4种提取工艺制备溃结康栓对溃疡性结肠炎大鼠的治疗作用
|
摘要
目的研究4种提取工艺制备的溃结康栓对2,4,6-三硝基苯磺酸(TNBS)诱导溃疡性结肠炎(UC)大鼠的治疗作用。方法 SD雄性大鼠随机分为正常对照组、模型组、美沙拉秦栓组和4种提取工艺制备的溃结康栓组共7组,每组10只,用TNBS法复制UC大鼠模型,各给药组给予相应栓剂治疗,连续14 d。以各组实验大鼠疾病活动指数(DAI)、结肠黏膜损伤度、组织病理学改变、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、白介素-4(IL-4)等为指标,对其治疗疗效进行评价。结果模型组大鼠DAI评分、结肠黏膜损伤评分,血清中TNF-α、IL-1β及IL-4水平与正常对照组比较差异有统计学意义(P<0.01),美沙拉秦栓组、各工艺溃结康栓组DAI评分、结肠黏膜损伤评分显著降低,TNF-α、IL-1β及IL-4水平与模型组比较有统计学意义(P<0.05);美沙拉秦栓组与工艺Ⅰ溃结康栓组、工艺Ⅳ溃结康栓组DAI评分,TNF-α、IL-1β及IL-4水平比较无统计学意义。结论 4种提取工艺制备的溃结康栓均对TNBS诱导的UC模型大鼠有治疗作用,其中以提取工艺Ⅳ制备的溃结康栓疗效尤为显著,可为溃结康栓制剂研究提供工艺基础。
Objective To study effects of Kuijiekang suppository with four different processes on rats with ulcerative colitis induced by TNBS. Methods SD male rats were randomly divided into control group, model group,positive control group and four groups of Kuijiekang suppository with four different processes,10 rats per group. Rats model of ulcerative colitis set up by rectally administration of TNBS, except control group、model group, were given the appropriate medication to treat for 14 days. Take disease activity index, colon tissue injuries of scores, pathology,TNF-α, IL-1β, IL-4 as the detection indicator and evaluate the treatment. Results Compared with the control group,the model rats showed DAI significantly increased, the levels of TNF-α, IL-1βincreased(P<0.01), and IL-4 decreased(P<0.01). Compared with the model group, different processes and positive control group showed DAI significantly decreased(P <0.05), the levels of TNF-α, IL-1βdecreased(P <0.05), and IL-4(P <0.05) increased. Conclusion Kuijiekang suppositories with different processes rectly have effect on the ulcerative colitis induced by TNBS,especially in the first and the fourth process. Thus, the fourth process should be taken into consideration to conduct the next research.
Objective To study effects of Kuijiekang suppository with four different processes on rats with ulcerative colitis induced by TNBS. Methods SD male rats were randomly divided into control group, model group,positive control group and four groups of Kuijiekang suppository with four different processes,10 rats per group. Rats model of ulcerative colitis set up by rectally administration of TNBS, except control group、model group, were given the appropriate medication to treat for 14 days. Take disease activity index, colon tissue injuries of scores, pathology,TNF-α, IL-1β, IL-4 as the detection indicator and evaluate the treatment. Results Compared with the control group,the model rats showed DAI significantly increased, the levels of TNF-α, IL-1βincreased(P<0.01), and IL-4 decreased(P<0.01). Compared with the model group, different processes and positive control group showed DAI significantly decreased(P <0.05), the levels of TNF-α, IL-1βdecreased(P <0.05), and IL-4(P <0.05) increased. Conclusion Kuijiekang suppositories with different processes rectly have effect on the ulcerative colitis induced by TNBS,especially in the first and the fourth process. Thus, the fourth process should be taken into consideration to conduct the next research.
引文
[1]EADEN J A,ABRAMS K R,MAYBERRY J F.The risk of colorectal cancer in ulcerative colitis a meta-analysis[J].Gut,2001,50(4):526-535.
[2]RUTTER M D,SAUNDERS B P,WILKINSON K H,et al.Cancer surveillance in longstanding ulcerative colitis:endoscopic appearances help predict Cancer risk[J].Gut,2004,53(12):1813-1816.
