红枣中生物碱的提取工艺优化及抗氧化性分析
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摘要
本研究以陕北狗头枣为试验材料,对乙醇回流提取法提取狗头枣生物碱工艺进行优化,并对狗头枣生物碱的抗氧化性进行分析。试验设计主要包括:单因素试验、正交试验设计、抗氧化分析试验。利用单因素试验和正交试验优选最佳提取工艺,再利用生物碱对DPPH自由基的清除作用来测定其抗氧化性。利用乙醇回流提取法提取狗头枣中的生物碱最佳工艺为:料液比为1∶12,乙醇浓度为70%,提取时间为2.5 h;且狗头枣生物碱对DPPH有良好的清除作用。通过对狗头枣生物碱的抗氧化性研究,有利于我们未来对狗头枣的药用价值进行深层次的研究,为广大的种植户增收增产,从而改善他们的生活水平,是将科研与生活紧密联系的最好例证。
A bstract In this study, the G outo u Jujube in North Shaanxi Province was used as experimental material, the ethanol refluxing extraction process of alkaloids from Goutou jujube was optimized, and the anti-oxidation activity of alkaloids from Goutou jujube was analyzed. This experimental design mainly included: single factor experiment,orthogonal experiment design, the anti-oxidation experiment. The single factor test and orthogonal test were used to optimize the best extraction process, and scavenging effect of alkaloids on DPPH free radicals was used to determine its antioxidant activity. The optimum extract ion process of alkaloids from Goutou Jujube by ethanol reflux extraction method was as follows: the material-liquid ratio was 1∶12, the ethanol concentration was 70% and the extraction time was 2.5 h. The alkaloid of Goutou jujube had a strong scavenging effect on DPPH. Through the oxidation resistance study of Goutou jujube alkaloids, it would be helpful for us to make a deep study on the the medicinal value of Goutou Jujube in the future, to increase the production and income for the majority of growers,so as to improve their standard of living, which was the best example of the close relationship between scientific research and life.
A bstract In this study, the G outo u Jujube in North Shaanxi Province was used as experimental material, the ethanol refluxing extraction process of alkaloids from Goutou jujube was optimized, and the anti-oxidation activity of alkaloids from Goutou jujube was analyzed. This experimental design mainly included: single factor experiment,orthogonal experiment design, the anti-oxidation experiment. The single factor test and orthogonal test were used to optimize the best extraction process, and scavenging effect of alkaloids on DPPH free radicals was used to determine its antioxidant activity. The optimum extract ion process of alkaloids from Goutou Jujube by ethanol reflux extraction method was as follows: the material-liquid ratio was 1∶12, the ethanol concentration was 70% and the extraction time was 2.5 h. The alkaloid of Goutou jujube had a strong scavenging effect on DPPH. Through the oxidation resistance study of Goutou jujube alkaloids, it would be helpful for us to make a deep study on the the medicinal value of Goutou Jujube in the future, to increase the production and income for the majority of growers,so as to improve their standard of living, which was the best example of the close relationship between scientific research and life.
引文
Ao D.F.,Ba D.L.H.,and Bu R.E.,2009,Optimizing the extraction process of total alkaloids in Sophora flavescens Ait.by orthogonal experiment,Neimenggu Minzu Daxue Xuebao(Journal of Inner Mongolia University for Nationalities(Na ural Science)),24(1):75-78(敖道夫,巴达拉胡,布日额2009,正交试验法优选苦参总碱提取工艺,内蒙古民族大学学报(自然科学版),24(1):75-78)
Fan J.Y.,and Zhang S.Z.,2010,Technics optimization of total alkaloid extraction from Phellodendron amurense,Jinggangshan Daxue Xuebao(Journal of Jinggangshan University(Natural Science)),31(5):112-117(范家佑,张松竹,2010黄柏中总生物碱的提取工艺优化,井冈山大学学报(自然科学版),31(5):112-117)
Liu Y.Y.,Liang Z.Y.,and Liu P.H.,2009,Optimization of the extraction technology for water soluble alkaloids from Rauwolfia vomitoria Afzel by orthogonal test,Shizhen Guoyi Guoyao(Lishizhen Medicine and Materia Medica Research),20(11):2724-2725(刘洋洋,梁振益,刘平怀,2009,正交实验优选催吐萝芙木水溶性生物碱的提取工艺研究,时珍国医国药,20(11):2724-2725)
