蔗糖,GA与液体培养基对马铃薯块茎形成的影响
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摘要
马铃薯是全球第四大粮食作物,粮菜兼用,有丰富的营养价值。马铃薯块茎是马铃薯最具经济价值的部分。马铃薯块茎形成的过程受多种调控途径的调控,其中最重要的是蔗糖途径和赤霉素(GA)途径。本实验设计蔗糖浓度,GA浓度和培养基类型3个因素的正交试验,通过对马铃薯的结薯时间,结薯数,马铃薯均重和大薯率的统计发现高浓度的蔗糖能够使马铃薯结薯提前,液体培养基能够增加马铃薯的薯重和大薯率。添加8%蔗糖和0.1 mg/L GA的液体MS培养基能够使马铃薯提前结薯并且得到的马铃薯数量多,薯重大。
Potato is the fourth largest food crop in the world. It has been used for both grain and vegetable and is rich in nutritional value.Potato tubers are the most economically valuable part of the potato. The formation process of potato tuber is regulated by a variety of regulatorypathways,the most important ones of which is the sucrose pathway and the gibberellin(GA)pathway. This experiment was designed toconduct orthogonal testing three factors of sucrose concentration,GA concentration and media type. By the statistics of potato tuber formationtime,the number of potato tubers,potato average weight,and the rate of large potato,it was found that high concentration of sucroseadvanced potato tubering,and liquid medium increased the potato weight and the rate of large potato. The addition of 8% sucrose and 0.1 mg/LGA in liquid MS medium resulted in advanced potato tuber formation as well as more and heavier potatoes.
Potato is the fourth largest food crop in the world. It has been used for both grain and vegetable and is rich in nutritional value.Potato tubers are the most economically valuable part of the potato. The formation process of potato tuber is regulated by a variety of regulatorypathways,the most important ones of which is the sucrose pathway and the gibberellin(GA)pathway. This experiment was designed toconduct orthogonal testing three factors of sucrose concentration,GA concentration and media type. By the statistics of potato tuber formationtime,the number of potato tubers,potato average weight,and the rate of large potato,it was found that high concentration of sucroseadvanced potato tubering,and liquid medium increased the potato weight and the rate of large potato. The addition of 8% sucrose and 0.1 mg/LGA in liquid MS medium resulted in advanced potato tuber formation as well as more and heavier potatoes.
引文
[1]盖钧镒.作物育种各论[M].北京:中国农业出版社,1997:330-352.
[2]梁蕴睦,孟建明,虎岷,等.张掖加工型马铃薯生产中存在的问题及建议[J].甘肃农业科技,2008(12):43-45.
[3]郭利康,高强.天水市马铃薯产业现状及发展对策[J].甘肃农业科技,2008(12):46-48.
[4]何伯伟.如何进一步发展试管薯工程[J].浙江现代农业,1996(6):20-23.
[5]Vander Zaag DE.Recent trends in development,production andutilization of the potato crop in the world[J].Asian Potato Journal,1990,12-19
[6]吕长文,王季春,唐道彬,等.马铃薯试管结薯的光周期诱导效应研究[J].中国马铃薯,2004,18(2):68-72.
[7]王谧,王西瑶,刘帆,等.大量元素不同浓度组合对试管马铃薯结薯的影响[J].中国农学通报,2007,23(2):65-69.
[8]郭得平,应振土,Shan GA.植物激素与马铃薯块茎形成[J].植物生理学报,1991(2):130-133.
[9]Xu X.The role of gibberellin,abscisic acid,and sucrose in theregulation of potato tuber formation in vitro[J].Plant Physiology,1998,117(2):575-584.
[10]杨文玉.不同组织培养条件对马铃薯试管微型薯的诱导[J].马铃薯杂志,1996,10(1):20-22.
[11]黄德宝,唐朝荣.高等植物蔗糖转运的分子调控[J].生物技术通报,2010(4):1-6.
[12]Sheen J,Zhou L,Jang JC.Sugars as signaling molecules[J].Current Opinion in Plant Biology,1999,2(5):410-418.
[13]Raíces M,Ulloa RM,Macintosh GC,et al.St CDPK1 is expressedin potato stolon tips and is induced by high sucrose concentration[J].Journal of Experimental Botany,2003,54(392):2589.
[14]王迎男,秦永林,苏亚拉其其格,樊明寿.外源蔗糖供应对马铃薯块茎形成的影响[C].北京:2015年中国马铃薯大会,2015:339-343.
[15]霍凤兰,栾清业,尹玉花.蔗糖浓度和光照对马铃薯试管薯诱导的影响[J].甘肃农业科技,2009(11):3-5.
[16]Menzel CM.Tuberization in potato at high temperatures:responsesto gibberellin and growth inhibitors[J].Ann Bot,1980,46(3):259-265.
[17]刘梦芸,蒙美莲,门福义,等.光周期对马铃薯块茎形成的影响及对激素的调节[J].中国马铃薯,1994(4):193-197.
[18]全锋,张爱霞,曹先维.植物激素在马铃薯块茎形成发育过程中的作用[J].中国马铃薯,2002(1):29-32.
