HPLC法测定壶瓶枣中环磷酸腺苷含量的研究
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摘要
建立了用高效液相色谱仪测定壶瓶枣中环磷酸腺苷(cAMP)含量的方法,采用Sino Chrom ODS-BP色谱柱(5μm,4. 6 mm×200. 0 mm),柱温30℃,流动相为磷酸二氢钾溶液-甲醇(80∶20),流速为1 m L/min;进样量10μL;检测波长259 nm.环磷酸腺苷在10. 0μg/m L～50. 0μg/m L浓度范围内,浓度和检测峰面积呈良好的线性关系,R~2=0. 999 076 28.该方法准确度高,快速简便,适用于壶瓶枣中c AMP的检测。
To establish a method for the determination of the concent of cyclic adenodsine monophosphate( cAMP) in Zizyphus jujuba cv. Huping by high performance liquid chromatography,the chromatographic analysis was carried out by Sino Chrom ODS-BP( 5μm,4. 6 mm × 200. 0 mm) and the column temperature was 30℃. The mobile phase was consisted of potassium dihydrogen phosphate solution and methanol( 80 ∶ 20) at a flow rate of 1 m L/min. The sampling volume was10 m L and detection wavelength was 259 nm. There was a good linear relationship between the concentration of c AMP and detection peak area in the range of 1. 0 μg/m L ～ 50. 0 μg/m L,and the value of R~2 was 0. 999 076 28. This method was high accuracy,rapid and simple. It was suitable for the detection of c AMP in Zizyphus jujuba cv. Huping.
To establish a method for the determination of the concent of cyclic adenodsine monophosphate( cAMP) in Zizyphus jujuba cv. Huping by high performance liquid chromatography,the chromatographic analysis was carried out by Sino Chrom ODS-BP( 5μm,4. 6 mm × 200. 0 mm) and the column temperature was 30℃. The mobile phase was consisted of potassium dihydrogen phosphate solution and methanol( 80 ∶ 20) at a flow rate of 1 m L/min. The sampling volume was10 m L and detection wavelength was 259 nm. There was a good linear relationship between the concentration of c AMP and detection peak area in the range of 1. 0 μg/m L ～ 50. 0 μg/m L,and the value of R~2 was 0. 999 076 28. This method was high accuracy,rapid and simple. It was suitable for the detection of c AMP in Zizyphus jujuba cv. Huping.
引文
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[2]杨芳媛,赵智慧,刘孟军,等.枣中环磷酸腺苷的研究进展[J].塔里木大学学报,2016(3):119-121.
[3]LIU Z Q,JIANG Y F,YUE X L,et al.Effect of Naoreqing oral liguid on c AMP content in hypothalamus and CSF and AVP content in ventral septal area of endotoxininduced febrile rabbits[J].Chinese Journal of Pathophysiology,2003,19(11):1 507.
[4]Zaccolo M.c AMP signal transduction in the heart:understanding spatial control for the development of novel therapeutic strategies[J].British journal of pharmacology,2009,158(1):50-60.
[5]Cho E A,Juhnn Y S.The c AMP signaling system inhibits the repair ofγ-ray-induced DNA damage by promoting Epac1-mediated proteasomal degradation of XRCC1 protein in human lung cancer cells[J].Biochemical and biophysical research communications,2012,422(2):256-262.
[6]党富民,代文婷,吴洪斌,等.新疆红枣中环磷酸腺苷含量的LC-MS/MS法测定研究[J].食品工业,2018,39(10):286-288.
[7]杨雯.红枣粉加工工艺及稳定性研究[D].西安:陕西科技大学,2012.
[8]崔志强,孟宪军,王传杰.HPLC法测定冬枣环磷酸腺苷含量[J].食品研究与开发,2006,27(7):158-163.
[9]孟伊娜,马燕,邹淑萍,等.不同成熟期骏枣贮藏期环磷酸腺苷变化研究[J].新疆农业科学,2016,53(8):1 436-1 443.
[10]孟伊娜,马燕,邹淑萍,等.不同成熟期骏枣贮藏期环磷酸腺苷变化研究[J].新疆农业科学,2016,53(8):1 436-1 443.
[11]高侨君.金丝小枣环磷酸腺苷制备技术研究[D].泰安:山东农业大学,2015.
[12]任卫合,王丽萍,郭鹏辉.三种不同大枣中环磷酸腺苷的提取与含量的比较[J].甘肃科技纵横,2017,46(6):21-23.