NaCl和pH对普通变形杆菌和蜂房哈弗尼菌碳源代谢能力的影响
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摘要
本文以轻腌大黄鱼优势菌(普通变形杆菌和蜂房哈弗尼菌)为研究对象,利用BIOLOG分析了两种菌在5℃条件下,不同NaCl浓度和pH水平胁迫对其碳源代谢的影响,通过丰富度指数、孔平均颜色变化率及利用面积,结合主成分分析法评价其碳源代谢能力。结果表明,普通变形杆菌和蜂房哈弗尼菌利用的碳源种类均以糖类为主,其他依次为羧酸类、氨基酸类和己糖磷酸类。NaCl浓度对两株菌的碳源利用能力影响随其浓度升高而增强,NaCl增加到5%时,几乎完全抑制了两株菌对碳源的利用。pH在5.0～7.0范围内,其对两株菌的碳源利用抑制效应随p H降低而增大,但总体抑制效应不显著。结论:NaCl浓度的增加能够逐渐抑制两株菌的碳源代谢,增加到3%时几乎完全被抑制,在pH5.0～7.0的范围内,对两株菌的碳源代谢影响不大。
Proteus vulgaris and Hafnia alvei were spoilage organisms isolated from lightly-salted large yellow croaker at 5 ℃.To analyze the effects of environmental factors on the carbon source metabolism of Proteus vulgaris and Hafnia alvei,BIOLOG was used to analyze the carbon source metabolism of two strains under different NaCl concentration and p H conditions at 5 ℃.The carbon source metabolism ability of Proteus vulgaris and Hafnia alvei was evaluated by the number of positive wells,the average well colour development,the utilization area and principal component analysis.The results showed that,the two strains digested similar carbon sources,such as sugars,carboxylic acids,amino acids and hexose phosphates. The effects of NaCl on the metabolism of the two strains increased with the increase of its concentration,and the metabolism was almost completely inhibited under 5% NaCl concentration.By comparison,the effects of pH on the carbon source metabolism of two strains were not obvious. The inhibition effects of p H( 5.0 ～ 7.0) on the carbon source metabolism of the two strains increased with the decrease of p H values,but the overall inhibition effect was not significant.Conclusion: The increase of NaCl concentration could gradually inhibit the carbon metabolism of the two strains. When NaCl concentration increased to 3%,it almost completely inhibited the strains.In the range of pH5.0～7.0,the carbon source metabolism of the two strains had little effect.
Proteus vulgaris and Hafnia alvei were spoilage organisms isolated from lightly-salted large yellow croaker at 5 ℃.To analyze the effects of environmental factors on the carbon source metabolism of Proteus vulgaris and Hafnia alvei,BIOLOG was used to analyze the carbon source metabolism of two strains under different NaCl concentration and p H conditions at 5 ℃.The carbon source metabolism ability of Proteus vulgaris and Hafnia alvei was evaluated by the number of positive wells,the average well colour development,the utilization area and principal component analysis.The results showed that,the two strains digested similar carbon sources,such as sugars,carboxylic acids,amino acids and hexose phosphates. The effects of NaCl on the metabolism of the two strains increased with the increase of its concentration,and the metabolism was almost completely inhibited under 5% NaCl concentration.By comparison,the effects of pH on the carbon source metabolism of two strains were not obvious. The inhibition effects of p H( 5.0 ～ 7.0) on the carbon source metabolism of the two strains increased with the decrease of p H values,but the overall inhibition effect was not significant.Conclusion: The increase of NaCl concentration could gradually inhibit the carbon metabolism of the two strains. When NaCl concentration increased to 3%,it almost completely inhibited the strains.In the range of pH5.0～7.0,the carbon source metabolism of the two strains had little effect.
引文
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[22]曹海军,李永文,雷雨,等.大口鲇致病菌的分离鉴定、系统发育分析及相关特性的研究[J].微生物学报,2007,47(1):1-6.
[23]甘未祺.蜂房哈夫尼菌群体感应信号分子基因表达的研究[D].大连:大连工业大学,2016.
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[25]孔西曼,张公亮,王佳莹,等.环境因素对即食海参蜂房哈夫尼菌群体感应的影响[J].现代食品科技,2017,33(1):87-92.
[26]吴义龙,刘可姝,费荣梅,等.普通变形杆菌生物被膜形成能力及特性分析[J].畜牧与兽医,2014,46(8):13-17.
[27]马艳,李婷婷,崔方超,等.不同培养条件及群体感应信号分子对蜂房哈夫尼亚菌生物被膜的影响[J].食品科学,2017,38(6):42-47.
[28]沙月霞.红枣贮藏期果面微生物对碳源的利用及主成分分析[J].微生物学通报,2011,38(1):35-44.
