苏木对糖尿病大血管病变中SOD、TNF-α、MCP-1的影响
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摘要
目的:探讨苏木对糖尿病大血管病变大鼠血清超氧化物歧化酶(SOD)、肿瘤坏死因子α(TNF-α)、单核细胞趋化因子1(MCP-1)水平的影响。方法:将24只雄性SD大鼠随机分为4组,每组6只,分别为对照组、模型组、α-硫辛酸组和苏木组。除去对照组,余下的18只大鼠单次腹腔注射STZ50 mg/kg制备糖尿病大鼠模型,对照组予以注射等体积的柠檬酸-柠檬酸钠缓冲液。成功造模后,苏木组给予0.15 g/kg/d的苏木,α-硫辛酸组给予20 mg/kg/d的α-硫辛酸,每日1次给药,连续给药8周。8周末,检测和比较两组大鼠血清中SOD、TNF-α、MCP-1水平的变化。结果:治疗后,苏木组及α-硫辛酸组血清SOD含量均高于模型组(P<0.05),但苏木组和α-硫辛酸组组间比较无统计学差异(P>0.05);苏木组及α-硫辛酸组血清TNF-α、MCP-1含量均显著低于模型组(P<0.05),与对照组没有明显差别(P>0.05)。结论:苏木可提高糖尿病大血管病大鼠血清SOD含量,降低血清TNF-α、MCP-1含量。
Objective: Investigate the effect of Caesalpinia sappan on the levels of serum superoxide dismutase(SOD), tumor necrosis factor(TNF-α) and monocyte chemokine(MCP-1) in diabetic rats with macrovascular disease. Method: 24 male rats were randomly divided into 4 groups, 6 rats in each group, the control group, the model group, the α-lipoic acid group and the Caesalpinia sappan group, respectively. Except the control group, STZ50 mg/kg was injected intraperitoneally in other three groups. The control group was injected with equal volume of citric acid sodium citrate buffer. After successful molding, the Caesalpinia sappan group was given0.15 g/kg/d of Caesalpinia sappan, α-lipoic acid group was given alipoic acid of 20 mg/kg/d, the drug was given 1 times a day for 8 weeks. After 8 weeks, the changes of SOD, TNF-α, MCP-1 in serum of two groups of rats were detected and compared. Results: After treatment, the contents of serum SOD in Caesalpinia sappan group andα-lipoic acid group were higher than those in model group.(P<0.05), there was no statistical difference between two groups yet(P>0.05). The level of serum TNF-α and MCP-1 were significantly lower in the Caesalpinia sappan group and α-lipoic acid group than those in the model group(P<0.05), but there was no obvious differ-ence comparing with the control group(P>0.05). Conclusion: Caesalpinia sappan can increase the content of SOD and decrease the con-tent of TNF-α and MCP-1 in the serum of diabetic rats with macrovascular disease.
Objective: Investigate the effect of Caesalpinia sappan on the levels of serum superoxide dismutase(SOD), tumor necrosis factor(TNF-α) and monocyte chemokine(MCP-1) in diabetic rats with macrovascular disease. Method: 24 male rats were randomly divided into 4 groups, 6 rats in each group, the control group, the model group, the α-lipoic acid group and the Caesalpinia sappan group, respectively. Except the control group, STZ50 mg/kg was injected intraperitoneally in other three groups. The control group was injected with equal volume of citric acid sodium citrate buffer. After successful molding, the Caesalpinia sappan group was given0.15 g/kg/d of Caesalpinia sappan, α-lipoic acid group was given alipoic acid of 20 mg/kg/d, the drug was given 1 times a day for 8 weeks. After 8 weeks, the changes of SOD, TNF-α, MCP-1 in serum of two groups of rats were detected and compared. Results: After treatment, the contents of serum SOD in Caesalpinia sappan group andα-lipoic acid group were higher than those in model group.(P<0.05), there was no statistical difference between two groups yet(P>0.05). The level of serum TNF-α and MCP-1 were significantly lower in the Caesalpinia sappan group and α-lipoic acid group than those in the model group(P<0.05), but there was no obvious differ-ence comparing with the control group(P>0.05). Conclusion: Caesalpinia sappan can increase the content of SOD and decrease the con-tent of TNF-α and MCP-1 in the serum of diabetic rats with macrovascular disease.
