游离棉酚降解产物四甲氧基棉酚致小鼠精子畸形效应的生殖毒性研究
|
摘要
目的研究游离棉酚降解产物四甲氧基棉酚的生殖毒性。方法采用小鼠精子畸变实验,将60只小鼠随机分为5组,空白组(40mg/kg,超纯水)、阳性对照组(40mg/kg,环磷酰胺)、四甲氧基棉酚的高(180mg/kg)、中(90mg/kg)和低(45mg/kg)剂量组,每组小鼠灌胃体积(20mL/kg体质量),每天1次,连续给药5d。取小鼠副睾制成精子涂片观察和计算各组小鼠精子畸形率。结果与正常小鼠精子相比,四甲氧基棉酚可导致小鼠精子形成香蕉型、胖头型和双尾型畸形类型。与空白组相比,阳性对照组小鼠精子畸形率为(8.20±0.31)%,四甲氧基棉酚高剂量组小鼠精子畸形率为(4.40±0.51)%、中剂量组小鼠精子畸形率为(3.00±0.33)%、低剂量组小鼠精子畸形率为(1.80±0.44)%,差异有统计学意义(P<0.05)。结论游离棉酚降解产物四甲氧基棉酚可导致小鼠精子畸形,具有生殖毒性。
Objective To investigate the reproductive toxicity of the free gossypol degradation product tetramethoxy gossypol.Methods Sperm aberration was tested in mice.60 mice were randomly divided into 5 groups:blank group(40 mg/kg,ultrapure water),positive control group(40 mg/kg,cyclophosphamide),and tetramethoxy cotton in the high(180 mg/kg),middle(90 mg/kg),and low(45 mg/kg)dose groups,the volume of each group of mice(20 mL/kg.BW)was administered once a day,continuously drug for 5 days.The mice secondary testis was taken to make sperm smears observation and the sperm deformity rate was calculated by SPSS 17.0.Results Compared to normal sperm,tetramethoxy gossypol results in the formation of spermatozoa in to styles of banana,fat head and twin tails.Compared with the blank group,the rate of sperm abnormality in the positive control group was(8.2±0.31)%,the sperm deformity rate of the high dose group of tetramethoxy gossypol was(4.4±0.51)%,the sperm abnormality rate in mice was(3.0±0.33)%in the medium-dose group and(1.8±0.44)%in the low-dose group and the difference was statistically significant(P <0.05).Conclusion Tetramethoxyl gossypol as the degradation products of free gossypol can cause sperm deformity in mice,and its toxicity increases with the increase of concentration,and it has reproductive toxicity.
Objective To investigate the reproductive toxicity of the free gossypol degradation product tetramethoxy gossypol.Methods Sperm aberration was tested in mice.60 mice were randomly divided into 5 groups:blank group(40 mg/kg,ultrapure water),positive control group(40 mg/kg,cyclophosphamide),and tetramethoxy cotton in the high(180 mg/kg),middle(90 mg/kg),and low(45 mg/kg)dose groups,the volume of each group of mice(20 mL/kg.BW)was administered once a day,continuously drug for 5 days.The mice secondary testis was taken to make sperm smears observation and the sperm deformity rate was calculated by SPSS 17.0.Results Compared to normal sperm,tetramethoxy gossypol results in the formation of spermatozoa in to styles of banana,fat head and twin tails.Compared with the blank group,the rate of sperm abnormality in the positive control group was(8.2±0.31)%,the sperm deformity rate of the high dose group of tetramethoxy gossypol was(4.4±0.51)%,the sperm abnormality rate in mice was(3.0±0.33)%in the medium-dose group and(1.8±0.44)%in the low-dose group and the difference was statistically significant(P <0.05).Conclusion Tetramethoxyl gossypol as the degradation products of free gossypol can cause sperm deformity in mice,and its toxicity increases with the increase of concentration,and it has reproductive toxicity.
引文
[1]印南日,李培武,周海燕,等.我国食用棉籽油质量安全[J].中国农业科技导报,2013,15(4):20-24.
[2]潘江贵.棉酚的特性及在棉籽油生产中的工艺控制[J].中国新技术新产品,2013,6(1):23-23.
[3]曾秋凤,柏鹏.棉酚在肉禽体内的毒性和残留及其营养对策的研究进展[J].动物营养学报,2013,25(5):917-922.
[4]王晓翠,武书庚,张海军,等.游离棉酚对鸡蛋品质的影响及其脱除方法研究进展[J].动物营养学报,2014,26(3):571-577.
[5]周晨忱,王晶,姚军,等.HPLC法考察棉籽油放置过程中游离棉酚稳定性[J].食品科技,2014,39(9):311-314.
[6]倪明,袁慧.棉酚对动物生殖毒性的研究进展[J].中国畜牧医兽,2008,35(4):144-145.
[7]GADELHA I C,FONSECA N B,OLORIS S C,et al.Gossypol toxicity from cottonseed products[J].The Sci World J,2014,16(3):16-19.
[8]武钰,闫明俞,耿文宁,等.棉籽油中游离棉酚脱毒评价新方法[J].中国测试,2015,43(4):52-56.
[9]MOKADEM M T.Alleviation of reproductive toxicity of gossypol using selenium supplementation in rams[J].J Animal Sci,2012,90(9):3274-3285.
[10]袁彦波,赵树臣,侯振中.棉酚对雄性小鼠的生殖毒性作用[J].中国兽医杂志,2014,51(7):83-85.
[11]戴成吉.镉对小鼠精母细胞凋亡及附睾内精子质量的影响[D].上海:上海交通大学,2010.
[2]潘江贵.棉酚的特性及在棉籽油生产中的工艺控制[J].中国新技术新产品,2013,6(1):23-23.
[3]曾秋凤,柏鹏.棉酚在肉禽体内的毒性和残留及其营养对策的研究进展[J].动物营养学报,2013,25(5):917-922.
[4]王晓翠,武书庚,张海军,等.游离棉酚对鸡蛋品质的影响及其脱除方法研究进展[J].动物营养学报,2014,26(3):571-577.
[5]周晨忱,王晶,姚军,等.HPLC法考察棉籽油放置过程中游离棉酚稳定性[J].食品科技,2014,39(9):311-314.
[6]倪明,袁慧.棉酚对动物生殖毒性的研究进展[J].中国畜牧医兽,2008,35(4):144-145.
[7]GADELHA I C,FONSECA N B,OLORIS S C,et al.Gossypol toxicity from cottonseed products[J].The Sci World J,2014,16(3):16-19.
[8]武钰,闫明俞,耿文宁,等.棉籽油中游离棉酚脱毒评价新方法[J].中国测试,2015,43(4):52-56.
[9]MOKADEM M T.Alleviation of reproductive toxicity of gossypol using selenium supplementation in rams[J].J Animal Sci,2012,90(9):3274-3285.
[10]袁彦波,赵树臣,侯振中.棉酚对雄性小鼠的生殖毒性作用[J].中国兽医杂志,2014,51(7):83-85.
[11]戴成吉.镉对小鼠精母细胞凋亡及附睾内精子质量的影响[D].上海:上海交通大学,2010.