百合鳞茎青霉病菌对不同药剂敏感性测定
|
摘要
[目的]研究百合鳞茎青霉病菌对不同药剂的敏感性。[方法]以嘧菌酯、醚菌酯、苯醚甲环唑、丙环唑为试剂,每个试剂均设置0.01、0.10、1.00、5.00、10.00、100.00μg/mL 6个浓度,采用菌丝生长速率法,将供试百合鳞茎青霉病菌单孢菌株在含药平板上分别进行敏感性测定。[结果]醚菌酯和丙环唑对百合鳞茎青霉病菌抑制率较高,苯醚甲环唑和嘧菌酯抑菌率相对较低。百合鳞茎青霉病菌对醚菌酯的EC_(50)小于敏感菌株标准值0.455μg/mL,百合鳞茎青霉病菌对醚菌酯敏感;百合鳞茎青霉病菌对丙环唑和苯醚甲环唑的EC_(50)分别为0.471和0.541μg/mL,均略大于敏感菌株标准值0.455μg/mL,介于0.455～0.911μg/mL,百合鳞茎青霉病菌对丙环唑、苯醚甲环唑敏感性相对较低,即病菌对这2种药剂均表现为低抗;百合鳞茎青霉病菌对嘧菌酯的EC_(50)为8.185μg/mL,大于高抗标准值3.644μg/mL,是敏感菌株标准值0.455μg/mL的17.98倍,即病菌表现为高抗。[结论]该研究为合理选用药剂、防治百合鳞茎青霉病提供参考。
[Objective]To study sensitivity of lily bulb penicillium to different fungicides.[Method]Using pyrimidine,ether ester,phenyl ether methyclozole,procyclazole as reagents,each reagent was set at 0.01,0.10,1.00,5.00,10.00,100.00 μg/mL concentration,using mycelial growth rate method,the sensitivity of the single spore strain of Lilium miltiorrhiza viridis was determined on the tablet containing the drug.[Result]The inhibition rate of ether ester and procyclole was higher and that of benzene ether mezazole and pyrimidine was lower.Through regression analysis,the EC_(50) of Lilium mildew was less than the standard value of 0.455 μg/mL of the sensitive strain,so the Lilium mildew was sensitive to Ethericum;The EC_(50) of Lilium miltiorrhiza was 0.471 and 0.541 μg/mL,respectively,slightly higher than the standard value of 0.455 μg/mL of sensitive strains,between 0.455 and 0.911 μg/mL,Therefore,the sensitivity of Lilium miltiorrhiza to procyclazole and diphenyl ether was relatively low,that was,the bacteria showed low resistance to both of these agents;The EC_(50) for pyrimidine esters was 8.185 μg/mL,which was greater than the high resistance standard value of 3.644 μg/mL.It was 17.98 times the sensitive strain standard value of 0.455 μg/mL,that was,the pathogen was highly resistant.[Conclusion]The study provide a reference for the rational selection of pharmaceutical and prevention and treatment of Lily bulb green mildew.
[Objective]To study sensitivity of lily bulb penicillium to different fungicides.[Method]Using pyrimidine,ether ester,phenyl ether methyclozole,procyclazole as reagents,each reagent was set at 0.01,0.10,1.00,5.00,10.00,100.00 μg/mL concentration,using mycelial growth rate method,the sensitivity of the single spore strain of Lilium miltiorrhiza viridis was determined on the tablet containing the drug.[Result]The inhibition rate of ether ester and procyclole was higher and that of benzene ether mezazole and pyrimidine was lower.Through regression analysis,the EC_(50) of Lilium mildew was less than the standard value of 0.455 μg/mL of the sensitive strain,so the Lilium mildew was sensitive to Ethericum;The EC_(50) of Lilium miltiorrhiza was 0.471 and 0.541 μg/mL,respectively,slightly higher than the standard value of 0.455 μg/mL of sensitive strains,between 0.455 and 0.911 μg/mL,Therefore,the sensitivity of Lilium miltiorrhiza to procyclazole and diphenyl ether was relatively low,that was,the bacteria showed low resistance to both of these agents;The EC_(50) for pyrimidine esters was 8.185 μg/mL,which was greater than the high resistance standard value of 3.644 μg/mL.It was 17.98 times the sensitive strain standard value of 0.455 μg/mL,that was,the pathogen was highly resistant.[Conclusion]The study provide a reference for the rational selection of pharmaceutical and prevention and treatment of Lily bulb green mildew.
引文
[1] 唐祥宁,游春平,刘福秀,等.江西百合病害调查与鉴定[J].江西农业学报,1997,9(4):1-8.
[2] 陈秋萍.福建省百合病害调查初报[J].福建林学院学报,2000,20(2):162-164.
[3] MAGIE R O.Control methods for post-harvest disease of flowere and bulbs[J].ISHS Acta Horticulturae,1970,23(1):4-6.
[4] CHAUHAN S K,SAALTINK G J.A Penicillium attack on hyacinth bulbs as affected by temperature and humidity[J].Netherlands journal of plant pathology,1969,75:197-204.
[5] 王彩霞,毕阳,葛永红.醚菌酯对百合鳞茎青霉病的控制[J].甘肃农业大学学报,2006,41(5):118-121.
[6] 李渐鹏,胡林刚,李永才,等.复合防腐剂对百合青霉病抑制效果的研究[J].食品科学,2014,35(3):71-75.
[7] 杨迎东,冯秀丽,王伟东,等.百合鳞茎青霉病防治药剂筛选[J].北方园艺,2016(5):144-147.
[8] BOLLEN G J.Resistance to benomyl and some related compounds in strains of Penicilium species[J].European journal of plant pathology,1971,77(6):187-193.
[9] 张海良,马辉刚,李湘民,等.辣椒疫霉菌对甲霜灵的敏感性测定[J].江西农业大学学报,2011,33(2):270-274.
[10] 纪明山,祁之秋,王英姿,等.番茄灰霉病菌对嘧霉胺的抗药性[J].植物保护学报,2003,30(4):396-400.
[2] 陈秋萍.福建省百合病害调查初报[J].福建林学院学报,2000,20(2):162-164.
[3] MAGIE R O.Control methods for post-harvest disease of flowere and bulbs[J].ISHS Acta Horticulturae,1970,23(1):4-6.
[4] CHAUHAN S K,SAALTINK G J.A Penicillium attack on hyacinth bulbs as affected by temperature and humidity[J].Netherlands journal of plant pathology,1969,75:197-204.
[5] 王彩霞,毕阳,葛永红.醚菌酯对百合鳞茎青霉病的控制[J].甘肃农业大学学报,2006,41(5):118-121.
[6] 李渐鹏,胡林刚,李永才,等.复合防腐剂对百合青霉病抑制效果的研究[J].食品科学,2014,35(3):71-75.
[7] 杨迎东,冯秀丽,王伟东,等.百合鳞茎青霉病防治药剂筛选[J].北方园艺,2016(5):144-147.
[8] BOLLEN G J.Resistance to benomyl and some related compounds in strains of Penicilium species[J].European journal of plant pathology,1971,77(6):187-193.
[9] 张海良,马辉刚,李湘民,等.辣椒疫霉菌对甲霜灵的敏感性测定[J].江西农业大学学报,2011,33(2):270-274.
[10] 纪明山,祁之秋,王英姿,等.番茄灰霉病菌对嘧霉胺的抗药性[J].植物保护学报,2003,30(4):396-400.