甘肃省陇东南大豆孢囊线虫的发生和分布
|
摘要
为了解甘肃省陇东南地区大豆孢囊线虫病分布和发生程度,采用Z字形取样法,对甘肃省陇东南地区9个县(区)37个乡镇大豆孢囊线虫病的发生和分布进行调查,并根据形态特征和rDNA-ITS序列比对鉴定线虫种类。共采集土样411份,陇东地区大豆孢囊线虫检出率为87.2%,其中镇原、西峰、正宁、华池和环县的检出率最高,达100%,镇原孢囊发生量最大,平均孢囊数为11.4个/100 g土;陇南地区大豆孢囊线虫检出率为63.7%,成县和徽县分别为64.0%、63.0%,平均孢囊数分别为0.9和1.2个/100 g土。经形态学特征观测和rDNA-ITS序列比对,甘肃省陇东南采集到的孢囊线虫均鉴定为大豆孢囊线虫Heterodera glycines。
In order to understand the occurrence and distribution of soybean cyst nematode in southeast of Gansu Province, zigzag sampling method was used to investigate soybean cyst nematodes in 37 townships of 9 counties in southeastern Gansu. The species of nematodes were identified based on the morphological observation and rDNA-ITS sequence analysis. Totally, 411 samples of soil samples were collected. The results showed that the detection rate of soybean cyst nematodes in eastern Gansu was 87.2%, among which the detection rates in Zhenyuan, Xifeng, Zhengning, Huachi and Huan County were the highest, with the rates of 100%, while that in Qingcheng was the lowest by 39.3%. The highest density was found in Zhenyuan with the average cysts number of 11.4 per 100 g soil. The detection rate of soybean cyst nematodes in southern Gansu was 63.7% and those in Chengxian and Huixian were 64.0% and 63.0%, respectively, and average densities of nematodes were 0.9 and 1.2 per 100 g soil, respectively. Morphological observation and rDNA-ITS sequence analysis showed that the cyst nematodes collected in southeastern Gansu were identified as Heterodera glycines.
In order to understand the occurrence and distribution of soybean cyst nematode in southeast of Gansu Province, zigzag sampling method was used to investigate soybean cyst nematodes in 37 townships of 9 counties in southeastern Gansu. The species of nematodes were identified based on the morphological observation and rDNA-ITS sequence analysis. Totally, 411 samples of soil samples were collected. The results showed that the detection rate of soybean cyst nematodes in eastern Gansu was 87.2%, among which the detection rates in Zhenyuan, Xifeng, Zhengning, Huachi and Huan County were the highest, with the rates of 100%, while that in Qingcheng was the lowest by 39.3%. The highest density was found in Zhenyuan with the average cysts number of 11.4 per 100 g soil. The detection rate of soybean cyst nematodes in southern Gansu was 63.7% and those in Chengxian and Huixian were 64.0% and 63.0%, respectively, and average densities of nematodes were 0.9 and 1.2 per 100 g soil, respectively. Morphological observation and rDNA-ITS sequence analysis showed that the cyst nematodes collected in southeastern Gansu were identified as Heterodera glycines.
引文
[1] 陈双.大豆孢囊线虫生理小种鉴定及其致病性研究[D].哈尔滨:黑龙江大学,2015.
[2] LIU Xingzhong,LI Jianqiang,ZHANG Dongsheng.History and status of soybean cyst nematode in China [J].International Journal of Nematology,1997,7(1):18-25.
[3] 马朝旺,段玉玺,陈立杰,等.辽宁省大豆孢囊线虫生理分化研究[J].大豆科学,2009,28(2):285-289.
[4] MAAFI Z,SALATI T M,RIGGS R D.Distribution,population density,race and type determination of soybean cyst nematode,Heterodera glycines,in Iran [J].Nematology,2008,10(6):919-924.
[5] 刘维志.植物病原线虫学[M].北京:中国农业出版社,2000:281-294.
