DHA通过抑制氧化应激反应减轻七氟烷所致神经元损伤
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摘要
目的:观察二十二碳六烯酸(DHA)是否通过抑制氧化应激反应减轻七氟烷所致神经元损伤。方法:HT22小鼠海马神经元分为Con组、DHA组和Sevo组和DHA+Sevo组,药物处理各组细胞24 h后,在倒置相差显微镜下拍照记录各组细胞形态改变,采用MTT法检测神经元存活情况,检测各组培养基中LDH, NO, SOD及MDA的含量。结果:CON组和DHA组细胞形态正常,Sevo组细胞皱缩,胞体破裂,正常形态消失,而DHA+Sevo组细胞形态基本正常,皱缩破裂的细胞较少;与CON组和DHA组相比,Sevo组HT22细胞存活率下降至CON组的25.79%,培养液中LDH的漏出量显著增加至CON组的400.15%,SOD活力下降至CON组的30.96%,培养液中NO及MDA的含量分别增加至CON组的507.62%和342.15%(P<0.05);而与Sevo组相比,DHA+Sevo组HT22细胞存活率增加至CON组的75.68%,培养液中LDH的漏出量下降至CON组的175.68%,SOD活力增加至CON组的70.48%,培养液中NO及MDA的含量分别下降至CON组的355.80%和192.27%(P<0.05)。结论:DHA通过抗氧化应激反应减轻七氟烷对HT22细胞的损伤。
Objective: To observe whether DHA ameliorate sevoflurane-induced neuronal damage and explore the possible mechanism. Methods: Cells were assigned to Con group, DHA group, Sevo group and DHA+Sevo group. After treated, the cell morphology changes were photographed under inverted phase contrast microscope, and cell suvival was assessed by MTT assay; then the medium was used to assess the concentration of LDH, SOD, NO and MDA. Results: In CON and DHA groups, the cell morphology was normal,the cells in Servo group were collapsed, the cell body ruptured and the normal morphology disappeared. However, the cell morphology in DHA+Sevo group was normal and there were fewer rupture cells. Compared with CON group and DHA group, the survival rate of HT22 cells in Sevo group decreased, the amount of LDH leakage and the activity of SOD decreased, the content of NO and MDA in the culture fluid increased significantly(P<0.05); Compared with the Sevo group, the viability of HT22 cells in DHA + Sevo group was sig-nificantly increased, the leakage of LDH in the culture fluid decreased, and the activity of SOD increased. The content of NO and MDA in the culture fluid decreased(P<0.05). Conclusion: DHA attenuates sevoflurane-induced damage to HT22 cells through an anti-oxidative stress response.
Objective: To observe whether DHA ameliorate sevoflurane-induced neuronal damage and explore the possible mechanism. Methods: Cells were assigned to Con group, DHA group, Sevo group and DHA+Sevo group. After treated, the cell morphology changes were photographed under inverted phase contrast microscope, and cell suvival was assessed by MTT assay; then the medium was used to assess the concentration of LDH, SOD, NO and MDA. Results: In CON and DHA groups, the cell morphology was normal,the cells in Servo group were collapsed, the cell body ruptured and the normal morphology disappeared. However, the cell morphology in DHA+Sevo group was normal and there were fewer rupture cells. Compared with CON group and DHA group, the survival rate of HT22 cells in Sevo group decreased, the amount of LDH leakage and the activity of SOD decreased, the content of NO and MDA in the culture fluid increased significantly(P<0.05); Compared with the Sevo group, the viability of HT22 cells in DHA + Sevo group was sig-nificantly increased, the leakage of LDH in the culture fluid decreased, and the activity of SOD increased. The content of NO and MDA in the culture fluid decreased(P<0.05). Conclusion: DHA attenuates sevoflurane-induced damage to HT22 cells through an anti-oxidative stress response.
引文
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[2]吴丹,黄俊梅,刘君,等.不同麻醉维持方法对老年患者早期术后认知功能障碍的影响[J].现代生物医学进展,2017,17(11):2093-2098Wu Dan,Huang Jun-mei,Liu Jun,et al.Effect of Different Anesthesia Maintenance Methods on Cognitive Dysfunction in Elderly Patients after Early Operation[J].Progress in Modern Biomedicine,2017,17(11):2093-2098
[3]Shinya Y,Yasushi S,Ryosuke A,et al.Suppression of ERK phosphorylation through oxidative stress is involved in the mechanism underlying sevoflurane-induced toxicity in the developing brain[J].Sci Rep,2016,6:21859
[4]Hartmann T,van Wijk N,Wurtman R J,et al.A nutritional approach to ameliorate altered phospholipid metabolism in Alzheimer's disease[J].J Alzheimers Dis,2014,41(3):715-717
[5]Philip K C,Armen K,Rebecca A M,et al.Docosanexaenoic acid(DHA):a modulator of microglia activity and dendritic apine morphology[J].J Neuroinflammation,2015,12:34
[6]张康秦,梁斌,赵蕊,等.不同浓度七氟烷对小儿麻醉后c Tn I,CRP及补体影响研究[J].现代生物医学进展,2016,16(21):4134-4137Zhang Kang-qin,Liang Bin,Zhao Rui,et al.Effects of different concentrations of sevoflurane on c Tn I,CRP and complement after pediatric anesthesia[J].Progress in Modern Biomedicine,2016,16(21):4134-4137
