超声波辅助水酶法提取胡麻油工艺条件的研究
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摘要
以胡麻籽为原料,研究了超声波辅助水酶法提取胡麻油的工艺条件。研究结果表明:最佳复合酶为酸性蛋白酶和纤维素酶(2∶1),最佳酶解条件为加酶量0.20 g(20 g底物)、酶解pH值5.0、酶解温度50℃、酶解时间1 h,在此条件下提油率为75.1%;当在超声温度45℃、超声频率45 kHz下处理15 min提油率可升到81.1%。
Taking flaxseed as raw material,the ultrasound-assisted aqueous enzymatic extraction of flaxseed oil process was researched.The results show that the best recombination of enzymes are acid protease and cellulase(2∶1),and the optimal conditions for hydrolysis are as follows:enzyme dosage 0.20 g(20 g substrate),hydrolysis pH value 5.0,reaction temperature50 ℃ and hydrolysis time 1 h,then flaxseed oil extraction rate is 75.1% under these conditions.While the oil extraction rate can rise 81.1% with assist of the ultrasound extraction method using the following parameters:ultrasonic temperature 45℃for 15 min,ultrasonic frequency 45 kHz.
Taking flaxseed as raw material,the ultrasound-assisted aqueous enzymatic extraction of flaxseed oil process was researched.The results show that the best recombination of enzymes are acid protease and cellulase(2∶1),and the optimal conditions for hydrolysis are as follows:enzyme dosage 0.20 g(20 g substrate),hydrolysis pH value 5.0,reaction temperature50 ℃ and hydrolysis time 1 h,then flaxseed oil extraction rate is 75.1% under these conditions.While the oil extraction rate can rise 81.1% with assist of the ultrasound extraction method using the following parameters:ultrasonic temperature 45℃for 15 min,ultrasonic frequency 45 kHz.
引文
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[2]Afaff KamaI-eldin,Lars-ke Appelqvist.The effects of extraction methods on sesame oilstability[J].JAOCS,1995,72(8):967-969.
[3]崔宝玉,刘喆,阚侃,等.亚麻油提取工艺的研究进展[J].中国麻业科学,2010,32(4):238-241.
[4]牛艳,王彩艳,左忠,等.不同加工工艺对胡麻油过氧化值的影响[J].粮油加工,2009(9):74-75.
[5]江连洲,李杨,王妍,等.水酶法提取大豆油的研究进展[J].食品科学,2013,34(9):348-350.
[6]郭兴凤,陈定刚,孙金全,等.水酶法提油技术概述[J].粮油加工,2007(5):70-72.
[7]吴素萍,刘敦华.水酶法提取胡麻籽油工艺条件的研究[J].中国油脂,2007,32(2):19-22.
[8]王明星,王月华,贾婷婷,等.水酶法提取玉米油的工艺研究进展[J].粮食与食品工业,2012,19(6):5-7.
[9]章绍兵,王璋.水酶法从菜籽中提取油及水解蛋白的研究[J].农业工程学报,2007,23(9):213-219.