引文
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[18]李钰.羊乳中超氧化物歧化酶活性的影响因素研究及应用[D].齐鲁工业大学,2016Li Yu.Study on the influencing factors of Goatmilk superoxide dismutase activity and its application[D].Qilu Industrial University,2016
[19]Jung H,Kim Y Y,Kim B,et al.Improving glycemic control in model mice with type 2 diabetes by increasing superoxide dismutase(SOD)activity using silk fibroin hydrolysate(SFH)[J].Biochemical&Biophysical Research Communications,2017,493(1)
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[21]Koh K K,Han S H,Oh P C,et al.Combination therapy for treatment or prevention of atherosclerosis:focus on the lipid-RAAS interaction[J].Atherosclerosis,2010,209(2):307
[22]车海蓝.葡萄籽提取物原花青素对动脉粥样硬化大鼠ABCA1、TNF--α、MCP--1表达的影响[D].新乡医学院,2014Che Hai-lan.Effect of Grape Seed Extract Proanthocyanidins on the expression of ABCA1,TNF--alpha and MCP--1 in atherosclerotic rats[D].Xinxiang Medical University,2014
[23]Lee J,Lee S,Zhang H,et al.Interaction of IL-6 and TNF-α±contributes to endothelial dysfunction in type 2 diabetic mouse hearts[J].Plos One,2017,12(11):e0187189
[24]李辉,张迪,赵红霞,等.血清IGF-1、TNF-α与糖尿病患者动脉粥样硬化的相关性研究[J].辽宁医学院学报,2017,38(2):22-24Li Hui,Zhang Di,Zhao Hong-xia,et al.Study on the correlation between serum IGF-1,TNF-αand atherosclerosis in diabetic patients[J].Journal of Liaoning Medical University,2017,38(2):22-24
[25]Koulmanda M,Bhasin M,Awdeh Z,et al.The Role of TNF-αin Mice with Type 1-and 2-Diabetes[J].Plos One,2012,7(5):e33254
[26]林杨.MCP-1与2型糖尿病大血管病变关系的临床研究[D].安徽医科大学,2008Lin Yang.The clinical study of the relationship between MCP-1 and type 2 diabetic macroangiopathy[D].Medical University Of Anhui,2008
[27]Wu S Q,Otero M,Unger F M,et al.Anti-inflammatory activity of an ethanolic Caesalpinia sappan extract in human chondrocytes and macrophages[J].Journal of Ethnopharmacology,2011,138(2):364
[28]Uddin G M,Kim C Y,Chung D,et al.One-step isolation of sappanol and brazilin from Caesalpinia sappan and their effects on oxidative stress-induced retinal death[J].BMB Rep,2015,48(5):289-294
[29]张红,章卓,黄小波,等.苏木水煎液对糖尿病肾病大鼠血糖、肾功能和肾脏病理影响[J].时珍国医国药,2011,22(5):1255-1256Zhang Hong,Zhang Zhuo,Huang Xiao-bo,et al.Effect of Caesalpinia Sappan decoction on blood sugar,renal function and kidney pathology of diabetic nephropathy rats[J].Lishizhen Medicine and Materia Medica Research,2011,22(5):1255-1256
[30]李红微.苏木防治早期糖尿病肾病的实验研究[D].哈尔滨医科大学,2010Li Hong-wei.Experimental study on the prevention and treatment of early diabetic nephropathy by Caesalpinia Sappan[D].Harbin Medical Sciences University,2010
[31]刘婷,佟晓哲.巴西苏木素调控自噬对高糖诱导血管内皮细胞损伤的影响[J].中国动脉硬化杂志,2017,25(4):343-346Liu Ting,Tong Xiao-zhe.Effects of Brazilian hematoxylin regulated autophagy on vascular endothelial cell injury induced by high glucose[J].Chinese Journal of Arteriosclerosis,2017,25(4):343-346
