海洋着色菌YL28对含沉积物的海水养殖水体氮污染去除效应的研究
|
摘要
用含有沉积物的氮化物污染实际水体作为测定体系,通过测定水体无机三态氮、总氮、COD、pH以及沉积物总氮、COD和对4种代谢酶活性的动力学过程的监测,评价了一株不产氧光合细菌——海洋着色菌(Marichromatium gracile)YL28对海水实际养殖水体脱氮效应。结果表明:水体本身具有一定的自净作用;与对照组相比,YL28组水体氨氮、亚硝氮、硝氮、沉积物总氮和COD的残留率明显降低(P<0.05),在6~10d内水体COD明显升高,水体总氮和pH未见显著差异。处理过程中,在特定的时间范围内,转化酶、蛋白酶、脲酶和脱氢酶活性明显升高(P<0.05),与沉积物总氮和COD去除规律相吻合。由此可见,添加YL28对污染海水养殖水体中无机三态氮、沉积物有机氮化物和有机碳化物具有明显的去除作用,本研究为海水养殖微生态水质修复剂的开发和应用奠定基础。
The actual mariculture nitrogen pollution water containing sediments was selected as the measurement system.Effects of a anoxygenic phototrophic bacterium Marichromatium gracile YL28 on the denitrification of actual mariculture water were studied by the method of kinetic process about inorganic nitrogen,total nitrogen(TN),COD and pH in overlying water,as well as TN,COD and metabolic activity of the four enzymes in sediment.Results showed that the water body itself had a certain self-purification effect;compared with control system,the residual rate of ammonia,nitrate and nitrite in water,TN and COD in sediment significantly decreased(P<0.05)in the YL28 system.The COD of YL28 system increased significantly in 6~10 days,and TN and pH were no significant difference.The activity of invertase,protease,urease and dehydrogenase increased significantly(P<0.05)in the specific time range of the process,which was consistent with the removal of TN and COD.It indicates that there is obvious removing effect for inorganic nitrogen in contaminated mariculture water and organic nitrides and carbides in sediment by adding YL28.This study lays the foundation for the development and application of micro-ecological water quality restorer preparation for mariculture.
The actual mariculture nitrogen pollution water containing sediments was selected as the measurement system.Effects of a anoxygenic phototrophic bacterium Marichromatium gracile YL28 on the denitrification of actual mariculture water were studied by the method of kinetic process about inorganic nitrogen,total nitrogen(TN),COD and pH in overlying water,as well as TN,COD and metabolic activity of the four enzymes in sediment.Results showed that the water body itself had a certain self-purification effect;compared with control system,the residual rate of ammonia,nitrate and nitrite in water,TN and COD in sediment significantly decreased(P<0.05)in the YL28 system.The COD of YL28 system increased significantly in 6~10 days,and TN and pH were no significant difference.The activity of invertase,protease,urease and dehydrogenase increased significantly(P<0.05)in the specific time range of the process,which was consistent with the removal of TN and COD.It indicates that there is obvious removing effect for inorganic nitrogen in contaminated mariculture water and organic nitrides and carbides in sediment by adding YL28.This study lays the foundation for the development and application of micro-ecological water quality restorer preparation for mariculture.
引文
[1]Díaz V,Ibá1ez R,Gómez P,et al.Kinetics of electrooxidation of ammonia-N,nitrites and COD from a recirculating aquaculture saline water system using BDD anodes[J].Water Res,2011,45(1):125-134.
[2]Corey P,Kim J K,Duston J,et al.Bioremediation potential of Palmaria palmata and Chondrus crispus(Basin Head):effect of nitrate and ammonium ratio as nitrogen source on nutrient removal[J].J Appl Phycol,2013,25(5):1349-1358.
[3]Jiang P,Zhao C G,Jia Y Q,et al.Inorganic nitrogen removal by a marine purple sulfur bacterium capable of growth on nitrite as sole nitrogen source[J].Microbiology China,2014,41(5):824-831.蒋鹏,赵春贵,贾雅琼,等.以亚硝氮为唯一氮源生长的海洋紫色硫细菌去除无机三态氮[J].微生物学通报,2014,41(5):824-831.
