群体感应系统调控乳酸菌细菌素合成的研究进展
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摘要
乳酸菌细菌素具有广谱的抑菌活性,但其合成量低,限制了行业应用。群体感应是细胞间的通信过程,细菌通过感知信号分子浓度变化,调控相关的生物学过程。三组分系统在调控细菌素合成的过程中发挥了核心作用。本文中,笔者综述了调控乳酸菌细菌素合成的三组分系统的组成、Ⅰ类和Ⅱ类细菌素合成调控的基因与结构的差异性以及细菌密度和共培养等因素对细菌素的合成的影响,以阐明乳酸菌素合成的群体感应调控机制,对于其在食品、生物和医疗领域的应用都有重要的意义。
Lactic acid bacteria bacteriocin has a broad spectrum of antibacterial activity,but its synthesis is low,which limits the industrial application.Quorum sensing is a cell-to-cell communication process in which bacteria regulate related biological processes by sensing changes in signal molecule concentrations.The three-component system plays a key role in the regulation of bacteriocin synthesis.This paper reviews the composition of a three-component system that regulates the synthesis of bacteriocins in lactic acid bacteria,the differences of genes and structures in the regulation of the synthesis for class Ⅰ and class Ⅱ bacteriocins,and the effects of bacterial density and co-culture on the synthesis of bacteriocin.It is of great significance to elucidate the quorum sensing regulation mechanism of lactobacilli synthesis for its application in food,biology and medicine.
Lactic acid bacteria bacteriocin has a broad spectrum of antibacterial activity,but its synthesis is low,which limits the industrial application.Quorum sensing is a cell-to-cell communication process in which bacteria regulate related biological processes by sensing changes in signal molecule concentrations.The three-component system plays a key role in the regulation of bacteriocin synthesis.This paper reviews the composition of a three-component system that regulates the synthesis of bacteriocins in lactic acid bacteria,the differences of genes and structures in the regulation of the synthesis for class Ⅰ and class Ⅱ bacteriocins,and the effects of bacterial density and co-culture on the synthesis of bacteriocin.It is of great significance to elucidate the quorum sensing regulation mechanism of lactobacilli synthesis for its application in food,biology and medicine.
引文
[1] GHANBARI M,JAMI M,DOMIG K J,et al.Seafood biopreservation by lactic acid bacteria:a review[J].LWT-Food Sci Technol,2013,54(2):315-324.
[2] 唐晓婷.植物乳杆菌素的分离纯化及抑菌机制与合成调控基因分析[D].哈尔滨:哈尔滨工业大学,2016.
[3] ZACHAROF M P,LOVITT R W.Bacteriocins produced by lactic acid bacteria:a review[J].APCBEE Procedia,2012(2):50-56.
[4] ROSS R P,MORGAN S,HILL C.Preservation and fermentation:past,present and future[J].Int J Food Microbiol,2002,79:3-16.
[5] SALMOND G,BYCROFT B W,STEWART G,et al.The bacterial enigma-cracking the code of cell-cell communication[J].Mol Microbiol,1995,16(4):615-624.
[6] PAPENFORT K,BASSLER B L.Quorum sensing signal-response systems in gram-negative bacteria[J].Nat Rev Microbiol,2016,14(9):576-588.
[7] KIYMACI M E,ALTANLAR N,GUMUSTAS M,et al.Quorum sensing signals and related virulence inhibition of Pseudomonas aeruginosa by a potential probiotic strain′s organic acid[J].Microb Pathog,2018,121:190-197.
[8] 陆兆新,孟凡强.植物乳杆菌素合成及其调控机制的研究进展[J].南京农业大学学报,2018(5):784-792.
[9] TURAN N B,CHORMEY D S,BUYUKPINAR C,et al.Quorum sensing:little talks for an effective bacterial coordination[J].Trend Anal Chem,2017,91:1-11.
[10] MALDONADO A,JIMENEZ-DIAZ R,RUIZ-BARBA J L.Induction of plantaricin production in Lactobacillus plantarum NC8 after coculture with specific gram-positive bacteria is mediated by an autoinduction mechanism[J].J Bacteriol,2004,186(5):1556-1564.
[11] WRIGHT G S A,SAEKI A,HIKIMA T,et al.Architecture of the complete oxygen-sensing FixL-FixJ two-component signal transduction system[J].Sci Signal,2018,doi:10.1126/scisignal.aaq0825.
