溃结康对三硝基苯磺酸诱导大鼠炎症性肠病的作用研究
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摘要
目的观察溃结康经灌肠给药对三硝基苯磺酸(trinitrobenzene sulfonic acid,TNBS)诱导的炎症性肠病动物模型的作用,以明确溃结康对炎症性肠病的治疗作用,为探索药物应用于克罗恩病治疗的可行性。方法采用TNBS灌肠的方法诱导大鼠溃疡性结肠炎模型,观察溃结康灌肠给药对大鼠疾病活动指数(DAI)评分、血清干扰素-γ(IFN-γ)、白介素-l(IL-1)、白介素-4(IL-4)、白介素-10(IL-10)和肠黏膜中谷氨酰胺(Glutamine,Gln)的含量的影响,并观察药物对大鼠结肠组织损伤评分以及病理学的影响。结果 TNBS诱导的结肠炎模型可引起动物体质量下降,肉眼血便或便潜血阳性,DAI评分增高,血清IFN-γ,IL-1升高,肠黏膜Gln降低,溃结康不同剂量组灌肠给药可改善TNBS引起的DAI评分增高以及降低血清IFN-γ、IL-1水平,升高肠黏膜Gln,缓解结肠炎组织学变化,其中尤以溃结康高剂量组作用更为明显。结论溃结康灌肠给药对缓解TNBS诱导结肠炎模型有一定治疗作用。
Objective To observe the effect of Kuijiekang on model induced by trinitrobenzene sulfonic acid, to examine its efficacy for inflammatory bowel disease, and to explore the feasibility of this medicine used for treatment of Cronh's disease. Methods Rat model of inflammatory bowel disease was set up by rectally administration of TNBS, and the effects of Kuijikang on indexes of DAI, IFN-γ, IL-1, and IL-4, as well as and the content of glutamine(Gln) in rats were determined. Results The rats with inflammatory bowel disease induced by TNBS have decreased body weight, macroscopical or microscopical hematochezia, increased DAI score, increased levels of IFN-γ and IL-1, and decreased Gln. Different doses of Kuijiekang could significantly improve the change of DAI score, lower the levels of IFN-γ and IL-1, raise the Gln in intestinal mucosa caused by TNBS, and relieve the histology change of colon inflammation, the highest dose with the most significant effect. Conclusion Kuijiekang rectlly administerd is effective for the colitis model induced by TNBS.
Objective To observe the effect of Kuijiekang on model induced by trinitrobenzene sulfonic acid, to examine its efficacy for inflammatory bowel disease, and to explore the feasibility of this medicine used for treatment of Cronh's disease. Methods Rat model of inflammatory bowel disease was set up by rectally administration of TNBS, and the effects of Kuijikang on indexes of DAI, IFN-γ, IL-1, and IL-4, as well as and the content of glutamine(Gln) in rats were determined. Results The rats with inflammatory bowel disease induced by TNBS have decreased body weight, macroscopical or microscopical hematochezia, increased DAI score, increased levels of IFN-γ and IL-1, and decreased Gln. Different doses of Kuijiekang could significantly improve the change of DAI score, lower the levels of IFN-γ and IL-1, raise the Gln in intestinal mucosa caused by TNBS, and relieve the histology change of colon inflammation, the highest dose with the most significant effect. Conclusion Kuijiekang rectlly administerd is effective for the colitis model induced by TNBS.
引文
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[9]孙敬平,马洪升.谷氨酰胺对肠黏膜细胞凋亡影响的研究进展[J].四川医学,2008,29(2):242-244.
[2]Brand S.Crohn’s disease:Th1,Th17 or both?The change of a paradigm:new immunological and genetic insights implicate Th17 cells in the pathogenesis of Crohn’s disease[J].Gut,2009,58(8):1152-1167.
[3]Morris J P,Beck P L,Herridege M S,et al.Hapten induced model of chronic inflammation and ulceration in the rat colon[J].Gastroenterology,1989,96(3):795-803.
[4]徐叔云.药理实验方法学[M].第三版.北京:人民卫生出版社,2001.
[5]孙晓萍,侯丽娟,王晓红,等.大鼠慢性溃疡性结肠炎模型的建立及操作规范探讨[J].天津中医药,2012,29(3):270-274.
[6]Guan Q,Ma Y,Hillman C L,et al.Targeting IL-12/IL-23by employing a p40 peptide-based vaccine ameliorates TNBS-induced acute and chronic murine colitis[J].Mol Med,2011,17(7-8):646-656.
[7]Fuss I J,Marth T,Neurath M F,et al.Anti-interleukin 12treatment regulates apoptosis of Th1 T cells in experimental colitis in mice[J].Gastroenterology,1999,117(5):1078-1088.
[8]路晓红,杨恩来,赵霞,等.溃疡性结肠炎免疫机制的研究进展[J].山西中医学院学报,2011,12(1):66-70.
[9]孙敬平,马洪升.谷氨酰胺对肠黏膜细胞凋亡影响的研究进展[J].四川医学,2008,29(2):242-244.