甜椒耐寒种质耐低温IL系的筛选及低温胁迫下生理应答差异
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摘要
本实验以野生甜椒(CA157)、栽培甜椒(CA52)和二者渐渗系(CL122)群体为材料,进行了耐低温IL系的筛选和低温胁迫对甜椒幼苗生理生化指标的测定。结果表明,渐渗系CL122在整个低温处理及恢复过程中与CA157表型相近,耐寒性显著高于CA52。低温胁迫下,对三种材料幼苗的相对电导率、丙二醛(MDA)含量、可溶性糖含量、脯氨酸含量、PSⅡ光化学效率、抗氧化酶活性等指标的分析发现有些指标与抗性密切相关。说明野生甜椒染色体上可能含有控制其耐寒性的关键QTL,幼苗低温应答指标可以鉴定植株的抗性,但应选择差异化较明显的时期。
In this study, the population of wild type sweet pepper(CA157), cultivated sweet pepper(CA52), and introgression lines(ILs) were selected as the material, and the screening of low temperature resistant IL lines and the determination of physiological and biochemical indexes of sweet pepper seedlings under low temperature stress were carried out. The results indicated that the phenotypic performance of CL122 was quite close to CA157 in the whole process of low temperature treatment, and the cold tolerance was significantly stronger than CA52.Under low temperature stress, the analysis of the relative conductivity, MDA content, soluble sugar content,proline content, PSⅡ photochemical efficiency, antioxidant enzyme activity and other indicators of the seedlings of three materials found that some indexs were closely related to resistances. The results suggested that the chromosomes of wild sweet pepper might contain the key QTL to control its cold tolerance. The cold tolerance index of seedlings could be used to identify the resistance of plants, but the period with obvious difference should be selected.
In this study, the population of wild type sweet pepper(CA157), cultivated sweet pepper(CA52), and introgression lines(ILs) were selected as the material, and the screening of low temperature resistant IL lines and the determination of physiological and biochemical indexes of sweet pepper seedlings under low temperature stress were carried out. The results indicated that the phenotypic performance of CL122 was quite close to CA157 in the whole process of low temperature treatment, and the cold tolerance was significantly stronger than CA52.Under low temperature stress, the analysis of the relative conductivity, MDA content, soluble sugar content,proline content, PSⅡ photochemical efficiency, antioxidant enzyme activity and other indicators of the seedlings of three materials found that some indexs were closely related to resistances. The results suggested that the chromosomes of wild sweet pepper might contain the key QTL to control its cold tolerance. The cold tolerance index of seedlings could be used to identify the resistance of plants, but the period with obvious difference should be selected.
引文
Al-aghabary K.,Zhu Z.J.,and Shi Q.H.,2004,Influence of silicon supply on chlorophyll content,chlorophyll fluorescence,and antioxidative enzyme activities in tomato plants under salt stress,J.Plant Nutrition,27(12):2101-2115
Bates L.S.,Waldren R.P.,and Teare I.D.,1973,Rapid determination of free proline for water-stress studies,Plant and Soil,39(1):205-207
Campos P.S.,and Thi A.T.P.,1997,Effects of an abscisic acid pretreatment on membrane leakage and lipid composition of Vigna unguiculata leaf discs subjected to osmotic stress,Plant Sci.,130(1):11-18
Deng R.J.,Fan J.X.,Wang Y.Q.,Jin J.F.,and Liu T.,2014,Physiological responses of pitaya(Hylocereus spp.)seedling to chilling stress and comprehensive evaluation of their cold resistance,Zhiwu Shengli Xuebao(Plant Physiology Journal),50(10):1529-1534(邓仁菊,范建新,王永清,金吉芬,刘涛,2014,火龙果幼苗对低温胁迫的生理响应及其抗寒性综合评价,植物生理学报,50(10):1529-1534)
