乳酸菌活的非可培养态的研究进展
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摘要
乳酸菌在某些恶劣的环境下仍可以保持代谢活性,会失去在常规培养基上生长繁殖的能力从而进入活的非可培养态(Viable but non-culturable,VBNC)。文中阐述了目前已经发现的VBNC态乳酸菌的种类、其诱导和复苏条件、生理生化特性、检测方法及前景展望,使我们更深入地认识和了解乳酸菌的VBNC态,为今后乳酸菌在工业生产和应用过程中保持高活性状态提供新思路和新方法。
Lactic acid bacteria(LAB) respond to various environmental stresses by entry into a novel physiological state, where the cells remain viable but are no longer culturable on standard laboratory media, called‘Viable but non-culturable state'(VBNC).This paper summarizes the LAB species which has been found existence the VBNC state, also reviewed the condition of induction and recovery, physiological-biological characteristics, detection and resuscitation method as well as its prospect.All above could make us deeply knowledge and understand the VBNC state of LAB, which can provide new ideas and methods for the LAB which remands high-activity in industrial production and application in the future.
Lactic acid bacteria(LAB) respond to various environmental stresses by entry into a novel physiological state, where the cells remain viable but are no longer culturable on standard laboratory media, called‘Viable but non-culturable state'(VBNC).This paper summarizes the LAB species which has been found existence the VBNC state, also reviewed the condition of induction and recovery, physiological-biological characteristics, detection and resuscitation method as well as its prospect.All above could make us deeply knowledge and understand the VBNC state of LAB, which can provide new ideas and methods for the LAB which remands high-activity in industrial production and application in the future.
引文
[1]XU H S,ROBERTS N,SINGLETON F L,et al.Survival and viability of nonculturable Escherichia coli and Vibriocholerae in the estuarine and marine environment[J].Microbial Ecol,1982,8(4):313-323.
[2]PINTO D,SANTOS M A,CHAMBEL L.Thirty years of viable but nonculturable state research:unsolved molecular mechanism[J].Crit Rev.Microbiol,2013:1549-7828.
[3]张刚.乳酸细菌—基础、技术和应用[M].北京:化学工业出版社,2007
[4]郭兴华.益生乳酸细菌—分子生物学及生物技术[M].北京:科学出版社,2008
[5]TREVORS J T.Viable but non-culturable(VBNC)bacteria:Gene expression in planktonic and biofilm cells[J].Journal of Microbiological Methods,2011,86(2):266-273.
[6]李影.大肠杆菌、乳酸杆菌“活的非可培养状态”研究[D].吉林农业大学,2007.
[7]魏忠彬.乳酸杆菌活的非可培养状态的研究[D].吉林农业大学,2007.
[8]金磊,王丽,钟青萍,等.植物乳杆菌活的非可培养状态的初步研究[J].食品工业科技,2013,34(16):187-190.
[9]李影.大肠杆菌、乳酸杆菌“活的非可培养状态”研究[D].吉林农业大学,2007.
[10]许婷.双歧杆菌活的非可培养状态的研究[D].吉林农业大学,2008.
[11]张艳国,田雷.乳酸菌的耐酸性机制[J].饲料工业,2007,04:62-64.
[12]HEIM S,LLEO M D M,BONATO B,et al.The viable but nonculturable state and starvation are different stress responses of Enterococcus faecalis,as determined by proteome analysis[J].Journal of bacteriology,2002,184(23):6739-6745.
[13]GANESAN B,STUART M R,WEIMER B C,et al.Carbohydrate starvation causes a metabolically active but nonculturable state in Lactococcus lactis[J].Appl Environ Microbiol,2007,73(8):2498-512.
[14]NYSTROM T,LARSSON C,GUSTAFSSON L.Bacterial defense against aging:role of the Escherichia coli Arc A regulator in gene expression,readjusted energy flux and survival during stasis[J].The EMBO journal,1996,15(13):32-19.
[15]WONG H C,WANG P.Induction of Viable but Nonculturable State in Vibrio parahaemolyticus and Its Susceptibility to Environmental Stresses[J].Journal of Applied Microbiology,2004,96(2):359-366.