[7]孙敏,张磊,宋淑玲,等.七氟烷对培养的小鼠小胶质细胞炎症因子表达的影响[J].现代生物医学进展,2015,15(15):2840-2844Sun Min,Zhang Lei,Song Shu-ling,et al.Effect of sevoflurane on the expression of microglia inflammatory cytokines in cultured mice[J].Progress in Modern Biomedicine,2015,15(15):2840-2844
[8]Qiu L,Zhu C,Bodogan T,et al.Acute and Long-Term Effects of Brief Sevoflurane Anesthesia During the Early Postnatal Period in Rats[J].Toxicol Sci,2016,149(1):121-133
[9]Fan D,Li J,Zheng B,et al.Enriched Environment Attenuates Surgery-Induced Impairment of Learning,Memory,and Neurogenesis Possibly by Preserving BDNF Expression[J].Mol Neurobiol,2016,53(1):344-354
[10]Van de Rest O,Wang Y,Barnes L L,et al.APOE epsilon4 and the associations of seafood and long-chain omega-3 fatty acids with cognitive decline[J].Neurology,2016,86(22):2063-2070
[11]Drobish J K,Gan Z S,Cornfeld A D,et al.From the Cover:Volatile Anesthetics Transiently Disrupt Neuronal Development in Neonatal Rats[J].Toxicol Sci,2016,154(2):309-319
[12]Zhao Y,Chen K,Shen X.Environmental Enrichment Attenuated Sevoflurane-Induced Neurotoxicity through the PPAR-gamma Signaling Pathway[J].Biomed Res Int,2015,2015:107149
[13]Gui L,Lei X,Zuo Z.Decrease of glial cell-derived neurotrophic factor contributes to anesthesia-and surgery-induced learning and memory dysfunction in neonatal rats[J].J Mol Med(Berl),2017,95(4):369-379
[14]Tao G,Luo Y,Xue Q,et al.Docosahexaenoic Acid Rescues Synaptogenesis Impairment and Long-Term Memory Deficits Caused by Postnatal Multiple Sevoflurane Exposures[J].Biomed Res Int,20162016:4062579
[15]Moon S Y,de Souto B P,Chupin M,et al.Association between Red Blood Cells Omega-3 Polyunsaturated Fatty Acids and White Matter Hyperintensities:The MAPT Study[J].J Nutr Health Aging,2018,22(1):174-179
[16]Ho C F,Bon C P,Ng Y K,et al.Expression of DHA-Metabolizing Enzyme Alox15 is Regulated by Selective Histone Acetylation in Neuroblastoma Cells[J].Neurochem Res,2017
[17]Hopperton K E,Trepanier M O,James N,et al.Fish oil feeding attenuates neuroinflammatory gene expression without concomitant changes in brain eicosanoids and docosanoids in a mouse model of Alzheimer's disease[J].Brain Behav Immun,2017[Epub ahead of print]
[18]Mazereeuw G,Herrmann N,Andreazza A C,et al.Baseline Oxidative Stress Is Associated with Memory Changes in Omega-3 Fatty Acid Treated Coronary Artery Disease Patients[J].Cardiovasc Psychiatry Neurol,2017,2017:3674371
[19]Valentini K J,Pickens C A,Wiesinger J A,et al.The effect of fish oil supplementation on brain DHA and EPA content and fatty acid profile in mice[J].Int J Food Sci Nutr,2017:1-13
[20]Chuang D Y,Simonyi A,Kotzbauer P T,et al.Cytosolic phospholipase A2 plays a crucial role in ROS/NO signaling during microglial activation through the lipoxygenase pathway[J].J Neuroinflammation,2015,12:199
[21]Francos-Quijorna I,Santos-Nogueira E,Gronert K,et al.Maresin 1Promotes Inflammatory Resolution,Neuroprotection,and Functional Neurological Recovery After Spinal Cord Injury[J].J Neurosci,2017,37(48):11731-11743
[22]Bu J,Dou Y,Tian X,et al.The Role of Omega-3 Polyunsaturated Fatty Acids in Stroke[J].Oxid Med Cell Longev,2016,2016:6906712
[23]Pu H,Guo Y,Zhang W,et al.Omega-3 polyunsaturated fatty acid supplementation improves neurologic recovery and attenu ates white matter injury after experimental traumatic brain injury[J].J Cereb Blood Flow Metab,2013,33(9):1474-1484
[24]Begum G,Harvey L,Dixon C E,et al.ER stress and effects of DHAas an ER stress inhibitor[J].Transl Stroke Res,2013,4(6):635-642
[25]Arunagiri P,Balamurugan E,Saravanakumar M,et al.Omega-3 fatty acids supplementation with lithium and aripiprazole for improving the balance of circulating hormones and brain neurotransmitters in manic mice model[J].Naunyn Schmiedebergs Arch Pharmacol,2017
[26]Avci B,Bilge S S,Arslan G,et al.Protective effects of dietary omega-3 fatty acid supplementation on organophosphate poisoning[J].Toxicol Ind Health,2017:1124833106
[27]Balanza M V.Nutritional supplements in psychotic disorders[J].Actas Esp Psiquiatr,2017,45(Supplement):16-25
[28]Mc Dougle D R,Watson J E,Abdeen A A,et al.Anti-inflammatory omega-3 endocannabinoid epoxides[J].Proc Natl Acad Sci U S A,2017,114(30):E6034-E6043
[29]Trojian T H,Wang D H,Leddy J J.Nutritional Supplements for the Treatment and Prevention of Sports-Related Concussion-Evidence Still Lacking[J].Curr Sports Med Rep,2017,16(4):247-255
[30]Bernier P J,Gourdeau C,Carmichael P H,et al.Validation and diagnostic accuracy of predictive curves for age-associated longitudinal cognitive decline in older adults[J].CMAJ,2017,189(48):E1472-E1480