婴幼儿腹泻患者肠道菌群分布特点研究
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摘要
目的研究不同年龄段腹泻患儿肠道菌群分布特点,探讨不同年龄腹泻患儿肠道菌群与疾病的关联。方法选取9例符合临床诊断标准的0~1岁婴儿腹泻患者和8例符合临床诊断标准的1~3岁幼儿腹泻患者的粪便样本,同时于健康儿童中随机选取6例粪便样本作为对照,提取各组对象粪便总DNA,采用PCR-DGGE进行菌群多样性与差异性分析。结果 0~1岁腹泻患儿肠道菌群与健康对照组相比,肠道菌群构成差异显著,条件致病菌巴黎链球菌数量显著增加。1~3岁腹泻患儿肠道菌群与健康对照组相比,条件致病菌解没食子酸链球菌、屎肠球菌数量显著增加,长双歧杆菌数量下降。结论婴幼儿腹泻患者肠道菌群的构成与健康对照组相比差异显著,该特点可作为婴幼儿腹泻早期诊断的实验依据。
Objective To observe the change of intestinal microbiota in various age groups of children with diarrhea,and explore the relationship between intestinal flora and the development of infantile diarrhea.Methods Fecal samples of infants with infantile diarrhea,including 9 infants at age of 0 to 1 year and 8 children at age of 1 to 3 years,were collected,with those of 6 healthy volunteers as the controls.The total DNA of fecal bacteria was extracted;polymerase chain reaction-denaturation gradient gel electrophoresis gene fingerprinting technology was used to detect the structure of intestinal flora in each age group.Results The composition of intestinal microbiota in 0-1 year group was significantly different from that in the control group;the amounts of conditional pathogenic bacteria such as Streptococcus lutetiensis significantly increased.In the 1-3 years group,an overgrowth of Streptococcus gallolyticus and Enterococcus faecium was observed,while the amount of probiotics such as Bifidobacterium longum significantly decreased compared with healthy subjects.Conclusion The diversity and composition of intestinal flora in infants with diarrhea change dramatically,which may serve as an evidence for early diagnosis of infantile diarrhea.
Objective To observe the change of intestinal microbiota in various age groups of children with diarrhea,and explore the relationship between intestinal flora and the development of infantile diarrhea.Methods Fecal samples of infants with infantile diarrhea,including 9 infants at age of 0 to 1 year and 8 children at age of 1 to 3 years,were collected,with those of 6 healthy volunteers as the controls.The total DNA of fecal bacteria was extracted;polymerase chain reaction-denaturation gradient gel electrophoresis gene fingerprinting technology was used to detect the structure of intestinal flora in each age group.Results The composition of intestinal microbiota in 0-1 year group was significantly different from that in the control group;the amounts of conditional pathogenic bacteria such as Streptococcus lutetiensis significantly increased.In the 1-3 years group,an overgrowth of Streptococcus gallolyticus and Enterococcus faecium was observed,while the amount of probiotics such as Bifidobacterium longum significantly decreased compared with healthy subjects.Conclusion The diversity and composition of intestinal flora in infants with diarrhea change dramatically,which may serve as an evidence for early diagnosis of infantile diarrhea.
引文
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[15]Frese SA,Hutton AA,Contreras LN,et al.Persistence of supplemented Bifidobacterium longum subsp.infantis EVC001in breastfed infants[J].mSphere,2017,doi:10.1128/mSphere.00501-17.
[16]Inturri R,Mangano K,Santagati M,et al.Immunomodulatory effects of Bifidobacterium longum W11produced exopolysaccharide on cytokine production[J].Curr Pharm Biotechnol,2017,18(11):883-889.
[17]Cao YN,Feng LJ,Wang BM,et al.Lactobacillus acidophilus and Bifidobacterium longumsupernatants upregulate the serotonin transporter expression in intestinal epithelial cells[J].Saudi J Gastroenterol,2018,24(1):59-66.
[18]FANG Daiqiong,GU Silan,SHI Ding,et al.Relationships between human gut microbiota and occurrence and development of diseases and the mechanisms:Research progress[J].Chin J Microecol,2016,28(5):614-617.(in Chinese)方戴琼,顾思岚,石鼎,等.人体肠道微生态与疾病发生发展的关系及机制研究进展[J].中国微生态学杂志,2016,28(5):614-617.
[19]XIANG Chunshang,LI Lanjuan.Advances in microecology of intestinal bacteria[J].China Basic Sci,2017,19(2):1-9.(in Chinese)项春生,李兰娟.肠道细菌微生态研究进展[J].中国基础科学,2017,19(2):1-9.
[20]GUO Huiling,SHAO Yuyu,MENG Hebilige,et al.Research on the relation between gastrointestinal microbiota and disease[J].J Microbiol,2015,42(2):400-410.(in Chinese)郭慧玲,邵玉宇,孟和毕力格,等.肠道菌群与疾病关系的研究进展[J].微生物学通报,2015,42(2):400-410.
[21]MA Ke,KOU Yan.Analysis of efficacy of microbial ecological agents in treating infantile diarrhea and changes of intestinal flora in children[J].J Colorect&Anal Surg,2016,22(2):122-125.(in Chinese)马科,寇艳.微生态制剂治疗小儿腹泻的疗效及患儿肠道菌群变化分析[J].结直肠肛门外科,2016,22(2):122-125.
