生理性深层海水对小鼠高温耐受力的影响
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摘要
目的探讨生理性深层海水(PDSW)对45.0℃高温环境动物耐受性的影响及机制。方法将取自我国海南省海域的深层海水进行制备,制成PDSW,检测所含有的部分元素。20只昆明小鼠,雌雄各半,分为对照组和实验组,对照组小鼠饮用自来水,实验组饮用PDSW,连续15d,然后把各组小鼠放入45.0℃高温环境下饲养,记录每只小鼠死亡时间,直至各组小鼠全部死亡为止。并取每只小鼠脑、肺、心、肝和肾做病理检查。结果无论是雌性小鼠还是雄性小鼠,实验组的小鼠在45.0℃高温下的生存时间比对照组的小鼠长,差异有统计学意义(P<0.05)。并且雌性小鼠的生存时间要比雄性小鼠的生存时间长,差异有统计学意义(P<0.05)。组织学分析表明PDSW对实验小鼠的脑、心、肺、肝、肾等器官均有保护作用。Western blot检测发现,实验组小鼠肝组织HSP72表达较对照组强,差异有统计学意义(P<0.05)。结论 PDSW可以增强小鼠对45.0℃高温环境的耐受性,其机制考虑与PDSW促进热休克蛋白表达有关。
Objective To explore the effects of physiological deep-sea water(PDSW)on hyperthermal tolerance of Kunming(KM)mice in the 45.0 ℃ environment.Methods Deep-sea water from the south Chinese sea was processed,and the metallic elements dissolved in the DSW were analysed.The mice were randomly divided into 2groups:the control group received tap water;the experimental group treated with PDSW for 15 d.And then the mice were fed in the 45.0℃conditions.The survival time and histomorphometric analyses of the brain,lung,heart,liver and kidney were investigated.Results The survival time in PDSW-fed group was significantly longer than that of the control group(P<0.05).Moreover,histomorphometric analyses showed that PDSW could protect the brain,lung,heart,liver and kidney of KM mice from the 45.0℃conditions.The results of western blot revealed that expression of HSP72 of liver tissues for PDSW-fed group substantially increased,when compared with the control mice(P<0.05).Conclusion PDSW could improve hyperthermal tolerance of KM mice,which maybe in the relation with expression of HSP72 promoted by PDSW.
Objective To explore the effects of physiological deep-sea water(PDSW)on hyperthermal tolerance of Kunming(KM)mice in the 45.0 ℃ environment.Methods Deep-sea water from the south Chinese sea was processed,and the metallic elements dissolved in the DSW were analysed.The mice were randomly divided into 2groups:the control group received tap water;the experimental group treated with PDSW for 15 d.And then the mice were fed in the 45.0℃conditions.The survival time and histomorphometric analyses of the brain,lung,heart,liver and kidney were investigated.Results The survival time in PDSW-fed group was significantly longer than that of the control group(P<0.05).Moreover,histomorphometric analyses showed that PDSW could protect the brain,lung,heart,liver and kidney of KM mice from the 45.0℃conditions.The results of western blot revealed that expression of HSP72 of liver tissues for PDSW-fed group substantially increased,when compared with the control mice(P<0.05).Conclusion PDSW could improve hyperthermal tolerance of KM mice,which maybe in the relation with expression of HSP72 promoted by PDSW.
引文
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[16]李为明,崔进,徐鹏远,等.“深层海水”对小鼠耐受力的影响[J].天然产物研究与开发,2013,25(7):826-829.
[17]李为明,崔进,徐鹏远,等.“深层海水”对小鼠免疫功能的影响[J].天然产物研究与开发,2013,25(7):903-906.
[18]李为明,崔进,徐鹏远,等.深层海水对小鼠创面愈合的促进作用[J].重庆医学,2014,43(4):462-464.
