超临界二氧化碳萃取大别山五针松针叶挥发油及其成分分析
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摘要
采用超临界二氧化碳萃取技术(SFE-CO_2)提取大别山五针松针叶挥发油,并用GC-MS分析挥发油的化学成分,用峰面积归一法测定各化合物的相对含量。结果从超临界CO_2提取物中共鉴定出33种化合物,占总峰面积的81.70%,主要成分为萜类(36.65%)、醇类(22.96%)、烷烃类(11.33%)和酯类(10.76%),其中相对百分含量较高的有(-)-斯巴醇(11.55%),d-杜松烯(10.72%)、α-杜松醇(8.65%)、石竹烯(7.26%)和蒎烷(6.43%)等。
The volatile oil from Pinus dabeshanensis needles was extracted by supercritical CO_2 fluid(SFE-CO_2). The chemical components were separated and analyzed by gas chromatography-mass spectrometry(GC-MS). The results showed a total of 33 compounds were successfully identified by SFE-CO_2, representing 81.70% of the total compounds, of which terpenes(36.65%), alcohols(22.96%), alkanes(11.33%) and esters(10.76%)were the major constituents.(-)-spathulenol(11.55%), d-cadinene(10.72%), α-cadinol(8.65%), caryophyllene(7.26%) and 2,6,6-trimethylbicyclo [3.1.1]heptane(6.43%) were the main components.
The volatile oil from Pinus dabeshanensis needles was extracted by supercritical CO_2 fluid(SFE-CO_2). The chemical components were separated and analyzed by gas chromatography-mass spectrometry(GC-MS). The results showed a total of 33 compounds were successfully identified by SFE-CO_2, representing 81.70% of the total compounds, of which terpenes(36.65%), alcohols(22.96%), alkanes(11.33%) and esters(10.76%)were the major constituents.(-)-spathulenol(11.55%), d-cadinene(10.72%), α-cadinol(8.65%), caryophyllene(7.26%) and 2,6,6-trimethylbicyclo [3.1.1]heptane(6.43%) were the main components.
引文
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[11]田玉红,李梓,梁才. 拉雅松和细叶云南松松针挥发油的化学成分[J].中国实验方剂学杂志, 2012, 18(1):51-55.
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[2]项小燕, 张小平, 段仁燕, 等. 濒危植物大别山五针松母树林花粉传播规律[J]. 广西植物, 2014, 34(3):333-337.3.
[3]王雷宏, 黄庆丰, 蒲发光, 等. 天马自然保护区大别山五针松与群落中优势种的种间关系[J]. 长江流域资源与环境, 2014, 23(7): 960-965.
[4]项小燕, 吴甘霖, 段仁燕, 等. 大别山五针松种内和种间竞争[J]. 生态学报, 2015, 35(1):389-395.
[5]XIANG X Y, ZHANG Z X, DUAN R Y, et al. Genetic diversity and structure of Pinus dabeshanensis revealed by expressed sequence tag-simple sequence repeat (EST-SSR) markers [J]. Biochemical Systematics and Ecology, 2015, 61:70-77.
[6]杨燕, 杨茂发, 杨再华, 等. 云南松松针的挥发性化学成分[J]. 林业科学, 2009, 45(5):173-177.
[7]吴燕, 郭蕴斐, 卢山. 植物的防御性萜类挥发信号分子[J]. 植物生理学报, 2012, 48 (4): 311-317.
[8]孟昭军, 严善春, 徐伟. 长白落叶松8个家系挥发性化合物的比较分析[J]. 林业科学, 2008, 44(6):91-96.
[9]朴相勇,刘向前, 陆昌洙, 等. 红松叶挥发油成分的GC-MS分析[J]. 中草药, 2005, 36(12):1784.
[10]滕坤,张海丰,徐敏, 等. 赤松与长白赤松松针挥发油成分的GC-MS分析[J].药物分析杂志, 2011, 31(11):2121-2125.
[11]田玉红,李梓,梁才. 拉雅松和细叶云南松松针挥发油的化学成分[J].中国实验方剂学杂志, 2012, 18(1):51-55.
[12]KUROSE K, OKAMURA D, YATAGAI M. Composition of the essential oils from the leaves of nine Pinus species and the cones of three of Pinus species[J]. Flavour and Fragrance Journal, 2007, 22: 10.
[13]林立, 岑佳乐, 金华玫, 等. 五种柏科植物挥发油成分的GC-MS分析[J]. 广西植物, 2015, 35(4):580-585.