留兰香总黄酮含量测定方法的建立
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摘要
目的:建立以芦丁为对照品的留兰香(Herba Mentha spicata)总黄酮含量测定方法。方法:通过紫外扫描比较了芦丁对照品和留兰香提取物在亚硝酸钠-硝酸铝-氢氧化钠和三氯化铝2种显色体系下的吸收特性,确定一种检测方法作为留兰香总黄酮的测定较优方法。结果:亚硝酸钠-硝酸铝-氢氧化钠显色法更适合留兰香总黄酮含量的测定。标准曲线回归方程y=13.291x-0.011 5(R=0.999 8),芦丁的浓度和吸光度在8.008～48.050μg/mL范围内线性关系良好,平均加样回收率为97.4%,RSD为2.5%,重现性、稳定性和精密度均较好。结论:亚硝酸钠-硝酸铝-氢氧化钠显色法简便、快速、准确,可作为留兰香中总黄酮含量测定方法。
Objective:This study was designed to develop a quantitative determination method of the total flavonoids from Herba Mentha spicata using rutin as a standard substance for further develpoment and utilization. Methods:Confirming which was the best detection method as determination of total flavonoid from Herba Mentha spicata,the rutin and Herba Mentha spicata extract(total flavonoids)was comparative analyzed by UV scanning method with NaNO_2-A l(NO_3)_3-NaOH chromogenic system and AlCl_3 chromogenic system,respectively. Results:The method of NaNO_2-Al(NO_3)_3-NaOH chromogenic system was accurate,with good precision,stability and repeatability for the determination of total flavonoids from Herba Mentha spicata. It was confirmed that the rutin had a linear relationship with absorbance in the range of 8.008 μg/ml～48.05 μg/ml(y=13.291 x-0.0115,R=0.999 8).The average recovery was 99.71%(RSD =1.32%). The average recovery was 97.4% with RSD of 2.5%. Conclusion:This method of NaNO_2-Al(NO_3)_3-NaOH chromogenic system was easy to operate,fast and accurate,and it is suitable to detect the total flavonoids from Mentha spicata L.
Objective:This study was designed to develop a quantitative determination method of the total flavonoids from Herba Mentha spicata using rutin as a standard substance for further develpoment and utilization. Methods:Confirming which was the best detection method as determination of total flavonoid from Herba Mentha spicata,the rutin and Herba Mentha spicata extract(total flavonoids)was comparative analyzed by UV scanning method with NaNO_2-A l(NO_3)_3-NaOH chromogenic system and AlCl_3 chromogenic system,respectively. Results:The method of NaNO_2-Al(NO_3)_3-NaOH chromogenic system was accurate,with good precision,stability and repeatability for the determination of total flavonoids from Herba Mentha spicata. It was confirmed that the rutin had a linear relationship with absorbance in the range of 8.008 μg/ml～48.05 μg/ml(y=13.291 x-0.0115,R=0.999 8).The average recovery was 99.71%(RSD =1.32%). The average recovery was 97.4% with RSD of 2.5%. Conclusion:This method of NaNO_2-Al(NO_3)_3-NaOH chromogenic system was easy to operate,fast and accurate,and it is suitable to detect the total flavonoids from Mentha spicata L.
引文
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[14]李洪芹.白花鬼针草总黄酮提取工艺研究[J].山东化工,2017,13):30-32.
[15]郑杭生,李计萍,韩炜,等.紫外-可见分光光度法测定总黄酮含量的方法学考察要点[J].中成药,2008,30(9):1364-1365.
[16]丁明玉,赵纪萍,李擎阳.贯叶金丝桃提取物中总黄酮的测定方法[J].分析试验室,2001,20(6):45-47.
[17]李丹,程天印,懂振生.山莓叶总黄酮含量测定方法的建立[J].中兽医医药杂志,2016,35(6):38-41.
[18]包强,刘丽梅,王延玲,等.中药材有效部位总黄酮含量测定方法研究概述[J].中国中医药信息杂志,2018,25(4):136-140.
[2]王桂红,张群,谭永霞,等. HPLC测定留兰香中橙皮苷和迷迭香酸的含量[J].中国实验方剂学杂志,2016,22(6):38-41.
[3]李葆莉.实用中草药彩色图鉴(上)[M].北京:北京中医古籍出版社,2016:30-31.
[4]韩卫丽.留兰香提取物抗菌抗病毒作用研究[D].郑州:河南农业大学,2010.
[5] Ansari K,Goodarznia I.Optimization of supercritical carbon dioxide extraction of essential oil from spearmint(Mentha spicata L.)leaves by using Taguchi methodology[J].The Journal of Supercritical Fluids,2012,67:123-130.
[6] Kumar S, Pandey A K. Chemistry and biological activities of flavonoids:an overview[J]. The Scientific World Journal,2013,2013:1-16.
[7]郝瑞芬,贾会玲,钱骅,等.不同生长期留兰香精油含量与品质评价[J].中国食品添加剂,2017,(9):55-60.
[8]吕国红,钟晓凌,李良,等.枳椇子总黄酮测定方法的比较分析[J].湖北工业大学学报,2009,24(1):394-396.
[9]池玉梅,居羚,邓海山,等.分光光度测定总黄酮法的适用性[J].分析化学,2010,38(6):893-896.
[10] Bimakr M,Rahman R A,Taip F S,et al. Comparison of different extraction methods for the extraction of major bioactive flavonoid compounds from spearmint(Mentha spicata L.)leaves[J]. Food and Bioproducts Processing,2011,89(1):67-72.
[11]宋妍.留兰香水溶性部分化学成分的研究[D].沈阳:沈阳药科大学,2008.
[12] Bimakr M,Rahman R A,Ganjloo A,et al.Optimization of supercritical carbon dioxide extraction of bioactive flavonoid compounds from spearmint(Mentha spicata L.)leaves by using response surface methodology[J]. Food and Bioprocess Technology,2012,5(3):912-920.
[13]房英娟,林珠灿,郭素华.不同产地养心草中总黄酮的含量测定[J].亚太传统医药,2013,9(1):31-32.
[14]李洪芹.白花鬼针草总黄酮提取工艺研究[J].山东化工,2017,13):30-32.
[15]郑杭生,李计萍,韩炜,等.紫外-可见分光光度法测定总黄酮含量的方法学考察要点[J].中成药,2008,30(9):1364-1365.
[16]丁明玉,赵纪萍,李擎阳.贯叶金丝桃提取物中总黄酮的测定方法[J].分析试验室,2001,20(6):45-47.
[17]李丹,程天印,懂振生.山莓叶总黄酮含量测定方法的建立[J].中兽医医药杂志,2016,35(6):38-41.
[18]包强,刘丽梅,王延玲,等.中药材有效部位总黄酮含量测定方法研究概述[J].中国中医药信息杂志,2018,25(4):136-140.