[12] GREBE T W,STOCK J B.The histidine protein kinase superfamily[M].Adv Microb Physiol,1999,41:139-227.
[13] MALDONADO-BARRAGáN A,CABALLERO-GUERRERO B,LUCENA-PADROS H,et al.Induction of bacteriocin production by coculture is widespread among plantaricin-producing Lactobacillus plantarum strains with different regulatory operons[J].Food Microbiol,2013,33(1):40-47.
[14] BRURBERG M B,NES I F,EIJSINK V.Pheromone-induced production of antimicrobial peptides in Lactobacillus[J].Mol Microbiol,1997,26(2):347-360.
[15] SYVITSKI R T,TIAN X,SAMPARA K,et al.Structure-activity analysis of quorum-sensing signaling peptides from Streptococcus mutans[J].J Bacteriol,2007,189(4):1441-1450.
[16] SINGH A R.Regulation of Streptomyces chitinases by two-component signal transduction systems and their post translational modifications:a review[J].J Pure Appl Microbiol,2018,12(3):1417-1433.
[17] ZUNIGA M,LUNA GOMEZ-ESCOIN C,GONZALEZ-CANDELAS F.Evolutionary history of the OmpR/IIIA family of signal transduction two component systems in Lactobacillaceae and Leuconostocaceae[J].BMC Evol Biol,2011,11:34.
[18] MONEDERO V,REVILLA-GUARINOS A,ZUNIGA M.Physiological role of two-component signal transduction systems in food-associated lactic acid bacteria[J].Adv Appl Microbiol,2017,99:1-51.
[19] KLEEREBEZEM M.Quorum sensing control of lantibiotic production:nisin and subtilin autoregulate their own biosynthesis[J].Peptides,2004,25(9):1405-1414.
[20] MARTíNEZ B,RODRíGUEZ A,SUáREZ E.Antimicrobial peptides produced by bacteria:the bacteriocins[M]//VILLA T G,VINAS M.New weapons to control bacterial growth.Cham:Springer International Publishing,2016:15-38.
[21] 张香美,李平兰.环境因素对PlnA诱导类植物乳杆菌产生细菌素效果的影响[J].微生物学通报,2013,40(9):1624-1630.
[22] ZHANG X M,SHANG N,ZHANG X,et al.Role of plnB gene in the regulation of bacteriocin production in Lactobacills paraplantarum L-XM1[J].Microbiol Res,2013,168(5):305-310.
[23] NISSEN-MEYER J,OPPEGARD C,ROGNE P,et al.The two-peptide(Class-IIb)bacteriocins:genetics,biosynthesis,structure,and mode of action[M]//DRIDER D,REBUFAT S.Prokaryotic antimicrobial peptides.New York:Springer,2011:197-212.
[24] KLEEREBEZEM M,BOEKHORST J,VAN KRANENBURG R,et al.Complete genome sequence of Lactobacillus plantarum WCFS1[J].Proc Natl Acad Sci USA,2003,100(4):1990-1995.
[25] NAVARRO L,ROJO-BEZARES B,SAENZ Y,et al.Comparative study of the pln locus of the quorum-sensing regulated bacteriocin-producing L.plantarum J51 strain[J].Int J Food Microbiol,2008,128(2):390-394.
[26] MALDONADO A,RUIZ-BARBA J L,JIMENEZ-DIAZ R.Purification and genetic characterization of plantaricin NC8,a novel coculture-inducible two-peptide bacteriocin from Lactobacillus plantarum NC8[J].Appl Environ Microbiol,2003,69(1):383-389.
[27] ROJO-BEZARES B,SAENZ Y,NAVARRO L,et al.Characterization of a new organization of the plantaricin locus in the inducible bacteriocin-producing Lactobacillus plantarum J23 of grape must origin[J].Arch Microbiol,2008,189(5):491-499.
[28] TAI H,FOO H,RAHA A R,et al.Molecular characterisation of new organisation of plnEF and plw loci of bacteriocin genes harbour concomitantly in Lactobacillus plantarum I-UL4[J].Microb Cell Fact,2015,14:89.
[29] DIEP D B,STRAUME D,KJOS M,et al.An overview of the mosaic bacteriocin pln loci from Lactobacillus plantarum[J].Peptides,2009,30(8):1562-1574.
[30] NIKOLSKAYA A N,GALPERIN M Y.A novel type of conserved DNA-binding domain in the transcriptional regulators of the AlgR/AgrA/LytR family[J].Nucleic Acid Res,2002,30(11):2453-2459.
