质粒介导的喹诺酮耐药细菌的耐药机制
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摘要
喹诺酮类抗菌药物是一种重要的广谱抗生素,属于化学合成抗菌药物。喹诺酮类抗菌药物,由于其优良的抗菌活性,安全性好,目前已在临床、畜牧业和水产业中广泛使用。耐药质粒的水平转移是喹诺酮耐药在不同细菌间传播的主要原因。本文就质粒介导的喹诺酮耐药机制和不同来源菌株质粒介导的喹诺酮耐药在国内的流行情况进行概述。
Quinolones is an important broad-spectrum antibiotic, which belongs to chemically synthesized antibacterial agents. Quinolones are widely used in clinics, animal husbandry and aquaculture due to their excellent antibacterial activity and safety. Horizontal transfer of drug resistance plasmid is the main cause of quinolone resistance among different bacteria.In this review, the prevalence of quinolone resistant plasmid mediated quinolone resistance in China and the recently study of the plasmid mediated resistance mechanism were discussed.
Quinolones is an important broad-spectrum antibiotic, which belongs to chemically synthesized antibacterial agents. Quinolones are widely used in clinics, animal husbandry and aquaculture due to their excellent antibacterial activity and safety. Horizontal transfer of drug resistance plasmid is the main cause of quinolone resistance among different bacteria.In this review, the prevalence of quinolone resistant plasmid mediated quinolone resistance in China and the recently study of the plasmid mediated resistance mechanism were discussed.
引文
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[3]Martínez-Martínez L,Pascual A,Jacoby GA.Quinolone resistance from a transferable plasmid[J].Lancet,1998,351(9105):797-799.
[4]Hata M,Suzuki M,Matsumoto M,et al.Cloning of a novel gene for quinolone resistance from a transferable plasmid in Shigella flexneri 2b[J].Antimicrob Agents Chemother,2005,49(2):801-803.
[5]Jacoby GA,Walsh KE,Mills DM,et al.qnr B,another plasmid-mediated gene for quinolone resistance[J].Antimicrob Agents Chemother,2006,50(4):1178-1182.
[6]Cavaco LM,Hasman H,Xia S,et al.qnr D,a Novel gene conferring transferable quinolone resistance in salmonella enterica serovar kentucky and bovismorbificans strains of human origin[J].Antimicrob Agents Chemother,2009,53(2):603-608.
[7]Wang M,Guo QX.New plasmid-mediated quinolone resistance gene,qnr C,found in a clinical isolate of Proteus mirabilis[J].Antimicrob Agents Chemother,2009,53(5):1892-1897.
[8]Robicsek A,Strahilevitz J,Jacoby GA,et al.Fluoroquinolonemodifying enzyme:a new adaptation of a common aminoglycoside acetyltransferase[J].Nat Med,2006,12(1):83-88.
[9]Kunikazu Y,Jun-Ichi W,Satowa S,et al.New plasmidmediated fluoroquinolone efflux pump,Qep A,found in an Escherichia coli clinical isolate[J].Antimicrob Agents Chemother,2007,32(1):249-256.
[10]Sorensen AH,Hansen LH,Johannesen E,et al.Conjugative plasmid conferring resistance to olaquindox[J].Antimicrob Agents Chemother,2003,47(2):798-799.
[11]Hansen L H,Johannesen E,Burm?lle M,et al.Plasmidencoded multidrug efflux pump conferring resistance to olaquindox in Escherichia coli[J].Antimicrob Agents Chemother,2004,48(9):3332-3337.
[12]程然,靖杰,伍勇,等.2015年湘雅三医院临床分离病原菌分布的特点及耐药性分析[J].实用预防医学,2017,24(1):109-113.Cheng R,Jing J,Wu Y,et al.Distribution characteristics and drug resistance of clinically isolated pathogenic bacteria in the Thrid Xiangya Hospital,2015[J].Pract Prev Med,2017,24(1):109-113.
[13]林浩,李述剑.大肠埃希菌耐喹诺酮类抗菌药的相关耐药基因检测及耐药机制分析[J].国外医药:抗生素分册,2016,37(6):263-265.Lin H,Li SJ.An analysis of quinolone antibiotic resistance of E.Coli and the plasmid-mediated mechanism[J].World Notes on Antibiotics,2016,37(6):263-265.
