光学相干断层成像检测牙釉质表层脱矿与再矿化的实验研究
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摘要
目的利用光学相干断层成像技术(OCT)观察不同制剂对年轻恒牙釉脱矿和再矿化效果的影响。方法将正畸拔除的年轻恒前磨牙制成60个釉质块,随机均分为4组。每天进行如下处理:A组蒸馏水湿敷30min;B组含氟牙膏湿敷30min;C组护牙素湿敷30min;D组多乐氟湿敷30min,置于脱矿液1.5h,最后置于人工唾液中22h。每个步骤间用去离子水冲洗实验样品。实验周期重复30天。在处理前和第7、15、30天,使用扫频光源OCT完成对样本的扫描和成像,同时使用显微硬度计测量各样本表面的显微硬度。结果釉质脱矿深度:7天后,A组与其他各组相互比较,差异有统计学意义(P<0.05),B、C、D组组间比较差异无统计学意义(P>0.05)。30天后,C组和D组比较,差异无统计学意义(P>0.05),其余各组之间两两比较,差异均有统计学意义(P<0.05)。显微硬度测量:30天后,各处理组较处理前显微硬度值降低,A组显微硬度值最低,C组与D组显微硬度值高于B组,且差异有统计学意义(P<0.05)。C组和D组间釉质块显微硬度值差异无统计学意义(P>0.05)。结论 OCT可以较准确的显示釉质脱矿部位,在早期龋的诊断方面具有临床意义。护牙素和多乐氟均能有效的抑制牙釉质表面脱矿。
Objective To investigate the effect of different preparations on demineralization and remineralization of young permanent teeth by optical coherence tomography(OCT). Methods A total of 60 enamel blocks were prepared from young permanent premolars and randomly divided into 4 groups. The surface of each sample was applied with the following materials, group A(distilled water),group B(fluoride-containing toothpaste),group C(tooth mousse),group D(Duraphat), then put into demineralization solution for 1.5 h(37℃), and finally into artificial saliva for 22 h(37℃).The experiment cycle was repeated for 30 days. The scanning and imaging of the samples were completed before treatment and 7, 15 and 30 days after treatment, and the micro hardness of each sample surface was measured by micro hardness tester. Results There was significant difference in the demineralization depth of enamel after 7 days between group A and other groups(P<0.05),but the difference was not significant among group B,C and D(P >0.05).There was no significant difference in the demineralization depth of enamel after 30 days between group C and group D(P >0.05). Micro hardness value of group A was the lowest. The micro hardness value of group C and group D was higher than that of group B(P <0.05).There was no significant difference in micro hardness value between group C and group D(P >0.05). Conclusion OCT can accurately show the demineralization site of enamel. Both GC tooth mousse and Duraphat can effectively inhibit enamel surface demineralization.
Objective To investigate the effect of different preparations on demineralization and remineralization of young permanent teeth by optical coherence tomography(OCT). Methods A total of 60 enamel blocks were prepared from young permanent premolars and randomly divided into 4 groups. The surface of each sample was applied with the following materials, group A(distilled water),group B(fluoride-containing toothpaste),group C(tooth mousse),group D(Duraphat), then put into demineralization solution for 1.5 h(37℃), and finally into artificial saliva for 22 h(37℃).The experiment cycle was repeated for 30 days. The scanning and imaging of the samples were completed before treatment and 7, 15 and 30 days after treatment, and the micro hardness of each sample surface was measured by micro hardness tester. Results There was significant difference in the demineralization depth of enamel after 7 days between group A and other groups(P<0.05),but the difference was not significant among group B,C and D(P >0.05).There was no significant difference in the demineralization depth of enamel after 30 days between group C and group D(P >0.05). Micro hardness value of group A was the lowest. The micro hardness value of group C and group D was higher than that of group B(P <0.05).There was no significant difference in micro hardness value between group C and group D(P >0.05). Conclusion OCT can accurately show the demineralization site of enamel. Both GC tooth mousse and Duraphat can effectively inhibit enamel surface demineralization.
引文
1李静,杜民权,江汉.正畸固定矫治后牙釉质脱矿及其危险因素分析.口腔医学研究,2011,27(1):57-59.
2 Khalaf K.Factors affecting the formation,severity and location of white spot lesions during orthodontic treatment with fixed appliances.J Oral Maxillofac Res,2014,5(1):e4.
3 Marinho VC,Worthington HV,Walsh T,et al.Fluoride varnishes for preventing dental caries in children and adolescents.Cochrane Database Syst Rev,2013,(7):CD002279.