[31] JOHNSBORG O,DIEP D B,NES I F.Structural analysis of the peptide pheromone receptor PlnB,a histidine protein kinase from Lactobacillus plantarum[J].J Bacteriol,2003,185(23):6913-6920.
[32] DRIDER D,FIMLAND G,HECHARD Y,et al.The continuing story of class IIa bacteriocins[J].Microbiol Mol Biol Rev,2006,70(2):564-582.
[33] MALDONADO-BARRAGAN A,CABALLERO-GUERRERO B,MARTIN V,et al.Purification and genetic characterization of gassericin E,a novel co-culture inducible bacteriocin from Lactobacillus gasseri EV1461 isolated from the vagina of a healthy woman[J].BMC Microbiol,2016,16:37.
[34] 刘国荣,任桂美,李雪,等.动物双歧杆菌细菌素bifidocin A群体感应合成调控行为分析[J].食品科学,2018(12):161-166.
[35] MALDONADO-BARRAGAN A,LUIS RUIZ-BARBA J,JIMENEZ-DIAZ R.Knockout of three-component regulatory systems reveals that the apparently constitutive plantaricin-production phenotype shown by Lactobacillus plantarum on solid medium is regulated via quorum sensing[J].Int J Food Microbiol,2009,130(1):35-42.
[36] ROJO-BEZARES B,SAENZ Y,NAVARRO L,et al.Coculture-inducible bacteriocin activity of Lactobacillus plantarum strain J23 isolated from grape must[J].Food Microbiol,2007,24(5):482-491.
[37] SVETOCH E A,ERUSLANOV B V,PERELYGIN V V,et al.Inducer bacteria,unique signal peptides,and low-nutrient media stimulate in vitro bacteriocin production by Lactobacillus spp.and Enterococcus spp.strains[J].J Agric Food Chem,2010,58(10):6033-6038.
[38] TABASCO R,GARCIA-CAYUELA T,PELAEZ C,et al.Lactobacillus acidophilus La-5 increases lactacin B production when it senses live target bacteria[J].Int J Food Microbiol,2009,132(2/3):109-116.
[39] MALDONADO A,RUIZ-BARBA J L,JIMENEZ-DIAZ R.Production of plantaricin NC8 by Lactobacillus plantarum NC8 is induced in the presence of different types of gram-positive bacteria[J].Arch Microbiol,2004,181(1):8-16.
[40] CHANOS P,MYGIND T.Co-culture-inducible bacteriocin production in lactic acid bacteria[J].Appl Microbiol Biotechnol,2016,100(10):4297-4308.
[41] ZHAO J,QUAN C,JIN L,et al.Production,detection and application perspectives of quorum sensing autoinducer-2 in bacteria[J].J Biotechnol,2018,268:53-60.
[42] JIA F,PANG X,ZHU D,et al.Role of the luxS gene in bacteriocin biosynthesis by Lactobacillus plantarum KLDS1.0391:a proteomic analysis[J].Sci Rep,2017,7:13871.
[43] DI CAGNO R,DE ANGELIS M,CALASSO M,et al.Quorum sensing in sourdough Lactobacillus plantarum DC400:induction of plantaricin A(PlnA)under co-cultivation with other lactic acid bacteria and effect of PlnA on bacterial and Caco-2 cells[J].Proteomics,2010,10(11):2175-2190.
[2] 唐晓婷.植物乳杆菌素的分离纯化及抑菌机制与合成调控基因分析[D].哈尔滨:哈尔滨工业大学,2016.
[3] ZACHAROF M P,LOVITT R W.Bacteriocins produced by lactic acid bacteria:a review[J].APCBEE Procedia,2012(2):50-56.
[4] ROSS R P,MORGAN S,HILL C.Preservation and fermentation:past,present and future[J].Int J Food Microbiol,2002,79:3-16.
[5] SALMOND G,BYCROFT B W,STEWART G,et al.The bacterial enigma-cracking the code of cell-cell communication[J].Mol Microbiol,1995,16(4):615-624.
[6] PAPENFORT K,BASSLER B L.Quorum sensing signal-response systems in gram-negative bacteria[J].Nat Rev Microbiol,2016,14(9):576-588.
[7] KIYMACI M E,ALTANLAR N,GUMUSTAS M,et al.Quorum sensing signals and related virulence inhibition of Pseudomonas aeruginosa by a potential probiotic strain′s organic acid[J].Microb Pathog,2018,121:190-197.
