摘要
目的:研究MPN计数法对肉类食品检测金黄色葡萄球菌的影响。方法:选择2017年5月至2018年12月期间,每月在武汉市农贸市场购买的生猪肉、生牛肉、禽肉类及散装熟肉4类肉类食品作为研究对象,分别利用MPN计数法检测其中的金黄色葡萄球菌,观察其检测结果。结果:经过MPN计数法检测后,其中生猪肉有10份阳性,检出率26.32%;生牛肉有10份阳性,检出率31.25%;禽肉类有26份阳性,检出率50.98%;散装熟肉有19份阳性,检出率35.19%。结论:MPN计数法在肉类食品检测金黄色葡萄球菌中具有重要意义,检出率较高,具有临床推广使用的价值。
Objective: To study the effect of MPN counting method on the detection of Staphylococcus aureus in meat products. Methods: From May 2017 to December 2018, four kinds of raw meats such as raw pork, raw beef, poultry and bulk cooked meat purchased at the farmer's market in this city were used as research objects, and were detected by MPN counting method. Among them, Staphylococcus aureus, the results of the observation period. Results: All meats were tested by MPN counting method, among which 10 were positive for raw pork, the detection rate was 26.32%; 10 were positive for raw beef, the detection rate was 31.25%; 26 were positive for poultry meat, the detection rate was 50.98%; 19 cooked meats were positive, and the detection rate was 35.19%. Conclusion: MPN counting method is of great significance in the detection of Staphylococcus aureus in meat foods. The detection rate is high and it has the value of clinical promotion.
引文
[1]王志琴,神学慧.镇江丹徒区市售凉菜与熟肉中金黄色葡萄球菌与大肠菌群污染状况分析[J].热带病与寄生虫学,2017,15(4):224-225.
[2]李昊翔.肉类食品中金黄色葡萄球菌耐药性的检测[J].黑龙江生态工程职业学院学报,2018,31(6):27-28,62.
[3]唐廷廷,王利娜,林华,等.生鲜畜禽肉中金黄色葡萄球菌风险评估研究进展[J].肉类研究,2017,31(7):67-72.
[4]曹建平,韩丹,王健,等.食品中金黄色葡萄球菌定量检测方法比较研究[J].安徽农业科学,2015,43(8):242-243,245.
[5]容冬丽,吴清平,吴诗,等.我国部分地区即食食品和蔬菜中金黄色葡萄球菌污染分布及耐药和基因分型情况[J].微生物学报,2018,58(2):314-323.