摘要
为充分利用对虾加工副产物资源,采用溶剂提取法,探讨以对虾虾头为原料提取磷脂的最佳工艺和影响因素。以磷脂纯度为指标,通过单因素实验与正交实验,考察料液比、乙醇体积分数、提取时间及提取次数对磷脂提取的影响,制定出虾头磷脂的最佳提取工艺。结果表明,虾头磷脂的最佳提取工艺条件为:料液比1∶6,采用90%乙醇溶液作为提取液提取虾头2次,每次提取6 h。在最佳提取工艺条件下,虾头提取物中磷脂纯度为45.81%。该提取工艺简单、污染少、提取效率高,为低附加值海产品加工副产物的高值化利用提供有效途径。
In order to make full use of the byproducts of shrimp processing,the optimal process and influencing factors of extraction phospholipids from shrimp head were studied by solvent extraction method.The effects of ratio of material to solvent,volume fraction of ethanol,extraction time and extraction times on the extraction of phospholipids were researched by single factor experiment and orthogonal experiment with phospholipids purity as index to obtain the optimal extraction process of phospholipids from shrimp head. The results showed that the optimal extraction conditions of phospholipids from shrimp head were obtained as follows: ratio of material to solvent 1∶ 6,volume fraction of ethanol 90%,extraction time 6 h and extraction twice. Under these conditions,the phospholipids purity in shrimp head extract was45. 81%. The process had advantages of simple,less pollution and high extraction efficiency,which provided an effective way for the high value utilization of low-value-added seafood byproducts.
引文
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