[8] 陆兆新,孟凡强.植物乳杆菌素合成及其调控机制的研究进展[J].南京农业大学学报,2018(5):784-792.
[9] TURAN N B,CHORMEY D S,BUYUKPINAR C,et al.Quorum sensing:little talks for an effective bacterial coordination[J].Trend Anal Chem,2017,91:1-11.
[10] MALDONADO A,JIMENEZ-DIAZ R,RUIZ-BARBA J L.Induction of plantaricin production in Lactobacillus plantarum NC8 after coculture with specific gram-positive bacteria is mediated by an autoinduction mechanism[J].J Bacteriol,2004,186(5):1556-1564.
[11] WRIGHT G S A,SAEKI A,HIKIMA T,et al.Architecture of the complete oxygen-sensing FixL-FixJ two-component signal transduction system[J].Sci Signal,2018,doi:10.1126/scisignal.aaq0825.
[12] GREBE T W,STOCK J B.The histidine protein kinase superfamily[M].Adv Microb Physiol,1999,41:139-227.
[13] MALDONADO-BARRAGáN A,CABALLERO-GUERRERO B,LUCENA-PADROS H,et al.Induction of bacteriocin production by coculture is widespread among plantaricin-producing Lactobacillus plantarum strains with different regulatory operons[J].Food Microbiol,2013,33(1):40-47.
[14] BRURBERG M B,NES I F,EIJSINK V.Pheromone-induced production of antimicrobial peptides in Lactobacillus[J].Mol Microbiol,1997,26(2):347-360.
[15] SYVITSKI R T,TIAN X,SAMPARA K,et al.Structure-activity analysis of quorum-sensing signaling peptides from Streptococcus mutans[J].J Bacteriol,2007,189(4):1441-1450.
[16] SINGH A R.Regulation of Streptomyces chitinases by two-component signal transduction systems and their post translational modifications:a review[J].J Pure Appl Microbiol,2018,12(3):1417-1433.
[17] ZUNIGA M,LUNA GOMEZ-ESCOIN C,GONZALEZ-CANDELAS F.Evolutionary history of the OmpR/IIIA family of signal transduction two component systems in Lactobacillaceae and Leuconostocaceae[J].BMC Evol Biol,2011,11:34.
[18] MONEDERO V,REVILLA-GUARINOS A,ZUNIGA M.Physiological role of two-component signal transduction systems in food-associated lactic acid bacteria[J].Adv Appl Microbiol,2017,99:1-51.
[19] KLEEREBEZEM M.Quorum sensing control of lantibiotic production:nisin and subtilin autoregulate their own biosynthesis[J].Peptides,2004,25(9):1405-1414.
[20] MARTíNEZ B,RODRíGUEZ A,SUáREZ E.Antimicrobial peptides produced by bacteria:the bacteriocins[M]//VILLA T G,VINAS M.New weapons to control bacterial growth.Cham:Springer International Publishing,2016:15-38.
[21] 张香美,李平兰.环境因素对PlnA诱导类植物乳杆菌产生细菌素效果的影响[J].微生物学通报,2013,40(9):1624-1630.
[22] ZHANG X M,SHANG N,ZHANG X,et al.Role of plnB gene in the regulation of bacteriocin production in Lactobacills paraplantarum L-XM1[J].Microbiol Res,2013,168(5):305-310.
[23] NISSEN-MEYER J,OPPEGARD C,ROGNE P,et al.The two-peptide(Class-IIb)bacteriocins:genetics,biosynthesis,structure,and mode of action[M]//DRIDER D,REBUFAT S.Prokaryotic antimicrobial peptides.New York:Springer,2011:197-212.
[24] KLEEREBEZEM M,BOEKHORST J,VAN KRANENBURG R,et al.Complete genome sequence of Lactobacillus plantarum WCFS1[J].Proc Natl Acad Sci USA,2003,100(4):1990-1995.
[25] NAVARRO L,ROJO-BEZARES B,SAENZ Y,et al.Comparative study of the pln locus of the quorum-sensing regulated bacteriocin-producing L.plantarum J51 strain[J].Int J Food Microbiol,2008,128(2):390-394.
[26] MALDONADO A,RUIZ-BARBA J L,JIMENEZ-DIAZ R.Purification and genetic characterization of plantaricin NC8,a novel coculture-inducible two-peptide bacteriocin from Lactobacillus plantarum NC8[J].Appl Environ Microbiol,2003,69(1):383-389.
