta Agronomica Sinica,2009,35(5):884-891.(in Chinese with English abstract)
[4]张莉萍,陈少龙,孟巧霞,等.水稻低谷蛋白研究进展[J].农业科技通讯,2012(5):7-8.ZHANG L P,CHEN S L,MENG Q X,et al.Advances in the rice low-gluten protein research[J].Bulletin of Agricultural Science and Technology,2012(5):7-8.(in Chinese)
[5]蔡金洋,杨尧城.低含量谷蛋白水稻研究进展[J].农业科技通讯,2011(8):154-155.CAI J Y,YANG Y C.Advance of the low content gluten rice[J].Bulletin of Agricultural Science and Technology,2011(8):154-155.(in Chinese)
[6]MIZUMA T,FURUKAWA S,KIYOKAWA Y,et al.Characteristics of low-glutelin rice for sake brewing:studies on rice for sake brewing[J].Seibutsu-kogaku Kaishi,2002,80(11):503-511.
[7]FURUKAWA S,ITOU M,MASUMURA T,et al.Ultrastructure of low-glutelin rice endosperm[J].Plant Biotechnology,2007,24(2):227-229.
[8]耿春苗.氮肥及播期对低谷蛋白水稻产量和品质形成的影响[D].南京:南京农业大学,2011.GENG C M.Effect of nitrogen and seeding date on the yield and quality of low glutelin rice[D].Nanjing:Nanjing Agricultural University,2011.(in Chinese with English abstract)
[9]MOCHIZUKI T,HARA S.Usefulness of the low protein rice on the diet therapy in patients with chronic renal failure[J].Nihon Jinzo Gakkai Shi,2000,42(1):24-29.
[10]杨静,罗秋香,钱春荣,等.氮素对稻米蛋白质组分含量及蒸煮食味品质的影响[J].东北农业大学学报,2006,37(2):145-150.YANG J,LUO Q X,QIAN C R,et al.Effects of nitrogen on the protein fractions content and cooking and eating quality of rice[J].Journal of Northeast Agricultural University,2006,37(2):145-150.(in Chinese with English abstract)
[11]汤章城.现代植物生理学实验指南[M].北京:科学出版社,1999.
[12]陈留根,张岳芳,王子臣,等.施氮量对机插粳型超级稻主要米质性状及淀粉RVA谱特征的影响[J].中国农学通报,2010,26(16):139-143.CHEN L G,ZHANG Y F,WANG Z C,et al.Effects of nitrogen application rate on rice quality and starch RVA profile parameters of mechanical transplanting super japonica rice[J].Chinese Agricultural Science Bulletin,2010,26(16):139-143.(in Chinese with English abstract)
[13]金军,徐大勇,蔡一霞,等.施氮量对水稻主要米质性状及RVA谱特征参数的影响[J].作物学报,2004,30(2):154-158.JIN J,XU D Y,CAI Y X,et al.Effect of N-fertilizer on main quality characters of rice and RVA profile parameters[J].Acta Agronomica Sinica,2004,30(2):154-158.(in Chinese with English abstract)
[14]兰艳,黄鹏,江谷驰弘,等.成都平原稻作区施氮量和栽插密度对粳稻D46产量及品质的影响[J].浙江大学学报(农业与生命科学版),2016,42(1):63-73.LAN Y,HUANG P,JIANGGU C H,et al.Effect of nitrogen application and planting density on grain yield and quality of japonica rice cultivar D46 in the planting area of Chengdu plain[J].Journal of Zhejiang University(Agriculture&Life Sciences),2016,42(1):63-73.(in Chinese with English abstract)
[15]闫影,张丽霞,万常照,等.稻米淀粉RVA谱特征值及理化指标与食味值的相关性[J].植物生理学报,2016,52(12):1884-1890.YAN Y,ZHANG L X,WAN C Z,et al.Correlation between RVA spectral characteristic value and physical and chemical index of rice starch and taste value[J].Plant Physiology Journal,2016,52(12):1884-1890.(in Chinese with English abstract)
