类香味细菌与巨大芽孢杆菌解离煤矸石应用研究
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摘要
煤矸石是煤炭生产过程中产生的固体废弃物,常以露天堆放形式贮存,占用大量土地,对周边环境造成了严重污染,目前未得到任何有效应用.利用微生物细菌解离煤矸石,释放其中的有效成分,制备微生物复合肥,是煤矸石应用研究的热点之一.细菌解离煤矸石,研究得比较多,具有良好应用前景的是巨大芽孢杆菌.研究发现,从风化煤矸石中培育提取出的类香味细菌,具有很好的解磷效果,其解磷效果优于传统解磷细菌巨大芽孢杆菌.实验结果表明,该菌株在接菌量为50mL,培养天数为2d,煤矸石粒径大小为140目以及体系pH为7时,获得理想的煤矸石解离结果.新型解磷细菌的开发,拓展了解磷微生物细菌的范围,获得了更为高效的解磷微生物,使煤矸石资源化应用成为可能,对环境保护和可持续性发展具有重要意义.
Coal gangue,a solid waste,is produced during the process of coal production.Usually,coal gangue is stored in open air and occupies a large amount of land,and it causes serious pollution to the surrounding environment.Currently,it has not been used effectively.It is one of the hot topics about the application of coal gangue by using microoganism to dissociate coal gangue and releasing the active component in it.It has many studies about the bacteria dissociate coal gangue,but the Bacillus megaterium has a good prospect of application.It is studied that the Myroidesodoratimimusstrain bred and extracted from the decomposed coal gangue has a good phosphate-solubilizing effect and it is better than the traditional Bacillus megaterium.The results showed that this kind of bacteria can make the coal gangue possess the best performance when the bacteria are in the condition of the particle size of coal gangue is 140 mesh,system pH 7,the inoculation amount of 50 mL and the incubation time for 2 days.The exploitation of new phosphate-solubilizing bacteria has expanded the scope of phosphate-solubilizing microorganism and get more effective phosphate-solubilizing microorganism,which makes the reclamation of coal gangue possible and this is of great significance for environmental protection and sustainable development.
Coal gangue,a solid waste,is produced during the process of coal production.Usually,coal gangue is stored in open air and occupies a large amount of land,and it causes serious pollution to the surrounding environment.Currently,it has not been used effectively.It is one of the hot topics about the application of coal gangue by using microoganism to dissociate coal gangue and releasing the active component in it.It has many studies about the bacteria dissociate coal gangue,but the Bacillus megaterium has a good prospect of application.It is studied that the Myroidesodoratimimusstrain bred and extracted from the decomposed coal gangue has a good phosphate-solubilizing effect and it is better than the traditional Bacillus megaterium.The results showed that this kind of bacteria can make the coal gangue possess the best performance when the bacteria are in the condition of the particle size of coal gangue is 140 mesh,system pH 7,the inoculation amount of 50 mL and the incubation time for 2 days.The exploitation of new phosphate-solubilizing bacteria has expanded the scope of phosphate-solubilizing microorganism and get more effective phosphate-solubilizing microorganism,which makes the reclamation of coal gangue possible and this is of great significance for environmental protection and sustainable development.
引文
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[8]程勇,袁峰,谢承卫.煤矸石中炭的浮选及煤矸石活化的研究[J].粉煤灰综合利用,2010.34-36.Cheng Yong,Yuan Feng,Xie Chengwei.Study on the flotation of coal in coal gangue and activation of coal gangue[J].Fly Ash Comprehensive Utilization,2010.34-36.(in Chinese)
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[10]钟传青,黄为一.不同种类解磷微生物的溶磷效果及磷酸酶活性的变化[J].土壤学报,2005(2):286-294.Zhong Chuanqing,,Huang Weiyi.Comparison In P-solubilizing effects between different P-solubilizing microbes and variation of activities of their phosphatases[J].Acta Pedologica Sinica,2005(2):286-294.(in Chinese)
[11]Sundara R,Sinha M K.Phosphate dissolving microorgamisms in the Rhizosphere and soil[J].India J.Agric.Sci.,1963,33(4):272-278.
[12]Molla,Chowdhury A.Microbial mineralization of organic phosphate in soil[J].Plant and Soil,1984,78:393-399.