[19]郭华春,林满.马铃薯块茎诱导中高浓度蔗糖对内源赤霉素活性的影响[C]//张家口:中国作物学会马铃薯专业委员会2002年年会论文集.2002.
[20]Ou Y,Song B,Liu X,et al.Promoter regions of potato vacuolarinvertase gene in response to sugars and hormones[J].PlantPhysiology&Biochemistry,2013,69(8):9.
[21]García MNM,Stritzler M,Capiati DA.Heterologous expression ofArabidopsis ABF4,gene in potato enhances tuberization throughABA-GA crosstalk regulation[J].Planta,2014,239(3):615.
[22]Chincinska IA,Liesche J,Krügel U,et al.Sucrose transporterSt SUT4 from potato affects flowering,tuberization,and shadeavoidance response[J].Plant Physiology,2008,146(2):515.
[23]胡云海,蒋先明.不同糖类和BA对马铃薯(S.Tuberosum)试管薯的影响[J].中国马铃薯,1989(4):203-206.
[24]刘玲玲.光照和培养基类型对马铃薯微型薯诱导结薯的影响[J].黑龙江农业科学,2004(6):21-23.
[25]Yamaguchi S.Gibberellin metabolism and its regulation[J].Annual Review of Plant Biology,2008,59(4):225-251.
[2]梁蕴睦,孟建明,虎岷,等.张掖加工型马铃薯生产中存在的问题及建议[J].甘肃农业科技,2008(12):43-45.
[3]郭利康,高强.天水市马铃薯产业现状及发展对策[J].甘肃农业科技,2008(12):46-48.
[4]何伯伟.如何进一步发展试管薯工程[J].浙江现代农业,1996(6):20-23.
[5]Vander Zaag DE.Recent trends in development,production andutilization of the potato crop in the world[J].Asian Potato Journal,1990,12-19
[6]吕长文,王季春,唐道彬,等.马铃薯试管结薯的光周期诱导效应研究[J].中国马铃薯,2004,18(2):68-72.
[7]王谧,王西瑶,刘帆,等.大量元素不同浓度组合对试管马铃薯结薯的影响[J].中国农学通报,2007,23(2):65-69.
[8]郭得平,应振土,Shan GA.植物激素与马铃薯块茎形成[J].植物生理学报,1991(2):130-133.
[9]Xu X.The role of gibberellin,abscisic acid,and sucrose in theregulation of potato tuber formation in vitro[J].Plant Physiology,1998,117(2):575-584.
[10]杨文玉.不同组织培养条件对马铃薯试管微型薯的诱导[J].马铃薯杂志,1996,10(1):20-22.
[11]黄德宝,唐朝荣.高等植物蔗糖转运的分子调控[J].生物技术通报,2010(4):1-6.
[12]Sheen J,Zhou L,Jang JC.Sugars as signaling molecules[J].Current Opinion in Plant Biology,1999,2(5):410-418.
[13]Raíces M,Ulloa RM,Macintosh GC,et al.St CDPK1 is expressedin potato stolon tips and is induced by high sucrose concentration[J].Journal of Experimental Botany,2003,54(392):2589.
[14]王迎男,秦永林,苏亚拉其其格,樊明寿.外源蔗糖供应对马铃薯块茎形成的影响[C].北京:2015年中国马铃薯大会,2015:339-343.
[15]霍凤兰,栾清业,尹玉花.蔗糖浓度和光照对马铃薯试管薯诱导的影响[J].甘肃农业科技,2009(11):3-5.
[16]Menzel CM.Tuberization in potato at high temperatures:responsesto gibberellin and growth inhibitors[J].Ann Bot,1980,46(3):259-265.
[17]刘梦芸,蒙美莲,门福义,等.光周期对马铃薯块茎形成的影响及对激素的调节[J].中国马铃薯,1994(4):193-197.
[18]全锋,张爱霞,曹先维.植物激素在马铃薯块茎形成发育过程中的作用[J].中国马铃薯,2002(1):29-32.
[19]郭华春,林满.马铃薯块茎诱导中高浓度蔗糖对内源赤霉素活性的影响[C]//张家口:中国作物学会马铃薯专业委员会2002年年会论文集.2002.
[20]Ou Y,Song B,Liu X,et al.Promoter regions of potato vacuolarinvertase gene in response to sugars and hormones[J].PlantPhysiology&Biochemistry,2013,69(8):9.
[21]García MNM,Stritzler M,Capiati DA.Heterologous expression ofArabidopsis ABF4,gene in potato enhances tuberization throughABA-GA crosstalk regulation[J].Planta,2014,239(3):615.
[22]Chincinska IA,Liesche J,Krügel U,et al.Sucrose transporterSt SUT4 from potato affects flowering,tuberization,and shadeavoidance response[J].Plant Physiology,2008,146(2):515.
[23]胡云海,蒋先明.不同糖类和BA对马铃薯(S.Tuberosum)试管薯的影响[J].中国马铃薯,1989(4):203-206.
[24]刘玲玲.光照和培养基类型对马铃薯微型薯诱导结薯的影响[J].黑龙江农业科学,2004(6):21-23.
[25]Yamaguchi S.Gibberellin metabolism and its regulation[J].Annual Review of Plant Biology,2008,59(4):225-251.