4孟卓,姚晓天,梁燕,等.全光纤光学相干层析牙齿检测系统的解调.传感技术学报,2008,21(11):1858-1861.
5 Nakashima S,Yoshie M,Sano H,et al.Effect of a test dentifrice containing nano-sized calcium carbonate on remineralization of enamel lesions in vitro.J Oral Sci,2009,51(1):69-77.
6 Takahashi N,Nyvad B.The role of bacteria in the caries process:ecological perspectives.J Dent Res,2011,90(3):294-303.
7李燕妮,姚晖,连小丽,等.釉质早期龋的光学相干断层成像与定量研究.华西口腔医学杂志,2015,33(2):121-124.
8刘烨,陈溯,张振庭.含氟酪蛋白磷酸肽-磷酸钙复合物对酸蚀牙釉质显微镜度的影响.北京口腔医学,2012,20(2):74-76.
9时晨,杨清岭,李宝花.渗透树脂联合CPP-ACP对年轻恒牙早期脱矿的影响.临床口腔医学杂志,2017,33(4):208-211.
10彭杰,张敏,张璇,等.正常唾液及氟化物对脱矿年轻恒牙牙釉质的再矿化作用.临床口腔医学杂志,2013,29(8):471-473.
11马京秀,李涢.两种含氟流动树脂对变异链球菌抑制作用的体外实验研究.北京口腔医学,2018,26(3):121-126.
12 Nakagawa H,Sadr A,Shimada Y,et al.Validation of swept source optical coherence tomography(SS-OCT)forthe diagnosis of smooth surface caries invitro.J Dent,2013,41(1):80-89.
13 Staninec M,Douglas SM,Darling CL,et al.Non-destructive clinical assessment of occlusal caries lesions using near IR imaging methods.Lasers Surg Med,2011,43(10):951-959.
14 Sugiura M,Kitasako Y,Sadr A,et al.White spot lesion remineralization by sugar-free chewing gum containing bio-available calcium and fluoride:A double blind randomized controlled trial.JDent,2016,54(7):86-91.
2 Khalaf K.Factors affecting the formation,severity and location of white spot lesions during orthodontic treatment with fixed appliances.J Oral Maxillofac Res,2014,5(1):e4.
3 Marinho VC,Worthington HV,Walsh T,et al.Fluoride varnishes for preventing dental caries in children and adolescents.Cochrane Database Syst Rev,2013,(7):CD002279.
4孟卓,姚晓天,梁燕,等.全光纤光学相干层析牙齿检测系统的解调.传感技术学报,2008,21(11):1858-1861.
5 Nakashima S,Yoshie M,Sano H,et al.Effect of a test dentifrice containing nano-sized calcium carbonate on remineralization of enamel lesions in vitro.J Oral Sci,2009,51(1):69-77.
6 Takahashi N,Nyvad B.The role of bacteria in the caries process:ecological perspectives.J Dent Res,2011,90(3):294-303.
7李燕妮,姚晖,连小丽,等.釉质早期龋的光学相干断层成像与定量研究.华西口腔医学杂志,2015,33(2):121-124.
8刘烨,陈溯,张振庭.含氟酪蛋白磷酸肽-磷酸钙复合物对酸蚀牙釉质显微镜度的影响.北京口腔医学,2012,20(2):74-76.
9时晨,杨清岭,李宝花.渗透树脂联合CPP-ACP对年轻恒牙早期脱矿的影响.临床口腔医学杂志,2017,33(4):208-211.
10彭杰,张敏,张璇,等.正常唾液及氟化物对脱矿年轻恒牙牙釉质的再矿化作用.临床口腔医学杂志,2013,29(8):471-473.
11马京秀,李涢.两种含氟流动树脂对变异链球菌抑制作用的体外实验研究.北京口腔医学,2018,26(3):121-126.
12 Nakagawa H,Sadr A,Shimada Y,et al.Validation of swept source optical coherence tomography(SS-OCT)forthe diagnosis of smooth surface caries invitro.J Dent,2013,41(1):80-89.
13 Staninec M,Douglas SM,Darling CL,et al.Non-destructive clinical assessment of occlusal caries lesions using near IR imaging methods.Lasers Surg Med,2011,43(10):951-959.
14 Sugiura M,Kitasako Y,Sadr A,et al.White spot lesion remineralization by sugar-free chewing gum containing bio-available calcium and fluoride:A double blind randomized controlled trial.JDent,2016,54(7):86-91.