[6] 刘维志,刘畔,段玉玺,等.中国大豆孢囊线虫的形态学观察[J].沈阳农业大学学报,1994,25(2):164-167.
[7] ZHENG Junye,ZHANG Youni,LI Xianpei.First report of the soybean cyst nematode,Heterodera glycines,on soybean in Zhejiang,Eastern China [J].Plant Disease,2009,93(3):319.
[8] 李克梅,日孜枉古丽,董艳秋.新疆大豆孢囊线虫病的初步研究[J].植物保护,2014,40(2):132-134.
[9] 张玉华.大豆孢囊线虫病的危害与防治[J].大豆通报,1995(5):26-27.
[10] 王守义.抗大豆孢囊线虫病种质资源的筛选与创新[J].大豆通报,1995(4):13-14.
[11] 叶文兴,彭德良,黄文坤,等.甘肃首次发现大豆孢囊线虫(Heterodera glycines)[C]//中国植物保护学会.病虫害绿色防控与农产品质量安全——中国植物保护学会2015年学术年会论文集,2015:1.
[12] PENG Deliang,PENG Huan,WU Duqing,et al.First report of soybean cyst nematode (Heterodera glycines) on soybean from Gansu and Ningxia China [J].Plant Disease,2015,100(1):229.
[13] 李惠霞,刘永刚,朱锐东,等.甘肃省小麦禾谷孢囊线虫的发生及分布[J].植物保护,2016,43(3):170-174.
[14] 冯志新.植物线虫学[M].北京:中国农业出版社,2001:132-135,176.
[15] 王明祖,彭德良,武学勤.小麦孢囊线虫病的研究Ⅰ:病原鉴定[J].华中农业大学学报,1991,10(4):352-356.
[16] 彭德良,SUBBTIN S,MOENS M.小麦禾谷孢囊线虫(Heterodera avenae)的核糖体基因(rDNA)限制性片段长度多态性研究[J].植物病理学报,2003,33(4):323-329.
[17] SZALANSKI A,SUI D D,HARRIS T S,et al.Identification of cyst nematodes of agronomic and regulatory concern with PCR-RFLP of ITS1 [J].Journal of Nematology,1997,29(4):255-267.
[18] PERRY R N,MOENS M.Plant nematology [M].简恒,译.北京:中国农业大学出版社,2011:97-98.
[19] 宋美静.我国大豆孢囊线虫群体致病性分化及其辅助鉴别寄主的发掘[D].沈阳:沈阳农业大学,2016.
[20] 张俊立,彭德良,曹克强.河北省大豆孢囊线虫分子鉴定及其分布[J].植物保护,2005,31(1):40-43.
[21] 宋美静,朱晓峰,王东,等.我国大豆主产区大豆孢囊线虫群体分布及致病性分化研究[J].大豆科学,2016,35(4):630-636.
[22] 孙玉秋,徐艳丽,李春杰,等.黑龙江省大豆孢囊线虫种群分布和卵密度研究[J].大豆科学,2011,30(2):250-253.
[23] 龙海波,陈文,裴月令,等.贵州省大豆孢囊线虫的发生与分布[J].植物保护,2017,43(6):173-176.
[24] 孙晶双,郑雅楠,段玉玺,等.温度、光照和土壤含水量对大豆孢囊线虫休眠卵孵化的影响[J].大豆科学,2009,28(4):690-692.
[25] 刘冰.大豆孢囊线虫病的发生条件及防治措施[J].现代农业科技,2011(3):186.
[26] RIGGS R D,SCHMITT D P,Noel G R.Variability in race tests with Heterodera glycines[J].Journal of Nematology,1988,20:565- 572.
[2] LIU Xingzhong,LI Jianqiang,ZHANG Dongsheng.History and status of soybean cyst nematode in China [J].International Journal of Nematology,1997,7(1):18-25.
[3] 马朝旺,段玉玺,陈立杰,等.辽宁省大豆孢囊线虫生理分化研究[J].大豆科学,2009,28(2):285-289.
