褐牙鲆幼鱼耳石上的外源Sr标记试验
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摘要
为探讨利用外源锶(Sr)在牙鲆耳石上进行人工标记的可行性以及最适浓度,配制了不同浓度SrCl_2·6H_2O溶液(对照组浓度为0,试验组浓度为8、12、16、20mg/L),再将褐牙鲆幼鱼养于各浓度溶液中,进行20d的浸染试验,之后移入正常水体中恢复养殖20d,最后利用X射线电子探针微区分析技术对其耳石Sr标记结果进行确认。结果表明,浸染组鱼耳石上能形成十分明显的红色环带,对照组则无变化。整体上Sr在牙鲆耳石上的沉积量与水体中锶的浓度呈正相关,对8~16mg/L组观察,耳石Sr/Ca的峰值会随浸染浓度增加而增大;但在16~20mg/L组,Sr/Ca的峰值变化不明显。所有试验组幼鱼经恢复养殖后耳石Sr水平均能降到与对照组的一致。以上结果表明,利用外源Sr标记对褐牙鲆这种海生鱼类而言可行,且符合放流标记技术要求,对褐牙鲆而言最佳暴露浓度应以16mg/L为宜。
引文
[1]Begg G A,Campana S E,Fowler A J,et al.Otolith research and application:current directions in innovation and implementation[J].Mar&Fresh Res,2005,56(5):477-483.
[2]Tzeng W N,Tsai Y C.Changes in otolith microchemistry of the Japanese eel,Anguilla japonica,during its migration from the ocean to the river of Taiwan[J].J of Fish Biol,2010,45(4):671-683.
[3]窦硕增.鱼类的耳石信息分析及生活史重建——理论、方法与应用[J].海洋科学集刊,2007(48):93-113.
[4]付自东,谢天明,宋昭彬.鱼类耳石元素指纹研究进展[J].应用与环境生物学报,2007,13(2):278-283.
[5]熊瑛,刘洪波,刘培廷,等.基于耳石元素微化学的江苏吕泗近岸小黄鱼生境履历重建[J].应用生态学报,2014,25(3):836-842.
[6]Townsend D W,Radtke R L,Morrison M A,et al.Recruitment implications of larval herring overwintering distributions in the Gulf of Maine,inferred from a new otolith technique[J].Mar Ecol Prog,1989,55(1):1-13.
[7]Secor D H,Rooker J R,Zlokovitz E,et al.Identification of riverine,estuarine,and coastal contingents of Hudson River striped bass based upon otolith elemental fingerprints[J].Mar Ecol Prog,2001,211(4):245-253.
[8]窦硕增,横内一樹,于鑫,等.基于EPMA的耳石Sr∶Ca比分析及其在鱼类生活履历反演中的应用实例研究[J].海洋与湖沼,2011,42(4):512-520.
[9]陈四清,刘东朴.海水安全优质养殖技术丛书[M].济南:山东科学技术出版社,2008:204-205.
[10]Yang J,Arai T,Liu H,et al.Reconstructing habitat use of Coilia mystus,and Coilia ectenes,of the Yangtze River estuary,and of Coilia ectenes,of Taihu Lake,based on otolith strontium and calcium[J].Journal of Fish Biology,2006,69(4):1120-1135.
[11]杨健,刘洪波.长江口崇明水域鲻鱼耳石元素微化学分析[J].中国水产科学,2010,17(4):853-858.
[12]龚沿东.电子探针(EPMA)简介[J].电子显微学报,2010,29(6):578-580.
[13]李斌之.微区分析技术电子探针的原理应用与溯源[J].计量与测试技术,2011,38(3):26-28.
[14]Zenitani H,Kimura R.Elemental analysis of otoliths of Japanese anchovy:trial to discriminate between Seto Inland Sea and Pacific stock[J].Fisheries Science,2007,73(1):1-8.
[15]陆化杰,陈新军,马金.西北太平洋柔鱼耳石微量元素[J].应用生态学报,2014,25(8):2411-2417.