[19]Mokuno Y,Berthiaume F,Tanimura Y,et al.Heat shock preconditioning inhibits CD4+T lymphocyte activation in transplanted fatty rat livers[J].J Surg Res,2006,135:92-99.
[2]刘珈.肿瘤热疗技术与临床实践[M].北京:中国医药科技出版社,2009:243-250.
[3]Hwang HS,Kim HA,Lee SH,et al.Anti-obesity and antidiabetic effects of deep sea water on ob/ob mice[J].Mar Biotechnol,2009,11(4):531-539.
[4]Zhao C,Dai C,Chen X.Whole-body hyperthermia combined with hyperthermic intraperitoneal chemotherapy for the treatment of stageⅣadvanced gastric cancer[J].Int J Hyperthermia,2012,28(8):735-741.
[5]Bakshandeh-Bath A,Stoltz AS,Homann N,et al.Preclinical and clinical aspects of carboplatin and gemcitabine combined with whole-bodyhyperthermia for pancreatic adenocarcinoma[J].Anticancer Res,2009,29(8):3069-3077.
[6]Atmaca A,Al-Batran SE,Neumann A,et al.Whole-bodyhyperthermia(WBH)in combination with carboplatin in patients with recurrent ovarian cancer-aphaseⅡstudy[J].Gynecol Oncol,2009,112(2):384-388.
[7]Jia D,Rao W,Wang C,Jin C,et al.Inhibition of B16murine melanoma metastasis and enhancement of immunity by fever-range whole body hyperthermia[J].Int J Hyperthermia,2011,27(3):275-285.
[8]唐劲天,洛小林,朱京丽.全身加温治疗的现状与展望[J].中华放射肿瘤学杂,2000,9(2):140-143.
[9]Tsuchiya Y,Nakamura K,Sekikawa H,et al.Subacute effects of deep-sea water from the Japan Sea on blood examination values in mice[J].Environ Health Prev Med,2002,7(5):189-192.
[10]Kimata H,Tai H,Nakagawa K,et al.Improvement of skin symptoms and mineral imbalance by drinking deep sea water in patients with atopic eczema/derma-titis syndrome(AEDS)[J].Acta Medica(Hradec Kralove),2002,45(2):83-84.
[11]Hataguchi Y,Tai H,Nakajima H,et al.Drinking deep-sea water restores mineral imbalance in atopic eczema/dermatitis syndrome[J].Euro J Clin Nutrit,2005,59(9):1093-1096.
[12]Yoshioka S,Hamada A,Cui T,et al.Pharmacological activity of deep-sea water:examination of hyperlipemia prevention and medical treatment effect[J].Biol Pharm Bull,2004,27(11):1784-1787.
[13]Chi CW.Dietary deep seawater and hepatic steatosis[J].J Chin Med Assoc,2013,76(3):121-122.
[14]Katsuda S,Yasukawa T,Nakagawa K,et al.Deep-sea water improves cardiovascular hemodynamics in Kurosawa and Kusanagi-hypercholesterolemic(KHC)rabbits[J].Biol Pharm Bull,2008,31(1):38-44.
[15]Hou CW,Tsai YS,Jean WH,et al.Deep ocean mineral water accelerates recovery from physical fatigue[J].J Int Soc Sports Nutr,2013,10(1):1-7.
[16]李为明,崔进,徐鹏远,等.“深层海水”对小鼠耐受力的影响[J].天然产物研究与开发,2013,25(7):826-829.
[17]李为明,崔进,徐鹏远,等.“深层海水”对小鼠免疫功能的影响[J].天然产物研究与开发,2013,25(7):903-906.
[18]李为明,崔进,徐鹏远,等.深层海水对小鼠创面愈合的促进作用[J].重庆医学,2014,43(4):462-464.
[19]Mokuno Y,Berthiaume F,Tanimura Y,et al.Heat shock preconditioning inhibits CD4+T lymphocyte activation in transplanted fatty rat livers[J].J Surg Res,2006,135:92-99.