CNTs负载量对Span-PEG微泡超声显影的影响
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摘要
采用声振空化法制备了复合CNTs的Span-PEG超声造影剂微泡,使用激光粒度分析仪测定微泡的粒径分布,通过紫外分光光度法测定微泡中CNTs负载量,通过多普勒超声诊断仪观察复合微泡体内超声造影效果。所制备的复合微泡中CNTs负载量为1.42%时,微泡平均粒径410 nm;与生理盐水和SpanPEG微泡相比,复合CNTs的Span-PEG微泡明显提高了超声显像效果。
Span-PEG combining CNTs ultrasound contrast microbubble was prepared by using the acoustic cavitation method. The particle size distribution and ultrasonic imaging effect of the composite microbubbles were respectively detected by the laser particle size analyzer and doppler ultrasound instrument, and the loading efficiency of CNTs in the composite microbubbles was measured through ultraviolet spectroscopy. The average particle size of composite microbubble was 410 nm, the load capacity of CNTs in composite microbubble was 1.42%. Compared with normal saline and Span-PEG microbubble, Span-PEG combining CNTs microbubble obviously improved the effect of the ultrasonic imaging.
Span-PEG combining CNTs ultrasound contrast microbubble was prepared by using the acoustic cavitation method. The particle size distribution and ultrasonic imaging effect of the composite microbubbles were respectively detected by the laser particle size analyzer and doppler ultrasound instrument, and the loading efficiency of CNTs in the composite microbubbles was measured through ultraviolet spectroscopy. The average particle size of composite microbubble was 410 nm, the load capacity of CNTs in composite microbubble was 1.42%. Compared with normal saline and Span-PEG microbubble, Span-PEG combining CNTs microbubble obviously improved the effect of the ultrasonic imaging.
引文
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[3]侍海丽.多功能碳纳米管和氧化石墨烯的制备及其在生物成像和药物传输中的应用[D].上海师范大学,2014.
[4]Rathod V,Tripathi R,Joshi P,et al.Paclitaxel encapsulation into dual-functionalized multi-walled carbon nanotubes[J].AAPSPharm Sci Tech,2019,7;20(2):51,DOI:10.1208/s12249-018-1218-6.
[5]Mulvey J J,Littmann E R,Ling L L,et al.The effects of aminemodified single-walled carbon nanotubes on the mouse microbiota[J].International Journal of Nanomedicine,2018,13:5 275-5 286.
[6]Delogu L G,Vidili G,Venturelli E,et al.Functionalized multiwalled carbon nanotubes as ultrasound contrast agents[J].PNAS,2012,109(41):16 612-16 617.
[7]Gu F F,Hu C L,Xia Q M,et al.Aptamer-conjugated multi-walled carbon nanotubes as a new targeted ultrasound contrast agent for the diagnosis of prostate cancer[J].J Nanopart Res,2018,20(11):303.