[14]曹小利,郑波,李耘,等.质粒介导的喹诺酮耐药基因在中国大陆尿液分离的大肠埃希菌中的广泛分布[J].中国临床药理学杂志,2012,28(2):103-106.Cao XL,Zheng B,Li Y,et al.Broad distribution of plasmid mediated quinolone resistance genes among urinary Escherichia coli in mainland,China[J].Chin J Clin Pharmacol,2012,28(2):103-106.
[15]张增峰,孟晓风,杨保伟,等.鸡肉源沙门氏菌对(氟)喹诺酮类抗生素的耐药性及相关基因[J].中国食品学报,2015,15(3):158-165.Zhang ZF,Meng XF,Yang BW,et al.Drug resistance and related genes of chickenborne Salmonella fluoroquinolones[J].JChin Inst Food Sci Tech,2015,15(3):158-165.
[16]白莉,张秀丽,甘辛,等.肉鸡养殖场中环丙沙星和头孢噻肟双重耐药沙门菌耐药机制的研究[J].中国食品卫生杂志,2015,27(5):487-494.Bai L,Zhang XL,Gan X,et al.Molecular characteristics of ciprofloxacin and cefotaxime co-resistant Salmonella isolates in broiler flocks[J].Chin J Food Hyg,2015,27(5):487-494.
[17]江萍,关茹飞,夏利宁,等.新疆猪源沙门氏菌耐药性及耐药基因检测[J].中国畜牧兽医,2017,44(3):896-903.Jiang P,Guan RF,Xia LN,et al.Detection of resistance and resistance genes of salmonellafrom spine in Xinjiang[J].Chin Anim Husb Vet Med,2017,44(3):896-903.
[18]潘玫,陈山,李稳,等.大肠埃希菌耐药特征及质粒介导喹诺酮耐药基因分布[J].中国感染与化疗杂志,2016,16(1):15-19.Pan M,Chen S,Li W,et al.Resistance profile and prevalence of plasmid-mediated quinolone resistance genes in Escherichia coli isolates[J].Chin J Infect Chemother,2016,16(1):15-19.
[19]姜晓冰,于涛,王青霞.临床分离铜绿假单胞菌质粒介导的喹诺酮耐药机制研究[J].中国抗生素杂志,2015,40(1):61-65.Jiang XB,Yu T,Wang QX.Detection of plasmid-mediated quinolone resistance genesin clinical isolates of Pseudomonas aeruginosa[J].Chin J Antibio,2015,40(1):61-65.
[20]蔡长争,舒少为,陈爱平,等.深圳市菌痢流行分子特征与对质粒介导的喹诺酮耐药机制[J].中华实验和临床感染病杂志:电子版,2016,10(3):285-289.Cai CZ,Shu SW,Chen AP,et al.Mechanisms of plasmid mediated quinolones in Shenzhen[J].Chin J Exp Clin Infect Dis:Electronic Edit,2016,10(3):285-289.
[21]李美,刘宝,万珊,等.阴沟肠杆菌质粒介导耐药基因检测及同源性分析[J].中华医院感染学杂志,2016,26(22):5060-5063.Li M,Liu B,Wan S,et al.Detection of plasmid-mediated resistance genes in Enterobacter cloacae[J].Chin J Nosocomiol,2016,26(22):5060-5063.
[22]Cao J,Li M,Xu C,et al.Characterization of integrons and qnr genes in proteeae from a teaching hospital in China[J].Chemotherapy,2016,62(1):12.
[23]刘建民,张瑞华,姜世金.潍坊市肉鸭源大肠杆菌喹诺酮类药物耐药性的检测[J].家禽科学,2015,(11):17-20.Liu JM,Zhang RH,Jiang SJ,Detection of quinolone resistance in Escherichia coli in Weifang duck source[J].Poultry Sci,2015,(11):17-20.
[24]坤清芳,耿毅,余泽辉,等.兔源大肠杆菌对喹诺酮药物耐药性及质粒介导的耐药基因检测[J].中国预防兽医学报,2016,38(12):944-948.Kun QF,Geng Y,Yu ZH,et al.Detection of quinolone resistance and plasmid mediated quinolone resistance gene in Escherichia coli isolated from rabbit[J].Chin J Prevent Veterin Med,2016,38(12):944-948.
[25]姜晓冰,徐雅梦,于涛,等.鲍曼不动杆菌质粒介导喹诺酮耐药机制研究[J].中国抗生素杂志,2016,41(5):382-387.Jiang XB,Xu YM,Yu T,et al.Detection of plasmid-mediated quinolone resistance genes in Acinetobacter baumannii isolates[J].Chin J Antibio,2016,41(5):382-387.