[16]DEL MAR LLEO M,TAFI M C,CANEPARI P,et al.Nonculturable Enterococcus faecalis cells are metabolically active and capable of resuming active growth[J].Systematic and applied microbiology,1998,21(3):333-339.
[17]PFEFFER J M,STRATING H,WEADGE J T,et al.Peptidoglycan Oacetylation and autolysin profile of Enterococcus faecalis in the viable but nonculturable state[J].Journal of bacteriology,2006,188(3):902-908.
[18]SIGNORETTO C,DEL MAR LLE?M,TAFI M C,et al.Cell Wall Chemical Composition of Enterococcus faecalis in the Viable but Nonculturable State[J].Applied and environmental microbiology,2000,66(5):1953-1959.
[19]邸聪聪,胡平,胡章立,等.细菌活的非可培养(VBNC)状态及其机理研究进展[J].应用与环境生物学报,2014,06:1124-1131.
[20]SIGNORETTO C,BURLACCHINI G,PRUZZO C,et al.Persistence of Enterococcus faecalis in aquatic environments via surface interactions with copepods[J].Applied and environmental microbiology,2005,71(5):2756-2761.
[21]RAMAMURTHY T,GHOSH A,PAZHANI G P,et al.Current perspectives on viable but non-culturable(VBNC)pathogenic bacteria[J].Frontiers in public health,2014,2.
[22]XIE Y,CHOU L,CUTLER A,et al.DNA macroarray profiling of Lactococcus lactis subsp.lactis IL1403 gene expression during environmental stresses[J].Applied and environmental microbiology,2004,70(11):6738-6747.
[23]LAHTINEN S J,AHOKOSKI H,REINIKAINEN J P,et al.Degradation of 16S r RNA and attributes of viability of viable but nonculturable probiotic bacteria[J].Letters in applied microbiology,2008,46(6):693-698.
[24]JOSEPHSON K L,GERBA C P,PEPPER I L,et al.Polymerase chain reaction detection of nonviable bacterial pathogens[J].Applied and Environmental Microbiology,1993,59(10):3513-3515.
[25]BEJ A K,MAHBUBANI M H,ATLAS R M,et al.Detection of viable Legionella pneumophila in water by polymerase chain reaction and gene probe methods[J].Applied and Environmental Microbiology,1991,57(2):597-600.
[26]DEL MAR LLEO M,TAFI M C,CANEPARI P,et al.Nonculturable Enterococcus faecalis cells are metabolically active and capable of resuming active growth[J].Systematic and applied microbiology,1998,21(3):333-339.
[27]DEL MAR LLE?M,PIEROBON S,TAFI M C,et al.m RNA detection by reverse transcription-PCR for monitoring viability over time in an Enterococcus faecalis viable but nonculturable population maintained in a laboratory microcosm[J].Applied and Environmental Microbiology,2000,66(10):4564-4567.
[28]KOGURE K,SIMIDU U,TAGA N,et al.A tentative direct microscopic method for counting living marine bacteria[J].Canadian Journal of Microbiology,1979,25(3):415-420.
[29]胡烨,赵瑞利,马吉飞,等.细菌活的但非可培养(VBNC)状态研究进展[J].科学时代,2013,(13).
[30]孙丰蓉.哈维氏弧菌活的非可培养状态的研究[D].中国海洋大学,2008.
[31]李影,魏忠彬,钱爱东,等.乳酸杆菌活的非可培养状态诱导条件的筛选与荧光观察[J].中国兽医科学,2008,38(10):878-883.
[32]陈慧婷,陶益,朱佳,等.藻细胞计数及死/活分析的流式细胞仪方法[J].深圳职业技术学院学报,2013,12(1):23-27.
[33]李影,王伟利,段冶,等.流式细胞仪检测活的非可培养状态大肠杆菌[J].中国动物检疫,2009,26(12):34-36.
