结肠癌P53、nm23免疫组化表达特征及与临床病理的关联分析
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摘要
目的探讨结肠癌患者P53、nm23的表达及其与相关临床病理特征的关联。方法选取2013年至2017年间于本院就诊并经病理确诊为结肠癌的患者121例,分析其P53、nm23的表达及与临床病理特征的相关性。结果 p53在肿瘤组织中的阳性率(76.03%)显著高于癌旁组织(4.96%)(P<0.05),而nm23的表达在肿瘤组织(80.17%)和癌旁组织(86.12%)中无明显差异(P>0.05)。p53的阳性率随着肿瘤分化程度的减低逐渐增高,最高达92.50%,组间差异具有统计学意义(P>0.05);nm23则相反,高分化组织的nm23表达阳性率最高,为86.84%。在有转移的患者的肿瘤组织中,p53的表达明显上升,nm23的表达明显下降(P<0.05)。结果肿瘤组织中p53蛋白过量表达提示肿瘤恶性程度高,转移可能大,nm23蛋白在组织分化程度高的组中和未发生淋巴结转移组中高表达,与抑制肿瘤生长相关,两者均可以作为判断结直肠癌预后效果的重要参考指标之一。
Objective To investigate the expression of P53 and nm23 in colon cancer and its relationship with clinicopathological features. Methods 121 patients with colorectal cancer were enrolled in this study from2013 to 2017. The expression of P53 and nm23 in colon cancer and its relationship with clinicopathological features were analyzed. Results The positive rate of p53 in tumor tissue( 76. 03%) was significantly higher than that in adjacent tissues( 4. 96%)( P < 0. 05),while there was no statistical difference in the expression of nm23 between tumor tissue( 80. 17%) and the adjacent tissue( 86. 12%) The positive rate of p53 was increased by 92. 50% with the decrease of tumor differentiation,while the expression of p53 was increased by86. 84% with the increase of tumor differentiation,the differences had statistical significance( P > 0. 05). In the patients with metastases,the expression of p53 was significantly increased while the expression of nm23 was significantly decreased( P < 0. 05). Conclusion The overexpression of p53 protein in tumor tissue indicates that the tumor could be highly malignant and the nm23 protein is highly expressed in the group with high degree of histological differentiation and no lymph node metastasis. It can be used as the one of the important reference indicators for prognostic effect of colorectal cancer.
Objective To investigate the expression of P53 and nm23 in colon cancer and its relationship with clinicopathological features. Methods 121 patients with colorectal cancer were enrolled in this study from2013 to 2017. The expression of P53 and nm23 in colon cancer and its relationship with clinicopathological features were analyzed. Results The positive rate of p53 in tumor tissue( 76. 03%) was significantly higher than that in adjacent tissues( 4. 96%)( P < 0. 05),while there was no statistical difference in the expression of nm23 between tumor tissue( 80. 17%) and the adjacent tissue( 86. 12%) The positive rate of p53 was increased by 92. 50% with the decrease of tumor differentiation,while the expression of p53 was increased by86. 84% with the increase of tumor differentiation,the differences had statistical significance( P > 0. 05). In the patients with metastases,the expression of p53 was significantly increased while the expression of nm23 was significantly decreased( P < 0. 05). Conclusion The overexpression of p53 protein in tumor tissue indicates that the tumor could be highly malignant and the nm23 protein is highly expressed in the group with high degree of histological differentiation and no lymph node metastasis. It can be used as the one of the important reference indicators for prognostic effect of colorectal cancer.
引文
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[18]徐健,项洪刚,易伟,等.nm23和MMP-9表达与结肠癌淋巴结转移比率相关性研究[J].山东医药,2012,52(44):29-30,34.
[19]王莉,任秀英,辛华,等.大肠癌中P53、nm23-H1和MMP-9基因的表达[J].黑龙江医药科学,2016,39(6):42-43.
[20]李鑫,龙汉安,肖秀丽,等.D2-40与nm23在结直肠癌中的表达及临床病理意义的探讨[J].泸州医学院学报,2014,37(5):456-460.
[2]康清杰,向征.结肠癌筛查和诊疗的研究进展[J].重庆医学,2015,44(28):4001-4003.
[3]邓婷,巴一.左右半结肠癌差异性研究进展[J].中国肿瘤临床,2015,42(13):684-688.
[4]王贲士,乔录新,石英,等.p53凋亡刺激蛋白2抑制奥沙利铂诱导的结肠癌细胞自噬并促进凋亡[J].细胞与分子免疫学杂志,2015,31(7):898-904.
[5]侯卫东,胡学信,刘艳峰.甲状腺乳头状癌中Cyclin D1表达和BRAF(V600 E)突变的相关性及意义[J].临床与实验病理学杂志,2014,30(12):1386-1390.
[6]BEESLEY M F,MCLAREN K M.Cytokeratin 19 and galectin-3immunohistochemistry in the differential diagnosis of solitary thyroid nodules[J].Histopathology,2002,41(3):236-243.
[7]王五艺,程勇,万林.AGEs通过JAK2/STAT3信号通路诱导结肠癌SW480细胞增殖、侵袭及上皮间质转化[D].第三军医大学学报,(2017-10-19).
[8]齐丽莎.Wnt/β-catenin通路在结肠癌血管生成拟态形成中的作用[D].天津医科大学,2012.
[9]隋华,周利红,殷佩浩,等.JNK信号通路介导MDR1/P-gp调控人结肠癌多药耐药[J].世界华人消化杂志,2011,19(9):892-898.
[10]多玥荷,孙莉娜,应森林,等.西黄丸通过ERK/MAPK信号通路对人结肠癌裸鼠移植瘤的影响[J].中华中医药杂志,2013,28(10):3055-3058.
[11]张巨彪,苏秀兰,欧阳晓晖.联合检测血清肿瘤标志物对结肠癌的诊断价值[J].医学综述,2015,21(20):3800-3801.
[12]黄媛,林莉,杨倩琳,等.结肠癌患者术前外周血中性粒细胞/淋巴细胞比值与预后的关系[J].检验医学与临床,2016,13(7):923-924,928.
[13]LU X,LIU D P,XU Y.The gain of function of p53 cancer mutant in promoting mammary tumorigenesis[J].Oncogene,2013,32(23):2900-2906.
[14]王晓博,贾喜花,郭星.STK15、P53蛋白在结肠癌中的表达及其临床意义[J].中国热带医学,2015,15(1):104-106.
[15]马琳园,隋御,马璐,等.MMS2和P53基因对结肠癌细胞增殖与凋亡的影响[J].中国免疫学杂志,2016,32(4):513-518.
[16]黄小杏,黄世勇,谢伟.150例结直肠癌浸润深度与淋巴结转移情况的临床病理研究[J].中国当代医药,2013,20(33):4-6,10.
[17]黄永穗,彭荣华,赵云燕.64例结肠癌患者的螺旋CT检测报告分析[J].中南医学科学杂志,2015,43(4):424-426.
[18]徐健,项洪刚,易伟,等.nm23和MMP-9表达与结肠癌淋巴结转移比率相关性研究[J].山东医药,2012,52(44):29-30,34.
[19]王莉,任秀英,辛华,等.大肠癌中P53、nm23-H1和MMP-9基因的表达[J].黑龙江医药科学,2016,39(6):42-43.
[20]李鑫,龙汉安,肖秀丽,等.D2-40与nm23在结直肠癌中的表达及临床病理意义的探讨[J].泸州医学院学报,2014,37(5):456-460.