DCs及其膜糖蛋白在慢性淋巴细胞性甲状腺炎中的临床研究
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摘要
目的就甲状腺组织树突状细胞(DCs)数量及患者外周血DCs细胞膜糖蛋白变化在慢性淋巴细胞性甲状腺炎(HT)患者病理过程中的意义进行分析研究。方法研究选取确诊HT患者113例,为观察组,同时选取同期健康体检者120例作为对照组;收集患者手术中的甲状腺组织以及外周血进行DCs计数以及DCs膜糖蛋白Galectin-9表达情况进行分析讨论。结果本研究中观察组受试对象113例,对照组120例受试对象基本情况可比性良好。观察组患者分型参照Woolner's分类法,包含L-型(lymphoid type) 39例,O-型(oxyphilic type) 37例,P-型(pronounced type) 37例。L-型HT患者DCs细胞计数为(6. 54±1. 37)×10~6/L,O-型HT患者DCs细胞计数为(9. 55±1. 08)×10~6/L,P-型HT患者DCs细胞计数为(3. 59±1. 33)×10~6/L,全部HT患者的平均DCs细胞计数为(7. 27±1. 35)×10~6/L;均高于对照组的水平(1. 12±0. 34)×10~6/L,差异均有统计学意义(P <0. 05)。使用流式细胞仪检测DCs中Galectin-9蛋白表达比例水平,结果提示L-型HT患者DCs中Galectin-9蛋白表达比例为24. 66%,O-型HT患者DCs中Galectin-9蛋白表达比例为21. 29%,P-型患者为20. 54%,全部患者的平均DCs中Galectin-9蛋白表达比例则为24. 32%;均高于对照组的7. 20%。同时,DCs膜表面糖蛋白Galectin-9表达的Western blot法检测结果也提示观察组显著高于对照组。结论在HT病理过程中存在者DCs细胞的参与,故可见几种常见类型患者DCs细胞计数均高于健康对照组;同时健康人DCs中Galectin-9蛋白量表达高于HT患者水平,可能与HT患者存在过度免疫有关。
Objective To analyze the significance of the number of DCs in thyroid tissue and the changes of DCs in peripheral blood of patients with Hashimoto 's thyroiditis. Methods A total of 113 patients with HT were selected as the observation group and 120 healthy subjects were selected as the control group. The thyroid tissue and the peripheral blood were collected and the expression of Galectin-9 was detected. Results In the study group,113 cases were observed in the observation group and 120 cases in the control group were comparatively good. In the observation group,the patients were classified into 35 cases( lymphoid type),37 cases of O-type( oxyphilic type) and 37 cases of P-type( pronoumced) type( 6. 54 ± 1. 37) × 10~6/L. DCs cells in O-type HT patients were( 9. 55 ± 1. 08) ×10~6/L,DCs in P-type HT patients were( 3. 59 ± 1. 33) × 10~6/L,and the mean of all patients with HT DCs were( 7. 27 ± 1. 35) × 10~6/L,which were higher than those in the control group( 1. 12 ± 0. 34) × 10~6/L. The difference was statistically significant( P < 0. 05). The expression of Galectin-9 protein in DCs was 24. 66% in DCs of L-type HT patients,and the expression of Galectin-9 protein in DCs of O-type HT patients was 21. 29% % And P-type patients were 20. 54%. The proportion of Galectin-9 protein in the mean DCs of all patients was 24. 32%,which was higher than that of the control group( 7. 20%). At the same time,the expression of Galectin-9 in DCs was also significantly higher than that in the control group. Conclusion The number of DCs cells in several common types of patients is higher than that of healthy controls. The expression of galectin-9 protein in healthy human DCs is higher than that in HT patients,which may be related to HT patients Excessive immunity is associated.
Objective To analyze the significance of the number of DCs in thyroid tissue and the changes of DCs in peripheral blood of patients with Hashimoto 's thyroiditis. Methods A total of 113 patients with HT were selected as the observation group and 120 healthy subjects were selected as the control group. The thyroid tissue and the peripheral blood were collected and the expression of Galectin-9 was detected. Results In the study group,113 cases were observed in the observation group and 120 cases in the control group were comparatively good. In the observation group,the patients were classified into 35 cases( lymphoid type),37 cases of O-type( oxyphilic type) and 37 cases of P-type( pronoumced) type( 6. 54 ± 1. 37) × 10~6/L. DCs cells in O-type HT patients were( 9. 55 ± 1. 08) ×10~6/L,DCs in P-type HT patients were( 3. 59 ± 1. 33) × 10~6/L,and the mean of all patients with HT DCs were( 7. 27 ± 1. 35) × 10~6/L,which were higher than those in the control group( 1. 12 ± 0. 34) × 10~6/L. The difference was statistically significant( P < 0. 05). The expression of Galectin-9 protein in DCs was 24. 66% in DCs of L-type HT patients,and the expression of Galectin-9 protein in DCs of O-type HT patients was 21. 29% % And P-type patients were 20. 54%. The proportion of Galectin-9 protein in the mean DCs of all patients was 24. 32%,which was higher than that of the control group( 7. 20%). At the same time,the expression of Galectin-9 in DCs was also significantly higher than that in the control group. Conclusion The number of DCs cells in several common types of patients is higher than that of healthy controls. The expression of galectin-9 protein in healthy human DCs is higher than that in HT patients,which may be related to HT patients Excessive immunity is associated.
