“深层海水”联合热疗对人胃癌SGC-7901细胞活力和凋亡的影响
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摘要
为探讨生理性深层海水(PDSW)联合热疗对人胃癌SGC-7901细胞活力和凋亡的影响,通过培养人胃癌SGC-7901细胞株,随机分为实验组、对照组和空白组,实验组加PDSW,对照组加生理盐水,空白组不做其他处理,分别在37℃、40℃和43℃下培养,其中在40℃条件下培养6 h,43℃条件下培养1 h。用MTT法检测PDSW联合热疗在不同时间点的细胞活力,同时检测细胞克隆形成率,以及运用流式细胞分析检测细胞凋亡情况。结果表明,在热疗情况下,PDSW能抑制SGC-7901细胞的增殖,影响细胞克隆的形成;同时,PDSW联合热疗(40℃6 h和43℃1 h)后能显著增加SGC-7901细胞的凋亡。以上结果表明,PDSW联合热疗可使人胃癌细胞SGC-7901活力降低,增加胃癌细胞凋亡,具有抗肿瘤作用。
To explore the effects of physiological deep sea water(PDSW) combined with hyperthermia therapy on activity and apoptosis of human gastric cancer cells SGC-7901,we cultured the gastric cancer SGC-7901 cells and randomly divided them into experimental group,control group and blank group. We added PDSW to the experimental group,added normal saline to the control group,and had no treatment on the blank group. We cultured the groups under 37℃,40℃ and 43℃,respectively,and 40℃ for 6 h,43℃ for 1 h. We used MTT method to detect the cell activity at different time points of PDSW combined with hyperthermia therapy. We also detected the cell colony formation rate,and detected the cell apoptosis by flow cytometry method. The results show that PDSW would inhibit the proliferation of SGC-7901 and affect the formation of cell clone combined with hyperthermia therapy. In addition,PDSW combined with hyperthermia therapy(40℃ 6 h and 43℃ 1 h) would significantly increase the apoptosis of SGC-7901 cells. These results showed that PDSW combined with hyperthermia therapy would decrease the activity and increase the apoptosis of human gastric cancer cells SGC-7901,and have anti-tumor effect.
To explore the effects of physiological deep sea water(PDSW) combined with hyperthermia therapy on activity and apoptosis of human gastric cancer cells SGC-7901,we cultured the gastric cancer SGC-7901 cells and randomly divided them into experimental group,control group and blank group. We added PDSW to the experimental group,added normal saline to the control group,and had no treatment on the blank group. We cultured the groups under 37℃,40℃ and 43℃,respectively,and 40℃ for 6 h,43℃ for 1 h. We used MTT method to detect the cell activity at different time points of PDSW combined with hyperthermia therapy. We also detected the cell colony formation rate,and detected the cell apoptosis by flow cytometry method. The results show that PDSW would inhibit the proliferation of SGC-7901 and affect the formation of cell clone combined with hyperthermia therapy. In addition,PDSW combined with hyperthermia therapy(40℃ 6 h and 43℃ 1 h) would significantly increase the apoptosis of SGC-7901 cells. These results showed that PDSW combined with hyperthermia therapy would decrease the activity and increase the apoptosis of human gastric cancer cells SGC-7901,and have anti-tumor effect.