[4]Yusoff F M,Banerjee S,Khatoon H,et al.Biological approaches in management of nitrogenous compounds in aquaculture systems[J].Dyn Biochem Process Biotech Mol Biol,2011,5:21-31.
[5]Burford M A,Williams K C.The fate of nitrogenous waste from shrimp feeding[J].Aquaculture,2001,198(1):79-93.
[6]Zhou Q,Li K,Jun X,et al.Role and functions of beneficial microorganisms in sustainable aquaculture[J].Bioresour Technol,2009,100(16):3780-3786.
[7]Zhang Q H,Feng Y H,Wang J,et al.Study on the characteristics of the ammonia-nitrogen and residual feeds degradation in aquatic water by bacillus licheniformis[J].Acta Hydrobiologica Sinica,2011,35(3):498-503.张庆华,封永辉,王娟,等.地衣芽孢杆菌对养殖水体氨氮、残饵降解特性研究[J].水生生物学报,2011,35(3):498-503.
[8]Zhou X J,Chen K Z.Selection of one ammonia-degrading oceanic yeast and studies on its biological characteristic[J].Journal of Zhan Jiang Normal college,2007,28(6):100-103.周鲜娇,陈康振.降解氨氮的海洋酵母菌的筛选及其生物特性[J].湛江师范学院学报,2007,28(6):100-103.
[9]Zhang J S,Lu N,Tian C C,et al.Screening and identification of an efficient nitrogen-degrading strain and application of potential analysis[J].Journal of Fishery Sciences of China,2017,24(4):757-765.张家顺,吕娜,田长城,等.一株高效脱氮菌株的分离鉴定及应用潜力分析[J].中国水产科学,2017,24(4):757-765.
[10]Zhao J Y,Fu Y N,Zhao C G,et al.Identification and characterization of a purple sulfur bacterium from mangrove with rhodopin as predominant carotenoid[J].Acta Microbiologica Sinica,2011,51(10):1318-1325.赵江艳,傅英楠,赵春贵,等.一株高含玫红品的红树林海洋紫色硫细菌分离鉴定及特性[J].微生物学报,2011,51(10):1318-1325.
[11]Zhang X B,Zhu B T,Chan Z H,et al.Effect of organic carbons on the removal of inorganic nitrogens coexisting in marine aquaculture by a marine purple sulfur bacterium,Marichramatium gracile YL28[J].Microbiology China,2017,44(5):1017-1027.张晓波,朱笔通,产竹华,等.有机碳对海洋着色菌YL28去除无机三态氮的影响[J].微生物学通报,2017,44(5):1017-1027.
[12]Jiang P,Hong X,Zhao C G,et al.Reciprocal transformation of inorganic nitrogen by resting cells of Marichromatium gracile YL28[J].Journal of Huaqiao University(Natural Science),2015,36(2):185-189.蒋鹏,洪璇,赵春贵,等.海洋着色菌Marichromatium gracile YL28静息细胞对无机三态氮的相互转化作用[J].华侨大学学报(自然科学版),2015,36(2):185-189.
[13]Jiang P,Zhao C G,Jia Y Q,et al.Effects of nitrite on ammonia-nitrogen removal and nitrite-nitrogen as well as photopigment biosynthesis of Marichromatium gracile YL28[J].Microbiology China,2015,42(7):1216-1223.蒋鹏,赵春贵,贾雅琼,等.亚硝氮对海洋着色菌亚硝氮和氨氮去除以及光合色素合成的影响[J].微生物学通报,2015,42(7):1216-1223.
[14]Pfennig N,Trüper H G.The Family Chromatiaceae[M].The Prokaryotes.New York:Springer,1992:151-178.
[15]Wang X F,Wei F S,Qi W Q.Methods of the water and wastewater inspection and analysis[M].Beijing:China Environmental Press,2002:216-219.王心芳,魏复盛,齐文启.水和废水监测分析方法[M].北京:中国环境科学出版社,2002:216-219.
