羟丙基-β-环糊精手性流动相添加剂法拆分盐酸氯丙那林
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摘要
建立了以羟丙基-β-环糊精(HP-β-CD)为手性流动相添加剂、拆分盐酸氯丙那林的方法。使用C18色谱柱,考察了有机修饰剂的种类及配比、流动相的流速对拆分的影响。结果表明,当流动相为乙腈-甲醇-水(体积比为80∶5∶15),HP-β-CD浓度为0.8mmol·L~(-1),流速为0.8m L·min~(-1),检测波长为216nm时,盐酸氯丙那林的分离度为1.45。
The enantiomeric separation of clorprenaline hydrochloride was studied using the hydroxypropyl-β-cyclodextrin(HP-β-CD) as chiral mobile phase additive. The influences of type and concentration of organic modifier, the flow rate on the enantiomers separation were investigated on a C18 column. when The results showed that the mobile phase consist of acetonitrile-methanol-water was 80:5:15(V/V/V), contained 0.8 mmol/L HP-β-CD, at the flow rate of 0.8 m L/min, the detection wavelength was at 216 nm, the resolution of clorprenaline hydrochloride enantiomers was 1.45.
The enantiomeric separation of clorprenaline hydrochloride was studied using the hydroxypropyl-β-cyclodextrin(HP-β-CD) as chiral mobile phase additive. The influences of type and concentration of organic modifier, the flow rate on the enantiomers separation were investigated on a C18 column. when The results showed that the mobile phase consist of acetonitrile-methanol-water was 80:5:15(V/V/V), contained 0.8 mmol/L HP-β-CD, at the flow rate of 0.8 m L/min, the detection wavelength was at 216 nm, the resolution of clorprenaline hydrochloride enantiomers was 1.45.
引文
[1]卓开华,周征.HPLC法测定盐酸氯丙那林片的含量和含量均匀度[J].药物分析杂志,2007,27(4):588-589.
[2]彭志兵.PLC法同时测定海珠喘息定片中盐酸去氯羟嗪和盐酸氯丙那林的含量[J].宜春学院学报,2008,30(2):85-87.
[3]Xueqin Ren,Yiyang Dong,Aijin Huang.Separation of the enantiomers of four chiral drugs by neutral cyclodextrinmediated capillary zone electrophoresis[J].Chromatographia,1999,49(7/8):411-414.
[4]王立艳,苏立强,张磊.手性流动相添加剂法拆分盐酸氯丙那林对映体[J].化学研究与应用,2010,22(8):1050-1053.
[5]Xiaoping Wang,Mengxia Lu,Zhisi Bu.Enantioseparation of 2-(substituted phenyl)propanoic acids with hydroxypropyl-β-cyclodextrin as a chiral additive:investigation of substituent influence on enantiorecognition[J].Chinese Journal of Chromatography,2017,435(5):544-550.
[2]彭志兵.PLC法同时测定海珠喘息定片中盐酸去氯羟嗪和盐酸氯丙那林的含量[J].宜春学院学报,2008,30(2):85-87.
[3]Xueqin Ren,Yiyang Dong,Aijin Huang.Separation of the enantiomers of four chiral drugs by neutral cyclodextrinmediated capillary zone electrophoresis[J].Chromatographia,1999,49(7/8):411-414.
[4]王立艳,苏立强,张磊.手性流动相添加剂法拆分盐酸氯丙那林对映体[J].化学研究与应用,2010,22(8):1050-1053.
[5]Xiaoping Wang,Mengxia Lu,Zhisi Bu.Enantioseparation of 2-(substituted phenyl)propanoic acids with hydroxypropyl-β-cyclodextrin as a chiral additive:investigation of substituent influence on enantiorecognition[J].Chinese Journal of Chromatography,2017,435(5):544-550.