正交试验法优化柴胡利胆颗粒的水提工艺研究
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摘要
为优化柴胡利胆颗粒的水提工艺,以干膏得量和总黄酮含量为指标,重点考察药材浸泡时间(h)、提取溶剂用量(倍)、提取时间(h)和提取次数(次)4个关键因素对该制剂在传统工艺条件下对水提工艺的影响。结果表明,优选的最佳水提取工艺为:加12倍量的水浸泡0.8h、提取2次、每次提取时间为2h;按处方10倍放大实验,在最佳水提工艺条件下,水提干膏平均得量为332.8566g(RSD=2.66%),平均总黄酮含量为1.25mg/g,(RSD=1.21%)。
To optimize the water extraction process of Chaihu Lidan Granules, taking dry extract yield and total flavonoid content as indexes, the effects of four key factors on the water extraction process of the preparation under traditional conditions were investigated,including soaking time(h), solvent dosage(times), extraction time(h) and extraction times(times). The results showed that the optimum water extraction process was as follows: adding 12 times of water, soaking for 0.8 hours, extracting twice and extracting for 2 hours each time; according to the 10 times enlargement experiment, under the optimum water extraction conditions, the average yield of water extract was 332.8566 g(RSD=2.66%), and the average total flavonoid content was 1.25 mg/g(RSD=1.21%).
To optimize the water extraction process of Chaihu Lidan Granules, taking dry extract yield and total flavonoid content as indexes, the effects of four key factors on the water extraction process of the preparation under traditional conditions were investigated,including soaking time(h), solvent dosage(times), extraction time(h) and extraction times(times). The results showed that the optimum water extraction process was as follows: adding 12 times of water, soaking for 0.8 hours, extracting twice and extracting for 2 hours each time; according to the 10 times enlargement experiment, under the optimum water extraction conditions, the average yield of water extract was 332.8566 g(RSD=2.66%), and the average total flavonoid content was 1.25 mg/g(RSD=1.21%).
引文
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[5]刘瑞江,张业旺,闻崇炜,等.正交试验设计和分析方法研究[J].实验技术与管理,2010,27(9):52-55.
[6]贺延新,刘素玲,李郑林,等.正交试验法优选红桃消肿颗粒水提工艺[J].中国药业,2015,24(8):57-58.
[7]高祖新,韩可勤.医药应用概率统计[M].北京:科学技术出版社,2013.
[2]鲍红芳.柴胡利胆汤治疗慢性胆囊炎的临床效果探析[J].中国卫生标准管理,2015,6(9):97-98.
[3]姜义彬.柴胡利胆汤治疗慢性胆囊炎90例临床观察[J].中国社区医师,2014,30(30):111,113.
[4]王玄静.正交试验设计的应用及分析[J].兰州文理学院学报(自然科学版).2016,30(1):17-22.
[5]刘瑞江,张业旺,闻崇炜,等.正交试验设计和分析方法研究[J].实验技术与管理,2010,27(9):52-55.
[6]贺延新,刘素玲,李郑林,等.正交试验法优选红桃消肿颗粒水提工艺[J].中国药业,2015,24(8):57-58.
[7]高祖新,韩可勤.医药应用概率统计[M].北京:科学技术出版社,2013.