[13]Paul N B,Sundara R.Phosphate dissolving bacteria in the rhizosphere of some cultivated hegumes[J].Plant and Soil,1971,35:127-132.
[14]袁向芬,程帆,杨艳梅,等.外界条件对硅酸盐细菌分解高硫煤矸石的影响[J].贵州农业科学,2014,42(2):207-210.Yuan Xiangfen,Cheng fan,Yang Yanmei,et al.Influence of external conditions on silicate bacteria decomposing high-sulfur coal Gangue[J].Guizhou Agricultural Sciences,2014,42(2):207-210.(in Chinese)
[15]袁向芬,谢承卫.利用巨大芽孢杆菌制备高硫煤矸石肥料[J].环境工程学报,2015(2):946-950.Yuan Xiangfen,Xie Chengwei.Preparation of highsulfur coal gangue fertilizer by bacillus megaterium[J].Chinese Journal of Environmental Engineering,2015(2):946-950.(in Chinese)
[16]伍昌维,陈泉.贵州省煤矸石的组成特征及综合利用途径探索[J].贵州化工,2013,38(3):6-8.Wu Changwei,Chen quan.Discussion into constitutions characteristics of Guizhou coal Gangue and its comprehensive utilization[J].Guizhou ChemicalIndustry,2013,38(3):6-8.(in Chinese)
[17]袁向芬,程帆,杨艳梅,等.两种细菌处理高硫煤矸石制备肥料的研究[J].硅酸盐通报,2014,33(2):302-307.Yuan Xiangfen,Cheng Fan,Yang Yanmei,et al.Study on the preparation of high-sulfur coal Gangue fertilizer worked by two bacteria[J].Bulletin of the Chinese Ceramic Society,2014,33(2):302-307.(in Chinese)
[18]钟燕,杨艳梅,谢承卫.利用巨大芽孢杆菌处理高硫和低硫煤矸石制备肥料的研究[J].贵州师范学院学报,2015,31(12):18-25.Zhong Yan,Yang Yanmei,Xie Chengwei.Study on the preparation of high-sulfur and low-sulfur coal gangue fertilizers by bacillus megaterium[J].Journal of Guizhou Education University,2015,31(12):18-25.(in Chinese)
[19]杜森,高祥照.土壤分析技术规范[M].北京:中国农业出版社,2009.
[2]罗海波,刘方,龙健,等.贵州山区煤矸石堆场重金属迁移对水稻土质量的影响及评价[J].水土保持学报,2010(3):71-74.Luo Haibo,Liu Fang,Long Jian,et al.Impacts of heavy metal migration from Gangue yard on paddy soil quality in hilly areas of Guizhou province and its evaluation[J].Journal of Soil and Water Conservation,2010(3):71-74.(in Chinese)
[3]覃宇容.煤矸石淋溶水对地下水影响及防治对策探讨[J].资源节约与环保,2015(5):169.Qin Yurong.Discussion on the effect of coal gangue leaching water on groundwater and prevention countermeasures[J].Resources Economization and Environmental Protection,2015(5):169.(in Chinese)
[4]祁星鑫,王晓军,黎艳,等.新疆主要煤区煤矸石的特性研究及其利用建议[J].煤炭学报,2010,7(13):1197-1201.Qi Xingxin,Wang Xiaojun,Li Yan,et al.Study on properties of the coal gangues from Xinjiang main coal mine regions and their utilization suggestions[J].Journsl Of China Coal Society,2010,7(13):1197-1201.(in Chinese)
[5]李静,温鹏飞,何振嘉.煤矸石的危害性及综合利用的研究进展[J].煤矿机械,2017(11):128-130.Li Jing,Wen Pengfei,He Zhenjia.Research progress harmfulness and comprehensive utilization of coal Gangue[J].Coal Mine Machinery,2017(11):128-130.(in Chinese)
[6]温贺兴,吴永,肖玉峰,等.神东煤田某露天矿煤矸石利用探讨[J].煤炭加工与综合利用,2014(3):72-74.Wen Hexing,Wu yong,Xiao Yufeng,et al.Discussion on the utilization of coal gangue in an open-pit mine in shendong coalfield[J].Coal Processing and Comprehensive Utilization,2014(3):72-74.(in Chinese)