[2]Zhu J,Zhao A,Feng L,et al. Quorum sensing signals affect spoilage of refrigerated large yellow croaker(Pseudosciaena crocea)by Shewanella baltica[J]. International Journal of Food Microbiology,2016,217:146-155.
[3]杜东霞.生长谱法测定微生物碳源谱的实验探索[J].菏泽学院学报,2012,34(5):102-104.
[4]郭全友,修艳辉,王鲁民,等.鱼源莓实假单胞菌生长动力学与碳源利用分析[J].农业机械学报,2017,48(6):319-327.
[5]刘玮,林建强,刘相梅,等,核磁共振技术在微生物代谢研究中的应用[J].微生物学通报,2007,34(1):157-160.
[6]吴雪楠,孙菁菁,罗园园,等.草甘膦对土壤微生物能量代谢的影响[J].化学与生物工程,2016,33(4):18-21.
[7]韦洪娟,林贞建,李德海,等.单菌多次级代谢产物方法及其在微生物代谢产物研究中的应用[J].微生物学报,2010,50(6):701-709.
[8]高惠玲,袁其朋,周延,等.两种碳源对纤维单胞菌生长及代谢的影响[J].北京化工大学学报自然科学版,2004,31(6):40-43.
[9]寇艳波.里氏木霉纤维素酶诱导表达过程中碳源感应机制的研究[D].济南:山东大学,2015.
[10]宋收,陈晓明,肖伟,等.基于BIOLOG指纹解析土壤可培微生物对铀污染的响应[J].核农学报,2016,30(6):1169-117.
[11]弓湃,尚立国,战嵛华,等.假单胞菌碳代谢抑制调控系统各组分的功能及作用机制[J].中国农业科技导报,2014,16(2):67-73.
[12]林文娜,李亮,王瑞,等.细菌碳代谢抑制作用的研究进展[J].中国农业科技导报,2011,13(2):46-52.
[13]Mariona Arroyo,David Aldred,Naresh Magan.Environmental factors and preservatives affect carbon utilization patterns and niche overlap of food spoilage fungi[J].Fungal Ecology,2008,1(1):24-32.
[14]李雅乾,周于聪,陈捷.真菌碳代谢抑制机制研究进展[J].中国科技论文在线,2016.
[15] Josef Deutscher.The mechanisms of carbon catabolite repression in bacteria[J].Current Opinion in Microbiology,2008,11(2):87-93.
[16]朱彦祺.生鲜/轻腌大黄鱼货架期及其优势腐败菌特性预测评估系统的构建[D].上海:上海理工大学,2017.
[17]Guo Quanyou,Zhu Yanqi,Wang Lumin,et al. Shelf life prediction and Bacterial flora for the fresh and lightly salted Pseudosciaena crocea stored at different temperatures[J].Emirates Journal of Food and Agriculture,2017,30(1):39-48.
[18]韩承义,王联珠,郭全友,等.SC/T3216-2016盐制大黄鱼[S].
[19]Rogers B F,R L Tate.Temporal analysis of the soil microbial community along atoposequence in Pinel and soils[J]. Soil Biology&Biochemistry,2001,33(10):1389-1401.
[20]Garland J,Mills A L. Classification and Characterization of heterotrophic microbial communities on the basis of patterns of community-levelsole-carbon-source utilization[J].Applied and Environmental Microbiology,1991,57(8):2351-2359.
[21]董秀黄,杨红梅,王芸,等.BIOLOG碳源筛选与分离技术结合研究新疆哈密南湖嗜盐菌多样性[J].新疆农业科学,2013,50(8):52-57.
[22]曹海军,李永文,雷雨,等.大口鲇致病菌的分离鉴定、系统发育分析及相关特性的研究[J].微生物学报,2007,47(1):1-6.
[23]甘未祺.蜂房哈夫尼菌群体感应信号分子基因表达的研究[D].大连:大连工业大学,2016.
[24]Sammar Khalil,Beatrix W Alsanius.Utilisation of carbon sources by Pythium,Phytophthora and Fusarium species as determined by biolog microplate assay[J].The Open Microbiology Journal,2009,3(1):9-14.
[25]孔西曼,张公亮,王佳莹,等.环境因素对即食海参蜂房哈夫尼菌群体感应的影响[J].现代食品科技,2017,33(1):87-92.
[26]吴义龙,刘可姝,费荣梅,等.普通变形杆菌生物被膜形成能力及特性分析[J].畜牧与兽医,2014,46(8):13-17.
[27]马艳,李婷婷,崔方超,等.不同培养条件及群体感应信号分子对蜂房哈夫尼亚菌生物被膜的影响[J].食品科学,2017,38(6):42-47.
[28]沙月霞.红枣贮藏期果面微生物对碳源的利用及主成分分析[J].微生物学通报,2011,38(1):35-44.