淘米水发酵物中2种有机酸的测定
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摘要
采用高效液相色谱法分析测定淘米水发酵液中的有机酸,确定了淘米水发酵液中有机酸分析测定的色谱条件,采用Agilent Zorbax SB-Aq(4.6 mm×150 mm,5μm)色谱柱,流动相为1%甲醇-99%NaH_2PO_4溶液(20 mmol/L,pH=2.0);紫外检测波长210 nm;流速0.4 mL/min;柱温25℃.结果表明,在该条件下淘米水发酵液中的有机酸得到了较好分离,分离检测出乳酸、乙酸2种有机酸.该分析方法简单准确,适用于淘米水发酵液中有机酸的测定分析.
A HPLC(high performance liquid chromatography) method was developed for the determination of organic acids in fermented rice-washing water. HPLC separation was carried out in a Zorbax SB-Aq column(4.6 mm×150 mm,5 μm) with methanol-20 mmol/L NaH_2PO_4 solution(1∶99, V/V) as the mobile phase. The velocity was 0.4 mL/min, the column temperature was 25 ℃, and the detection wave length was 210 nm. The results showed that the lactic acid and acetic acid in the fermented rice-washing water were separated and determined. This simple and accurate method could be used for the determination of organic acids in the fermented rice-washing water.
A HPLC(high performance liquid chromatography) method was developed for the determination of organic acids in fermented rice-washing water. HPLC separation was carried out in a Zorbax SB-Aq column(4.6 mm×150 mm,5 μm) with methanol-20 mmol/L NaH_2PO_4 solution(1∶99, V/V) as the mobile phase. The velocity was 0.4 mL/min, the column temperature was 25 ℃, and the detection wave length was 210 nm. The results showed that the lactic acid and acetic acid in the fermented rice-washing water were separated and determined. This simple and accurate method could be used for the determination of organic acids in the fermented rice-washing water.
引文
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[3] 李喜红, 代红丽, 魏安池. 酶法从脱脂米糠中提取蛋白质[J]. 粮油加工与食品机械, 2005(6):62-64.
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[6] CHOI J S, PARK J B, MOON W S, et al. Safety and efficacy of rice bran supercritical CO2 extract for hair growth in androgenic alopecia:a 16-week double-blind randomized controlled trial[J]. Biological and Pharmaceutical Bulletin, 2015, 38(12):1856-1863.
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[10] 庞荣丽,成昕,方金豹,等. 水果中主要有机酸的离子排斥色谱法测定条件研究[J]. 食品工业, 2015,36(4):261-266.
[11] 向丽娟, 罗忠富. 离子排斥色谱法分离分析若干有机酸[J]. 分析测试学报, 1992(3):41-44.
[12] ALTRIA K D. Analysis of small organic acids by capillary electrophoresis[J]. LC-GC Europe, 2012, 25(12):682-684.
[13] 马瑞,欧阳嘉,李鑫,等. 高效液相色谱法同时测定生物质乳酸发酵液中有机酸及糖类化合物[J]. 色谱, 2012, 30(1):62-66.
[14] 孔维松,宋春满,杨叶昆,等.超高效液相色谱法同时测定烟草中非挥发有机酸[J].云南民族大学学报(自然科学版),2018,27(06):455-459.
[15] 余永建, 邓晓阳,陆震鸣,等. 高效液相色谱法定量分析固态发酵食醋中有机酸的方法优化[J]. 食品科学, 2014, 35(4):55-59.
[2] 周显青, 夏稳稳, 张玉荣. 我国糯米粉加工及其质量控制技术现状与展望[J].粮油食品科技, 2013, 21(3): 1-6.
[3] 李喜红, 代红丽, 魏安池. 酶法从脱脂米糠中提取蛋白质[J]. 粮油加工与食品机械, 2005(6):62-64.
[4] 朱磊, 汪学德, 于新国. 米糠蛋白的综合研究进展[J]. 中国油脂, 2013, 38(2):81-83.
[5] 俞明伟, 张名位, 孙远明,等. 米糠蛋白及其活性肽的研究与利用进展[J]. 中国粮油学报, 2009, 24(5):154-159.
[6] CHOI J S, PARK J B, MOON W S, et al. Safety and efficacy of rice bran supercritical CO2 extract for hair growth in androgenic alopecia:a 16-week double-blind randomized controlled trial[J]. Biological and Pharmaceutical Bulletin, 2015, 38(12):1856-1863.
[7] 可畅. 孔雀公主刀美兰的养发经[J]. 新天地, 2009(3):50.
[8] 陈兴杰,吴攀攀,徐敏锐,等.窖泥己酸菌和丁酸菌联合接种发酵液有机酸检测与分析[J]. 酿酒,2017, 44(3):60-64.
[9] 黄海兰,王鑫,法芸,等.离子色谱法测定蓝藻培养液中有机酸和无机阴离子[J]. 分析科学学报, 2017,33(1):32-36.
[10] 庞荣丽,成昕,方金豹,等. 水果中主要有机酸的离子排斥色谱法测定条件研究[J]. 食品工业, 2015,36(4):261-266.
[11] 向丽娟, 罗忠富. 离子排斥色谱法分离分析若干有机酸[J]. 分析测试学报, 1992(3):41-44.
[12] ALTRIA K D. Analysis of small organic acids by capillary electrophoresis[J]. LC-GC Europe, 2012, 25(12):682-684.
[13] 马瑞,欧阳嘉,李鑫,等. 高效液相色谱法同时测定生物质乳酸发酵液中有机酸及糖类化合物[J]. 色谱, 2012, 30(1):62-66.
[14] 孔维松,宋春满,杨叶昆,等.超高效液相色谱法同时测定烟草中非挥发有机酸[J].云南民族大学学报(自然科学版),2018,27(06):455-459.
[15] 余永建, 邓晓阳,陆震鸣,等. 高效液相色谱法定量分析固态发酵食醋中有机酸的方法优化[J]. 食品科学, 2014, 35(4):55-59.