[26]李梅,王文香,潘发愤,等.铜绿假单胞菌质粒介导的喹诺酮耐药机制研究[J].浙江医学,2016,38(1):29-31.Li M,Wang WX,Pan FF,et al.Identification of multidrug resistance genes in quinolone-resistance Pseudomonas aeruginosa with plasmid conjugation method[J].Zhejiang Med J,2016,38(1):29-31.
[27]茆海丰,刘洪书,赵勇,等.大肠埃希菌对喹诺酮类抗菌药物耐药机制研究[J].中华医院感染学杂志,2015,25(15):3375-3377.Mao HF,Liu HS,Zhao Y,et al.Study on drug resistant mechanisms of Escherichia coli to quinolone[J].Chin JNosocomiol,2015,25(15):3375-3377.
[28]刘贵深,于涛.食源性沙门氏菌耐药性及质粒介导喹诺酮耐药基因检测[J].生物技术通报,2014,46(8):202-207.Liu GS,Yu T.Study on antimicrobial resistance and plasmidmediated quinolone resistance of foodborne salmonella isolates[J].Biotechnol Bullet,2014,46(8):202-207.
[29]姜晓冰,张家洋,王艳博,等.动物性食品源大肠杆菌质粒介导喹诺酮的耐药机制研究[J].现代食品科技,2016,32(1):58-64.Jiang XB,Zhang JY,Wang YB,et al.Mechanism of plasmidmediated quinolone resistance in Escherichia coli isolated from animal-derived food[J].Modern Food Science&Technology,2016,32(1):58-64.
[30]Jiang X,Yu T,Wu N,et al.Detection of qnr,aac(6′)-Ib-cr,and qep A,genes in Escherichia coli,isolated from cooked meat products in Henan,China[J].Int J Food Microbiol,2014,187(187C):22-25.
[31]葛琨,武运,杨保伟,等.乌鲁木齐牛羊肉源沙门氏菌对喹诺酮类药物的耐药状况及相关基因分析(下转第138页)[J].食品科学,2017,38(4):107-112.Ge K,Wu Y,Yang BW,et al.Quinolone resistance characteristics and related gene analysis of Salmonella in beef and mutton retailed inürümqi[J].Food Sci,2017,38(4):107-112.
[32]Zhang Z,Meng X,Wang Y,et al.Presence of qnr,aac(6')-Ib,qep A,oqx AB,and mutations in gyrase and topoisomerase in nalidixic acid-resistant Salmonella isolates recovered from retail chicken carcasses[J].Foodborne Pathog Dis,2014,11(9):698-705.
[33]Wen Y,Pu X,Wei Z,et al.High prevalence of plasmidmediated quinolone resistance and incq plasmids carrying qnr S2gene in bacteria from rivers near hospitals and aquaculture in China[J].Plos One,2016,11(7):e0159418.
[2]Zhang QQ,Ying GG,Pan CG,et al.Comprehensive evaluation of antibiotics emission and fate in the river basins of china:source analysis,multimedia modeling,and linkage to bacterial resistance[J].Environ Sci Technol,2015,49(11):6772-6782.
[3]Martínez-Martínez L,Pascual A,Jacoby GA.Quinolone resistance from a transferable plasmid[J].Lancet,1998,351(9105):797-799.
[4]Hata M,Suzuki M,Matsumoto M,et al.Cloning of a novel gene for quinolone resistance from a transferable plasmid in Shigella flexneri 2b[J].Antimicrob Agents Chemother,2005,49(2):801-803.
[5]Jacoby GA,Walsh KE,Mills DM,et al.qnr B,another plasmid-mediated gene for quinolone resistance[J].Antimicrob Agents Chemother,2006,50(4):1178-1182.
[6]Cavaco LM,Hasman H,Xia S,et al.qnr D,a Novel gene conferring transferable quinolone resistance in salmonella enterica serovar kentucky and bovismorbificans strains of human origin[J].Antimicrob Agents Chemother,2009,53(2):603-608.
[7]Wang M,Guo QX.New plasmid-mediated quinolone resistance gene,qnr C,found in a clinical isolate of Proteus mirabilis[J].Antimicrob Agents Chemother,2009,53(5):1892-1897.
[8]Robicsek A,Strahilevitz J,Jacoby GA,et al.Fluoroquinolonemodifying enzyme:a new adaptation of a common aminoglycoside acetyltransferase[J].Nat Med,2006,12(1):83-88.