[16]Wen J,Zeng G M,An H,et al.Effects of zeolitemodified sediment on microbial biomass carbon and enzyme activity in soil[J].Journal of Agro-Environment Science,2017,36(2):302-307.文嘉,曾光明,安赫,等.改性沸石改良底泥对土壤中微生物生物量碳及酶活性的影响[J].农业环境科学学报,2017,36(2):302-307.
[17]Foss A,Imsland A K,Roth B,et al.Effects of chronic and periodic exposure to ammonia on growth and blood physiology in juvenile turbot(Scophthalmus maximus)[J].Aquaculture,2009,296(1):45-50.
[18]Zokaeifar H,Babaei N,Saad C R,et al.Administration of Bacillus subtilis strains in the rearing water enhances the water quality,growth performance,immune response,and resistance against Vibrio harveyi infection in juvenile white shrimp,Litopenaeus vannamei[J].Fish Shellfish Immun,2014,36(1):68-74.
[19]Huang H H,He L,Lei Y J,et al.Characterization of growth and ammonia degrading of a Bacillus strain under broth with low concentration of nitrogen source[J].Acta Scientiae Circumstantiae,2017,1-12.DOI:10.13671/j.hjkxxb.2017.0260黄海洪,贺莉,类延菊,等.一株芽孢杆菌在低氮源浓度培养基中的生长与氨氮降解特性[J].环境科学学报,2017,1-12.DOI:10.13671/j.hjkxxb.2017.0260
[20]Wen G,Wang B,Liu B,et al.Ammonia nitrogen removal efficiency from aquaculture water by Rhodopseudomonas palustris R-3[J].Hu Nan Agricultural Sciences,2017,(6):49-51.文刚,汪彬,刘标,等.沼泽红假单胞菌R-3去除水体中氨氮的特性研究[J].湖南农业科学,2017,(6):49-51.
[21]Zhao M W,Liu Q.Research on the effect of water purification by Bacillus subtili probiotics and sodium humate mixture[J].Journal of Aquaculture,2016,37(4):23-26.赵铭武,刘青.枯草芽孢杆菌与腐植酸钠合剂净水效果的研究[J].水产养殖.2016,37(4):23-26.
[22]Liu P P,Qiu L P.Research progress of large-scale marine aquacultu rewaste water treatment technology[J].Industrial Water&Wastewater,2016,47(2):1-4.刘盼盼,邱立平.规模化海水养殖废水处理技术研究进展[J].工业用水与废水,2016,47(2):1-4.
[23]Zhu S M,Deng Y L,Ruan Y J,et al.Biological denitrification using poly(butylene succinate)as carbon source and biofilm carrier for recirculating aquaculture system effluent treatment[J].Bioresour Technol,2015,192:603-610.
[24]Newaj-Fyzul A,Al-Harbi A H,Austin B.Developments in the use of probiotics for disease control in aquaculture[J].Aquaculture,2014,431:1-11.
[25]Qi Z,Zhang X,Boon N,et al.Probiotics in aquaculture of China—current state,problems and prospect[J].Aquaculture,2009,290(1):15-21.
[26]Jiang P,Zhao C G,Yang S P.Influences of low-molecular-weight organic carbon and nitrogen sources on growth and inorganic nitrogen removal by Marichromatium gracile strain YL28[J].Oceanologia Et Limnologia Sinica,2014,45(6):1218-1224.蒋鹏,赵春贵,杨素萍.小分子有机碳、氮源对海洋着色菌(Marichromatium gracile)生长和去除高浓度无机三态氮的影响[J].海洋与湖沼,2014,45(6):1218-1224.
[2]Corey P,Kim J K,Duston J,et al.Bioremediation potential of Palmaria palmata and Chondrus crispus(Basin Head):effect of nitrate and ammonium ratio as nitrogen source on nutrient removal[J].J Appl Phycol,2013,25(5):1349-1358.