[34]DAVIS C.Enumeration of probiotic strains:review of culture-dependent and alternative techniques to quantify viable bacteria[J].Journal of microbiological methods,2014,103:9-17.
[35]DU M,CHEN J,ZHANG X,et al.Retention of virulence in a viable but nonculturable Edwardsiella tarda isolate[J].Applied and environmental microbiology,2007,73(4):1349-1354.
[36]VATTAKAVEN T,BOND P,BRADLEY G,et al.Differential effects of temperature and starvation on induction of the viable-but-nonculturable state in the coral pathogens Vibrio shiloi and Vibrio tasmaniensis[J].Applied and environmental microbiology,2006,72(10):6508-6513.
[37]田聪,余以刚,肖性龙,等.活的非可培养状态大肠杆菌O157:H7的复苏研究[J].食品科学,2013,34(11):218-221.
[38]魏忠彬,李影,钱爱东,等.VBNC状态乳酸杆菌的复苏[J].中国饲料,2006(18):27-28.
[2]PINTO D,SANTOS M A,CHAMBEL L.Thirty years of viable but nonculturable state research:unsolved molecular mechanism[J].Crit Rev.Microbiol,2013:1549-7828.
[3]张刚.乳酸细菌—基础、技术和应用[M].北京:化学工业出版社,2007
[4]郭兴华.益生乳酸细菌—分子生物学及生物技术[M].北京:科学出版社,2008
[5]TREVORS J T.Viable but non-culturable(VBNC)bacteria:Gene expression in planktonic and biofilm cells[J].Journal of Microbiological Methods,2011,86(2):266-273.
[6]李影.大肠杆菌、乳酸杆菌“活的非可培养状态”研究[D].吉林农业大学,2007.
[7]魏忠彬.乳酸杆菌活的非可培养状态的研究[D].吉林农业大学,2007.
[8]金磊,王丽,钟青萍,等.植物乳杆菌活的非可培养状态的初步研究[J].食品工业科技,2013,34(16):187-190.
[9]李影.大肠杆菌、乳酸杆菌“活的非可培养状态”研究[D].吉林农业大学,2007.
[10]许婷.双歧杆菌活的非可培养状态的研究[D].吉林农业大学,2008.
[11]张艳国,田雷.乳酸菌的耐酸性机制[J].饲料工业,2007,04:62-64.
[12]HEIM S,LLEO M D M,BONATO B,et al.The viable but nonculturable state and starvation are different stress responses of Enterococcus faecalis,as determined by proteome analysis[J].Journal of bacteriology,2002,184(23):6739-6745.
[13]GANESAN B,STUART M R,WEIMER B C,et al.Carbohydrate starvation causes a metabolically active but nonculturable state in Lactococcus lactis[J].Appl Environ Microbiol,2007,73(8):2498-512.
[14]NYSTROM T,LARSSON C,GUSTAFSSON L.Bacterial defense against aging:role of the Escherichia coli Arc A regulator in gene expression,readjusted energy flux and survival during stasis[J].The EMBO journal,1996,15(13):32-19.
[15]WONG H C,WANG P.Induction of Viable but Nonculturable State in Vibrio parahaemolyticus and Its Susceptibility to Environmental Stresses[J].Journal of Applied Microbiology,2004,96(2):359-366.
[16]DEL MAR LLEO M,TAFI M C,CANEPARI P,et al.Nonculturable Enterococcus faecalis cells are metabolically active and capable of resuming active growth[J].Systematic and applied microbiology,1998,21(3):333-339.
[17]PFEFFER J M,STRATING H,WEADGE J T,et al.Peptidoglycan Oacetylation and autolysin profile of Enterococcus faecalis in the viable but nonculturable state[J].Journal of bacteriology,2006,188(3):902-908.
[18]SIGNORETTO C,DEL MAR LLE?M,TAFI M C,et al.Cell Wall Chemical Composition of Enterococcus faecalis in the Viable but Nonculturable State[J].Applied and environmental microbiology,2000,66(5):1953-1959.
