酶联免疫法测定水产品中己烯雌酚残留量
|
摘要
采用酶联免疫法测定水产品中残留己烯雌酚的含量,并采用液相色谱-质谱联用法验证阳性结果。实验结果表明,该方法对水产品中己烯雌酚的检出限为0.35μg/kg,回收率达77%～91%,具有良好的灵敏度和准确度。
The content of diethylstilbestrol(DES)residues in aquatic products was determined by enzyme linked immunosorbent assay(ELISA),and the positive results were verified by liquid chromatography-mass spectrometry(LC-MS).The final test results show that the detection limit of DES in aquatic products is 0.35 μg/kg and the recovery rate is 77%～91%. This method has good sensitivity and accuracy.
The content of diethylstilbestrol(DES)residues in aquatic products was determined by enzyme linked immunosorbent assay(ELISA),and the positive results were verified by liquid chromatography-mass spectrometry(LC-MS).The final test results show that the detection limit of DES in aquatic products is 0.35 μg/kg and the recovery rate is 77%～91%. This method has good sensitivity and accuracy.
引文
[1]DODDS E C,GOLDBERG L,LAWSON W,et al.Oestrogenicactivity of certain synthetic compounds [J].Nature,1938,141(3562):247-248.DOI:10.1038/141247b0.
[2]FAN Q J,WANG X H,ZHOU Q B,et al.Synthesis of artificial antigens of diethylstilbestrol and preparation of its antibody [J].Front Agric China,2010,4(2):188-194.DOI:10.1007/s11703-010-0097-8.
[3]HERBST A L,ULFELDER H,POSKANZER D C.Adenocarcinoma of the vagina:association of maternalstilbestrol therapy with tumor appearance in young women [J].N Engl J Med,1971,284(15):878-881.DOI:10.1056/NEJM197104222841604.
[4]邓省亮,李平,于洪侠,等.己烯雌酚残留检测技术的研究进展[J].中国畜牧杂志,2010,46(24):56-60.
[5]中华人民共和国农业部.中华人民共和国农业部公告第193号[EB/OL].[2018-05-12].http://jiuban.moa.gov.cn/zwllm/tzgg/gg/201104/t20110422_1976324.htm.
[6]中华人民共和国农业部.中华人民共和国农业部公告第176号[EB/OL].[2018-05-12].http://jiuban.moa.gov.cn/zwllm/tzgg/gg/201104/t20110422_1976307.htm.
[7]王东伟,陈建华,周磊.高效液相色谱法测定饲料中己烯雌酚含量的方法学考察[J].中国草食动物科学,2015,35(6):74-76.DOI:10.3969/j.issn.2095-3887.2015.06.024.
[8]应永飞,朱聪英,陈慧华,等.液相色谱-串联质谱法测定动物组织中1,2-二苯乙烯类药物残留的研究[J].质谱学报,2008,29(6):343-348.
[9]郭志峰,安秋荣,刘鹏岩.鸡蛋中己烯雌酚的GC/MS法分析测定[J].质谱学报,1997,18(3):36-40.
[10]HOFFMANN B,WEILER S.Investigations on the radioimmunological determination of stilbenes in tissue samples from pigs [J].Archiv Fur Lebensmittelhygiene,1986,37(6):137-142.
[11]中华人民共和国农业部.动物性食品中兽药最高残留限量[EB/OL].[2018-05-12].http://jiuban.moa.gov.cn/zwllm/tzgg/gg/200302/t20030226_59300.htm.
[2]FAN Q J,WANG X H,ZHOU Q B,et al.Synthesis of artificial antigens of diethylstilbestrol and preparation of its antibody [J].Front Agric China,2010,4(2):188-194.DOI:10.1007/s11703-010-0097-8.
[3]HERBST A L,ULFELDER H,POSKANZER D C.Adenocarcinoma of the vagina:association of maternalstilbestrol therapy with tumor appearance in young women [J].N Engl J Med,1971,284(15):878-881.DOI:10.1056/NEJM197104222841604.
[4]邓省亮,李平,于洪侠,等.己烯雌酚残留检测技术的研究进展[J].中国畜牧杂志,2010,46(24):56-60.
[5]中华人民共和国农业部.中华人民共和国农业部公告第193号[EB/OL].[2018-05-12].http://jiuban.moa.gov.cn/zwllm/tzgg/gg/201104/t20110422_1976324.htm.
[6]中华人民共和国农业部.中华人民共和国农业部公告第176号[EB/OL].[2018-05-12].http://jiuban.moa.gov.cn/zwllm/tzgg/gg/201104/t20110422_1976307.htm.
[7]王东伟,陈建华,周磊.高效液相色谱法测定饲料中己烯雌酚含量的方法学考察[J].中国草食动物科学,2015,35(6):74-76.DOI:10.3969/j.issn.2095-3887.2015.06.024.
[8]应永飞,朱聪英,陈慧华,等.液相色谱-串联质谱法测定动物组织中1,2-二苯乙烯类药物残留的研究[J].质谱学报,2008,29(6):343-348.
[9]郭志峰,安秋荣,刘鹏岩.鸡蛋中己烯雌酚的GC/MS法分析测定[J].质谱学报,1997,18(3):36-40.
[10]HOFFMANN B,WEILER S.Investigations on the radioimmunological determination of stilbenes in tissue samples from pigs [J].Archiv Fur Lebensmittelhygiene,1986,37(6):137-142.
[11]中华人民共和国农业部.动物性食品中兽药最高残留限量[EB/OL].[2018-05-12].http://jiuban.moa.gov.cn/zwllm/tzgg/gg/200302/t20030226_59300.htm.