[16]张小明,石春海,富田桂.粳稻米淀粉特性与食味间的相关性分析[J].中国水稻科学,2002,16(2):157-161.ZHANG X M,SHI C H,FU T G.Correlation analysis between starch characteristics and taste quality in japonica rice[J].Chinese Journal Rice Science,2002,16(2):157-161.(in Chinese with English abstract)
[17]季红娟,赵步洪,陈刚,等.扬粳805产量和品质对麦秸还田与施氮量的响应[J].扬州大学学报(农业与生命科学版),2016,37(4):60-64.JI H J,ZHAO B H,CHEN G,et al.Response of grain yield and quality of Yangjing 805 to wheat straw returning and nitrogen management[J].Journal of Yangzhou University(Agriculture and Life Sciences),2016,37(4):60-64.(in Chinese with English abstract)
[18]王玉文,李会霞,田岗,等.小米外观品质及淀粉RVA谱特征与米饭适口性的关系[J].山西农业科学,2008,36(7):34-39.WANG Y W,LI H X,TIAN G,et al.Relationship between cooked millet palatability and both visual quality and RVAprofile character of starch[J].Shanxi Agricultural Sciences,2008,36(7):34-39.(in Chinese with English abstract)
[19]吴洪恺,刘世家,江玲,等.稻米蛋白质组分及总蛋白质含量与淀粉RVA谱特征值的关系[J].中国水稻科学,2009,23(4):421-426.WU H K,LIU S J,JIANG L,et al.Relationship between protein composition and total protein content and starch RVAprofile properties in rice[J].Chinese Journal Rice Science,2009,23(4):421-426.(in Chinese with English abstract)
[20]LI G H,CHEN Y L,DING Y F,et al.Charactering protein fraction concentrations as influenced by nitrogen application in low-glutelin rice cultivars[J].Journal of Integrative Agriculture,2016,15(3):537-544.
[21]魏海燕,王亚江,孟天瑶,等.机插超级粳稻产量、品质及氮肥利用率对氮肥的响应[J].应用生态学报,2014,25(2):488-496.WEI H Y,WANG Y J,MENG T Y,et al.Response of yield,quality and nitrogen use efficiency to nitrogen fertilizer from mechanical transplanting super japonica rice[J].Chinese Journal of Applied Ecology,2014,25(2):488-496.(in Chinese with English abstract)
[22]程效义,徐海,马作斌,等.施氮量与栽插密度对粳稻稻米品质的影响[J].杂交水稻,2011,26(5):77-80.CHENG X Y,XU H,MA Z B,et al.Effects of nitrogen rate and transplanting density on grain quality of japonica rice[J].Hybrid Rice,2011,26(5):77-80.(in Chinese with English abstract)
[23]从夕汉,施伏芝,阮新民,等.氮肥水平对不同基因型水稻氮素利用率、产量和品质的影响[J].应用生态学报,2017,28(4):1219-1226.CONG X H,SHI F Z,RUAN X M,et al.Effects of nitrogen fertilizer application rate on nitrogen use efficiency and grain yield and quality of different rice varieties[J].Chinese Journal of Applied Ecology,2017,28(4):1219-1226.(in Chinese with English abstract)
[24]池忠志,赵广才,常旭虹,等.施氮量对冬小麦籽粒蛋白质及其组分含量和比例的影响[J].麦类作物学报,2006,26(6):65-69.CHI Z Z,ZHAO G C,CHANG X H,et al.Effect of nitrogen application amount on protein and its components in grain of winter wheat[J].Journal of Triticeae Crops,2006,26(6):65-69.(in Chinese with English abstract)
[25]池忠志,赵广才,郑家国,等.施氮量对不同品种小麦籽粒蛋白组分和加工品质的影响[J].西南农业学报,2010,23(2):427-431.CHI Z Z,ZHAO G C,ZHENG J G,et al.Effect of nitrogen application amount on protein components in grain of different varieties wheat[J].Southwest China Journal of Agricultural Sciences,2010,23(2):427-431.(in Chinese with English abstract)
