帕瑞昔布对大鼠肺缺血再灌注损伤及水通道蛋白1的影响
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摘要
目的探讨帕瑞昔布在大鼠肺缺血再灌注(LIR)损伤的作用及对肺内水通道蛋白-1(AQP-1)的影响。方法24只雄性成年SD大鼠随机分为3组:假手术组(C组)、缺血再灌注组(I组)和帕瑞昔布组(P组)。建立单侧LIR模型,检测肺湿/干重比值(W/D),观察肺组织病理变化,检测肺组织内TNF-α、IL-6含量,AQP-1表达情况。结果 LIR后,肺组织内TNF-α、IL-6明显增多(P<0. 05),肺湿/干重比值也增加(P<0. 05);与I组相比,以上指标在P组中均降低(P<0. 05)。肺组织形态学检测显示,LIR造成肺组织损伤严重,但P组较I组明显减轻。与C组相比,缺血再灌注明显抑制肺组织内AQP-1表达(P<0. 05);但P组表达水平较I组高(P<0. 05)。结论帕瑞昔布可抑制肺缺血再灌注引起的炎症反应,增加AQP-1表达,从而减轻损伤。
Objective To investigate the effects of parecoxib on lung ischemic reperfusion( LIR) injury and aquaporin-1( AQP-1) in rats. Methods Twenty-four male adult SD rats were randomly divided into three groups: sham group( group C),ischemic reperfusion group( group I) and parecoxib group( group P). The unilateral LIR model was established to detect the lung wet/dry weight ratio( W/D),observe the pathological changes of lung tissues,and detect the expression of TNF-,IL-6 and AQP-1 in lung tissues. Results After LIR,TNF-a and IL-6 in lung tissue increased significantly( P<0. 05),and the lung wet/dry weight ratio increased( P<0. 05). Compared with group I,the above indexes were lower in group P( P<0. 05). The morphological examination of lung tissue showed that LIR caused severe lung injury,but the injury in group P was significantly less than that in group I.Compared with group C,ischemic reperfusion significantly inhibited the expression of AQP-1 in lung tissue( P <0. 05),but the expression level in group P was higher than that in group I( P<0. 05). Conclusion Parecoxib can inhibit the inflammatory response induced by lung ischemic reperfusion and increase the expression of AQP-1,thus reducing the injury.
Objective To investigate the effects of parecoxib on lung ischemic reperfusion( LIR) injury and aquaporin-1( AQP-1) in rats. Methods Twenty-four male adult SD rats were randomly divided into three groups: sham group( group C),ischemic reperfusion group( group I) and parecoxib group( group P). The unilateral LIR model was established to detect the lung wet/dry weight ratio( W/D),observe the pathological changes of lung tissues,and detect the expression of TNF-,IL-6 and AQP-1 in lung tissues. Results After LIR,TNF-a and IL-6 in lung tissue increased significantly( P<0. 05),and the lung wet/dry weight ratio increased( P<0. 05). Compared with group I,the above indexes were lower in group P( P<0. 05). The morphological examination of lung tissue showed that LIR caused severe lung injury,but the injury in group P was significantly less than that in group I.Compared with group C,ischemic reperfusion significantly inhibited the expression of AQP-1 in lung tissue( P <0. 05),but the expression level in group P was higher than that in group I( P<0. 05). Conclusion Parecoxib can inhibit the inflammatory response induced by lung ischemic reperfusion and increase the expression of AQP-1,thus reducing the injury.
引文
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[12]陈佳磊,王秋实,邹志田.帕瑞昔布钠对肺移植缺血再灌注损伤的影响[J].哈尔滨医科大学学报,2017,51(1):28-31.
[13]Jin LD,Wang LR,Wu LQ,et al.Effects of COX-2inhibitor on ventilator-induced lung injury in rats[J].International Immunopharmacology,2013,16(2):288.
[14]Zhang Q,Fu J,and Xue X.Inhibition of the expression of aquaporin-1by RNA interference in pulmonary epithelial cells and its effects on water transport[J].Molecular Medicine Reports,2016,13(1):281.
[15]Yamamura Y,Motegi K,Kani K,et al.TNF-alpha inhibits aquaporin5expression in human salivary gland acinar cells via suppression of histone H4acetylation[J].J Cell Mol Med,2012,16(8):1766-1775.
[2]Zhao S,Li XN.Expression of aquaporin-1and aquaporin-3in lung tissue of rat model with ischemia-reperfusion injury[J].Chin Med J(Engl),2010,123(24):3711-3713.
[3]Wang L,Shan Y,Ye Y,et al.COX-2inhibition attenuates lung injury induced by skeletal muscle ischemia reperfusion in rats[J].Int Immunopharmacol,2016,31:116-122.
[4]Zhang T,Ma Y,Xu KQ,et al.Pretreatment of parecoxib attenuates hepatic ischemia/reperfusion injury in rats[J].BMC Anesthesiol,2015,15:165.
[5]Zhao Q,Wu J,Lin Z,et al.Resolvin D1alleviates the lung ischemia reperfusion injury via complement,immunoglobulin,TLR4,and inflammatory factors in rats[J].Inflammation,2016,39(4):1319-1333.
[6]Zhu B,Yang JR,Chen SF,et al.The attenuation of lung ischemia reperfusion injury by oxymatrine[J].Cell Biochem Biophys,2014,70(1):333-336.
[7]Gao W,Zhao J,Kim H,et al.alpha1-Antitrypsin inhibits ischemia reperfusion-induced lung injury by reducing inflammatory response and cell death[J].J Heart Lung Transplant,2014,33(3):309-315.
[8]Krishnadasan B,Naidu BV,Byrne K,et al.The role of proinflammatory cytokines in lung ischemia-reperfusion injury[J].J Thorac Cardiovasc Surg,2003,125(2):261-272.
[9]Schmidt EP,Yang Y,Janssen WJ,et al.The pulmonary endothelial glycocalyx regulates neutrophil adhesion and lung injury during experimental sepsis[J].Nat Med,2012,18(8):1217-1223.
[10]Schett G.Physiological effects of modulating the interleukin-6axis[J].Rheumatology,2018,57:43-50.
[11]张伟洁,郑宏.IL-6介导免疫炎性反应作用及其与疾病关系的研究进展[J].细胞与分子免疫学杂志,2017,33(5):699-703.
[12]陈佳磊,王秋实,邹志田.帕瑞昔布钠对肺移植缺血再灌注损伤的影响[J].哈尔滨医科大学学报,2017,51(1):28-31.
[13]Jin LD,Wang LR,Wu LQ,et al.Effects of COX-2inhibitor on ventilator-induced lung injury in rats[J].International Immunopharmacology,2013,16(2):288.
[14]Zhang Q,Fu J,and Xue X.Inhibition of the expression of aquaporin-1by RNA interference in pulmonary epithelial cells and its effects on water transport[J].Molecular Medicine Reports,2016,13(1):281.
[15]Yamamura Y,Motegi K,Kani K,et al.TNF-alpha inhibits aquaporin5expression in human salivary gland acinar cells via suppression of histone H4acetylation[J].J Cell Mol Med,2012,16(8):1766-1775.