一株拮抗黄曲霉的海洋微生物的筛选与鉴定
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摘要
以北冰洋海域沉积物为样本,分离筛选具有抗黄曲霉特性的海洋微生物菌株。采用稀释涂布平板法分离出海洋微生物单菌落,滤纸片扩散法筛选出对黄曲霉具有拮抗作用的菌株,将抑菌效果最好的菌株进行16S r DNA鉴定,并研究其抑菌谱。实验得到1株抑制黄曲霉效果最佳的菌株(26号菌株),其抑菌圈直径为33. 26 mm,鉴定结果为芽孢杆菌(Bacillus),它对大肠杆菌、鼠伤寒沙门氏菌、副溶血弧菌、金黄色葡萄球菌、铜绿假单胞菌和肺炎克雷伯杆菌都表现出一定的抑菌效果,是一株广谱抗菌的海洋细菌。
Strains of marine microorganisms against Aspergillus flavus were isolated and identified from the Arctic Ocean sediment samples. Strains of marine microorganisms were separated by dilution-plate method and screened by filter paper diffusion method. The strain with the best inhibiting effect was identified by 16 S r DNA identification,whose antibacterial spectrum was also studied. In the experiment,one strain with the best inhibiting effect,namely No. 26,was screened and selected. The average diameter of the inhibition zone was 33. 26 mm. It belongs to Bacillus. It showed some inhibiting effects against Escherichia coli,Salmonella typhimurium,Vibrio parahaemolyticu,Staphylococcus aureus,Pseudomonas aeruginosa and Klebsiella pneumoniae,which is a marine bacterium with a broad antibacterial spectrum.
Strains of marine microorganisms against Aspergillus flavus were isolated and identified from the Arctic Ocean sediment samples. Strains of marine microorganisms were separated by dilution-plate method and screened by filter paper diffusion method. The strain with the best inhibiting effect was identified by 16 S r DNA identification,whose antibacterial spectrum was also studied. In the experiment,one strain with the best inhibiting effect,namely No. 26,was screened and selected. The average diameter of the inhibition zone was 33. 26 mm. It belongs to Bacillus. It showed some inhibiting effects against Escherichia coli,Salmonella typhimurium,Vibrio parahaemolyticu,Staphylococcus aureus,Pseudomonas aeruginosa and Klebsiella pneumoniae,which is a marine bacterium with a broad antibacterial spectrum.
引文
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[8]王硕,孟庆丽,武天娇,等.抗金葡菌深海真菌的分离鉴定及抑菌谱研究[J].生物技术,2015,25(2):169-172.
[9]李虹宇,张公亮,侯红漫,等.仿刺参相关微生物对致病灿烂弧菌的拮抗及机理研究[J].食品工业,2012,33(9):117-120.
[2]KONG Q,CHI C,YU J J,et al. The inhibitory effects of Bacillus Megaterium on aflatoxin and cyclopiazonic acid biosynthetic pathway gene expression in Aspergillus flavus[J]. Applied Microbiology and Biotechnology,2014,98:5161-5172.
[3]王威,谢岩黎.降解黄曲霉毒素B1菌株发酵工艺的研究[J].河南工业大学学报(自然科学版),2018,39(3):76-82.
[4]张舒月,郑婧萱,郭晓军,等.青贮用拮抗黄曲霉并降解黄曲霉毒素菌株的筛选与鉴定[J].中国饲料,2018,3:26-29.
[5]宫小明,马荣桧,孙军,等.黄曲霉毒素生物防控菌的筛选及鉴定[J].微生物学杂志,2015,35(2):103-108.
[6]孔青,刘奇正,于方塘,等. 1株海洋芽孢杆菌抑制黄曲霉生长和毒素合成的研究[J].浙江大学学报(农业与生命科学版),2010,36(4):387-392.
[7]张静楠,聂灿玉,赵胜楠,等.抗白色念珠菌的海洋微生物的分离与鉴定[J].天津农业科学,2016,22(1):79-81.
[8]王硕,孟庆丽,武天娇,等.抗金葡菌深海真菌的分离鉴定及抑菌谱研究[J].生物技术,2015,25(2):169-172.
[9]李虹宇,张公亮,侯红漫,等.仿刺参相关微生物对致病灿烂弧菌的拮抗及机理研究[J].食品工业,2012,33(9):117-120.