[9]Kunikazu Y,Jun-Ichi W,Satowa S,et al.New plasmidmediated fluoroquinolone efflux pump,Qep A,found in an Escherichia coli clinical isolate[J].Antimicrob Agents Chemother,2007,32(1):249-256.
[10]Sorensen AH,Hansen LH,Johannesen E,et al.Conjugative plasmid conferring resistance to olaquindox[J].Antimicrob Agents Chemother,2003,47(2):798-799.
[11]Hansen L H,Johannesen E,Burm?lle M,et al.Plasmidencoded multidrug efflux pump conferring resistance to olaquindox in Escherichia coli[J].Antimicrob Agents Chemother,2004,48(9):3332-3337.
[12]程然,靖杰,伍勇,等.2015年湘雅三医院临床分离病原菌分布的特点及耐药性分析[J].实用预防医学,2017,24(1):109-113.Cheng R,Jing J,Wu Y,et al.Distribution characteristics and drug resistance of clinically isolated pathogenic bacteria in the Thrid Xiangya Hospital,2015[J].Pract Prev Med,2017,24(1):109-113.
[13]林浩,李述剑.大肠埃希菌耐喹诺酮类抗菌药的相关耐药基因检测及耐药机制分析[J].国外医药:抗生素分册,2016,37(6):263-265.Lin H,Li SJ.An analysis of quinolone antibiotic resistance of E.Coli and the plasmid-mediated mechanism[J].World Notes on Antibiotics,2016,37(6):263-265.
[14]曹小利,郑波,李耘,等.质粒介导的喹诺酮耐药基因在中国大陆尿液分离的大肠埃希菌中的广泛分布[J].中国临床药理学杂志,2012,28(2):103-106.Cao XL,Zheng B,Li Y,et al.Broad distribution of plasmid mediated quinolone resistance genes among urinary Escherichia coli in mainland,China[J].Chin J Clin Pharmacol,2012,28(2):103-106.
[15]张增峰,孟晓风,杨保伟,等.鸡肉源沙门氏菌对(氟)喹诺酮类抗生素的耐药性及相关基因[J].中国食品学报,2015,15(3):158-165.Zhang ZF,Meng XF,Yang BW,et al.Drug resistance and related genes of chickenborne Salmonella fluoroquinolones[J].JChin Inst Food Sci Tech,2015,15(3):158-165.
[16]白莉,张秀丽,甘辛,等.肉鸡养殖场中环丙沙星和头孢噻肟双重耐药沙门菌耐药机制的研究[J].中国食品卫生杂志,2015,27(5):487-494.Bai L,Zhang XL,Gan X,et al.Molecular characteristics of ciprofloxacin and cefotaxime co-resistant Salmonella isolates in broiler flocks[J].Chin J Food Hyg,2015,27(5):487-494.
[17]江萍,关茹飞,夏利宁,等.新疆猪源沙门氏菌耐药性及耐药基因检测[J].中国畜牧兽医,2017,44(3):896-903.Jiang P,Guan RF,Xia LN,et al.Detection of resistance and resistance genes of salmonellafrom spine in Xinjiang[J].Chin Anim Husb Vet Med,2017,44(3):896-903.
[18]潘玫,陈山,李稳,等.大肠埃希菌耐药特征及质粒介导喹诺酮耐药基因分布[J].中国感染与化疗杂志,2016,16(1):15-19.Pan M,Chen S,Li W,et al.Resistance profile and prevalence of plasmid-mediated quinolone resistance genes in Escherichia coli isolates[J].Chin J Infect Chemother,2016,16(1):15-19.
[19]姜晓冰,于涛,王青霞.临床分离铜绿假单胞菌质粒介导的喹诺酮耐药机制研究[J].中国抗生素杂志,2015,40(1):61-65.Jiang XB,Yu T,Wang QX.Detection of plasmid-mediated quinolone resistance genesin clinical isolates of Pseudomonas aeruginosa[J].Chin J Antibio,2015,40(1):61-65.
[20]蔡长争,舒少为,陈爱平,等.深圳市菌痢流行分子特征与对质粒介导的喹诺酮耐药机制[J].中华实验和临床感染病杂志:电子版,2016,10(3):285-289.Cai CZ,Shu SW,Chen AP,et al.Mechanisms of plasmid mediated quinolones in Shenzhen[J].Chin J Exp Clin Infect Dis:Electronic Edit,2016,10(3):285-289.
