清络通痹方与精简方的药效学比较
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摘要
目的:探讨清络通痹方精简前后的药效学作用及影响机制。方法:DBA/1小鼠,复制胶原性关节炎(CIA)模型,随机分为空白组,模型组,清络通痹精简方组(8.4 g·kg-1),清络通痹原方组(13 g·kg-1)及雷公藤多苷片组(9 mg·kg-1),观察一般情况及关节肿胀,ig给药4周后,检测血清中白介素-1β(IL-1β),肿瘤坏死因子-α(TNF-α),抗Ⅱ型胶原抗体(抗CⅡ抗体)含量;踝关节HE染色观察病理学改变及病理评分。结果:与正常组比较,模型组小鼠关节肿胀明显,关节炎评分,TNF-α,IL-1β和抗CⅡ抗体水平明显升高(P<0.01);与模型组比较,清络通痹精简方及原方、雷公藤多苷均能明显抑制小鼠足肿胀,降低关节炎评分,降低血清中TNF-α,IL-1β和抗CⅡ抗体水平(P<0.01,P<0.05);病理学检查提示可减轻关节病理损伤,关节总病理评分降低(P<0.01)。结论:清络通痹精简方将不同性味、不同功效药物合理配伍,治疗效果和作用机制与原方相似。
Objective: To observe pharmacodynamic effects and mechanism of simplified Qingluo Tongbi prescription( SQLT). Method: The model of rheumatoid arthritis was induced by collagen Ⅱ in DBA /1mice. The mice were randomly divided into blank group,collagen-induced arthritis( CIA) group,SQLT group( 8. 4 g·kg-1),original Qingluo Tongbi prescription( QLT) group( 13 g·kg-1) and polyglucosides of Triplegium wilfordii( T Ⅱ) group( 9 mg·kg-1). General conditions and joint swelling were observed. All groups were treated with corresponding drugs through the intragastric administration for four weeks. Interleukin-1β( IL-1β),tumor necrosis factor-α( TNF-α),anti collagen Ⅱ( C Ⅱ) antibody in serum were measured,and hematoxylin-eosin( HE) staining was conducted to observe the histopathologic changes and pathological score of ankle tissues.Result: Compared with the normal group,the model group showed significant joint swelling and increases in TNF-α,IL-1β and anti CⅡ antibody,with high ankle osteoarthritis scores( P < 0. 01). Compared with the CIA group,QLT,SQLT and T Ⅱ groups showed notable reductions in the paws swelling,arthritis scores,contents of IL-1β,TNF-α and anti CⅡ antibody in serum( P < 0. 01,P < 0. 05),and inhibition in the histological damage and cumulative arthritis injury scores. Conclusion: SQLT rationally integrates herbs with different properties,tastes and efficacies,with a similar effect and mechanism with the original prescription.
Objective: To observe pharmacodynamic effects and mechanism of simplified Qingluo Tongbi prescription( SQLT). Method: The model of rheumatoid arthritis was induced by collagen Ⅱ in DBA /1mice. The mice were randomly divided into blank group,collagen-induced arthritis( CIA) group,SQLT group( 8. 4 g·kg-1),original Qingluo Tongbi prescription( QLT) group( 13 g·kg-1) and polyglucosides of Triplegium wilfordii( T Ⅱ) group( 9 mg·kg-1). General conditions and joint swelling were observed. All groups were treated with corresponding drugs through the intragastric administration for four weeks. Interleukin-1β( IL-1β),tumor necrosis factor-α( TNF-α),anti collagen Ⅱ( C Ⅱ) antibody in serum were measured,and hematoxylin-eosin( HE) staining was conducted to observe the histopathologic changes and pathological score of ankle tissues.Result: Compared with the normal group,the model group showed significant joint swelling and increases in TNF-α,IL-1β and anti CⅡ antibody,with high ankle osteoarthritis scores( P < 0. 01). Compared with the CIA group,QLT,SQLT and T Ⅱ groups showed notable reductions in the paws swelling,arthritis scores,contents of IL-1β,TNF-α and anti CⅡ antibody in serum( P < 0. 01,P < 0. 05),and inhibition in the histological damage and cumulative arthritis injury scores. Conclusion: SQLT rationally integrates herbs with different properties,tastes and efficacies,with a similar effect and mechanism with the original prescription.
引文
[1]周学平,周仲瑛,金妙文,等.清络通痹颗粒治疗类风湿关节炎阴虚络热证63例临床观察[J].中医杂志,2003,44(3):191-193.
[2]张玉萌,朱丽萍.雷公藤制剂致肝毒性,生殖毒性和血液系统毒性不良反应回顾性分析[J].中国药物应用与监测,2014,11(3):173-176.
[3]周学平,周玲玲,王旭.基于“异类相制”理论探讨中药复方的配伍减毒作用[J].中医杂志,2013,54(4):271-273
[4]周聪,周玲玲,柳璋璞,等.基于肝毒性的雷公藤复方配伍减毒作用及机制研究[J].中药药理与临床,2013,29(5):106-109.
