傣族发酵淘米水的初步研究
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摘要
本文对傣族淘米水的发酵菌株及物质基础进行初步研究,为傣族淘米水在日化领域的开发应用提供实验依据。从传统发酵的淘米水中分离发酵菌株,结合菌株形态和分子生物学方法进行菌株鉴定,通过凯氏定氮法检测不同时间发酵淘米水的总氮及非蛋白氮含量,采用高效液相色谱法分析测定淘米水发酵液中的乳酸和乙酸含量。结果表明,从发酵淘米水样品中分离得到1株乳酸菌和1株酵母菌,通过16S rRNA基因序列及26S rRNA序列分析,结合表形特征,初步鉴定两株菌为乳杆菌属菌株和毕赤酵母属菌株。经48 h发酵,与未发酵淘米水相比,总氮含量提高105%,非蛋白氮含量提高77%,乳酸含量提高551%,乙酸含量提高342%。
The fermentation strain and material basis of fermented rice-washing water of Dai were preliminary studied for supporting the development and application of fermented rice-washing water of Dai in the field of daily chemicals. The fermentation strains were isolated from the sample of fermented rice-washing water of Dai in Xishuangbanna and identified by phylogenetic analyses of 16 SrRNA gene and 26 R rRNA gene respectively,combined with phenotypic characteristics.The content of total nitrogen and non-protein nitrogen were detected by Kjeldahl determination and the concentration of lactic acid and acetic acid were detected by HPLC.The results showed that a lactic acid bacteria and a yeast were isolated from the sample of fermented rice-washing water and were primarily identified as Lactobacillus sp.and Pichia sp..Compared with unfermented rice-washing water,the non-protein nitrogen was increased 105%,the protein nitrogen was increased 77%,the lactic acid and acetic acid were increased 551% and 342% respectively after 48 h fermentation.
The fermentation strain and material basis of fermented rice-washing water of Dai were preliminary studied for supporting the development and application of fermented rice-washing water of Dai in the field of daily chemicals. The fermentation strains were isolated from the sample of fermented rice-washing water of Dai in Xishuangbanna and identified by phylogenetic analyses of 16 SrRNA gene and 26 R rRNA gene respectively,combined with phenotypic characteristics.The content of total nitrogen and non-protein nitrogen were detected by Kjeldahl determination and the concentration of lactic acid and acetic acid were detected by HPLC.The results showed that a lactic acid bacteria and a yeast were isolated from the sample of fermented rice-washing water and were primarily identified as Lactobacillus sp.and Pichia sp..Compared with unfermented rice-washing water,the non-protein nitrogen was increased 105%,the protein nitrogen was increased 77%,the lactic acid and acetic acid were increased 551% and 342% respectively after 48 h fermentation.
引文
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[3]Han SW,Chee KM,Cho SJ.Nutritional quality of rice bran protein in comparison to animal and vegetable protein[J].Food Chemistry,2015,172(3):766.
[4]Mendel F.Rice bran,rice bran oils,and rice hulls:composition,food and industrial uses,and bioactivities in humans,animals,and cells[J].Agricultural and Food Chemistry,2013,61(45):10626-10641.
[5]Wang L,Li X,Chen Z.Sulfated modificat ion of the polysaccharides obtained from defatted rice bran and their antitumor activities[J].International Journal of Biological Macromolecules,2009,44(2):211-214.
[6]Park H Y,Lee KW,Choi HD,et al.Rice bran constituents:immunomodulatory and therapeutic activities[J].Food&Function,2017,8(3):935-943.
[7]Liu Q,Cao X,Zhuang X,et al.Rice bran polysaccharides and oligosaccharides modified by Grifola frondosa fermentation:Antioxidant activities and effects on the production of NO[J].Food Chemistry,2017,223:49-53.
[8]Li,Y,Meng S,Shi M,et al.Bioactivity Evaluation of Crude Polysaccharide from Rice Bran Fermented by Preussia Aemulans and the Changes in its Nutritional Contents[J].Journal of Food Biochemistry,2016,40(5):664-672.
[9]朱磊,汪学德,于新国.米糠蛋白的综合研究进展[J].中国油脂,2013,38(2):81-83.
[10]Fujita H,Yamagami T,Ohshima K.Effects of an aceinhibitory agent,katsuobushi oligopeptide,in the spontaneously hypertensive rat and in borderline and mildly hypertensive subjects 1[J].Nutrition Research,2001,21(8):1149-1158.
[11]Chen J W,Sun Q J,Yao H Y.Opioid Antagonist Activities of Enzymatic Hydrolysates from Rice Bran Protein[J].Food Science,2005,26(6):141-145.
[12]曲晓婷.米糠肽及其免疫调节作用的研究[D].广州:华南理工大学,2007.
[13]Jae-Suk C,Min-Hee J,Woi-Sook M,et al.In vivo hair growth-promoting effect of rice bran extract prepared by supercritical carbon dioxide fluid[J].Biological&Pharmaceutical Bulletin,2014,37(1):44-53.
[14]Choi J S,Park J B,Moon W S,et al.Safety and Efficacy of Rice Bran Supercritical CO2Extract for Hair Growth in Androgenic Alopecia:A 16-Week Double-Blind Randomized Controlled Trial[J].Biological&Pharmaceutical Bulletin,2015,38(12):1856-1863.
[15]陈钧辉.生物化学实验(第四版)[M].北京:科学出版社,2008:59-62.
[16]Pierard-Franchimont C,Pieraed G E,Hair weathering and hair capacitance mapping:a pilot study[J].Journal of Cosmetic Dermatology,2012,11(3):179-182.