[2]Sun W L,Long J,Kang L,et al.Ginseng extract reduces tacrolimus-induced oxidative stress by modulating autophagy in pancreatic beta cells[J].Laboratory investigation;a journal of technical methods and pathology,2017,97(11):1271
[3]Bo J,Xie S,Yi G,et al.Methylglyoxal Impairs Insulin Secretion of Pancreaticβ-Cells through Increased Production of ROS and Mitochondrial Dysfunction Mediated by Upregulation of UCP2 and MAPKs[J].Journal of Diabetes Research,2016,(9):2029854
[4]Obirikorang Y,Obirikorang C,Anto E O,et al.Knowledge of complications of diabetes mellitus among patients visiting the diabetes clinic at Sampa Government Hospital,Ghana:a descriptive study[J].Bmc Public Health,2016,16(1):637
[5]Katakami N.Mechanism of Development of Atherosclerosis and Cardiovascular Disease in Diabetes Mellitus[J].Journal of Atherosclerosis&Thrombosis,2017:Epub ahead of print
[6]Hameed I,Masoodi S R,Mir S A,et al.Type 2 diabetes mellitus:From a metabolic disorder to an inflammatory condition[J].World Journal of Diabetes,2015,6(4):598-612
[7]周贤珍,周毅生.苏木的研究进展[J].广东药学院学报,2017,33(1):136-139Zhou Xian-zhen,Zhou Yi-Sheng.Research progress of Caesalpinia sappan[J].Journal of Guangdong Pharmaceutical University,2017,33(1):136-139
[8]Camara? C,Pucelle M,N?Gre-Salvayre A,et al.Angiogenesis in the atherosclerotic plaque[J].Redox Biology,2017,12:18-34
[9]Schalkwijk C G,Miyata T.Early-and advanced non-enzymatic glycation in diabetic vascular complications:the search for therapeutics[J].Amino Acids,2012,42(4):1193
[10]Tangvarasittichai S.Oxidative stress,insulin resistance,dyslipidemia and type 2 diabetes mellitus[J].World Journal of Diabetes,2015,6(3):456-480
[11]Brownlee M.Biochemistry and molecular cell biology of diabetic complications[J].Nature,2001,414(6865):813-820
[12]Kayama Y,Raaz U,Jagger A,et al.Diabetic Cardiovascular Disease Induced by Oxidative Stress[J].International Journal of Molecular Sciences,2015,16(10):25234-25263
[13]Jha J C,Banal C,Chow B S,et al.Diabetes and kidney disease:Role of oxidative stress[J].Antioxidants&Redox Signaling,2016,25(12):657
[14]Butaeva SG,Ametov AS,Bugrov AV,et al.Glycemic variability and oxidative stress in patients with type 2 diabetes mellitus during combined glucose-lowering therapy[J].Ter Arkh,2017,89(10):36-39
[15]ROS Schaar C E,Dues D J,Spielbauer K K,et al.Mitochondrial and Cytoplasmic ROS Have Opposing Effects on Lifespan[J].Plos Genetics,2015,11(2):e1004972
[16]Nishikawa T,Araki E.Impact of mitochondrial ROS production in the pathogenesis of diabetes mellitus and its complications[J].Antioxidants&Redox Signaling,2007,9(3):343-353
[17]Yang X,Li Y,Li Y,et al.Oxidative Stress-Mediated Atherosclerosis:Mechanisms and Therapies[J].Frontiers in Physiology,2017,8:600
[18]李钰.羊乳中超氧化物歧化酶活性的影响因素研究及应用[D].齐鲁工业大学,2016Li Yu.Study on the influencing factors of Goatmilk superoxide dismutase activity and its application[D].Qilu Industrial University,2016