[3]王桂娟.溃结康治疗溃疡性结肠炎的临床与实验研究[D].济南:山东中医药大学,2002.
[4]李婧.溃结康对TNBS诱导大鼠克罗恩病模型的治疗作用[D].天津:天津医科大学,2016.
[5]于培.溃结康对噁唑酮诱导溃疡性结肠炎大鼠模型的治疗作用[D].天津:天津医科大学,2016.
[6]MORRI J P,BECK P L,HERRIDEGE M S,et al.Hapteninduced Model of chronic infl ammation and ulceration in the rat colon[J].Gastroenterology,1989,96(3):795-803.
[7]KOETZNER L,GROVER G,BOULET J,et al.Plant-derived polysaccharide supplements inhibit dextran sulfate sodiuminduced colitis in the rat[J].Dig Dis Sci,2010,55(5):1278.
[8]LUK H H,KO J K,FUNG H S,et al.Delineation of the protective action of Zinc sulfate on ulcerative colitis in rats[J].Eur J Pharmacol,2002,443(1/3):197-204.
[9]D’ARIENZO A,MANGUSO F,ASTARITA C,et al.Allergy and mucosal eosinophil infi ltrate in ulcerative colitis[J].Scand J Gastroenterol,2000,35(6):624-631.
[10]FABIA R,WILLéN R,Ar'RAJAB A,et al.Acetic acidinduced colitis in the rat:a reproducible experimental model for acute ulcerative colitis[J].Eur Surg Res,1992,24(4):211.
[11]GUAN Q,MA Y,HILLMAN C L,et al.Targeting IL-12/IL-23 by employing a p40 peptide-based vaccine ameliorates TNBS-induced acute and chronic murine colitis[J].Mol Med,2011,17(7/8):646-656.
[12]赵曼,高峰.溃疡性结肠炎发病机制研究进展[J].现代生物医学进展,2010,10(16):3160-3165.
[2]RUTTER M D,SAUNDERS B P,WILKINSON K H,et al.Cancer surveillance in longstanding ulcerative colitis:endoscopic appearances help predict Cancer risk[J].Gut,2004,53(12):1813-1816.
[3]王桂娟.溃结康治疗溃疡性结肠炎的临床与实验研究[D].济南:山东中医药大学,2002.
[4]李婧.溃结康对TNBS诱导大鼠克罗恩病模型的治疗作用[D].天津:天津医科大学,2016.
[5]于培.溃结康对噁唑酮诱导溃疡性结肠炎大鼠模型的治疗作用[D].天津:天津医科大学,2016.
[6]MORRI J P,BECK P L,HERRIDEGE M S,et al.Hapteninduced Model of chronic infl ammation and ulceration in the rat colon[J].Gastroenterology,1989,96(3):795-803.
[7]KOETZNER L,GROVER G,BOULET J,et al.Plant-derived polysaccharide supplements inhibit dextran sulfate sodiuminduced colitis in the rat[J].Dig Dis Sci,2010,55(5):1278.
[8]LUK H H,KO J K,FUNG H S,et al.Delineation of the protective action of Zinc sulfate on ulcerative colitis in rats[J].Eur J Pharmacol,2002,443(1/3):197-204.
[9]D’ARIENZO A,MANGUSO F,ASTARITA C,et al.Allergy and mucosal eosinophil infi ltrate in ulcerative colitis[J].Scand J Gastroenterol,2000,35(6):624-631.
[10]FABIA R,WILLéN R,Ar'RAJAB A,et al.Acetic acidinduced colitis in the rat:a reproducible experimental model for acute ulcerative colitis[J].Eur Surg Res,1992,24(4):211.
[11]GUAN Q,MA Y,HILLMAN C L,et al.Targeting IL-12/IL-23 by employing a p40 peptide-based vaccine ameliorates TNBS-induced acute and chronic murine colitis[J].Mol Med,2011,17(7/8):646-656.
[12]赵曼,高峰.溃疡性结肠炎发病机制研究进展[J].现代生物医学进展,2010,10(16):3160-3165.