浸提法与凯氏定氮蒸馏法测定湿米粉中双乙酸钠含量的比较
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摘要
利用直接浸提法和凯氏定氮蒸馏装置,建立湿米粉中双乙酸钠的高效液相色谱法快速检测。湿米粉中的双乙酸钠经过磷酸酸化后转化为酸,通过凯氏定氮蒸馏装置、超声浸提法,用磷酸溶液(1 mol/L)调p H为3.0左右,采用高效液相色谱法进行测定。结果表明,直接浸提法双乙酸钠的回收率为98.32%~103.13%,相对标准偏差为0.322%~0.877%;凯氏定氮蒸馏法双乙酸钠的回收率为98.12%~102.52%,相对标准偏差为0.317%~1.750%,说明凯氏定氮蒸馏法简便快捷、稳定可靠,适用于鲜湿米粉中双乙酸钠含量的测定。
Using Direct extraction and kjeldahl distillation apparatus, a method for the simultaneous determination of sodium diacetate for wet rice noodles by high performance liquid chromatography(HPLC) was established. Methods the sodium diacetate in the wet rice noodles was converted to the corresponding acids after phosphoric acid acidification,After that,acid was collected in the Kjeldahl nitrogen distillation unit. The p H of the distillates was adjusted to around 3.0 by phosphoric acid solution(1 mol/L). Finally, HPLC was used for the determination. Recoveries of direct extraction were between 98.32% ~103.13%,and the RSD was in the range of 0.322%~0.877%; Recoveries of kjeldahl nitrogen distillation were between 98.12%~102.52%,and the RSD was in the range of 0.317%~1.750%(n=10). Conclusion The method of kjeldahl distillation is simple,fast, stable and reliable, and it is suitable for the determination of sodium diacetate content in wet rice noodles.
Using Direct extraction and kjeldahl distillation apparatus, a method for the simultaneous determination of sodium diacetate for wet rice noodles by high performance liquid chromatography(HPLC) was established. Methods the sodium diacetate in the wet rice noodles was converted to the corresponding acids after phosphoric acid acidification,After that,acid was collected in the Kjeldahl nitrogen distillation unit. The p H of the distillates was adjusted to around 3.0 by phosphoric acid solution(1 mol/L). Finally, HPLC was used for the determination. Recoveries of direct extraction were between 98.32% ~103.13%,and the RSD was in the range of 0.322%~0.877%; Recoveries of kjeldahl nitrogen distillation were between 98.12%~102.52%,and the RSD was in the range of 0.317%~1.750%(n=10). Conclusion The method of kjeldahl distillation is simple,fast, stable and reliable, and it is suitable for the determination of sodium diacetate content in wet rice noodles.
引文
[1]郝媛媛.高效液相色谱法同时测定食品中丙酸钙(钠)和双乙酸钠[J].化学分析计量,2016,25(6):2204-2207.
[2]林清荷.高效液相色谱法同时测定奶粉中丙酸钙(钠)、双乙酸钠、苯甲酸、山梨酸、脱氢乙酸[J].福建轻纺,2012,12(12):45-47.
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[4]GB 2760-2014,食品安全国家标准食品添加剂使用标准[S].
[5]GB/T 23383-2009,食品中双乙酸钠的测定高效液相色谱法[S].
[6]黄思瑜,汤晓艳,胡长坤,等.高效液相色谱法测定冷鲜肉表面乳酸、柠檬酸及双乙酸钠[J].江苏农业科学,2012,40(1):251-252.
[7]吴新,梦醒,余雯静,等.高效液相色谱法测定牛奶中双乙酸钠[J].农产品加工(学刊),2012(10):143-145,153.
[8]赵娅鸿.离子色谱法测定食品中防腐剂双乙酸钠的研究[J].现代仪器,2011,17(1):79-80,91.
[9]GB 5009.277-2016,食品安全国家标准食品中双乙酸钠的测定[S].
[10]蒲云月,陈少波.高效液相色谱法测定奶粉中双乙酸钠[J].食品工业,2017,38(1):296-298.
[2]林清荷.高效液相色谱法同时测定奶粉中丙酸钙(钠)、双乙酸钠、苯甲酸、山梨酸、脱氢乙酸[J].福建轻纺,2012,12(12):45-47.
[3]朱洪亮.高效液相色谱法测定食品中双乙酸钠和丙酸盐含量[J].食品安全质量检测学报,2014,5(7):2205-2207.
[4]GB 2760-2014,食品安全国家标准食品添加剂使用标准[S].
[5]GB/T 23383-2009,食品中双乙酸钠的测定高效液相色谱法[S].
[6]黄思瑜,汤晓艳,胡长坤,等.高效液相色谱法测定冷鲜肉表面乳酸、柠檬酸及双乙酸钠[J].江苏农业科学,2012,40(1):251-252.
[7]吴新,梦醒,余雯静,等.高效液相色谱法测定牛奶中双乙酸钠[J].农产品加工(学刊),2012(10):143-145,153.
[8]赵娅鸿.离子色谱法测定食品中防腐剂双乙酸钠的研究[J].现代仪器,2011,17(1):79-80,91.
[9]GB 5009.277-2016,食品安全国家标准食品中双乙酸钠的测定[S].
[10]蒲云月,陈少波.高效液相色谱法测定奶粉中双乙酸钠[J].食品工业,2017,38(1):296-298.