温经汤不同煎煮方法比较研究
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摘要
目的:比较温经汤传统煎煮方法和现代医疗机构煎煮方法所得汤剂的质量差异,为温经汤的临床应用提供科学依据。方法:采用高效液相色谱法测定两种方法所得汤剂中芍药内酯苷、芍药苷、β-蜕皮甾酮、甘草苷、阿魏酸、甘草素、肉桂酸、桂皮醛、丹皮酚、甘草酸铵的含量,并对两种方法所得汤剂的总皂苷和浸膏率进行比较。结果:两种方法所得的汤剂中,芍药内酯苷、芍药苷、甘草苷、甘草素、肉桂酸、甘草酸铵含量差异具有统计学意义(P<0.05),β-蜕皮甾酮、阿魏酸、桂皮醛、丹皮酚、总皂苷、浸膏率差异均无统计学意义(P> 0.05)。结论:两种煎煮方法所得的温经汤质量存在差异,医疗机构煎煮法更具科学性。
Objective:To compare the qualify difference between Wenjing Decoction prepared by traditional decoding method and the decocting method of modern hospitals,so as to provide a scientific basis for the clinical application of Wenjing Decoction. Methods:High performance liquid chromatography(HPLC) was used to measure the contents of paeonoflavone glycoside,paeoniflorin,β-ecnesterone,liquiritin,ferulic acid,glycyrrhizin,cinnamic acid,cinnamaldehyde,paeonol,ammonium glycyrrhizinate in decoction prepared by two decoction methods. In addition,the content of the total saponins and the extract ratio were calculated. Results:There were significant differences in the contents of paeonoflavone plycoside,paeoniflorin,liquiritin,glycyrrhizin,cinnamic acid and ammonium glycyrrhizinate of the decoctions prepared by the two methods. However,there were no significant differences in the contents of β-ecdysterone,ferulic acid,cinnamaldehyde,paeonol,total saponins,and extract ratio of the decoctions prepared by the two methods. Conclusion:There are differences in the quality of Wenjing Decoction prepared by the two decoction methods. And the decoction method of modern hospital is more scientific.
Objective:To compare the qualify difference between Wenjing Decoction prepared by traditional decoding method and the decocting method of modern hospitals,so as to provide a scientific basis for the clinical application of Wenjing Decoction. Methods:High performance liquid chromatography(HPLC) was used to measure the contents of paeonoflavone glycoside,paeoniflorin,β-ecnesterone,liquiritin,ferulic acid,glycyrrhizin,cinnamic acid,cinnamaldehyde,paeonol,ammonium glycyrrhizinate in decoction prepared by two decoction methods. In addition,the content of the total saponins and the extract ratio were calculated. Results:There were significant differences in the contents of paeonoflavone plycoside,paeoniflorin,liquiritin,glycyrrhizin,cinnamic acid and ammonium glycyrrhizinate of the decoctions prepared by the two methods. However,there were no significant differences in the contents of β-ecdysterone,ferulic acid,cinnamaldehyde,paeonol,total saponins,and extract ratio of the decoctions prepared by the two methods. Conclusion:There are differences in the quality of Wenjing Decoction prepared by the two decoction methods. And the decoction method of modern hospital is more scientific.
引文
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[21]徐楠杰,郭月英,李铣.蜕皮甾酮的药理作用研究进展[J].沈阳药科大学学报,1997,14(4):69-71.
[2]林渊,周良良,吴水生.对中药汤剂剂型改革研究的思考[J].中国实验方剂学杂志,2011,17(5):264-266.
[3]徐秋香,郁红礼,吴皓,等.四君子汤传统煎煮规范化研究[J].中成药,2013,35(6):1325-1329.
[4]徐灵胎.医学源流论[M].北京:中国中医药出版社,2006:50.
[5]唐卓,刘宇新.温经汤治疗原发性寒凝血瘀型痛经的疗效观察80例[J].中医临床研究,2015,7(31):95-97.
[6]朱兰,林宝杏,林笑治,等.良方温经汤治疗子宫内膜异位症临床研究[J].中国中西医结合杂志,2000,20(9):696-697.
[7]单润琴.良方温经汤治疗子宫腺肌病31例[J].中医药临床杂志,2014,26(2):219-220.
[8]马佳维,叶明,李荣群.《金匮要略》与《妇人大全良方》温经汤之异同[J].陕西中医药大学学报,2016,39(4):82-84.
[9]陈自明.妇人大全良方[M].王咪咪,整理.北京:人民卫生出版社,2006:62
[10]袁冰,石东平,宋延青.略论宋代的煮散[J].中华中医药杂志,2005,20(10):585-587.
[11]邢丹,贺莹,郑虎占.从《太平惠民和剂局方》论中药煮散技术规范[J].中国临床医生,2012,40(11):73-75.
[12]邵长森,张国青,林桂涛.基于标准汤剂参比的炙甘草汤颗粒提取工艺的研究[J].时珍国医国药,2018,29(2):330-333.
[13]胡益勇,徐晓玉.阿魏酸的化学和药理研究进展[J].中成药,2006,28(2):253-255.
[14]刘娟,蒲忠慧,彭成,等.川芎中总生物碱、总酚酸和总挥发油对大鼠离体子宫平滑肌收缩活动的抑制作用[J].中国药房,2018,29(5):621-624.
[15]王成龙.基于白芍养血柔肝功效的芍药苷、芍药内酯苷药理作用研究[D].北京:北京中医药大学,2017.
[16]朱映黎,张建军,王景霞,等.芍药内酯苷和芍药苷对环磷酰胺致血虚小鼠的补血作用及对GM-CSF,IL-3,TNF-α影响的比较研究[J].中国中药杂志,2015,40(2):330-333.
[17]张利青,张占刚,付岩,等.桂皮醛药理作用的研究进展[J].中国中药杂志,2015,40(23):4568-4572.
[18]吴修富.肉桂提取物的主要化学成分及药理活性研究进展[J].中国药房,2015,26(24):3454-3456.
[19] KOO YK,KIM JM,KOO JY,et al. Platelet anti-aggreis-gatory and blood anti-coagulant effects of compounds ol-ated from Paeonia lactiflora and Paeonia suffruticosa[J].Pkarmazie,2010,65(8):624-628.
[20]张明发,沈雅琴.甘草及其活性成分对生殖系统药理作用研究进展[J].药物评价研究,2014,37(4):367-374.
[21]徐楠杰,郭月英,李铣.蜕皮甾酮的药理作用研究进展[J].沈阳药科大学学报,1997,14(4):69-71.