Flores F.B.,Sánchez-Bel P.,Valdenegro M.,Romojaro F.,Martí-nez-Madrid M.C.,and Egea M.I.,2008,Effect of a pretreatment with nitric oxide on peach(Prunus persica L.)storage at room temperature,Eur.Food Res.Technol.,227(6):1599-1611
Fukao T.,Xu K.,Ronald P.C.,and Bailey-Serres J.,2006,Avariable cluster of ethylene response factor-like genes regulates metabolic and developmental acclimation responses to submergence in rice,Plant Cell,18(8):2021-2034
Gill S.S.,and Tuteja N.,2010,Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants,Plant Physiol.Biochem.,48(12):909-930
Jimenez A.,Hernandez J.A.,Del Rio L.A.,and Sevilla F.,1997,Evidence for the presence of the ascorbate-glutathione cycle in mitochondria and peroxisomes of pea leaves,Plant Physiol.,114(1):275-284
Liu Y.Y.,Li J.Z.,Chen L.,and Cui D.Q.,2006,Effect of low temperature stress on peroxidation product of membrane lipids and activity of related enzymes in wheat seedling leaves,Mailei Zuowu Xuebao(Journal of Triticeae Crops),26(4):70-73(刘艳阳,李俊周,陈磊,崔党群,2006,低温胁迫对小麦叶片细胞膜脂质过氧化产物及相关酶活性的影响,麦类作物学报,26(4):70-73)
Liu Z.J,Cao Z.M.,Zhu J.,and Shen L.B.,2016,Comparative study on the construction of sweet pepper core collection,Dongbei Nongye Daxue Xuebao(Journal of Northeast Agricultural University),47(1):21-29(刘子记,曹振木,朱婕,申龙斌,2016,甜椒核心种质资源比较构建研究,东北农业大学学报,47(1):21-29)
Qin B.X.,Liu L.L.,Han F.Y.,Chen L.,Li N.,Huang M.,and Li R.B.,2015,Study on cold tolerance of chromosome segment substitution lines derived from wild rice(O.rufipogon)as donor at booting stage,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(6):1283-1289(覃宝祥,刘立龙,韩飞怡,陈霖,李楠,黄敏,李容柏,2015,普通野生稻染色体片段代换系的孕穗期耐冷性研究,基因组学与应用生物学,34(6):1283-1289)
Ren Z.H.,Gao J.P.,Li L.G.,Cai X.L.,Huang W.,Chao D.Y.,Zhu M.Z.,Wang Z.Y.,Luan S.,and Lin H.X.,2005,A rice quantitative trait locus for salt tolerance encodes a sodium transporter,Nat.Genet.,37(10):1141-1146
Song L.L.,Gao H.Y.,Chen H.J.,Mao J.L.,Zhou Y.J.,Chen W.X.,and Jiang Y.M.,2009,Effects of short-term anoxic treatment on antioxidant ability and membrane integrity of pos tharvest kiwifruit during storage,Food Chem.,114(4):1216-1221
Takahashi S.,and Murata N.,2008,How do environmental stresses accelerate photoinhibition,Trends Plant Sci.,13(4):178-182
Tanksley S.D.,and McCouch S.R.,1997,Seed banks and molecular maps:unlocking genetic potential from the wild,Science,277(5329):1063-1066
Wingler A.,Mares M.,and Pourtau N.,2004,Spatial patterns and metabolic regulation of photosynthetic parameters during leaf senescence,New Phytologist,161(3):781-789
Xiao J.H.,Gradillo S.,Ahn S.N.,MCcouch S.R.,Tanksley S.D.,Li J.M.,and Yuan L.P.,1996,Genes from wild rice improve yield,Nature,384(6606):223-224
Zhang Z.G.,and Shang Q.M.,2010,Photosynthetic characteristics of pepper leaves under low temperature,weak light and salt stress,Zhongguo Nongye Kexue(Scientia Agriculture Sinica),43(1):123-131(张志刚,尚庆茂,2010,低温,弱光及盐胁迫下辣椒叶片的光合特性,中国农业科学,43(1):123-131)
Bates L.S.,Waldren R.P.,and Teare I.D.,1973,Rapid determination of free proline for water-stress studies,Plant and Soil,39(1):205-207
Campos P.S.,and Thi A.T.P.,1997,Effects of an abscisic acid pretreatment on membrane leakage and lipid composition of Vigna unguiculata leaf discs subjected to osmotic stress,Plant Sci.,130(1):11-18
