生理性深层海水联合热疗治疗肝细胞癌的体外抑瘤研究
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摘要
目的探讨生理性深层海水(PDSW)联合热疗对肝细胞癌的体外抑瘤作用。方法将取自我国海南省海域的深层海水进行制备,制成PDSW,检测所含有的部分元素。体外培养的正常肝细胞和人肝癌QGY-7703细胞被随机分为PDSW组和生理盐水组,PDSW组加入PDSW,生理盐水组给予等量生理盐水,24h后,每天分别接受40℃热疗6h和43℃热疗1h,在热疗后的24、48、72h,用MTT法检测热疗结合PDSW对正常肝细胞及人肝癌QGY-7703细胞的抑制率。同时检测PDSW及生理盐水在40℃6h连续10d状态下对人肝癌QGY-7703细胞克隆形成率的影响。结果 MTT检测结果显示肿瘤抑制率在两组均呈时间和浓度依赖性。PDSW组的肿瘤抑制率明显较生理盐水组高,差异有统计学意义(P<0.05)。PDSW组对正常肝细胞的生长抑制率明显低于生理盐水组。此外,PDSW组的肿瘤细胞克隆形成率较生理盐水组低,差异有统计学意义(P<0.05)。结论PDSW能够提高正常肝细胞对热的耐受性。当联合热疗时,可明显抑制人肝癌QGY-7703细胞生长。
Objective To explore the anti-cancer effects of physiological deep-sea water(PDSW)combined with hyperthermia for hepatocellular carcinoma in vitro.Methods Deep-sea water(DSW)from the south Chinese sea was processed,and made into PDSW,detection of some elements.In vitro,the cultured normal liver cells and human hepatoma QGY-7703 cells were randomly divided into PDSW group and normal saline(NS)group,the NS group received saline,the PDSW group received different concentrations of PDSW.Two groups were heated respectively to 6hof 40 ℃ or 1hof 43 ℃,24,48,72 hafter the administration of PDSW or saline,the normal liver cells and QGY-7703 cells proliferation capacity and toxicity were investigated by MTT assay.At the same time testing PDSW and NS in 40 ℃ 6hfor 10 dstate of human liver QGY-7703 cell clone formation rate.Results The results of MTT assay showed that tumor inhibitory rate were time and concentration dependent in tow groups.Tumor inhibitory rate of PDSW group in different time was significantly higher than NS group(P<0.05).On the other hand,the inhibitory of hepatocyte for PDSW group in different time were significantly lower than NS group.In addition,the clone formation rate of PDSW group was lower than those of NS group(P<0.05).Conclusion PDSW can improve the heat tolerance of normal liver cells.When combine with heat,it can obviously inhibit the growth of human liver cancer QGY-7703 cells.
Objective To explore the anti-cancer effects of physiological deep-sea water(PDSW)combined with hyperthermia for hepatocellular carcinoma in vitro.Methods Deep-sea water(DSW)from the south Chinese sea was processed,and made into PDSW,detection of some elements.In vitro,the cultured normal liver cells and human hepatoma QGY-7703 cells were randomly divided into PDSW group and normal saline(NS)group,the NS group received saline,the PDSW group received different concentrations of PDSW.Two groups were heated respectively to 6hof 40 ℃ or 1hof 43 ℃,24,48,72 hafter the administration of PDSW or saline,the normal liver cells and QGY-7703 cells proliferation capacity and toxicity were investigated by MTT assay.At the same time testing PDSW and NS in 40 ℃ 6hfor 10 dstate of human liver QGY-7703 cell clone formation rate.Results The results of MTT assay showed that tumor inhibitory rate were time and concentration dependent in tow groups.Tumor inhibitory rate of PDSW group in different time was significantly higher than NS group(P<0.05).On the other hand,the inhibitory of hepatocyte for PDSW group in different time were significantly lower than NS group.In addition,the clone formation rate of PDSW group was lower than those of NS group(P<0.05).Conclusion PDSW can improve the heat tolerance of normal liver cells.When combine with heat,it can obviously inhibit the growth of human liver cancer QGY-7703 cells.
引文
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[12]Chi CW.Dietary deep seawater and hepatic steatosis[J].J Chin Med Assoc,2013,76(3):121-122.
[13]Kim S,Chun SY,Lee DH,et al.Mineral-enriched deepsea water inhibits the metastatic potential of human breast cancer cell lines[J].Int J Oncol,2013,43(5):1691-1700.
[2]席子明,张军,马远方.热疗对SMMC-7721肝癌细胞形态、增殖、凋亡的影响[J].重庆医学,2010,39(3):280-282,385-386.
[3]Gadaleta-Caldarola G,Infusino S,Galise IA,et al.Sorafenib and locoregional deep electro-hyperthermia in advanced hepatocellular carcinoma:A phaseⅡstudy[J].Oncol Lett,2014,8(4):1783-1787.
[4]Hildebrandt B,Wust P,Ahlers O,et al.The cellular and molecular basis of hyperthermia[J].Crit Rev Oncol Hematol,2002,43(1):33-56.
[5]Kalamida D,Karagounis IV,Mitrakas AA,et al.Feverrange hyperthermia vs.hypothermia effect on cancer cell viability,proliferation and HSP90expression[J].PLoS One,2015,10(1):e0116021.
[6]Neill KL,Fairbairn DW,Smith MJ,et al.Critical parameters influencing hyperthermia-induced apoptosis in hum an lymphoid cell lines[J].Apoptosis,1998,104(3):369-375.
[7]崔进.“深层海水”在医学领域中的研究[J].昆明医学院学报,2011,32(8):1-2,8.
[8]Tsuchiya Y,Nakamura K,Sekikawa H,et al.Subacute effects of deep-sea water from the Japan Sea on blood examination values in mice[J].Environ Health Prev Med,2002,7(5):189-192.
[9]Kimata H,Tai H,Nakagawa K,et al.Improvement of skin symptoms and mineral imbalance by drinking deep sea water in patients with atopic eczema/derma-titis syndrome(AEDS)[J].Acta Medica(Hradec Kralove),2002,45(2):83-84.
[10]Hataguchi Y,Tai H,Nakajima H,et al.Drinking deep-sea water restores mineral imbalance in atopic eczema/dermatitis syndrome[J].Eur J Clin Nutr,2005,59(9):1093-1096.
[11]Yoshioka S,Hamada A,Cui T,et al.Pharmacological activity of deep-sea water:examination of hyperlipemia prevention and medical treatment effect[J].Biol Pharm Bull,2004,27(11):1784-1787.
[12]Chi CW.Dietary deep seawater and hepatic steatosis[J].J Chin Med Assoc,2013,76(3):121-122.
[13]Kim S,Chun SY,Lee DH,et al.Mineral-enriched deepsea water inhibits the metastatic potential of human breast cancer cell lines[J].Int J Oncol,2013,43(5):1691-1700.