[2]Chou VB,Friberg IK,Christian M,et al.Expanding the population coverage of evidence-based interventions with community health workers to save the lives of mothers and children:an analysis of potential global impact using the Lives Saved Tool(LiST)[J].J Glob Health,2017,7(2):20401.
[3]Pavlinac PB,Brander RL,Atlas HE,et al.Interventions to reduce post-acute consequences of diarrheal disease in children:A systematic review[J].BMC Public Health,2018,18(1):208.
[4]ZHENG Yuqiang,ZHANG Penghui,LIU Lan,et al.Pathogenic investigation in diarrhea disease of inpatient infants and children[J].Chongqing Med J,2010,39(7):844-846.(in Chinese)郑玉强,张鹏辉,刘岚,等.婴幼儿腹泻的病原学分析[J].重庆医学,2010,39(7):844-846.
[5]ZHANG Lingling.Analysis of pathogenic bacteria in infants with diarrhea[J].J Biolog Chem,2017,3(1):38-40.(in Chinese)张玲玲.婴幼儿腹泻病原微生物检验结果分析[J].生物化工,2017,3(1):38-40.
[6]Harrington A,Tal-Gan Y.Identification of Streptococcus gallolyticus subsp.gallolyticus(biotype I)competence stimulating peptide pheromone[J].J Bacteriol,2018,doi:10.1128/JB.00709-17.
[7]Aymeric L,Donnadieu F,Mulet C,et al.Colorectal cancer specific conditions promote Streptococcus gallolyticus gut colonization[J].Proc Natl Acad Sci USA,2018,115(2):E283-E291.
[8]朱聪智,黄洁,贾小强.解没食子酸链球菌感染研究进展[J].中国临床研究,2016,29(5):713-715.
[9]朱旗,项勋,段纲,等.牛链球菌研究进展[J].上海畜牧兽医通讯,2017(2):18-21.
[10]Elzein FE,Akhtar MY,Khairallah H,et al.Streptococcus bovis prosthetic valve endocarditis associated with silent colonic carcinoma[J].BMJ Case Rep,2017,doi:10.1136/bcr-2017-219488.
[11]Pushpakumara J,Mudiyanse G,Jayawardana A,et al.Tricuspid Valve Endocarditis due to Streptococcus bovis in a patient with ventricular septal defect:A rare manifestation-case report and review of the literature[J].Case Rep Infect Dis,2017,doi:10.1155/2017/7152902.
[12]Idrees S,Gupta S,Mantilla M,et al.Unusual cause of severe diabetic ketoacidosis precipitated by Streptococcus bovis/equinus(SBSEC)bacteremia:Case report and review of literature[J].ID Cases,2018,11:53-55.
[13]WANG TAO,ZHANG Shiyang,HONG Guolin,et al.Investigation and analysis of Enterococcus faecalis and Enterococcus faecius causing nosocomial infections from 2013to 2015[J].Chin J Nosocomiol,2017,27(1):51-53.(in Chinese)王涛,张世阳,洪国粦,等.2013—2015年医院感染粪肠球菌和屎肠球菌的调查分析[J].中华医院感染学杂志,2017,27(1):51-53.
[14]Martinez JL,Baquero F.Interactions among strategies associated with bacterial infection:Pathogenicity,epidemicity,and antibiotic resistance[J].Clin Microbiol Rev,2002,15(4):647-679.
[15]Frese SA,Hutton AA,Contreras LN,et al.Persistence of supplemented Bifidobacterium longum subsp.infantis EVC001in breastfed infants[J].mSphere,2017,doi:10.1128/mSphere.00501-17.
[16]Inturri R,Mangano K,Santagati M,et al.Immunomodulatory effects of Bifidobacterium longum W11produced exopolysaccharide on cytokine production[J].Curr Pharm Biotechnol,2017,18(11):883-889.
[17]Cao YN,Feng LJ,Wang BM,et al.Lactobacillus acidophilus and Bifidobacterium longumsupernatants upregulate the serotonin transporter expression in intestinal epithelial cells[J].Saudi J Gastroenterol,2018,24(1):59-66.
[18]FANG Daiqiong,GU Silan,SHI Ding,et al.Relationships between human gut microbiota and occurrence and development of diseases and the mechanisms:Research progress[J].Chin J Microecol,2016,28(5):614-617.(in Chinese)方戴琼,顾思岚,石鼎,等.人体肠道微生态与疾病发生发展的关系及机制研究进展[J].中国微生态学杂志,2016,28(5):614-617.
[19]XIANG Chunshang,LI Lanjuan.Advances in microecology of intestinal bacteria[J].China Basic Sci,2017,19(2):1-9.(in Chinese)项春生,李兰娟.肠道细菌微生态研究进展[J].中国基础科学,2017,19(2):1-9.
[20]GUO Huiling,SHAO Yuyu,MENG Hebilige,et al.Research on the relation between gastrointestinal microbiota and disease[J].J Microbiol,2015,42(2):400-410.(in Chinese)郭慧玲,邵玉宇,孟和毕力格,等.肠道菌群与疾病关系的研究进展[J].微生物学通报,2015,42(2):400-410.
[21]MA Ke,KOU Yan.Analysis of efficacy of microbial ecological agents in treating infantile diarrhea and changes of intestinal flora in children[J].J Colorect&Anal Surg,2016,22(2):122-125.(in Chinese)马科,寇艳.微生态制剂治疗小儿腹泻的疗效及患儿肠道菌群变化分析[J].结直肠肛门外科,2016,22(2):122-125.