Lu J.,Yang F.,and Liu C.M.,2011,Optimization of the extraction technology of total alkanoids from radix Sophorae tonkinensis with ethanol reflux extraction technology,Changchun Shifan Xueyuan Xuebao(Journal of Changchun Normal University(Natural Science)),30(5):48-52(陆娟,杨帆,刘春明,2011,乙醇回流法提取山豆根总生物碱的工艺研究,长春师范学院学报(自然科学版),30(5):48-52)
Shang Y.G.,Li S.F.,and Xiao Y.,2002,Review on extraction technology of alkaloids from plants,Xiandai Huagong(Modern Chemical Industry),22(S):51-59(尚宇光,李淑芬,肖鸯,2002,植物中生物碱的提取工艺,现代化工,22(S):51-59)
Sun J.,Yan T.X.,Tu Y.Q.,Le M.W.,Rao Y.L.,Lei G.,Yan X.W.,and Zhou H.Y.,2015,Orthogonal optimization and verification of RSAP-PCR reaction system in sesame(Sesamum indicum L.),Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(10):2200-2209(孙建,颜廷献,涂玉琴,乐美旺,饶月亮,雷刚,颜小文,周红英,2015,芝麻RSAP-PCR反应体系的正交优化与验证,基因组学与应用生物学,35(10):2200-2209)
Xiao J.,Sun Z.D.,Xie B.J.,and Yang E.N.,2009,Optimization of extraction technology alkaloids in lotus leaf and determination of its scavenging activity of hydroxyl radical,Shipin Gongye Keji(Science and Technology of Food Industry),(7):194-197(肖娟,孙智达,谢笔钧,杨尔宁,2009,荷叶生物碱的提取工艺优化及其清除羟基自由基能力的测定,食品工业科技,(7):194-197)
Zeng J.W.,Xiong X.M.,and Wu J.Z.,2007,Orthogonal text for optimization of process of alkaloids from Plumula neilumbinis,Shizhen Guoyi Guoyao(Lishizhen Medicine and Materia Medica Research),18(7):1615-1617(曾建伟,熊学敏,吴锦忠,2007,正交试验法优选莲子心生物碱的提取工艺,时珍国医国药,18(7):1615-1617)
Zhang D.H.,Huang R.S.,Zuo L.,and Geng Z.H.,2010,Progress on the method of extraction of alkaloids,Zhongguo Yesheng Zhiwu Ziyuan(Chinese Wild Plant Resources),29(5):15-20(张德华,黄仁术,左露,耿朝辉,2010,生物碱的提取方法研究进展,中国野生植物资源,29(5):15-20)
Fan J.Y.,and Zhang S.Z.,2010,Technics optimization of total alkaloid extraction from Phellodendron amurense,Jinggangshan Daxue Xuebao(Journal of Jinggangshan University(Natural Science)),31(5):112-117(范家佑,张松竹,2010黄柏中总生物碱的提取工艺优化,井冈山大学学报(自然科学版),31(5):112-117)
Liu Y.Y.,Liang Z.Y.,and Liu P.H.,2009,Optimization of the extraction technology for water soluble alkaloids from Rauwolfia vomitoria Afzel by orthogonal test,Shizhen Guoyi Guoyao(Lishizhen Medicine and Materia Medica Research),20(11):2724-2725(刘洋洋,梁振益,刘平怀,2009,正交实验优选催吐萝芙木水溶性生物碱的提取工艺研究,时珍国医国药,20(11):2724-2725)
Lu J.,Yang F.,and Liu C.M.,2011,Optimization of the extraction technology of total alkanoids from radix Sophorae tonkinensis with ethanol reflux extraction technology,Changchun Shifan Xueyuan Xuebao(Journal of Changchun Normal University(Natural Science)),30(5):48-52(陆娟,杨帆,刘春明,2011,乙醇回流法提取山豆根总生物碱的工艺研究,长春师范学院学报(自然科学版),30(5):48-52)
Shang Y.G.,Li S.F.,and Xiao Y.,2002,Review on extraction technology of alkaloids from plants,Xiandai Huagong(Modern Chemical Industry),22(S):51-59(尚宇光,李淑芬,肖鸯,2002,植物中生物碱的提取工艺,现代化工,22(S):51-59)
Sun J.,Yan T.X.,Tu Y.Q.,Le M.W.,Rao Y.L.,Lei G.,Yan X.W.,and Zhou H.Y.,2015,Orthogonal optimization and verification of RSAP-PCR reaction system in sesame(Sesamum indicum L.),Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(10):2200-2209(孙建,颜廷献,涂玉琴,乐美旺,饶月亮,雷刚,颜小文,周红英,2015,芝麻RSAP-PCR反应体系的正交优化与验证,基因组学与应用生物学,35(10):2200-2209)
Xiao J.,Sun Z.D.,Xie B.J.,and Yang E.N.,2009,Optimization of extraction technology alkaloids in lotus leaf and determination of its scavenging activity of hydroxyl radical,Shipin Gongye Keji(Science and Technology of Food Industry),(7):194-197(肖娟,孙智达,谢笔钧,杨尔宁,2009,荷叶生物碱的提取工艺优化及其清除羟基自由基能力的测定,食品工业科技,(7):194-197)
Zeng J.W.,Xiong X.M.,and Wu J.Z.,2007,Orthogonal text for optimization of process of alkaloids from Plumula neilumbinis,Shizhen Guoyi Guoyao(Lishizhen Medicine and Materia Medica Research),18(7):1615-1617(曾建伟,熊学敏,吴锦忠,2007,正交试验法优选莲子心生物碱的提取工艺,时珍国医国药,18(7):1615-1617)
Zhang D.H.,Huang R.S.,Zuo L.,and Geng Z.H.,2010,Progress on the method of extraction of alkaloids,Zhongguo Yesheng Zhiwu Ziyuan(Chinese Wild Plant Resources),29(5):15-20(张德华,黄仁术,左露,耿朝辉,2010,生物碱的提取方法研究进展,中国野生植物资源,29(5):15-20)