[19]邸聪聪,胡平,胡章立,等.细菌活的非可培养(VBNC)状态及其机理研究进展[J].应用与环境生物学报,2014,06:1124-1131.
[20]SIGNORETTO C,BURLACCHINI G,PRUZZO C,et al.Persistence of Enterococcus faecalis in aquatic environments via surface interactions with copepods[J].Applied and environmental microbiology,2005,71(5):2756-2761.
[21]RAMAMURTHY T,GHOSH A,PAZHANI G P,et al.Current perspectives on viable but non-culturable(VBNC)pathogenic bacteria[J].Frontiers in public health,2014,2.
[22]XIE Y,CHOU L,CUTLER A,et al.DNA macroarray profiling of Lactococcus lactis subsp.lactis IL1403 gene expression during environmental stresses[J].Applied and environmental microbiology,2004,70(11):6738-6747.
[23]LAHTINEN S J,AHOKOSKI H,REINIKAINEN J P,et al.Degradation of 16S r RNA and attributes of viability of viable but nonculturable probiotic bacteria[J].Letters in applied microbiology,2008,46(6):693-698.
[24]JOSEPHSON K L,GERBA C P,PEPPER I L,et al.Polymerase chain reaction detection of nonviable bacterial pathogens[J].Applied and Environmental Microbiology,1993,59(10):3513-3515.
[25]BEJ A K,MAHBUBANI M H,ATLAS R M,et al.Detection of viable Legionella pneumophila in water by polymerase chain reaction and gene probe methods[J].Applied and Environmental Microbiology,1991,57(2):597-600.
[26]DEL MAR LLEO M,TAFI M C,CANEPARI P,et al.Nonculturable Enterococcus faecalis cells are metabolically active and capable of resuming active growth[J].Systematic and applied microbiology,1998,21(3):333-339.
[27]DEL MAR LLE?M,PIEROBON S,TAFI M C,et al.m RNA detection by reverse transcription-PCR for monitoring viability over time in an Enterococcus faecalis viable but nonculturable population maintained in a laboratory microcosm[J].Applied and Environmental Microbiology,2000,66(10):4564-4567.
[28]KOGURE K,SIMIDU U,TAGA N,et al.A tentative direct microscopic method for counting living marine bacteria[J].Canadian Journal of Microbiology,1979,25(3):415-420.
[29]胡烨,赵瑞利,马吉飞,等.细菌活的但非可培养(VBNC)状态研究进展[J].科学时代,2013,(13).
[30]孙丰蓉.哈维氏弧菌活的非可培养状态的研究[D].中国海洋大学,2008.
[31]李影,魏忠彬,钱爱东,等.乳酸杆菌活的非可培养状态诱导条件的筛选与荧光观察[J].中国兽医科学,2008,38(10):878-883.
[32]陈慧婷,陶益,朱佳,等.藻细胞计数及死/活分析的流式细胞仪方法[J].深圳职业技术学院学报,2013,12(1):23-27.
[33]李影,王伟利,段冶,等.流式细胞仪检测活的非可培养状态大肠杆菌[J].中国动物检疫,2009,26(12):34-36.
[34]DAVIS C.Enumeration of probiotic strains:review of culture-dependent and alternative techniques to quantify viable bacteria[J].Journal of microbiological methods,2014,103:9-17.
[35]DU M,CHEN J,ZHANG X,et al.Retention of virulence in a viable but nonculturable Edwardsiella tarda isolate[J].Applied and environmental microbiology,2007,73(4):1349-1354.
[36]VATTAKAVEN T,BOND P,BRADLEY G,et al.Differential effects of temperature and starvation on induction of the viable-but-nonculturable state in the coral pathogens Vibrio shiloi and Vibrio tasmaniensis[J].Applied and environmental microbiology,2006,72(10):6508-6513.
[37]田聪,余以刚,肖性龙,等.活的非可培养状态大肠杆菌O157:H7的复苏研究[J].食品科学,2013,34(11):218-221.
[38]魏忠彬,李影,钱爱东,等.VBNC状态乳酸杆菌的复苏[J].中国饲料,2006(18):27-28.