[7]孙春宝,张金山,董红娟,等.煤矸石及其国内外综合利用[J].煤炭技术,2016,35(3):286-288.Sun Chunbao,Zhang Jinshan,Dong Hongjuan.et al.Coal gangue and its comprehensive utilization at home and abroad[J].Coal Technology,2016,35(3):286-288.(in Chinese)
[8]程勇,袁峰,谢承卫.煤矸石中炭的浮选及煤矸石活化的研究[J].粉煤灰综合利用,2010.34-36.Cheng Yong,Yuan Feng,Xie Chengwei.Study on the flotation of coal in coal gangue and activation of coal gangue[J].Fly Ash Comprehensive Utilization,2010.34-36.(in Chinese)
[9]袁峰,张国义,谢承卫.黔西南煤矸石制备4A分子筛研究[J].环保科技,2009(1):4-7.Yuan Feng,Zhang Guoyi,Xie Chengwei.Investigation of 4A molecular sieve preparation from gangue in southwest guizhou province[J].Environmental Protection and Technology,2009(1):4-7.(in Chinese)
[10]钟传青,黄为一.不同种类解磷微生物的溶磷效果及磷酸酶活性的变化[J].土壤学报,2005(2):286-294.Zhong Chuanqing,,Huang Weiyi.Comparison In P-solubilizing effects between different P-solubilizing microbes and variation of activities of their phosphatases[J].Acta Pedologica Sinica,2005(2):286-294.(in Chinese)
[11]Sundara R,Sinha M K.Phosphate dissolving microorgamisms in the Rhizosphere and soil[J].India J.Agric.Sci.,1963,33(4):272-278.
[12]Molla,Chowdhury A.Microbial mineralization of organic phosphate in soil[J].Plant and Soil,1984,78:393-399.
[13]Paul N B,Sundara R.Phosphate dissolving bacteria in the rhizosphere of some cultivated hegumes[J].Plant and Soil,1971,35:127-132.
[14]袁向芬,程帆,杨艳梅,等.外界条件对硅酸盐细菌分解高硫煤矸石的影响[J].贵州农业科学,2014,42(2):207-210.Yuan Xiangfen,Cheng fan,Yang Yanmei,et al.Influence of external conditions on silicate bacteria decomposing high-sulfur coal Gangue[J].Guizhou Agricultural Sciences,2014,42(2):207-210.(in Chinese)
[15]袁向芬,谢承卫.利用巨大芽孢杆菌制备高硫煤矸石肥料[J].环境工程学报,2015(2):946-950.Yuan Xiangfen,Xie Chengwei.Preparation of highsulfur coal gangue fertilizer by bacillus megaterium[J].Chinese Journal of Environmental Engineering,2015(2):946-950.(in Chinese)
[16]伍昌维,陈泉.贵州省煤矸石的组成特征及综合利用途径探索[J].贵州化工,2013,38(3):6-8.Wu Changwei,Chen quan.Discussion into constitutions characteristics of Guizhou coal Gangue and its comprehensive utilization[J].Guizhou ChemicalIndustry,2013,38(3):6-8.(in Chinese)
[17]袁向芬,程帆,杨艳梅,等.两种细菌处理高硫煤矸石制备肥料的研究[J].硅酸盐通报,2014,33(2):302-307.Yuan Xiangfen,Cheng Fan,Yang Yanmei,et al.Study on the preparation of high-sulfur coal Gangue fertilizer worked by two bacteria[J].Bulletin of the Chinese Ceramic Society,2014,33(2):302-307.(in Chinese)
[18]钟燕,杨艳梅,谢承卫.利用巨大芽孢杆菌处理高硫和低硫煤矸石制备肥料的研究[J].贵州师范学院学报,2015,31(12):18-25.Zhong Yan,Yang Yanmei,Xie Chengwei.Study on the preparation of high-sulfur and low-sulfur coal gangue fertilizers by bacillus megaterium[J].Journal of Guizhou Education University,2015,31(12):18-25.(in Chinese)
[19]杜森,高祥照.土壤分析技术规范[M].北京:中国农业出版社,2009.