[4] MAAFI Z,SALATI T M,RIGGS R D.Distribution,population density,race and type determination of soybean cyst nematode,Heterodera glycines,in Iran [J].Nematology,2008,10(6):919-924.
[5] 刘维志.植物病原线虫学[M].北京:中国农业出版社,2000:281-294.
[6] 刘维志,刘畔,段玉玺,等.中国大豆孢囊线虫的形态学观察[J].沈阳农业大学学报,1994,25(2):164-167.
[7] ZHENG Junye,ZHANG Youni,LI Xianpei.First report of the soybean cyst nematode,Heterodera glycines,on soybean in Zhejiang,Eastern China [J].Plant Disease,2009,93(3):319.
[8] 李克梅,日孜枉古丽,董艳秋.新疆大豆孢囊线虫病的初步研究[J].植物保护,2014,40(2):132-134.
[9] 张玉华.大豆孢囊线虫病的危害与防治[J].大豆通报,1995(5):26-27.
[10] 王守义.抗大豆孢囊线虫病种质资源的筛选与创新[J].大豆通报,1995(4):13-14.
[11] 叶文兴,彭德良,黄文坤,等.甘肃首次发现大豆孢囊线虫(Heterodera glycines)[C]//中国植物保护学会.病虫害绿色防控与农产品质量安全——中国植物保护学会2015年学术年会论文集,2015:1.
[12] PENG Deliang,PENG Huan,WU Duqing,et al.First report of soybean cyst nematode (Heterodera glycines) on soybean from Gansu and Ningxia China [J].Plant Disease,2015,100(1):229.
[13] 李惠霞,刘永刚,朱锐东,等.甘肃省小麦禾谷孢囊线虫的发生及分布[J].植物保护,2016,43(3):170-174.
[14] 冯志新.植物线虫学[M].北京:中国农业出版社,2001:132-135,176.
[15] 王明祖,彭德良,武学勤.小麦孢囊线虫病的研究Ⅰ:病原鉴定[J].华中农业大学学报,1991,10(4):352-356.
[16] 彭德良,SUBBTIN S,MOENS M.小麦禾谷孢囊线虫(Heterodera avenae)的核糖体基因(rDNA)限制性片段长度多态性研究[J].植物病理学报,2003,33(4):323-329.
[17] SZALANSKI A,SUI D D,HARRIS T S,et al.Identification of cyst nematodes of agronomic and regulatory concern with PCR-RFLP of ITS1 [J].Journal of Nematology,1997,29(4):255-267.
[18] PERRY R N,MOENS M.Plant nematology [M].简恒,译.北京:中国农业大学出版社,2011:97-98.
[19] 宋美静.我国大豆孢囊线虫群体致病性分化及其辅助鉴别寄主的发掘[D].沈阳:沈阳农业大学,2016.
[20] 张俊立,彭德良,曹克强.河北省大豆孢囊线虫分子鉴定及其分布[J].植物保护,2005,31(1):40-43.
[21] 宋美静,朱晓峰,王东,等.我国大豆主产区大豆孢囊线虫群体分布及致病性分化研究[J].大豆科学,2016,35(4):630-636.
[22] 孙玉秋,徐艳丽,李春杰,等.黑龙江省大豆孢囊线虫种群分布和卵密度研究[J].大豆科学,2011,30(2):250-253.
[23] 龙海波,陈文,裴月令,等.贵州省大豆孢囊线虫的发生与分布[J].植物保护,2017,43(6):173-176.
[24] 孙晶双,郑雅楠,段玉玺,等.温度、光照和土壤含水量对大豆孢囊线虫休眠卵孵化的影响[J].大豆科学,2009,28(4):690-692.
[25] 刘冰.大豆孢囊线虫病的发生条件及防治措施[J].现代农业科技,2011(3):186.
[26] RIGGS R D,SCHMITT D P,Noel G R.Variability in race tests with Heterodera glycines[J].Journal of Nematology,1988,20:565- 572.