[16]李秀启,丛旭日,师吉华,等.耳石锶标记在识别鳙(Aristichthys nobilis)放流个体的可行性[J].湖泊科学,2017,29(4):914-922.
[17]姜涛,周昕期,刘洪波,等.鄱阳湖同时存在溯河洄游和淡水定居的刀鲚[J].水产学报,2013,37(2):239-244.
[18]王臣,刘伟,战培荣,等.外源Sr2+在大麻哈鱼胚胎耳石上的沉积[J].应用生态学报,2015,26(10):3189-3194.
[19]Yang J,Jiang T,Liu H.Are there habitat salinity markers of the Sr∶Ca ratio in the otolith of wild diadromous fishes?A literature survey[J].Ichth Res,2011,58(3):291.
[2]Tzeng W N,Tsai Y C.Changes in otolith microchemistry of the Japanese eel,Anguilla japonica,during its migration from the ocean to the river of Taiwan[J].J of Fish Biol,2010,45(4):671-683.
[3]窦硕增.鱼类的耳石信息分析及生活史重建——理论、方法与应用[J].海洋科学集刊,2007(48):93-113.
[4]付自东,谢天明,宋昭彬.鱼类耳石元素指纹研究进展[J].应用与环境生物学报,2007,13(2):278-283.
[5]熊瑛,刘洪波,刘培廷,等.基于耳石元素微化学的江苏吕泗近岸小黄鱼生境履历重建[J].应用生态学报,2014,25(3):836-842.
[6]Townsend D W,Radtke R L,Morrison M A,et al.Recruitment implications of larval herring overwintering distributions in the Gulf of Maine,inferred from a new otolith technique[J].Mar Ecol Prog,1989,55(1):1-13.
[7]Secor D H,Rooker J R,Zlokovitz E,et al.Identification of riverine,estuarine,and coastal contingents of Hudson River striped bass based upon otolith elemental fingerprints[J].Mar Ecol Prog,2001,211(4):245-253.
[8]窦硕增,横内一樹,于鑫,等.基于EPMA的耳石Sr∶Ca比分析及其在鱼类生活履历反演中的应用实例研究[J].海洋与湖沼,2011,42(4):512-520.
[9]陈四清,刘东朴.海水安全优质养殖技术丛书[M].济南:山东科学技术出版社,2008:204-205.
[10]Yang J,Arai T,Liu H,et al.Reconstructing habitat use of Coilia mystus,and Coilia ectenes,of the Yangtze River estuary,and of Coilia ectenes,of Taihu Lake,based on otolith strontium and calcium[J].Journal of Fish Biology,2006,69(4):1120-1135.
[11]杨健,刘洪波.长江口崇明水域鲻鱼耳石元素微化学分析[J].中国水产科学,2010,17(4):853-858.
[12]龚沿东.电子探针(EPMA)简介[J].电子显微学报,2010,29(6):578-580.
[13]李斌之.微区分析技术电子探针的原理应用与溯源[J].计量与测试技术,2011,38(3):26-28.
[14]Zenitani H,Kimura R.Elemental analysis of otoliths of Japanese anchovy:trial to discriminate between Seto Inland Sea and Pacific stock[J].Fisheries Science,2007,73(1):1-8.
[15]陆化杰,陈新军,马金.西北太平洋柔鱼耳石微量元素[J].应用生态学报,2014,25(8):2411-2417.
[16]李秀启,丛旭日,师吉华,等.耳石锶标记在识别鳙(Aristichthys nobilis)放流个体的可行性[J].湖泊科学,2017,29(4):914-922.
[17]姜涛,周昕期,刘洪波,等.鄱阳湖同时存在溯河洄游和淡水定居的刀鲚[J].水产学报,2013,37(2):239-244.
[18]王臣,刘伟,战培荣,等.外源Sr2+在大麻哈鱼胚胎耳石上的沉积[J].应用生态学报,2015,26(10):3189-3194.
[19]Yang J,Jiang T,Liu H.Are there habitat salinity markers of the Sr∶Ca ratio in the otolith of wild diadromous fishes?A literature survey[J].Ichth Res,2011,58(3):291.