[21]李美,刘宝,万珊,等.阴沟肠杆菌质粒介导耐药基因检测及同源性分析[J].中华医院感染学杂志,2016,26(22):5060-5063.Li M,Liu B,Wan S,et al.Detection of plasmid-mediated resistance genes in Enterobacter cloacae[J].Chin J Nosocomiol,2016,26(22):5060-5063.
[22]Cao J,Li M,Xu C,et al.Characterization of integrons and qnr genes in proteeae from a teaching hospital in China[J].Chemotherapy,2016,62(1):12.
[23]刘建民,张瑞华,姜世金.潍坊市肉鸭源大肠杆菌喹诺酮类药物耐药性的检测[J].家禽科学,2015,(11):17-20.Liu JM,Zhang RH,Jiang SJ,Detection of quinolone resistance in Escherichia coli in Weifang duck source[J].Poultry Sci,2015,(11):17-20.
[24]坤清芳,耿毅,余泽辉,等.兔源大肠杆菌对喹诺酮药物耐药性及质粒介导的耐药基因检测[J].中国预防兽医学报,2016,38(12):944-948.Kun QF,Geng Y,Yu ZH,et al.Detection of quinolone resistance and plasmid mediated quinolone resistance gene in Escherichia coli isolated from rabbit[J].Chin J Prevent Veterin Med,2016,38(12):944-948.
[25]姜晓冰,徐雅梦,于涛,等.鲍曼不动杆菌质粒介导喹诺酮耐药机制研究[J].中国抗生素杂志,2016,41(5):382-387.Jiang XB,Xu YM,Yu T,et al.Detection of plasmid-mediated quinolone resistance genes in Acinetobacter baumannii isolates[J].Chin J Antibio,2016,41(5):382-387.
[26]李梅,王文香,潘发愤,等.铜绿假单胞菌质粒介导的喹诺酮耐药机制研究[J].浙江医学,2016,38(1):29-31.Li M,Wang WX,Pan FF,et al.Identification of multidrug resistance genes in quinolone-resistance Pseudomonas aeruginosa with plasmid conjugation method[J].Zhejiang Med J,2016,38(1):29-31.
[27]茆海丰,刘洪书,赵勇,等.大肠埃希菌对喹诺酮类抗菌药物耐药机制研究[J].中华医院感染学杂志,2015,25(15):3375-3377.Mao HF,Liu HS,Zhao Y,et al.Study on drug resistant mechanisms of Escherichia coli to quinolone[J].Chin JNosocomiol,2015,25(15):3375-3377.
[28]刘贵深,于涛.食源性沙门氏菌耐药性及质粒介导喹诺酮耐药基因检测[J].生物技术通报,2014,46(8):202-207.Liu GS,Yu T.Study on antimicrobial resistance and plasmidmediated quinolone resistance of foodborne salmonella isolates[J].Biotechnol Bullet,2014,46(8):202-207.
[29]姜晓冰,张家洋,王艳博,等.动物性食品源大肠杆菌质粒介导喹诺酮的耐药机制研究[J].现代食品科技,2016,32(1):58-64.Jiang XB,Zhang JY,Wang YB,et al.Mechanism of plasmidmediated quinolone resistance in Escherichia coli isolated from animal-derived food[J].Modern Food Science&Technology,2016,32(1):58-64.
[30]Jiang X,Yu T,Wu N,et al.Detection of qnr,aac(6′)-Ib-cr,and qep A,genes in Escherichia coli,isolated from cooked meat products in Henan,China[J].Int J Food Microbiol,2014,187(187C):22-25.
[31]葛琨,武运,杨保伟,等.乌鲁木齐牛羊肉源沙门氏菌对喹诺酮类药物的耐药状况及相关基因分析(下转第138页)[J].食品科学,2017,38(4):107-112.Ge K,Wu Y,Yang BW,et al.Quinolone resistance characteristics and related gene analysis of Salmonella in beef and mutton retailed inürümqi[J].Food Sci,2017,38(4):107-112.
[32]Zhang Z,Meng X,Wang Y,et al.Presence of qnr,aac(6')-Ib,qep A,oqx AB,and mutations in gyrase and topoisomerase in nalidixic acid-resistant Salmonella isolates recovered from retail chicken carcasses[J].Foodborne Pathog Dis,2014,11(9):698-705.
[33]Wen Y,Pu X,Wei Z,et al.High prevalence of plasmidmediated quinolone resistance and incq plasmids carrying qnr S2gene in bacteria from rivers near hospitals and aquaculture in China[J].Plos One,2016,11(7):e0159418.