8-羟基喹啉微乳液光度法快速测定药材中总铝和活性铝
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摘要
建立新的测定铝的8-羟基喹啉微乳液光度法。用8-羟基喹啉与样品提取液中的铝反应,然后用微乳液萃取,在376 nm波长处测定吸光度。在选取的适宜条件下,Al~(3+)含量在0"25μg/8 mL(8 mL是微乳液体积)内符合比尔定律,线性相关系数R2=0. 9986。重复性实验的RSD分别是活性铝为2. 4%,而总铝为3. 3%;加标回收率分别是活性铝为88. 7%～95. 9%,而总铝为96. 5%～105. 8%。本法准确可靠,适于试样中总铝和活性铝的快速分析。
A new determination of Al by 8-hydroxyquinoline microemulsion spectrophotometry was established. Al in the sample extract was reacted with 8-hydroxyquinoline,then microemulsion extraction was used to determine the absorbance at 376 nm wavelength. Under the optimum reaction condition,Beer's law was kept in the range of 0 "25 μg/8 mL( 8 mL was the adding microemulsion volume). The linear correlation coefficient R2 was 0. 9986. RSD of the repeativity test was 2. 4% for active Al,and 3. 3% for total Al. The recoveries were from 88. 7% to 95. 9% for active Al,and from 96. 5% to 105. 8% for total Al. The method was accurate and reliable and was suitable for rapid analysis of total aluminum and active aluminum in samples.
A new determination of Al by 8-hydroxyquinoline microemulsion spectrophotometry was established. Al in the sample extract was reacted with 8-hydroxyquinoline,then microemulsion extraction was used to determine the absorbance at 376 nm wavelength. Under the optimum reaction condition,Beer's law was kept in the range of 0 "25 μg/8 mL( 8 mL was the adding microemulsion volume). The linear correlation coefficient R2 was 0. 9986. RSD of the repeativity test was 2. 4% for active Al,and 3. 3% for total Al. The recoveries were from 88. 7% to 95. 9% for active Al,and from 96. 5% to 105. 8% for total Al. The method was accurate and reliable and was suitable for rapid analysis of total aluminum and active aluminum in samples.
引文
[1]干宁,毕树平,邹公伟.氯仿萃取8-羟基喹啉荧光法测定天然水中的总单核铝和酸溶态铝[J].分析化学,2000,28(4):461-465.
[2]干宁,毕树平,邹公伟,等.离子交换/8-羟基喹啉荧光法分别测定天然水中的无机和有机单核铝[J].分析化学,2000,28(11):1375-1379.
[3]胡文宁,胡斌,郑菲. 8-羟基喹啉动态有序介孔Si O2涂层毛细管微萃取与ICP-MS在线联用分析天然水中铝形态[J].分析科学学报,2009,25(6):621-624.
[4]马莎,杨晓梅,赵荣华.明矾炮制前后半夏中铝形态分析[J].光谱实验室,2011,28(6):2983-2986.
[5]余萍,高俊杰,张东.微乳液析相液-液萃取分光光度法测定铝[J].理化检验-化学分册,2005,41(5):341-346.
[6]马莎,杨晓梅,薛咏梅,等.胃肠模拟系统对明矾炮制药材中铝的形态转变影响[J].光谱实验室,2012,29(5):3103-3105.
[2]干宁,毕树平,邹公伟,等.离子交换/8-羟基喹啉荧光法分别测定天然水中的无机和有机单核铝[J].分析化学,2000,28(11):1375-1379.
[3]胡文宁,胡斌,郑菲. 8-羟基喹啉动态有序介孔Si O2涂层毛细管微萃取与ICP-MS在线联用分析天然水中铝形态[J].分析科学学报,2009,25(6):621-624.
[4]马莎,杨晓梅,赵荣华.明矾炮制前后半夏中铝形态分析[J].光谱实验室,2011,28(6):2983-2986.
[5]余萍,高俊杰,张东.微乳液析相液-液萃取分光光度法测定铝[J].理化检验-化学分册,2005,41(5):341-346.
[6]马莎,杨晓梅,薛咏梅,等.胃肠模拟系统对明矾炮制药材中铝的形态转变影响[J].光谱实验室,2012,29(5):3103-3105.