[5]刘蒙竹,张新龙,潘林梅,等.清络通痹方中雷公藤配伍减毒的化学基础研究[J].中国实验方剂学杂志,2014,20(7):107-122.
[6]Brand D D,Kang A H,Rosloniec E F.The mouse model of collagen-induced arthritis[M].Autoimmunity:Humana Press,2004:295-312.
[7]Lin N,Liu C,Xiao C,et al.Triptolide,a diterpenoid triepoxide,suppresses inflammation and cartilage destruction in collagen-induced arthritis mice[J].Biochem Pharmacol,2007,73(1):136-146.
[8]宋珊珊,张玲玲,魏伟.实验性关节炎动物模型建立及病理机制研究进展[J].中国药理学通报,2012,27(12):1648-1653.
[9]Billiau A,Matthys P.Collagen-induced arthritis and related animal models:how much of their pathogenesis is auto-immune,how much is auto-inflammatory[J].Cytokine Growth Factor Rev,2011,22(5):339-344.
[10]杜中平.祛痹方对Ⅱ型胶原诱导的免疫性关节炎大鼠的治疗作用及其机制探讨[D].北京:中国中医科学院,2012.
[11]Brennan F M,Mc Innes I B.Evidence that cytokines play a role in rheumatoid arthritis[J].J Clin Invest,2008,118(11):3537-3545
[12]王钢,王丽琴,王佳,等.秦艽醇提物对胶原诱导性关节炎大鼠血清抗-CCP及TNF-α的影响[J].中国实验方剂学杂志,2013,19(19):302-305.
[13]Gravallese E M,Goldring S R.Cellular mechanisms and the role of cytokines in bone erosions in rheumatoid arthritis[J].Arthritis Rheum,2000,43(10):2143-2151.
[14]Burmester G R,Feist E,Drner T.Emerging cell and cytokine targets in rheumatoid arthritis[J].Nat Rev Rheumatol,2014,10(2):77-88.
[15]周学平,周玲玲,宋耀鸿,等.清络通痹颗粒对佐剂性关节炎大鼠炎症细胞因子的影响[J].中国免疫学杂志,2003,19(8):545-547.
[16]周玲玲,周学平,梁卫,等.清络通痹颗粒对胶原关节炎大鼠的保护作用及机制研究[J].中药药理与临床,2011,27(2):109-112.
[2]张玉萌,朱丽萍.雷公藤制剂致肝毒性,生殖毒性和血液系统毒性不良反应回顾性分析[J].中国药物应用与监测,2014,11(3):173-176.
[3]周学平,周玲玲,王旭.基于“异类相制”理论探讨中药复方的配伍减毒作用[J].中医杂志,2013,54(4):271-273
[4]周聪,周玲玲,柳璋璞,等.基于肝毒性的雷公藤复方配伍减毒作用及机制研究[J].中药药理与临床,2013,29(5):106-109.
[5]刘蒙竹,张新龙,潘林梅,等.清络通痹方中雷公藤配伍减毒的化学基础研究[J].中国实验方剂学杂志,2014,20(7):107-122.
[6]Brand D D,Kang A H,Rosloniec E F.The mouse model of collagen-induced arthritis[M].Autoimmunity:Humana Press,2004:295-312.
[7]Lin N,Liu C,Xiao C,et al.Triptolide,a diterpenoid triepoxide,suppresses inflammation and cartilage destruction in collagen-induced arthritis mice[J].Biochem Pharmacol,2007,73(1):136-146.
[8]宋珊珊,张玲玲,魏伟.实验性关节炎动物模型建立及病理机制研究进展[J].中国药理学通报,2012,27(12):1648-1653.
[9]Billiau A,Matthys P.Collagen-induced arthritis and related animal models:how much of their pathogenesis is auto-immune,how much is auto-inflammatory[J].Cytokine Growth Factor Rev,2011,22(5):339-344.
[10]杜中平.祛痹方对Ⅱ型胶原诱导的免疫性关节炎大鼠的治疗作用及其机制探讨[D].北京:中国中医科学院,2012.
[11]Brennan F M,Mc Innes I B.Evidence that cytokines play a role in rheumatoid arthritis[J].J Clin Invest,2008,118(11):3537-3545
[12]王钢,王丽琴,王佳,等.秦艽醇提物对胶原诱导性关节炎大鼠血清抗-CCP及TNF-α的影响[J].中国实验方剂学杂志,2013,19(19):302-305.
[13]Gravallese E M,Goldring S R.Cellular mechanisms and the role of cytokines in bone erosions in rheumatoid arthritis[J].Arthritis Rheum,2000,43(10):2143-2151.
[14]Burmester G R,Feist E,Drner T.Emerging cell and cytokine targets in rheumatoid arthritis[J].Nat Rev Rheumatol,2014,10(2):77-88.
[15]周学平,周玲玲,宋耀鸿,等.清络通痹颗粒对佐剂性关节炎大鼠炎症细胞因子的影响[J].中国免疫学杂志,2003,19(8):545-547.
[16]周玲玲,周学平,梁卫,等.清络通痹颗粒对胶原关节炎大鼠的保护作用及机制研究[J].中药药理与临床,2011,27(2):109-112.