引文
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Dai Y.G.,2014,PDSW combined with whole body hyperthermi treats hepatocellular carcinoma in mice,Dissertation for Ph D.,Kunming Medical University,Supervisor:Cui J.,pp.5(代佑果,2014,“深层海水”与热疗联合治疗肝细胞癌的实验性研究,博士学位论文,昆明医科大学,导师:崔进pp.5)
Hataguchi Y.,Tai H.,Nakajima H.,and Kimata H.,2005,Drink ing deep-sea water restores mineral imbalance in atopi eczema/dermatitis syndrome,European Journal of Clinica Nutrition,59(9):1093-1096
Ji M.M.,Yang J.W.,Du B.,Huang L.M.,Guo J.H.,and Li L.Y.2015,Effect of gastrodin on activity and apoptosis of gastri cancer cells,Xiandai Shengwu Yixue Jinzhan(Progress in Modern Biomedicine),15(13):2421-2424(季蒙蒙,杨金伟,杜斌,黄玲美,郭建辉,李力燕,2015,天麻素对胃癌细胞活力和凋亡的影响,现代生物医学进展,15(13)2421-2424)
Katsuda S.,Yasukawa T.,Nakagawa K.,Miyake M.,Yamasak M.,Katahira K.,Mohri M.,Shimizu T.,and Hazama A.2008,Deep-sea water improves cardiovascular hemodynam ics in Kurosawa and Kusanagi-Hypercholesterolemic(KHCrabbits,Biological and Pharmaceutical Bulletin,31(1):38-44
Kimata H.,Tai H.,Nakagawa K.,Yokoyama Y.,Nakajima H.and Ikegami Y.,2002,Improvement of skin symptoms and mineral imbalance by drinking deep sea water in patient with atopic eczema/dermatitis syndrome(AEDS),Acta Medi ca,45(2):83-84
Li W.M.,Cui J.,Xu P.Y.,and Dai Y.G.,2014,The influence o deep sea water on wound healing of mice,Chongqing Yixu(Chongqing Medical Journal),43(4):462-464(李为明,崔进,徐鹏远,代佑果,2014,深层海水对小鼠创面愈合的促进作用,重庆医学,43(4):462-464)
Ma Y.J.,Li H.,Yan Z.B.,and Gu H.C.,2007,Study on the cel apoptosis induce by intracellular hyperthermia in human lung adenocarcinoma SPC-A1 cells,Shengwu Yixue Gong chengxue Zazhi(Journal of Biomedical Engineering),24(6)1305-1308(马勇杰,李红,鄢祝兵,古宏晨,2007,细胞内磁热疗诱导人肺腺癌细胞SPC-A1凋亡的体外实验研究,生物医学工程学杂志,24(6):1305-1308)
Nakasone T.,and Akeda S.,1999,The application of deep se water in Japan,Proceedings of the Twenty,UJNR Technica Report,28:69-75
Nikfarjam M.,Muralidharan V.,Malcontenti-Wilson C.,and Christophi C.,2005,The apoptotic response of liver and col orectal liver metastases to focal hyperthermic injury,Anti cancer Res.,25(2):1413-1419
Rong Y.,and Mack P.,2000,Apoptosis induced by hyperthermi in Dunn osteosarcoma cell line in vitro,International Journa of Hyperthermia,16(1):19-27
Tang R.,Zhu Z.G.,Qu Y.,Li J.F.,Ji Y.B.,Cai Q.,Liu B.Y.,Yan M.,Yin H.R.,and Lin Y.Z.,2006,The impact of hyperther mic chemotherapy on human gastric cancer cell lines:pre liminary results,Oncology Reports,16(13):631-641
Tang X.Q.,Zuo F.H.,Song Q.X.,and Shenjian G.X.,2007,Char acteristics of deep seawater resource and its application in beverage processing and wine brewing,Yinliao Gongy(Beverage Industry),10(4):4-6(唐心强,左风华,宋巧霞,深见公雄,2007,海洋深层水的资源特性及在饮料制造和酿酒中的应用研究概况,饮料工业,10(4):4-6)
Wu M.Q.,Zhou X.K.,Li P.,Luo Y.,Liu S.S.,and Li D.,2014Neoadjuvant chemotherapy combined with Laparoscopy-as sisted surgery for advanced proximal gastric cancer,Zhong guo Weichuang Waike Zazhi(Chiness Journal of Minimall Invasive Surgery),14(2):109-111(吴梅青,周旭坤,李平罗毅,刘顺顺,李栋,2014,新辅助化疗联合腹腔镜辅助D2近端胃癌根治术治疗近端进展期胃癌,中国微创外科杂志,14(2):109-111)
Zhang J.H.,and Qin L.J.,2012,Research progress of tumor hy perthermia department of physiology,Shiyong Xinnaofe Xueguanbing Zazhi(Practical Journal of Cardiac Cerebra Pneumal and Vascular Disease),20(9):1424-1426(张佳慧秦丽娟,2012,肿瘤热疗的研究进展,实用心脑肺血管病杂志,20(9):1424-1426)