[19]Jung H,Kim Y Y,Kim B,et al.Improving glycemic control in model mice with type 2 diabetes by increasing superoxide dismutase(SOD)activity using silk fibroin hydrolysate(SFH)[J].Biochemical&Biophysical Research Communications,2017,493(1)
[20]陈燕.丹瓜方对转基因Apo E-/-糖尿病小鼠氧化应激的干预研究[D].福建中医药大学,2014Chen Yan.Study on the intervention of Dan Gua Fang on oxidative stress in transgenic Apo E-/-diabetic mice[D].Fujian University of Traditional Chinese Medicine,2014
[21]Koh K K,Han S H,Oh P C,et al.Combination therapy for treatment or prevention of atherosclerosis:focus on the lipid-RAAS interaction[J].Atherosclerosis,2010,209(2):307
[22]车海蓝.葡萄籽提取物原花青素对动脉粥样硬化大鼠ABCA1、TNF--α、MCP--1表达的影响[D].新乡医学院,2014Che Hai-lan.Effect of Grape Seed Extract Proanthocyanidins on the expression of ABCA1,TNF--alpha and MCP--1 in atherosclerotic rats[D].Xinxiang Medical University,2014
[23]Lee J,Lee S,Zhang H,et al.Interaction of IL-6 and TNF-α±contributes to endothelial dysfunction in type 2 diabetic mouse hearts[J].Plos One,2017,12(11):e0187189
[24]李辉,张迪,赵红霞,等.血清IGF-1、TNF-α与糖尿病患者动脉粥样硬化的相关性研究[J].辽宁医学院学报,2017,38(2):22-24Li Hui,Zhang Di,Zhao Hong-xia,et al.Study on the correlation between serum IGF-1,TNF-αand atherosclerosis in diabetic patients[J].Journal of Liaoning Medical University,2017,38(2):22-24
[25]Koulmanda M,Bhasin M,Awdeh Z,et al.The Role of TNF-αin Mice with Type 1-and 2-Diabetes[J].Plos One,2012,7(5):e33254
[26]林杨.MCP-1与2型糖尿病大血管病变关系的临床研究[D].安徽医科大学,2008Lin Yang.The clinical study of the relationship between MCP-1 and type 2 diabetic macroangiopathy[D].Medical University Of Anhui,2008
[27]Wu S Q,Otero M,Unger F M,et al.Anti-inflammatory activity of an ethanolic Caesalpinia sappan extract in human chondrocytes and macrophages[J].Journal of Ethnopharmacology,2011,138(2):364
[28]Uddin G M,Kim C Y,Chung D,et al.One-step isolation of sappanol and brazilin from Caesalpinia sappan and their effects on oxidative stress-induced retinal death[J].BMB Rep,2015,48(5):289-294
[29]张红,章卓,黄小波,等.苏木水煎液对糖尿病肾病大鼠血糖、肾功能和肾脏病理影响[J].时珍国医国药,2011,22(5):1255-1256Zhang Hong,Zhang Zhuo,Huang Xiao-bo,et al.Effect of Caesalpinia Sappan decoction on blood sugar,renal function and kidney pathology of diabetic nephropathy rats[J].Lishizhen Medicine and Materia Medica Research,2011,22(5):1255-1256
[30]李红微.苏木防治早期糖尿病肾病的实验研究[D].哈尔滨医科大学,2010Li Hong-wei.Experimental study on the prevention and treatment of early diabetic nephropathy by Caesalpinia Sappan[D].Harbin Medical Sciences University,2010
[31]刘婷,佟晓哲.巴西苏木素调控自噬对高糖诱导血管内皮细胞损伤的影响[J].中国动脉硬化杂志,2017,25(4):343-346Liu Ting,Tong Xiao-zhe.Effects of Brazilian hematoxylin regulated autophagy on vascular endothelial cell injury induced by high glucose[J].Chinese Journal of Arteriosclerosis,2017,25(4):343-346