2015-2017年阿坝州活禽场所禽流感病毒污染状况调查
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摘要
目的了解阿坝州监测场所禽流感病毒污染、分类、组成和分布特点,为禽流感的防控提供指导。方法采集阿坝州禽类监测场所外环境标本,采用荧光定量PCR技术对禽流感A型通用及H5、H7、H9亚型检测,并进行统计学分析。结果共采集1 280份标本,A型通用及H5、H7、H9亚型的阳性率分别为10. 16%、0. 70%、3. 20%和6. 25%。2016-12 H9亚型阳性率最高,2017-04 H5、H7亚型阳性率最高;城乡活禽市场和家禽规模家禽养殖场检出A型流感,阳性率分别为14. 55%和5. 60%;禽类饮水流感病毒阳性率最高(18. 01%),其次为清洗禽类的污水(17. 24%)。结论阿坝州禽流感病毒检出率较高,存在人感染风险,应加强禽市场监管。
Objective To understand the pollution,composition and distribution characteristics of avian influenza virus in live poultry related environments in Aba and provide evidence for avian influenza prevention and control. Methods The external environment samples were collected from live poultry related environments for the detection of influenza A virus including subtype H5,H7 and H9 by fluorescent quantitative RT-PCR,and the results were statistically analyzed. Results A total of 1 280 specimens were collected. The positive rates of type A,H5,H7 and H9 influenza viruses were 10. 16%,0. 70%,3. 20% and 6. 25%,respectively. The H9 positive rate was the highest in December 2016. H5 and H7 positive rates were the highest in April 2017.Influenza A virus was detected in both urban and rural live poultry markets and poultry scale farms,with type A influenza virus positive rate being 14. 55% and 5. 60% respectively. Among different samples,poultry drinking water showed the highest positive rate( 18. 01%),followed by sewage( 17. 24%). Conclusion The positive rate of influenza virus is relatively high,and there is a potential risk of human infection. Therefore,it is critical to enhance the market supervision.
Objective To understand the pollution,composition and distribution characteristics of avian influenza virus in live poultry related environments in Aba and provide evidence for avian influenza prevention and control. Methods The external environment samples were collected from live poultry related environments for the detection of influenza A virus including subtype H5,H7 and H9 by fluorescent quantitative RT-PCR,and the results were statistically analyzed. Results A total of 1 280 specimens were collected. The positive rates of type A,H5,H7 and H9 influenza viruses were 10. 16%,0. 70%,3. 20% and 6. 25%,respectively. The H9 positive rate was the highest in December 2016. H5 and H7 positive rates were the highest in April 2017.Influenza A virus was detected in both urban and rural live poultry markets and poultry scale farms,with type A influenza virus positive rate being 14. 55% and 5. 60% respectively. Among different samples,poultry drinking water showed the highest positive rate( 18. 01%),followed by sewage( 17. 24%). Conclusion The positive rate of influenza virus is relatively high,and there is a potential risk of human infection. Therefore,it is critical to enhance the market supervision.
引文
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[8]龙术国,张金艳,熊新平. 2014-2015年株洲市禽类职业暴露人群及外环境禽流感病毒监测分析[J].实用预防医学,2016,23(7):854-856.
[9]刘红莲,郑惠能,颜玉炳,等. 2011年-2014年厦门市环境和职业暴露人群禽流感监测分析[J].中国卫生检验杂志,2016,26(1):109-112.
[10]张兵,朱军礼,朱淑英.金华市禽类职业暴露人群及外环境禽流感病毒监测分析[J].中国卫生检验杂志,2017,27(2):235-237.
[11]任瑞琦,周蕾,向妮娟,等.中国内地人感染H7N9禽流感疫情流行病学特征分析[J].中华流行病学杂志,2014,35(12):1362-1365.
[2]潘英姿,赵学成,袁春燕,等. 2013-2015年常州市武进区禽流感职业暴露人群及环境监测分析[J].现代预防医学,2016,43(8):1384-1387.
[3]闫铁成,肖丹,王波,等.中国大陆130例人感染H7N9禽流感病例流行病学特征分析[J].中华疾病控制杂志,2013,17(8):651-654.
[4]中国疾病预防控制中心.职业暴露人群血清学和环境高致病性禽流感监测方案(2011版)[S].北京:中国疾病预防控制中心,2011.
[5]朱闻斐,舒跃龙.新型H7N9禽流感病毒的病原学研究进展[J].生命科学,2015,27(5):518-524.
[6]刘静雯,刘慧,陆剑云,等.广州市2013-2015年人感染H7N9禽流感外环境监测结果分析[J].中国公共卫生,2016,32(10):1382-1386.
[7]孙宝昌,吴矛矛,朱传新,等. 2011-2016年浙江省温州地区活禽场所禽流感病毒监测分析[J].疾病监测,2017,32(4):328-331.
[8]龙术国,张金艳,熊新平. 2014-2015年株洲市禽类职业暴露人群及外环境禽流感病毒监测分析[J].实用预防医学,2016,23(7):854-856.
[9]刘红莲,郑惠能,颜玉炳,等. 2011年-2014年厦门市环境和职业暴露人群禽流感监测分析[J].中国卫生检验杂志,2016,26(1):109-112.
[10]张兵,朱军礼,朱淑英.金华市禽类职业暴露人群及外环境禽流感病毒监测分析[J].中国卫生检验杂志,2017,27(2):235-237.
[11]任瑞琦,周蕾,向妮娟,等.中国内地人感染H7N9禽流感疫情流行病学特征分析[J].中华流行病学杂志,2014,35(12):1362-1365.