Dai Y.G.,2014,PDSW combined with whole body hyperthermi treats hepatocellular carcinoma in mice,Dissertation for Ph D.,Kunming Medical University,Supervisor:Cui J.,pp.5(代佑果,2014,“深层海水”与热疗联合治疗肝细胞癌的实验性研究,博士学位论文,昆明医科大学,导师:崔进pp.5)
Hataguchi Y.,Tai H.,Nakajima H.,and Kimata H.,2005,Drink ing deep-sea water restores mineral imbalance in atopi eczema/dermatitis syndrome,European Journal of Clinica Nutrition,59(9):1093-1096
Ji M.M.,Yang J.W.,Du B.,Huang L.M.,Guo J.H.,and Li L.Y.2015,Effect of gastrodin on activity and apoptosis of gastri cancer cells,Xiandai Shengwu Yixue Jinzhan(Progress in Modern Biomedicine),15(13):2421-2424(季蒙蒙,杨金伟,杜斌,黄玲美,郭建辉,李力燕,2015,天麻素对胃癌细胞活力和凋亡的影响,现代生物医学进展,15(13)2421-2424)
Katsuda S.,Yasukawa T.,Nakagawa K.,Miyake M.,Yamasak M.,Katahira K.,Mohri M.,Shimizu T.,and Hazama A.2008,Deep-sea water improves cardiovascular hemodynam ics in Kurosawa and Kusanagi-Hypercholesterolemic(KHCrabbits,Biological and Pharmaceutical Bulletin,31(1):38-44
Kimata H.,Tai H.,Nakagawa K.,Yokoyama Y.,Nakajima H.and Ikegami Y.,2002,Improvement of skin symptoms and mineral imbalance by drinking deep sea water in patient with atopic eczema/dermatitis syndrome(AEDS),Acta Medi ca,45(2):83-84
Li W.M.,Cui J.,Xu P.Y.,and Dai Y.G.,2014,The influence o deep sea water on wound healing of mice,Chongqing Yixu(Chongqing Medical Journal),43(4):462-464(李为明,崔进,徐鹏远,代佑果,2014,深层海水对小鼠创面愈合的促进作用,重庆医学,43(4):462-464)
Ma Y.J.,Li H.,Yan Z.B.,and Gu H.C.,2007,Study on the cel apoptosis induce by intracellular hyperthermia in human lung adenocarcinoma SPC-A1 cells,Shengwu Yixue Gong chengxue Zazhi(Journal of Biomedical Engineering),24(6)1305-1308(马勇杰,李红,鄢祝兵,古宏晨,2007,细胞内磁热疗诱导人肺腺癌细胞SPC-A1凋亡的体外实验研究,生物医学工程学杂志,24(6):1305-1308)
Nakasone T.,and Akeda S.,1999,The application of deep se water in Japan,Proceedings of the Twenty,UJNR Technica Report,28:69-75
Nikfarjam M.,Muralidharan V.,Malcontenti-Wilson C.,and Christophi C.,2005,The apoptotic response of liver and col orectal liver metastases to focal hyperthermic injury,Anti cancer Res.,25(2):1413-1419
Rong Y.,and Mack P.,2000,Apoptosis induced by hyperthermi in Dunn osteosarcoma cell line in vitro,International Journa of Hyperthermia,16(1):19-27
Tang R.,Zhu Z.G.,Qu Y.,Li J.F.,Ji Y.B.,Cai Q.,Liu B.Y.,Yan M.,Yin H.R.,and Lin Y.Z.,2006,The impact of hyperther mic chemotherapy on human gastric cancer cell lines:pre liminary results,Oncology Reports,16(13):631-641
Tang X.Q.,Zuo F.H.,Song Q.X.,and Shenjian G.X.,2007,Char acteristics of deep seawater resource and its application in beverage processing and wine brewing,Yinliao Gongy(Beverage Industry),10(4):4-6(唐心强,左风华,宋巧霞,深见公雄,2007,海洋深层水的资源特性及在饮料制造和酿酒中的应用研究概况,饮料工业,10(4):4-6)
Wu M.Q.,Zhou X.K.,Li P.,Luo Y.,Liu S.S.,and Li D.,2014Neoadjuvant chemotherapy combined with Laparoscopy-as sisted surgery for advanced proximal gastric cancer,Zhong guo Weichuang Waike Zazhi(Chiness Journal of Minimall Invasive Surgery),14(2):109-111(吴梅青,周旭坤,李平罗毅,刘顺顺,李栋,2014,新辅助化疗联合腹腔镜辅助D2近端胃癌根治术治疗近端进展期胃癌,中国微创外科杂志,14(2):109-111)
Zhang J.H.,and Qin L.J.,2012,Research progress of tumor hy perthermia department of physiology,Shiyong Xinnaofe Xueguanbing Zazhi(Practical Journal of Cardiac Cerebra Pneumal and Vascular Disease),20(9):1424-1426(张佳慧秦丽娟,2012,肿瘤热疗的研究进展,实用心脑肺血管病杂志,20(9):1424-1426)