[27] ROJO-BEZARES B,SAENZ Y,NAVARRO L,et al.Characterization of a new organization of the plantaricin locus in the inducible bacteriocin-producing Lactobacillus plantarum J23 of grape must origin[J].Arch Microbiol,2008,189(5):491-499.
[28] TAI H,FOO H,RAHA A R,et al.Molecular characterisation of new organisation of plnEF and plw loci of bacteriocin genes harbour concomitantly in Lactobacillus plantarum I-UL4[J].Microb Cell Fact,2015,14:89.
[29] DIEP D B,STRAUME D,KJOS M,et al.An overview of the mosaic bacteriocin pln loci from Lactobacillus plantarum[J].Peptides,2009,30(8):1562-1574.
[30] NIKOLSKAYA A N,GALPERIN M Y.A novel type of conserved DNA-binding domain in the transcriptional regulators of the AlgR/AgrA/LytR family[J].Nucleic Acid Res,2002,30(11):2453-2459.
[31] JOHNSBORG O,DIEP D B,NES I F.Structural analysis of the peptide pheromone receptor PlnB,a histidine protein kinase from Lactobacillus plantarum[J].J Bacteriol,2003,185(23):6913-6920.
[32] DRIDER D,FIMLAND G,HECHARD Y,et al.The continuing story of class IIa bacteriocins[J].Microbiol Mol Biol Rev,2006,70(2):564-582.
[33] MALDONADO-BARRAGAN A,CABALLERO-GUERRERO B,MARTIN V,et al.Purification and genetic characterization of gassericin E,a novel co-culture inducible bacteriocin from Lactobacillus gasseri EV1461 isolated from the vagina of a healthy woman[J].BMC Microbiol,2016,16:37.
[34] 刘国荣,任桂美,李雪,等.动物双歧杆菌细菌素bifidocin A群体感应合成调控行为分析[J].食品科学,2018(12):161-166.
[35] MALDONADO-BARRAGAN A,LUIS RUIZ-BARBA J,JIMENEZ-DIAZ R.Knockout of three-component regulatory systems reveals that the apparently constitutive plantaricin-production phenotype shown by Lactobacillus plantarum on solid medium is regulated via quorum sensing[J].Int J Food Microbiol,2009,130(1):35-42.
[36] ROJO-BEZARES B,SAENZ Y,NAVARRO L,et al.Coculture-inducible bacteriocin activity of Lactobacillus plantarum strain J23 isolated from grape must[J].Food Microbiol,2007,24(5):482-491.
[37] SVETOCH E A,ERUSLANOV B V,PERELYGIN V V,et al.Inducer bacteria,unique signal peptides,and low-nutrient media stimulate in vitro bacteriocin production by Lactobacillus spp.and Enterococcus spp.strains[J].J Agric Food Chem,2010,58(10):6033-6038.
[38] TABASCO R,GARCIA-CAYUELA T,PELAEZ C,et al.Lactobacillus acidophilus La-5 increases lactacin B production when it senses live target bacteria[J].Int J Food Microbiol,2009,132(2/3):109-116.
[39] MALDONADO A,RUIZ-BARBA J L,JIMENEZ-DIAZ R.Production of plantaricin NC8 by Lactobacillus plantarum NC8 is induced in the presence of different types of gram-positive bacteria[J].Arch Microbiol,2004,181(1):8-16.
[40] CHANOS P,MYGIND T.Co-culture-inducible bacteriocin production in lactic acid bacteria[J].Appl Microbiol Biotechnol,2016,100(10):4297-4308.
[41] ZHAO J,QUAN C,JIN L,et al.Production,detection and application perspectives of quorum sensing autoinducer-2 in bacteria[J].J Biotechnol,2018,268:53-60.
[42] JIA F,PANG X,ZHU D,et al.Role of the luxS gene in bacteriocin biosynthesis by Lactobacillus plantarum KLDS1.0391:a proteomic analysis[J].Sci Rep,2017,7:13871.
[43] DI CAGNO R,DE ANGELIS M,CALASSO M,et al.Quorum sensing in sourdough Lactobacillus plantarum DC400:induction of plantaricin A(PlnA)under co-cultivation with other lactic acid bacteria and effect of PlnA on bacterial and Caco-2 cells[J].Proteomics,2010,10(11):2175-2190.