一株拮抗魔芋软腐病的苏云金芽胞杆菌研究
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摘要
魔芋软腐病严重影响着魔芋的生长,本实验室从魔芋软腐病出发,分离筛选出一株对其具有强拮抗作用的菌株16-4,并测定了其抑菌活性.经过形态观察、显微鉴定和分子鉴定确定该菌株是一株苏云金芽孢杆菌.拮抗实验结果表明16-4对魔芋软腐病具有很强的拮抗能力,平板对峙法显示该菌株对魔芋软腐病的抑菌圈达到75.4mm,魔芋块的实验说明16-4菌悬液与病原液3∶1的比例加入能很好地控制魔芋软腐病的发作,模拟田间管理的植株实验,当16-4菌悬液是病原液的3倍时就可达到较理想的防治效果.所有实验结果为魔芋软腐病的生物防治奠定了良好的基础.
The soft rot of konjac has seriously affected the growth of konjac.From the soft rot of konjac,this laboratory has isolated and screened a strain of 16-4 that has strong antagonistic effect on it and tested its antibacterial activity.The strain was identified by morphological observation,microscopic identification and molecular identification to determine the strain is a bacillus thuringiensis.Antagonism to the experimental results show that that 16-4 had a strong antagonistic ability against soft rot of konjac,and the plate confrontation method showed that the antibacterial circle of the strain against soft rot of konjac reached 75.4 mm;the experiment of Konjac block shows that 16-4 bacteria suspension concentrate 3∶1 ratio to join with illness can be very good control of the outbreak of soft rot of konjac.Plant experiments simulating field management showed that an ideal control effect could be achieved when 16-4 bacterial suspension was 3 times of the pathogen solution.All experimental results for the biological control konjac soft rot,laid a good foundation.
The soft rot of konjac has seriously affected the growth of konjac.From the soft rot of konjac,this laboratory has isolated and screened a strain of 16-4 that has strong antagonistic effect on it and tested its antibacterial activity.The strain was identified by morphological observation,microscopic identification and molecular identification to determine the strain is a bacillus thuringiensis.Antagonism to the experimental results show that that 16-4 had a strong antagonistic ability against soft rot of konjac,and the plate confrontation method showed that the antibacterial circle of the strain against soft rot of konjac reached 75.4 mm;the experiment of Konjac block shows that 16-4 bacteria suspension concentrate 3∶1 ratio to join with illness can be very good control of the outbreak of soft rot of konjac.Plant experiments simulating field management showed that an ideal control effect could be achieved when 16-4 bacterial suspension was 3 times of the pathogen solution.All experimental results for the biological control konjac soft rot,laid a good foundation.
引文
[1]魏养利,张雪芳.魔芋的保健功能及发展前景[J].中国林副特产,2017(3):64-66.
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[10]刘士平,蒋维,孔玉珊,等.一种内生枯草孢杆菌及其作用.CN201510475436.O[P].2016-06-15.
[11]朱飞舟,陈利玉,陈汉春.16SrRNA基因序列分析法鉴定病原细菌[J].中南大学学报(医学版),2013,38(10):1035-1041.
[12]Garrity G M,Winters M,Searles D B.Bergry's Manual of Systematic Bacteriology[M].German:Springer-Verlag,2001.
[13]Wu J P,Diao Y,Gu Y C,et al.Infection Pathways of Soft Rot Pathogens on Amorphophallus konjac[J].African Journal of Microbiology Research,2010,4(14):1495-1499.
[14]古洪辉,汪正香,蒋雄,等.魔芋软腐病及其防治研究进展[J].农学学报,2018,8(9):20-24.
[2]丁自立,万中义,矫振彪,等.魔芋软腐病研究进展和对策[J].中国农学通报,2014,30(4):238-241.
[3]吴金平.魔芋软腐病病原菌及其拮抗菌的研究[D].武汉:武汉大学,2010.
[4]黄俊斌,邱仁胜,赵纯森.魔芋软腐病病原菌的鉴定及生物学特性初步研究[J].华中农业大学学报,1999,18(5):413-415.
[5]杨发明,张燕,张发祥,等.魔芋软腐病的发生特点与综合防治技术[J].长江蔬菜,2011(5):36-38.
[6]何斐,崔鸣.魔芋软腐病生物防治研究进展[J].陕西农业科学,2017,63(1):64-67.
[7]张鑫,陈国华,陈芳清,等.魔芋软腐病生物防治研究[J].安徽农业科学,2010,38(17):9058-9059.
[8]Sturz A V,Christie B R,Nowak J.Bacterial Endophytes:Potential Role in Developing Sustainable Systems of Crop Production.[J].Critical Reviews in Plant Sciences,2000,19(1):1-30.
[9]吴金平,刘晓燕,张静柏,等.一株魔芋软腐病拮抗菌的分离鉴定及其活性物质的研究[J].中国农学通报,2011,27(24):302-306.
[10]刘士平,蒋维,孔玉珊,等.一种内生枯草孢杆菌及其作用.CN201510475436.O[P].2016-06-15.
[11]朱飞舟,陈利玉,陈汉春.16SrRNA基因序列分析法鉴定病原细菌[J].中南大学学报(医学版),2013,38(10):1035-1041.
[12]Garrity G M,Winters M,Searles D B.Bergry's Manual of Systematic Bacteriology[M].German:Springer-Verlag,2001.
[13]Wu J P,Diao Y,Gu Y C,et al.Infection Pathways of Soft Rot Pathogens on Amorphophallus konjac[J].African Journal of Microbiology Research,2010,4(14):1495-1499.
[14]古洪辉,汪正香,蒋雄,等.魔芋软腐病及其防治研究进展[J].农学学报,2018,8(9):20-24.