[26]王月福,于振文,李尚霞,等.施氮量对小麦籽粒蛋白质组分含量及加工品质的影响[J].中国农业科学,2002,35(9):1071-1078.WANG Y F,YU Z W,LI S X,et al.Effects of nitrogen application amount on content of protein components and processing quality of wheat grain[J].Scientia Agricultura Sinica,2002,35(9):1071-1078.(in Chinese with English abstract)
[27]王美,赵广才,石书兵,等.施氮量对不同粒色小麦花后光合特性及成熟期氮素分配和籽粒蛋白质组分的影响[J].麦类作物学报,2015,35(6):829-835.WANG M,ZHAO G C,SHI S B,et al.Effect of nitrogen rate on photos synthesis after anthesis,nitrogen distribution and content of protein and its component in maturity stage in wheat with different seed color[J].Journal of Triticeae Crops,2015,35(6):829-835.(in Chinese with English abstract)
[28]张欣,施利利,刘晓宇,等.不同施肥处理对水稻产量、食味品质及蛋白质组分的影响[J].中国农学通报,2010,26(4):104-108.ZHANG X,SHI L L,LIU X Y,et al.Effect of different fertilizer treatments on rice yield,grain quality and protein fraction content[J].Chinese Agricultural Science Bulletin,2010,26(4):104-108.(in Chinese with English abstract)
[29]王爽.氮肥用量和插秧密度对寒地粳稻蛋白质及稻米品质的影响[D].哈尔滨:东北农业大学,2015.WANG S.Effect of nitrogen application and transplanting density on protein content and rice quality in cold japonica rice[D].Harbin:Northeast Agricultural University,2015.(in Chinese with English abstract)
[30]陈依露.播期对低谷蛋白水稻产量及功能特性的影响[D].南京:南京农业大学,2014.CHEN Y L.Effects of seeding date on grain yield and functional characteristics of low glutelin rice[D].Nanjing:Nanjing Agricultural University,2014.(in Chinese with English abstract)
[31]王爽,赵宏伟,姚辰,等.氮肥用量和插秧密度对寒地粳稻籽粒转氨酶活性及蛋白质含量的影响[J].中国土壤与肥料,2015(2):70-77.WANG S,ZHAO H W,YAO C,et al.Effect of N fertilizer application and transplanting density on grain transaminase activity and protein content in japonica rice in cold region[J].China Soil and Fertilizer,2015(2):70-77.(in Chinese with English abstract)
[32]张自常,李鸿伟,曹转勤,等.施氮量和灌溉方式的交互作用对水稻产量和品质影响[J].作物学报,2013,39(1):82-84.ZHANG Z C,LI H W,CAO Z Q,et al.Effect of interaction between nitrogen rate and irrigation regime on grain yield and quality of rice[J].Acta Agronomica Sinica,2013,39(1):82-84.(in Chinese with English abstract)
[33]董作珍,吴良欢,柴婕,等.不同氮磷钾处理对中浙优1号水稻产量、品质、养分吸收利用及经济效益的影响[J].中国水稻科学,2015,29(4):399-407.DONG Z Z,WU L H,CHAI J,et al.Effects of different nitrogen,phosphorus and potassium treatments on rice yield,quality,nutrient absorption-utilization and economic benefit of Zhongzheyou1 in central Zhejiang province,China[J].Chinese Journal Rice Science,2015,29(4):399-407.(in Chinese with English abstract)
[34]徐大勇,方兆伟,高云,等.N肥对水稻籽粒蛋白质含量、组成成分和氨基酸含量影响的研究[C]//中国青年农业科学学术年报.北京:中国农业出版社,2004.
[35]赵宁春,张小明,叶胜海,等.不同栽培方式和施氮量对稻米营养品质及植酸积累的影响[J].浙江农业学报,2009,21(3):259-263.ZHAO N C,ZHANG X M,YE S H,et al.Effects of different cultivation methods and nitrogen application on grain phytic acid contents and nutritional quality for japonica rice[J].Acta Agriculturae Zhejiangensis,2009,21(3):259-263.(in Chinese with English abstract)