引文
1 Borkhuu O,乌云图,金山.桥本氏病患者甲状腺组织受损机制[J].中华内分泌外科杂志,2015,9(1):73-75
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3袁媛,唐晓磊,张林杰.凝集素芯片法分析桥本氏甲状腺炎患者外周血DC细胞膜糖蛋白的表达[J].安徽医科大学学报,2016,51(2):213-217
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5袁媛.Galectin-9~(low)DC在桥本甲状腺炎中作用的研究[D].合肥:安徽医科大学,2015
6王赛,周国华.树突状细胞发育与迁移机制的研究进展[J].临床消化病杂志,2015,20(6):389-391
7蒋应明,万涛,陈国友,等.树突状细胞来源的钙粘着相关蛋白新基因的克隆、表达及功能研究[C].中国免疫学会学术大会会议议程及论文摘要集,2002,4:331-334
8潘开宇.甘露聚糖结合凝集素(MBL)阻遏人巨细胞病毒感染MD-DC细胞的研究[D].宁波:浙江大学,2011
9赵冉,周凤真,冯传强,等.中西医对桥本氏甲状腺炎的研究进展[J].世界最新医学信息文摘:连续型电子期刊,2016,16(8):91-92
10马倩倩,于世鹏,MAQainqain,等.CD4+T淋巴细胞与桥本氏甲状腺炎[J].济宁医学院学报,2015,11(6):434-437
11张晨晨.全国第十三次中西医结合影像学术研讨会全国中西医结合影像学研究进展学习班福建省第八次中西医结合影像学术研讨会论文汇编[C].中国中西医结合学会医学影像专业委员会,2014:7
12黄慧,马瑞婷,闫哲.雌激素在桥本氏甲状腺炎中的作用[J].西部医学,2015,27(10):1441-1444
13李大鹏.碘过量通过诱导Th17细胞分化促进桥本氏甲状腺炎发病机制的研究[D].武汉:华中科技大学,2013
14王平,王建凤,刘善新,等.桥本氏甲状腺炎发病机制及治疗方法研究进展[J].药学研究,2015,17(10):599-603
15易露露.系统性红斑狼疮合并桥本氏甲状腺炎的临床分析[D].昆明:昆明医科大学,2016
16幸世涛,吴燕霞.Galectin-3、CKl9在桥本氏甲状腺炎中的表达及意义[J].中国现代医生,2012,50(29):86-87
2初平,王棽,邰隽,等.甲状腺过氧化物酶表达在桥本氏甲状腺炎诊断价值的Meta分析[J].标记免疫分析与临床,2014,21(1):6-11
3袁媛,唐晓磊,张林杰.凝集素芯片法分析桥本氏甲状腺炎患者外周血DC细胞膜糖蛋白的表达[J].安徽医科大学学报,2016,51(2):213-217
4柳迎昭,彭辉勇,王丽,等.桥本甲状腺炎患者外周血IL-23水平及意义[J].江苏大学学报:医学版,2015(4):331-334
5袁媛.Galectin-9~(low)DC在桥本甲状腺炎中作用的研究[D].合肥:安徽医科大学,2015
6王赛,周国华.树突状细胞发育与迁移机制的研究进展[J].临床消化病杂志,2015,20(6):389-391
7蒋应明,万涛,陈国友,等.树突状细胞来源的钙粘着相关蛋白新基因的克隆、表达及功能研究[C].中国免疫学会学术大会会议议程及论文摘要集,2002,4:331-334
8潘开宇.甘露聚糖结合凝集素(MBL)阻遏人巨细胞病毒感染MD-DC细胞的研究[D].宁波:浙江大学,2011
9赵冉,周凤真,冯传强,等.中西医对桥本氏甲状腺炎的研究进展[J].世界最新医学信息文摘:连续型电子期刊,2016,16(8):91-92
10马倩倩,于世鹏,MAQainqain,等.CD4+T淋巴细胞与桥本氏甲状腺炎[J].济宁医学院学报,2015,11(6):434-437
11张晨晨.全国第十三次中西医结合影像学术研讨会全国中西医结合影像学研究进展学习班福建省第八次中西医结合影像学术研讨会论文汇编[C].中国中西医结合学会医学影像专业委员会,2014:7
12黄慧,马瑞婷,闫哲.雌激素在桥本氏甲状腺炎中的作用[J].西部医学,2015,27(10):1441-1444
13李大鹏.碘过量通过诱导Th17细胞分化促进桥本氏甲状腺炎发病机制的研究[D].武汉:华中科技大学,2013
14王平,王建凤,刘善新,等.桥本氏甲状腺炎发病机制及治疗方法研究进展[J].药学研究,2015,17(10):599-603
15易露露.系统性红斑狼疮合并桥本氏甲状腺炎的临床分析[D].昆明:昆明医科大学,2016
16幸世涛,吴燕霞.Galectin-3、CKl9在桥本氏甲状腺炎中的表达及意义[J].中国现代医生,2012,50(29):86-87