[3]Jiang P,Zhao C G,Jia Y Q,et al.Inorganic nitrogen removal by a marine purple sulfur bacterium capable of growth on nitrite as sole nitrogen source[J].Microbiology China,2014,41(5):824-831.蒋鹏,赵春贵,贾雅琼,等.以亚硝氮为唯一氮源生长的海洋紫色硫细菌去除无机三态氮[J].微生物学通报,2014,41(5):824-831.
[4]Yusoff F M,Banerjee S,Khatoon H,et al.Biological approaches in management of nitrogenous compounds in aquaculture systems[J].Dyn Biochem Process Biotech Mol Biol,2011,5:21-31.
[5]Burford M A,Williams K C.The fate of nitrogenous waste from shrimp feeding[J].Aquaculture,2001,198(1):79-93.
[6]Zhou Q,Li K,Jun X,et al.Role and functions of beneficial microorganisms in sustainable aquaculture[J].Bioresour Technol,2009,100(16):3780-3786.
[7]Zhang Q H,Feng Y H,Wang J,et al.Study on the characteristics of the ammonia-nitrogen and residual feeds degradation in aquatic water by bacillus licheniformis[J].Acta Hydrobiologica Sinica,2011,35(3):498-503.张庆华,封永辉,王娟,等.地衣芽孢杆菌对养殖水体氨氮、残饵降解特性研究[J].水生生物学报,2011,35(3):498-503.
[8]Zhou X J,Chen K Z.Selection of one ammonia-degrading oceanic yeast and studies on its biological characteristic[J].Journal of Zhan Jiang Normal college,2007,28(6):100-103.周鲜娇,陈康振.降解氨氮的海洋酵母菌的筛选及其生物特性[J].湛江师范学院学报,2007,28(6):100-103.
[9]Zhang J S,Lu N,Tian C C,et al.Screening and identification of an efficient nitrogen-degrading strain and application of potential analysis[J].Journal of Fishery Sciences of China,2017,24(4):757-765.张家顺,吕娜,田长城,等.一株高效脱氮菌株的分离鉴定及应用潜力分析[J].中国水产科学,2017,24(4):757-765.
[10]Zhao J Y,Fu Y N,Zhao C G,et al.Identification and characterization of a purple sulfur bacterium from mangrove with rhodopin as predominant carotenoid[J].Acta Microbiologica Sinica,2011,51(10):1318-1325.赵江艳,傅英楠,赵春贵,等.一株高含玫红品的红树林海洋紫色硫细菌分离鉴定及特性[J].微生物学报,2011,51(10):1318-1325.
[11]Zhang X B,Zhu B T,Chan Z H,et al.Effect of organic carbons on the removal of inorganic nitrogens coexisting in marine aquaculture by a marine purple sulfur bacterium,Marichramatium gracile YL28[J].Microbiology China,2017,44(5):1017-1027.张晓波,朱笔通,产竹华,等.有机碳对海洋着色菌YL28去除无机三态氮的影响[J].微生物学通报,2017,44(5):1017-1027.
[12]Jiang P,Hong X,Zhao C G,et al.Reciprocal transformation of inorganic nitrogen by resting cells of Marichromatium gracile YL28[J].Journal of Huaqiao University(Natural Science),2015,36(2):185-189.蒋鹏,洪璇,赵春贵,等.海洋着色菌Marichromatium gracile YL28静息细胞对无机三态氮的相互转化作用[J].华侨大学学报(自然科学版),2015,36(2):185-189.
[13]Jiang P,Zhao C G,Jia Y Q,et al.Effects of nitrite on ammonia-nitrogen removal and nitrite-nitrogen as well as photopigment biosynthesis of Marichromatium gracile YL28[J].Microbiology China,2015,42(7):1216-1223.蒋鹏,赵春贵,贾雅琼,等.亚硝氮对海洋着色菌亚硝氮和氨氮去除以及光合色素合成的影响[J].微生物学通报,2015,42(7):1216-1223.