Deng R.J.,Fan J.X.,Wang Y.Q.,Jin J.F.,and Liu T.,2014,Physiological responses of pitaya(Hylocereus spp.)seedling to chilling stress and comprehensive evaluation of their cold resistance,Zhiwu Shengli Xuebao(Plant Physiology Journal),50(10):1529-1534(邓仁菊,范建新,王永清,金吉芬,刘涛,2014,火龙果幼苗对低温胁迫的生理响应及其抗寒性综合评价,植物生理学报,50(10):1529-1534)
Flores F.B.,Sánchez-Bel P.,Valdenegro M.,Romojaro F.,Martí-nez-Madrid M.C.,and Egea M.I.,2008,Effect of a pretreatment with nitric oxide on peach(Prunus persica L.)storage at room temperature,Eur.Food Res.Technol.,227(6):1599-1611
Fukao T.,Xu K.,Ronald P.C.,and Bailey-Serres J.,2006,Avariable cluster of ethylene response factor-like genes regulates metabolic and developmental acclimation responses to submergence in rice,Plant Cell,18(8):2021-2034
Gill S.S.,and Tuteja N.,2010,Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants,Plant Physiol.Biochem.,48(12):909-930
Jimenez A.,Hernandez J.A.,Del Rio L.A.,and Sevilla F.,1997,Evidence for the presence of the ascorbate-glutathione cycle in mitochondria and peroxisomes of pea leaves,Plant Physiol.,114(1):275-284
Liu Y.Y.,Li J.Z.,Chen L.,and Cui D.Q.,2006,Effect of low temperature stress on peroxidation product of membrane lipids and activity of related enzymes in wheat seedling leaves,Mailei Zuowu Xuebao(Journal of Triticeae Crops),26(4):70-73(刘艳阳,李俊周,陈磊,崔党群,2006,低温胁迫对小麦叶片细胞膜脂质过氧化产物及相关酶活性的影响,麦类作物学报,26(4):70-73)
Liu Z.J,Cao Z.M.,Zhu J.,and Shen L.B.,2016,Comparative study on the construction of sweet pepper core collection,Dongbei Nongye Daxue Xuebao(Journal of Northeast Agricultural University),47(1):21-29(刘子记,曹振木,朱婕,申龙斌,2016,甜椒核心种质资源比较构建研究,东北农业大学学报,47(1):21-29)
Qin B.X.,Liu L.L.,Han F.Y.,Chen L.,Li N.,Huang M.,and Li R.B.,2015,Study on cold tolerance of chromosome segment substitution lines derived from wild rice(O.rufipogon)as donor at booting stage,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(6):1283-1289(覃宝祥,刘立龙,韩飞怡,陈霖,李楠,黄敏,李容柏,2015,普通野生稻染色体片段代换系的孕穗期耐冷性研究,基因组学与应用生物学,34(6):1283-1289)
Ren Z.H.,Gao J.P.,Li L.G.,Cai X.L.,Huang W.,Chao D.Y.,Zhu M.Z.,Wang Z.Y.,Luan S.,and Lin H.X.,2005,A rice quantitative trait locus for salt tolerance encodes a sodium transporter,Nat.Genet.,37(10):1141-1146
Song L.L.,Gao H.Y.,Chen H.J.,Mao J.L.,Zhou Y.J.,Chen W.X.,and Jiang Y.M.,2009,Effects of short-term anoxic treatment on antioxidant ability and membrane integrity of pos tharvest kiwifruit during storage,Food Chem.,114(4):1216-1221
Takahashi S.,and Murata N.,2008,How do environmental stresses accelerate photoinhibition,Trends Plant Sci.,13(4):178-182
Tanksley S.D.,and McCouch S.R.,1997,Seed banks and molecular maps:unlocking genetic potential from the wild,Science,277(5329):1063-1066
Wingler A.,Mares M.,and Pourtau N.,2004,Spatial patterns and metabolic regulation of photosynthetic parameters during leaf senescence,New Phytologist,161(3):781-789
Xiao J.H.,Gradillo S.,Ahn S.N.,MCcouch S.R.,Tanksley S.D.,Li J.M.,and Yuan L.P.,1996,Genes from wild rice improve yield,Nature,384(6606):223-224
Zhang Z.G.,and Shang Q.M.,2010,Photosynthetic characteristics of pepper leaves under low temperature,weak light and salt stress,Zhongguo Nongye Kexue(Scientia Agriculture Sinica),43(1):123-131(张志刚,尚庆茂,2010,低温,弱光及盐胁迫下辣椒叶片的光合特性,中国农业科学,43(1):123-131)