[14]Pfennig N,Trüper H G.The Family Chromatiaceae[M].The Prokaryotes.New York:Springer,1992:151-178.
[15]Wang X F,Wei F S,Qi W Q.Methods of the water and wastewater inspection and analysis[M].Beijing:China Environmental Press,2002:216-219.王心芳,魏复盛,齐文启.水和废水监测分析方法[M].北京:中国环境科学出版社,2002:216-219.
[16]Wen J,Zeng G M,An H,et al.Effects of zeolitemodified sediment on microbial biomass carbon and enzyme activity in soil[J].Journal of Agro-Environment Science,2017,36(2):302-307.文嘉,曾光明,安赫,等.改性沸石改良底泥对土壤中微生物生物量碳及酶活性的影响[J].农业环境科学学报,2017,36(2):302-307.
[17]Foss A,Imsland A K,Roth B,et al.Effects of chronic and periodic exposure to ammonia on growth and blood physiology in juvenile turbot(Scophthalmus maximus)[J].Aquaculture,2009,296(1):45-50.
[18]Zokaeifar H,Babaei N,Saad C R,et al.Administration of Bacillus subtilis strains in the rearing water enhances the water quality,growth performance,immune response,and resistance against Vibrio harveyi infection in juvenile white shrimp,Litopenaeus vannamei[J].Fish Shellfish Immun,2014,36(1):68-74.
[19]Huang H H,He L,Lei Y J,et al.Characterization of growth and ammonia degrading of a Bacillus strain under broth with low concentration of nitrogen source[J].Acta Scientiae Circumstantiae,2017,1-12.DOI:10.13671/j.hjkxxb.2017.0260黄海洪,贺莉,类延菊,等.一株芽孢杆菌在低氮源浓度培养基中的生长与氨氮降解特性[J].环境科学学报,2017,1-12.DOI:10.13671/j.hjkxxb.2017.0260
[20]Wen G,Wang B,Liu B,et al.Ammonia nitrogen removal efficiency from aquaculture water by Rhodopseudomonas palustris R-3[J].Hu Nan Agricultural Sciences,2017,(6):49-51.文刚,汪彬,刘标,等.沼泽红假单胞菌R-3去除水体中氨氮的特性研究[J].湖南农业科学,2017,(6):49-51.
[21]Zhao M W,Liu Q.Research on the effect of water purification by Bacillus subtili probiotics and sodium humate mixture[J].Journal of Aquaculture,2016,37(4):23-26.赵铭武,刘青.枯草芽孢杆菌与腐植酸钠合剂净水效果的研究[J].水产养殖.2016,37(4):23-26.
[22]Liu P P,Qiu L P.Research progress of large-scale marine aquacultu rewaste water treatment technology[J].Industrial Water&Wastewater,2016,47(2):1-4.刘盼盼,邱立平.规模化海水养殖废水处理技术研究进展[J].工业用水与废水,2016,47(2):1-4.
[23]Zhu S M,Deng Y L,Ruan Y J,et al.Biological denitrification using poly(butylene succinate)as carbon source and biofilm carrier for recirculating aquaculture system effluent treatment[J].Bioresour Technol,2015,192:603-610.
[24]Newaj-Fyzul A,Al-Harbi A H,Austin B.Developments in the use of probiotics for disease control in aquaculture[J].Aquaculture,2014,431:1-11.
[25]Qi Z,Zhang X,Boon N,et al.Probiotics in aquaculture of China—current state,problems and prospect[J].Aquaculture,2009,290(1):15-21.
[26]Jiang P,Zhao C G,Yang S P.Influences of low-molecular-weight organic carbon and nitrogen sources on growth and inorganic nitrogen removal by Marichromatium gracile strain YL28[J].Oceanologia Et Limnologia Sinica,2014,45(6):1218-1224.蒋鹏,赵春贵,杨素萍.小分子有机碳、氮源对海洋着色菌(Marichromatium gracile)生长和去除高浓度无机三态氮的影响[J].海洋与湖沼,2014,45(6):1218-1224.